Transmutation elements produced by neutron irradiation significantly affect the irradiation response of materials,yet they are rarely considered in contemporary studies of ion irradiation to simulate neutron irradiati...Transmutation elements produced by neutron irradiation significantly affect the irradiation response of materials,yet they are rarely considered in contemporary studies of ion irradiation to simulate neutron irradiation damage.Here,we present the first in-situ investigation of the effects of transmutation silicon on irradiation defect evolution and corresponding mechanisms in Al-Mg alloys in TEM,using Al+and Si+irradiation.The evolution of dislocation loops under Si+irradiation was closely related to the implanted Si concentration,showing a distinct inhibition threshold at 0.05 at.%.The implanted Si increased the elemental complexity of the matrix and hindered the transition from faulted loop to perfect loop,which should be attributed to the simultaneous effect from both(1)interaction between Si interstitials and point defects and(2)competition between Si atoms and solute-Mg atoms for vacancies and the formation of atomic Mg-Si clusters.These findings revealed the key factors in irradiation-induced dislocation networks in aluminum alloys and the specific role of silicon,and indicated that the effects of transmutation elements needed to be fully considered when using ions to study the irradiation behavior of materials.展开更多
Annealing treatment is an effective strategy to enhance the comprehensive properties of Mg-8Li-3Al-2Zn(LAZ832)alloy,where the cooling rate plays a decisive role in tailoring microstructure and performance.This study s...Annealing treatment is an effective strategy to enhance the comprehensive properties of Mg-8Li-3Al-2Zn(LAZ832)alloy,where the cooling rate plays a decisive role in tailoring microstructure and performance.This study systematically investigates the effects of cooling rates,controlled via water quenching(WC),air cooling(AC),and furnace cooling(FC),on the phase evolution,mechanical properties,and corrosion resistance of LAZ832.The annealed microstructure consists ofα-Mg,β-Li,AlLi,and MgLi_(2)Al phases,and the volume fraction of Al-Li phases(AlLi and MgLi_(2)Al)increases as the cooling rate decreases.Strengthening mechanisms are dominated by solid solution strengthening,driven by the dissolution of Al and Zn atoms into the matrix,which significantly enhances tensile strength.However,excessive solute content leads to a marked decline in ductility.Scanning probe microscope(SPM)reveals an elevated work function due to the dissolution of Al and Zn atoms into the matrix phase,correlating with improved corrosion resistance.Comprehensive analysis demonstrates that air cooling achieves an optimal balance between tensile strength,ductility,and corrosion resistance,outperforming furnace-cooled samples and offering a pragmatic compromise compared to water-quenched specimens with higher strength but brittle failure.These findings establish a robust framework for designing LAZ832 alloys with tailored microstructures and multi-property optimization,advancing their application in lightweight engineering fields.展开更多
Wnt signaling pathways,including the canonical Wnt/β-catenin pathway,planar cell polarity pathway,and Wnt/Ca2+signaling pathway,play important roles in neural development during embryonic stages.The DVL genes encode ...Wnt signaling pathways,including the canonical Wnt/β-catenin pathway,planar cell polarity pathway,and Wnt/Ca2+signaling pathway,play important roles in neural development during embryonic stages.The DVL genes encode the hub proteins for Wnt signaling pathways.The mutations in DVL2 and DVL3 were identified from patients with neural tube defects(NTDs),but their functions in the pathogenesis of human neural diseases remain elusive.Here,we sequenced the coding regions of three DVL genes in 176 stillborn or miscarried fetuses with NTDs or Dandy-Walker malformation(DWM)and 480 adult controls from a Han Chinese population.Four rare mutations were identified:DVL1 p.R558 H,DVL1 p.R606 C,DVL2 p.R633 W,and DVL3 p.R222 Q.To assess the effect of these mutations on NTDs and DWM,various functional analyses such as luciferase reporter assay,stress fiber formation,and in vivo teratogenic assay were performed.The results showed that the DVL2 p.R633 W mutation destabilized DVL2 protein and upregulated activities for all three Wnt signalings(Wnt/β-catenin signaling,Wnt/planar cell polarity signaling,and Wnt/Ca2+signaling)in mammalian cells.In contrast,DVL1 mutants(DVL1 p.R558 H and DVL1 p.R606 C)decreased canonical Wnt/β-catenin signaling but increased the activity of Wnt/Ca2+signaling,and DVL3 p.R222 Q only decreased the activity of Wnt/Ca2+signaling.We also found that only the DVL2 p.R633 W mutant displayed more severe teratogenicity in zebrafish embryos than wild-type DVL2.Our study demonstrates that these four rare DVL mutations,especially DVL2 p.R633 W,may contribute to human neural diseases such as NTDs and DWM by obstructing Wnt signaling pathways.展开更多
Dynamic wetting plays an important role in the physics of multiphase flow, and has a significant influence on many industrial and geotechnical applications. In this work, a modified smoothed particle hydrodynamics (SP...Dynamic wetting plays an important role in the physics of multiphase flow, and has a significant influence on many industrial and geotechnical applications. In this work, a modified smoothed particle hydrodynamics (SPH) model is employed to simulate surface tension, contact angle and dynamic wetting effects at meso-scale. The wetting and dewetting phenomena are simulated in a capillary tube, where the liquid particles are raised or withdrawn by a shifting substrate. The SPH model is modified by introducing a newly developed viscous force formulation at the liquid-solid interface to reproduce the rate-dependent behaviour of the moving contact line. Dynamic contact angle simulations with the interfacial viscous force are conducted to verify the effectiveness and accuracy of this new formulation. In addition, the influence of interfacial viscous forces with different magnitude on the contact angle dynamics is examined by empirical power-law correlations;the derived constants suggest that the dynamic contact angle changes monotonically with the interfacial viscous force. The simulation results are consistent with experimental observations and theoretical predictions, implying that the interfacial viscous force can be associated with the slip length of flow and the microscopic surface roughness. This work demonstrates that the modified SPH model can successfully account for the rate-dependent effects of a moving contact line, and can be used for realistic multiphase flow simulations under dynamic conditions.展开更多
This study was conducted to investigate the drug sensitivity of Escherichia coli isolated from sheep, providing data reference for clinical medication. Fifty-four samples were collected from a certain sheep farm in Ya...This study was conducted to investigate the drug sensitivity of Escherichia coli isolated from sheep, providing data reference for clinical medication. Fifty-four samples were collected from a certain sheep farm in Yangling, Shaanxi Province for E. coli isolation and identification, and K-B disk diffusion method was used to carry out the dug sensitivity test of 40 E. coli to the eight antimicrobials of spectinomycin, amikacin, tobramycin, enrofloxacin, nalidixic acid, levofloxacin, salafloxacin and ciprofloxacin. The results showed that there were 40 positive E. coli strains and the isolation rate was 74.7%. The resistance rates of the 40 E. coli to spectinomycin, amikacin,tobramycin and nalidixic acid were 85%, 95%, 97.5% and 87.5%, respectively, while the resistance rate to enrofloxacin, levofloxacin, salafloxacin and ciprofloxacin were relatively low and the resistance rates were 35%, 20%, 25% and 17.5%, respectively. The isolates showed different levels of drug resistance to eight antimicrobials and the highest resistance rate was 97.5%. It reminds that the sheep farm should strictly control the dosage according to the antimicrobial sensitive data in clinic.展开更多
It has become clear that the extant vertebrates are divided into three major groups, that is, hagfishes, lampreys, and jawed vertebrates. Morphological and molecular studies, however, have resulted in conflicting view...It has become clear that the extant vertebrates are divided into three major groups, that is, hagfishes, lampreys, and jawed vertebrates. Morphological and molecular studies, however, have resulted in conflicting views with regard to their interrelationships. To clarify the phylogenetic relationships between them, 48 orthologous protein-coding gene families were analyzed. Even as the analysis of 34 nuclear gene families supported the monophyly of cyclostomes, the analysis of 14 mitochondrial gene families suggested a closer relationship between lampreys and gnathostomes compared to hagfishes. Lampreys were sister group of gnathostomes. The results of this study supported the cyclostomes. Choice of outgroup, tree-making methods, and software may affect the phylogenetic prediction, which may have caused much debate over the subject. Development of new methods for tackling such problems is still necessary.展开更多
Objective： To investigate the role of tissue factor （TF） in chemotherapeutic reagent - induced apoptosis on human glioblastoma and explore its mechanism. Methods： The expression of TF was examined by Western blott...Objective： To investigate the role of tissue factor （TF） in chemotherapeutic reagent - induced apoptosis on human glioblastoma and explore its mechanism. Methods： The expression of TF was examined by Western blotting. The cytotoxicity of doxorubicin was determined by WST assay. The activation of Caspase-3 and PARP induced by adoxorubicin were tested by Western blotting. Results： Human glioblastoma cell line U373MG expressed high level of TF while LN-229 was with low-TF level. The chemotherapeutic reagent doxorubicin revealed stronger cytotoxic effect on high-TF U373MG cells than IowoTF LN-229 cells. Enforced strong expression of TF was achieved by transfection of TF-pcDNA3 combinant on LN-229 cells in a dose-dependent manner. Enforced TF expression in transfected LN-229 cells not only impaired the doxorubicin-induced cleavage of Caspase-3 and PARP, but also inhibited the cytotoxic effect of doxorubicin. Furthermore, activation of Akt was strong in high-TF U373MG cells but weak in IowoTF LN-229 cells. Incubation of factor VII （FVII） with enforced TF-expressing LN-229 cells increased the phosphorylaUon of Akt in a time-dependent manner. Conclusion： These results suggest that over-expression of TF on glioblastoma could inhibit doxorubicin-induced apoptosis. Interaction of FVII and TF activates the downstream PI3K/Akt pathway. Tumor-derived over-expression of TF might play a role in chemotherapy resistance in glioblastoma, at lest in part, by activating PI3K/Akt-mediated survival and anti-apoptotic mechanism through the interaction of TF/FVII signaling.展开更多
Gastric ulcer(GU)is a common digestive system disease.Acupuncture,as one of the external treatments of traditional Chinese medicine(TCM),has the characteristics of multi-target,multi-pathway and multi-level action in ...Gastric ulcer(GU)is a common digestive system disease.Acupuncture,as one of the external treatments of traditional Chinese medicine(TCM),has the characteristics of multi-target,multi-pathway and multi-level action in the treatment of GU.The relationship between meridian points and Zang-fu is an important part of the theory of TCM,which is crucial for the diagnosis and treatment of diseases.There is an external and internal link between acupoints and Zang-fu.The pathological reaction of Zang-fu can manifest as acupoint sensitization,while stimulation of acupoints can play a therapeutic role in the internal Zang-fu.Therefore,the acupoint has the functions of reflecting and treating diseases.This review explores the tender points on the body surface of patients with GU and the rules of acupoint selection.In addition,Zusanli(ST36),as one of the most used acupoints of the stomach meridian,was selected to show the mechanisms behind acupoint stimulation in the treatment of GU in greater detail,specifically in the well-studied model of the stress GU(SGU).Hence,the mechanisms of acupuncture at ST36 and points commonly used in combination with ST36 to treat SGU are discussed further.Treatment effects can be achieved through anti-inflammatory and antioxidant activities,gastric mucosal injury repair,and interaction with the brain-gut axis.In summary,this review provides evidence for a comprehensive understanding of the phenomena and mechanism of acupoint functions for GU.展开更多
The lithium-oxygen battery(LOB)is a promising source of green energy due to its energy density.However,the development of this technology is limited by the insoluble discharge product it produces.In this work,a cathod...The lithium-oxygen battery(LOB)is a promising source of green energy due to its energy density.However,the development of this technology is limited by the insoluble discharge product it produces.In this work,a cathode material with a p-n heterostructure of polyaniline(PANI)/ZnS is prepared to trap visible light,utilizing a ZnS quantum dot(ZnS QD)network to form a large number of photogenerated electron–hole pairs,thus promoting the generation and decomposition of Li_(2)O_(2).The prepared PANI/ZnS has an ultra-low overpotential of 0.06 V under illumination.Furthermore,density functional theory theoretical calculation has demonstrated the ability of the heterostructures to adsorb oxygen-containing intermediates,which not only facilitates the growth of Li_(2)O_(2),but also reduces the reaction energy required to decompose Li_(2)O_(2).The present work provides a solution to the problem of insolubility of discharge products in photo-assisted LOB.展开更多
Objective: This study investigates the auxiliary role of resting-state electroencephalography (EEG) in the clinical diagnosis of attention-deficit hyperactivity disorder (ADHD) using machine learning techniques. Metho...Objective: This study investigates the auxiliary role of resting-state electroencephalography (EEG) in the clinical diagnosis of attention-deficit hyperactivity disorder (ADHD) using machine learning techniques. Methods: Resting-state EEG recordings were obtained from 57 children, comprising 28 typically developing children and 29 children diagnosed with ADHD. The EEG signal data from both groups were analyzed. To ensure analytical accuracy, artifacts and noise in the EEG signals were removed using the EEGLAB toolbox within the MATLAB environment. Following preprocessing, a comparative analysis was conducted using various ensemble learning algorithms, including AdaBoost, GBM, LightGBM, RF, XGB, and CatBoost. Model performance was systematically evaluated and optimized, validating the superior efficacy of ensemble learning approaches in identifying ADHD. Conclusion: Applying machine learning techniques to extract features from resting-state EEG signals enabled the development of effective ensemble learning models. Differential entropy and energy features across multiple frequency bands proved particularly valuable for these models. This approach significantly enhances the detection rate of ADHD in children, demonstrating high diagnostic efficacy and sensitivity, and providing a promising tool for clinical application.展开更多
[Objectives]To explore the effectiveness of integrating ideological and political education based on the Nightingale spirit into the clinical practice curriculum for nursing interns.[Methods]A total of 90 questionnair...[Objectives]To explore the effectiveness of integrating ideological and political education based on the Nightingale spirit into the clinical practice curriculum for nursing interns.[Methods]A total of 90 questionnaires were distributed and analyzed among nursing interns and patients to evaluate multiple dimensions,including the interns satisfaction with the internship,mastery of clinical knowledge,ideological and political literacy,professional ethics,and social responsibility.[Results]The overall internship satisfaction of nursing interns reached 98.9%,indicating that the course design and teaching model were widely recognized by students.Secondly,the interns demonstrated excellent mastery of clinical knowledge,with 94.0%of students stating they had fully grasped the clinical knowledge taught during the internship,an increase of 18.6%compared to the previous cohort.In terms of ideological and political literacy,98%of students had a better understanding and application of the Nightingale spirit,an increase of 16.2%compared to the previous cohort.Additionally,the sense of professional identity was significantly enhanced,with 95.8%of students showing a stronger sense of identity and responsibility towards the nursing profession,an increase of 14.0%compared to the previous cohort.Patients also gave high evaluations of the interns performance,with 98.8%expressing satisfaction with the students attitude and performance in nursing care.[Conclusions]The curriculum model based on the integration of Nightingale spirit has effectively improved the professional quality,ideological and political quality and professional identity of nursing interns,and has been highly recognized by patients.It not only provides strong support for the practice of combining ideological and political work with professional courses in nursing education,but also provides a reference for the optimization of clinical practice teaching mode of nursing interns in the future.展开更多
The stability and safety of aqueous aluminum metal batteries(AAMBs)have garnered an enormous amount of attention.However,severe corrosion during cycling and inefficient deposition behavior at the Al anode hinder their...The stability and safety of aqueous aluminum metal batteries(AAMBs)have garnered an enormous amount of attention.However,severe corrosion during cycling and inefficient deposition behavior at the Al anode hinder their application.In this paper,CeCl_(3)was selected as a water inhibition additive to achieve active water confinement,which dramatically improved the corrosion behavior and prolonged the cycle life of the Al electrode.A combination of spectroscopic characterization and computational analysis showed that Cl^(-)breaks hydrogen bonds in the electrolyte and has higher adsorption energy on Al,thus inhibiting water-induced corrosion.Meanwhile,Ce^(3+)has a stronger affinity for Al than H_(2)O,thus promoting the formation of the surface protective layer.Cl^(-)in the modified electrolyte results in less hydration around Al^(3+).Due to the excellent water inhibition effect of CeCl_(3),the corrosion phenomenon of Al electrodes was significantly improved,and the dominant growth of the Al(111)crystal plane was achieved.Al//Prussian blue analogue(PBA)full cells with CeCl_(3)exhibit significantly improved voltage polarization(0.342 V),cycle life(550 cycles),discharge specific capacity(112 mAh g^(-1)),and selfdischarge behavior(87.97%).The Ce^(3+)in the additive is also able to be co-intercalated into the PBA with Al^(3+),improving the stability of the PBA.However,the conductivity reduction of this strategy at higher concentrations needs to be further addressed.Compared with organic electrolyte and molten salt systems,the cycle life of the aqueous electrolyte in this work still falls short.This modification method paves the way for further development of efficient AAMBs.展开更多
Objective:To observe the clinical efficacy of the method of softening the liver,harmonizing the spleen,and unblocking the orifices in the treatment of Xiaoke Neizhang with Qi and Yin deficiency type.Methods:Seventy-tw...Objective:To observe the clinical efficacy of the method of softening the liver,harmonizing the spleen,and unblocking the orifices in the treatment of Xiaoke Neizhang with Qi and Yin deficiency type.Methods:Seventy-two patients with Xiaoke Neizhang of qi and yin deficiency type were selected from March 2024 to May 2025 and divided into a treatment group and a control group with 36 cases each according to the random number table method.The treatment group was treated with the method of softening the liver,harmonizing the spleen,and unblocking the orifices,which included oral administration of Softening the Liver and Harmonizing the Spleen Pills combined with iontophoresis of Xuesaitong Injection.The control group was treated with oral administration of calcium dobesilate capsules.Both groups were treated for 2 courses,and multiple indicators such as visual acuity,fundus condition,and TCM syndrome scores were compared between the two groups.Results:The total effective rate of the treatment group was higher than that of the control group.There were significant differences in visual acuity,fundus condition,and TCM syndrome scores between and within the groups(P<0.05).Conclusion:The method of softening the liver,harmonizing the spleen,and unblocking the orifices has a definite clinical effect in the treatment of Xiaoke Neizhang with Qi and Yin deficiency type.It has a direct effect,is painless,easy for patients to accept,and has high clinical application value.展开更多
It is well known that cationic polymers have excellent antimicrobial capacity accompanied with high biotoxicity,to reduce biotoxicity needs to decrease the number of cationic groups on polymers,which will influence an...It is well known that cationic polymers have excellent antimicrobial capacity accompanied with high biotoxicity,to reduce biotoxicity needs to decrease the number of cationic groups on polymers,which will influence antimicrobial activity.It is necessary to design a cationic polymer mimic natural antimicrobial peptide with excellent antibacterial activity and low toxicity to solve the above dilemma.Here,we designed and prepared a series of cationic poly(β-amino ester)s(PBAEs)with different cationic contents,and introducing hydrophobic alkyl chain to adjust the balance between antimicrobial activity and biotoxicity to obtain an ideal antimicrobial polymer.The optimum one of synthesized PBAE(hydrophilic cationic monomer:hydrophobic monomer=5:5)was screened by testing cytotoxicity and minimum inhibitory concentration(MIC),which can effectively kill S.aureus and E.coli with PBAE concentration of15μg/m L by a spread plate bacteriostatic method and dead and alive staining test.The way of PBAE killing bacterial was destroying the membrane like natural antimicrobial peptide observed by scanning electron microscopy(SEM).In addition,PBAE did not exhibit hemolysis and cytotoxicity.In particular,from the result of animal tests,the PBAE was able to promote healing of infected wounds from removing mature S.aureus and E.coli on the surface of infected wound.As a result,our work offers a viable approach for designing antimicrobial materials,highlighting the significant potential of PBAE polymers in the field of biomedical materials.展开更多
Objective:Anillin(ANLN)is considered an oncogene in various cancers,but its effect on cervical cancer remains poorly understood.Hence,this study aimed to describe the action of ANLN on cervical cancer development and ...Objective:Anillin(ANLN)is considered an oncogene in various cancers,but its effect on cervical cancer remains poorly understood.Hence,this study aimed to describe the action of ANLN on cervical cancer development and investigate the potential mechanism.Methods:Analysis of ANLN expression and its association with survival in carcinoma and endocervical adenocarcinoma(CESC)patients based on GEO and UALCAN databases.The tumor and adjacent normal tissues of 100 cervical cancer cases were harvested to detect the ANLN expression and explore its relationship with patient survival.Cell proliferation,apoptosis,migration,and invasion were measured by utilizing 5-ethynyl-2′-deoxyuridine(EdU)staining,Flow cytometry,and Transwell assay,respectively.ANLN andWnt expression were analyzed by RT-qPCR andWestern Blot.Results:ANLN was significantly elevated in tumor tissues,and cervical cancer cases with high ANLN expression exhibited poor survival and high dead proportion.Besides,ANLN induced cervical cancer cell proliferation,migration,and invasion and restrained cell apoptosis.In addition,ANLN promoted Wnt/β-catenin pathway activation.Furthermore,ANLN accelerated cell aggressive behaviors and suppressed cell apoptosis via activating the Wnt/β-catenin signaling in cervical cancer.Conclusion:ANLN was enhanced in cervical cancer tissues and related to poor prognosis.ANLN accelerated cervical cancer cell aggressive behaviors and suppressed cell apoptosis via activating theWnt/β-catenin pathway.展开更多
Benefiting from the widespread potential applications in the era of the Internet of Thing and metaverse,triboelectric and piezoelectric nanogenerators(TENG&PENG)have attracted considerably increasing attention.The...Benefiting from the widespread potential applications in the era of the Internet of Thing and metaverse,triboelectric and piezoelectric nanogenerators(TENG&PENG)have attracted considerably increasing attention.Their outstanding characteristics,such as self-powered ability,high output performance,integration compatibility,cost-effectiveness,simple configurations,and versatile operation modes,could effectively expand the lifetime of vastly distributed wearable,implantable,and environmental devices,eventually achieving self-sustainable,maintenance-free,and reliable systems.However,current triboelectric/piezoelectric based active(i.e.self-powered)sensors still encounter serious bottlenecks in continuous monitoring and multimodal applications due to their intrinsic limitations of monomodal kinetic response and discontinuous transient output.This work systematically summarizes and evaluates the recent research endeavors to address the above challenges,with detailed discussions on the challenge origins,designing strategies,device performance,and corresponding diverse applications.Finally,conclusions and outlook regarding the research gap in self-powered continuous multimodal monitoring systems are provided,proposing the necessity of future research development in this field.展开更多
Sonodynamic therapy(SDT)is garnering considerable attention as a promising treatment for deep-seated tumors because of its strong tissue penetration ability,non-invasiveness,and controllability.However,the SDT efficie...Sonodynamic therapy(SDT)is garnering considerable attention as a promising treatment for deep-seated tumors because of its strong tissue penetration ability,non-invasiveness,and controllability.However,the SDT efficiency of traditional sonosensitizers including porphyrins and their derivatives are limited due to their poor water dissolubility,high aggregation,and low reactive oxygen species(ROS)production efficiency.Consequently,it is crucial to develop novel sonosensitizers with high yields of ROS,outstanding water solubility,and good biocompatibility.Herein,we constructed a new platform for SDT based on unimolecular porphyrin derivatives OPV-C_(3)-TPP.The probe OPV-C_(3)-TPP was synthesized by covalently linking conjugated oligomers(OPV)with 5,10,15,20-tetra(4-aminophenyl)porphyrin(TAPP).The introduction of OPV greatly improves the water solubility of the porphyrins and reduces the self-aggregation of the porphyrins.In addition,OPV-C_(3)-TPP has good intramolecular energy transfer efficiency,thus enhancing the yield of ROS.The experimental results show that OPV-C_(3)-TPP exhibits excellent ROS generation capacity under ultrasound(US)irradiation,which leads to apoptosis and necrosis of tumor cells.In vivo tumor growth is also significantly inhibited in the OPV-C_(3)-TPP t US group,exhibiting better SDT effects than TAPP.Therefore,the unimolecular OPV-C_(3)-TPP can be used as a potential sonosensitizer,providing a promising SDT for deep-tissue tumors.展开更多
The concurrence of primary sclerosing cholangitis(PSC)and inflammatory bowel disease(IBD)presents a therapeutic challenge,often necessitating liver transplantation in severe cases.Paeoniflorin(PAE),known for its immun...The concurrence of primary sclerosing cholangitis(PSC)and inflammatory bowel disease(IBD)presents a therapeutic challenge,often necessitating liver transplantation in severe cases.Paeoniflorin(PAE),known for its immunomodulatory and anti-inflammatory properties but with very high-water solubility and low permeability,is formulated into a paeoniflorin/phospholipid complex microemulsion(PAE-ME)to enhance its delivery in this study.It demonstrated the PAE-ME's macrophage-regulating ability to repolarize the pro-inflammatory M1 subtype to the anti-inflammatory M2 type and reduce inflammatory cytokine release.In a PSC-IBD mouse model,PAE-ME alleviated the symptoms and regulated bile acid balance.Given the close connection and crosstalk between the liver and intestine,PAE-ME yielded a synergistic therapeutic effect on both the liver and intestinal lesions.These findings suggest a promising translational approach for complex comorbidities by acting on the liver-gut axis.展开更多
Shape memory alloys(SMAs)are smart materials with superelasticity originating from a reversible stressinduced martensitic transformation(MT)accompanied by a significant electrical resistance change.However,the stress-...Shape memory alloys(SMAs)are smart materials with superelasticity originating from a reversible stressinduced martensitic transformation(MT)accompanied by a significant electrical resistance change.However,the stress-strain and resistance-stress relationships of typical NiTi wires are non-linear due to the stress plateau during the stress-induced MT.This limits the usage of these materials as pressure sensors.Herein,we propose a high-strength flexible sensor based on superelastic NiTi wires that achieves near-linear mechanical and electrical responses through a low-cost double-braided strategy.This microarchitectured strategy reduces or even eliminates stress plateau and it is demonstrated that the phase transformation of microfilaments can be controlled:regions with localized stress undergo the MT first,which is successively followed by the rest of the microfilament.This structure-dependent MT characteristic exhibits slim-hysteresis superelasticity and tunable low stiffness,and the braided wire shows improved flexibility.The double-braided NiTi microfilaments exhibit stable electrical properties and repeatability under approximately 600 MPa(8%strain)and can maintain stability over a wide temperature range(303-403 K).Moreover,a cross-grid flexible woven sensor array textile based on microfilaments is further developed to detect pressure distribution.This work provides insight into the design and application of SMAs in the field of flexible and functional fiber.展开更多
基金supported by the National Science Fund for Distinguished Young Scholar of China(No.12225506)the National Natural Science Foundation of China(No.U2341256 and U24B2013).
文摘Transmutation elements produced by neutron irradiation significantly affect the irradiation response of materials,yet they are rarely considered in contemporary studies of ion irradiation to simulate neutron irradiation damage.Here,we present the first in-situ investigation of the effects of transmutation silicon on irradiation defect evolution and corresponding mechanisms in Al-Mg alloys in TEM,using Al+and Si+irradiation.The evolution of dislocation loops under Si+irradiation was closely related to the implanted Si concentration,showing a distinct inhibition threshold at 0.05 at.%.The implanted Si increased the elemental complexity of the matrix and hindered the transition from faulted loop to perfect loop,which should be attributed to the simultaneous effect from both(1)interaction between Si interstitials and point defects and(2)competition between Si atoms and solute-Mg atoms for vacancies and the formation of atomic Mg-Si clusters.These findings revealed the key factors in irradiation-induced dislocation networks in aluminum alloys and the specific role of silicon,and indicated that the effects of transmutation elements needed to be fully considered when using ions to study the irradiation behavior of materials.
基金the financial support of the Scientific Research Starting Foundation of Anhui Polytechnic University of China(Grant No.2200YQQ049)the Excellent Scientific Research and Innovation Teams of Anhui Province,China(Grant No.2022AH010059).
文摘Annealing treatment is an effective strategy to enhance the comprehensive properties of Mg-8Li-3Al-2Zn(LAZ832)alloy,where the cooling rate plays a decisive role in tailoring microstructure and performance.This study systematically investigates the effects of cooling rates,controlled via water quenching(WC),air cooling(AC),and furnace cooling(FC),on the phase evolution,mechanical properties,and corrosion resistance of LAZ832.The annealed microstructure consists ofα-Mg,β-Li,AlLi,and MgLi_(2)Al phases,and the volume fraction of Al-Li phases(AlLi and MgLi_(2)Al)increases as the cooling rate decreases.Strengthening mechanisms are dominated by solid solution strengthening,driven by the dissolution of Al and Zn atoms into the matrix,which significantly enhances tensile strength.However,excessive solute content leads to a marked decline in ductility.Scanning probe microscope(SPM)reveals an elevated work function due to the dissolution of Al and Zn atoms into the matrix phase,correlating with improved corrosion resistance.Comprehensive analysis demonstrates that air cooling achieves an optimal balance between tensile strength,ductility,and corrosion resistance,outperforming furnace-cooled samples and offering a pragmatic compromise compared to water-quenched specimens with higher strength but brittle failure.These findings establish a robust framework for designing LAZ832 alloys with tailored microstructures and multi-property optimization,advancing their application in lightweight engineering fields.
基金supported by Research and Development Program of China(2016YFC1000500)to H.W.and W.T.the National Natural Science Foundation of China(81430005,31521003,31771669)to H.W.and(31601029)to Y.G.the Commission for Science and Technology of Shanghai Municipality(17JC1400902)to H.W.
文摘Wnt signaling pathways,including the canonical Wnt/β-catenin pathway,planar cell polarity pathway,and Wnt/Ca2+signaling pathway,play important roles in neural development during embryonic stages.The DVL genes encode the hub proteins for Wnt signaling pathways.The mutations in DVL2 and DVL3 were identified from patients with neural tube defects(NTDs),but their functions in the pathogenesis of human neural diseases remain elusive.Here,we sequenced the coding regions of three DVL genes in 176 stillborn or miscarried fetuses with NTDs or Dandy-Walker malformation(DWM)and 480 adult controls from a Han Chinese population.Four rare mutations were identified:DVL1 p.R558 H,DVL1 p.R606 C,DVL2 p.R633 W,and DVL3 p.R222 Q.To assess the effect of these mutations on NTDs and DWM,various functional analyses such as luciferase reporter assay,stress fiber formation,and in vivo teratogenic assay were performed.The results showed that the DVL2 p.R633 W mutation destabilized DVL2 protein and upregulated activities for all three Wnt signalings(Wnt/β-catenin signaling,Wnt/planar cell polarity signaling,and Wnt/Ca2+signaling)in mammalian cells.In contrast,DVL1 mutants(DVL1 p.R558 H and DVL1 p.R606 C)decreased canonical Wnt/β-catenin signaling but increased the activity of Wnt/Ca2+signaling,and DVL3 p.R222 Q only decreased the activity of Wnt/Ca2+signaling.We also found that only the DVL2 p.R633 W mutant displayed more severe teratogenicity in zebrafish embryos than wild-type DVL2.Our study demonstrates that these four rare DVL mutations,especially DVL2 p.R633 W,may contribute to human neural diseases such as NTDs and DWM by obstructing Wnt signaling pathways.
文摘Dynamic wetting plays an important role in the physics of multiphase flow, and has a significant influence on many industrial and geotechnical applications. In this work, a modified smoothed particle hydrodynamics (SPH) model is employed to simulate surface tension, contact angle and dynamic wetting effects at meso-scale. The wetting and dewetting phenomena are simulated in a capillary tube, where the liquid particles are raised or withdrawn by a shifting substrate. The SPH model is modified by introducing a newly developed viscous force formulation at the liquid-solid interface to reproduce the rate-dependent behaviour of the moving contact line. Dynamic contact angle simulations with the interfacial viscous force are conducted to verify the effectiveness and accuracy of this new formulation. In addition, the influence of interfacial viscous forces with different magnitude on the contact angle dynamics is examined by empirical power-law correlations;the derived constants suggest that the dynamic contact angle changes monotonically with the interfacial viscous force. The simulation results are consistent with experimental observations and theoretical predictions, implying that the interfacial viscous force can be associated with the slip length of flow and the microscopic surface roughness. This work demonstrates that the modified SPH model can successfully account for the rate-dependent effects of a moving contact line, and can be used for realistic multiphase flow simulations under dynamic conditions.
基金Supported by the Fundamental Research Funds for the Central Universities:the Young Teachers Fund of Southwest Minzu University(2017NZYQN38)Innovative Scientific Research Project for Postgraduates of Southwest Minzu University(CX2018SZ42)
文摘This study was conducted to investigate the drug sensitivity of Escherichia coli isolated from sheep, providing data reference for clinical medication. Fifty-four samples were collected from a certain sheep farm in Yangling, Shaanxi Province for E. coli isolation and identification, and K-B disk diffusion method was used to carry out the dug sensitivity test of 40 E. coli to the eight antimicrobials of spectinomycin, amikacin, tobramycin, enrofloxacin, nalidixic acid, levofloxacin, salafloxacin and ciprofloxacin. The results showed that there were 40 positive E. coli strains and the isolation rate was 74.7%. The resistance rates of the 40 E. coli to spectinomycin, amikacin,tobramycin and nalidixic acid were 85%, 95%, 97.5% and 87.5%, respectively, while the resistance rate to enrofloxacin, levofloxacin, salafloxacin and ciprofloxacin were relatively low and the resistance rates were 35%, 20%, 25% and 17.5%, respectively. The isolates showed different levels of drug resistance to eight antimicrobials and the highest resistance rate was 97.5%. It reminds that the sheep farm should strictly control the dosage according to the antimicrobial sensitive data in clinic.
基金the National Natural Science Foundation of China （No. 60575005） the Natural Science Foundation of Liaoning Province （No. 20072152）+3 种基金 Key Project of Science and Technology of the Educational Ministry of China （No. 206032） the Science and Technology Project of Dalian City （No. 2006E11SF068） the National High Technology Research and Development Program of China （No. 2007AA09ZA28） the National Basic Research Program of China （No. 2007CB815802）.
文摘It has become clear that the extant vertebrates are divided into three major groups, that is, hagfishes, lampreys, and jawed vertebrates. Morphological and molecular studies, however, have resulted in conflicting views with regard to their interrelationships. To clarify the phylogenetic relationships between them, 48 orthologous protein-coding gene families were analyzed. Even as the analysis of 34 nuclear gene families supported the monophyly of cyclostomes, the analysis of 14 mitochondrial gene families suggested a closer relationship between lampreys and gnathostomes compared to hagfishes. Lampreys were sister group of gnathostomes. The results of this study supported the cyclostomes. Choice of outgroup, tree-making methods, and software may affect the phylogenetic prediction, which may have caused much debate over the subject. Development of new methods for tackling such problems is still necessary.
文摘Objective： To investigate the role of tissue factor （TF） in chemotherapeutic reagent - induced apoptosis on human glioblastoma and explore its mechanism. Methods： The expression of TF was examined by Western blotting. The cytotoxicity of doxorubicin was determined by WST assay. The activation of Caspase-3 and PARP induced by adoxorubicin were tested by Western blotting. Results： Human glioblastoma cell line U373MG expressed high level of TF while LN-229 was with low-TF level. The chemotherapeutic reagent doxorubicin revealed stronger cytotoxic effect on high-TF U373MG cells than IowoTF LN-229 cells. Enforced strong expression of TF was achieved by transfection of TF-pcDNA3 combinant on LN-229 cells in a dose-dependent manner. Enforced TF expression in transfected LN-229 cells not only impaired the doxorubicin-induced cleavage of Caspase-3 and PARP, but also inhibited the cytotoxic effect of doxorubicin. Furthermore, activation of Akt was strong in high-TF U373MG cells but weak in IowoTF LN-229 cells. Incubation of factor VII （FVII） with enforced TF-expressing LN-229 cells increased the phosphorylaUon of Akt in a time-dependent manner. Conclusion： These results suggest that over-expression of TF on glioblastoma could inhibit doxorubicin-induced apoptosis. Interaction of FVII and TF activates the downstream PI3K/Akt pathway. Tumor-derived over-expression of TF might play a role in chemotherapy resistance in glioblastoma, at lest in part, by activating PI3K/Akt-mediated survival and anti-apoptotic mechanism through the interaction of TF/FVII signaling.
基金funded by National Key Research and Development Program of China(No.2022YFC3501705)National Natural Science Foundation of China general projects(No.82274066)Shanghai Science and Technology Innovation Project(No.22S21901200).
文摘Gastric ulcer(GU)is a common digestive system disease.Acupuncture,as one of the external treatments of traditional Chinese medicine(TCM),has the characteristics of multi-target,multi-pathway and multi-level action in the treatment of GU.The relationship between meridian points and Zang-fu is an important part of the theory of TCM,which is crucial for the diagnosis and treatment of diseases.There is an external and internal link between acupoints and Zang-fu.The pathological reaction of Zang-fu can manifest as acupoint sensitization,while stimulation of acupoints can play a therapeutic role in the internal Zang-fu.Therefore,the acupoint has the functions of reflecting and treating diseases.This review explores the tender points on the body surface of patients with GU and the rules of acupoint selection.In addition,Zusanli(ST36),as one of the most used acupoints of the stomach meridian,was selected to show the mechanisms behind acupoint stimulation in the treatment of GU in greater detail,specifically in the well-studied model of the stress GU(SGU).Hence,the mechanisms of acupuncture at ST36 and points commonly used in combination with ST36 to treat SGU are discussed further.Treatment effects can be achieved through anti-inflammatory and antioxidant activities,gastric mucosal injury repair,and interaction with the brain-gut axis.In summary,this review provides evidence for a comprehensive understanding of the phenomena and mechanism of acupoint functions for GU.
基金supported by the National Natural Science Foundation of China(NSFC)(Nos.52171206 and52271209)Key Project of Hebei Natural Science Foundation(Nos.F2024201031 and E20202201030)+4 种基金Beijing-Tianjin-Hebei Collaborative Innovation Community Construction Project(No.21344301D)the Second Batch of Young Talent of Hebei Province(Nos.70280016160250 and 70280011808)Key Fund in Hebei Province Department of Education China(No.ZD2021014)the Central Government Guide Local Funding Projects for Scientific and Technological Development(Nos.216Z4404G and 206Z4402G)Interdisciplinary Research Program of Natural Science of Hebei University(No.DXK202107)。
文摘The lithium-oxygen battery(LOB)is a promising source of green energy due to its energy density.However,the development of this technology is limited by the insoluble discharge product it produces.In this work,a cathode material with a p-n heterostructure of polyaniline(PANI)/ZnS is prepared to trap visible light,utilizing a ZnS quantum dot(ZnS QD)network to form a large number of photogenerated electron–hole pairs,thus promoting the generation and decomposition of Li_(2)O_(2).The prepared PANI/ZnS has an ultra-low overpotential of 0.06 V under illumination.Furthermore,density functional theory theoretical calculation has demonstrated the ability of the heterostructures to adsorb oxygen-containing intermediates,which not only facilitates the growth of Li_(2)O_(2),but also reduces the reaction energy required to decompose Li_(2)O_(2).The present work provides a solution to the problem of insolubility of discharge products in photo-assisted LOB.
基金This study received financial support from the Jilin Province Health and Technology Capacity Enhancement Project(Project Number:222Lc132).
文摘Objective: This study investigates the auxiliary role of resting-state electroencephalography (EEG) in the clinical diagnosis of attention-deficit hyperactivity disorder (ADHD) using machine learning techniques. Methods: Resting-state EEG recordings were obtained from 57 children, comprising 28 typically developing children and 29 children diagnosed with ADHD. The EEG signal data from both groups were analyzed. To ensure analytical accuracy, artifacts and noise in the EEG signals were removed using the EEGLAB toolbox within the MATLAB environment. Following preprocessing, a comparative analysis was conducted using various ensemble learning algorithms, including AdaBoost, GBM, LightGBM, RF, XGB, and CatBoost. Model performance was systematically evaluated and optimized, validating the superior efficacy of ensemble learning approaches in identifying ADHD. Conclusion: Applying machine learning techniques to extract features from resting-state EEG signals enabled the development of effective ensemble learning models. Differential entropy and energy features across multiple frequency bands proved particularly valuable for these models. This approach significantly enhances the detection rate of ADHD in children, demonstrating high diagnostic efficacy and sensitivity, and providing a promising tool for clinical application.
基金Supported by 2024 Basic Research and Applied Basic Research Project in Neijiang City(2024 NJJCYJZZXR001)。
文摘[Objectives]To explore the effectiveness of integrating ideological and political education based on the Nightingale spirit into the clinical practice curriculum for nursing interns.[Methods]A total of 90 questionnaires were distributed and analyzed among nursing interns and patients to evaluate multiple dimensions,including the interns satisfaction with the internship,mastery of clinical knowledge,ideological and political literacy,professional ethics,and social responsibility.[Results]The overall internship satisfaction of nursing interns reached 98.9%,indicating that the course design and teaching model were widely recognized by students.Secondly,the interns demonstrated excellent mastery of clinical knowledge,with 94.0%of students stating they had fully grasped the clinical knowledge taught during the internship,an increase of 18.6%compared to the previous cohort.In terms of ideological and political literacy,98%of students had a better understanding and application of the Nightingale spirit,an increase of 16.2%compared to the previous cohort.Additionally,the sense of professional identity was significantly enhanced,with 95.8%of students showing a stronger sense of identity and responsibility towards the nursing profession,an increase of 14.0%compared to the previous cohort.Patients also gave high evaluations of the interns performance,with 98.8%expressing satisfaction with the students attitude and performance in nursing care.[Conclusions]The curriculum model based on the integration of Nightingale spirit has effectively improved the professional quality,ideological and political quality and professional identity of nursing interns,and has been highly recognized by patients.It not only provides strong support for the practice of combining ideological and political work with professional courses in nursing education,but also provides a reference for the optimization of clinical practice teaching mode of nursing interns in the future.
基金financial support from the following sources:the National Natural Science Foundation of China(NSFC)(grants 52171206,52271209)the Key Project of Hebei Natural Science Foundation(F2024201031,E2020201030)+1 种基金the Science Research Project of Hebei Education Department(JCZX2025019)the Interdisciplinary Research Program of Hebei University(DXK202401)。
文摘The stability and safety of aqueous aluminum metal batteries(AAMBs)have garnered an enormous amount of attention.However,severe corrosion during cycling and inefficient deposition behavior at the Al anode hinder their application.In this paper,CeCl_(3)was selected as a water inhibition additive to achieve active water confinement,which dramatically improved the corrosion behavior and prolonged the cycle life of the Al electrode.A combination of spectroscopic characterization and computational analysis showed that Cl^(-)breaks hydrogen bonds in the electrolyte and has higher adsorption energy on Al,thus inhibiting water-induced corrosion.Meanwhile,Ce^(3+)has a stronger affinity for Al than H_(2)O,thus promoting the formation of the surface protective layer.Cl^(-)in the modified electrolyte results in less hydration around Al^(3+).Due to the excellent water inhibition effect of CeCl_(3),the corrosion phenomenon of Al electrodes was significantly improved,and the dominant growth of the Al(111)crystal plane was achieved.Al//Prussian blue analogue(PBA)full cells with CeCl_(3)exhibit significantly improved voltage polarization(0.342 V),cycle life(550 cycles),discharge specific capacity(112 mAh g^(-1)),and selfdischarge behavior(87.97%).The Ce^(3+)in the additive is also able to be co-intercalated into the PBA with Al^(3+),improving the stability of the PBA.However,the conductivity reduction of this strategy at higher concentrations needs to be further addressed.Compared with organic electrolyte and molten salt systems,the cycle life of the aqueous electrolyte in this work still falls short.This modification method paves the way for further development of efficient AAMBs.
基金2024 Scientific Research Project of Jilin Provincial Administration of Traditional Chinese Medicine(Project No.:2024139)。
文摘Objective:To observe the clinical efficacy of the method of softening the liver,harmonizing the spleen,and unblocking the orifices in the treatment of Xiaoke Neizhang with Qi and Yin deficiency type.Methods:Seventy-two patients with Xiaoke Neizhang of qi and yin deficiency type were selected from March 2024 to May 2025 and divided into a treatment group and a control group with 36 cases each according to the random number table method.The treatment group was treated with the method of softening the liver,harmonizing the spleen,and unblocking the orifices,which included oral administration of Softening the Liver and Harmonizing the Spleen Pills combined with iontophoresis of Xuesaitong Injection.The control group was treated with oral administration of calcium dobesilate capsules.Both groups were treated for 2 courses,and multiple indicators such as visual acuity,fundus condition,and TCM syndrome scores were compared between the two groups.Results:The total effective rate of the treatment group was higher than that of the control group.There were significant differences in visual acuity,fundus condition,and TCM syndrome scores between and within the groups(P<0.05).Conclusion:The method of softening the liver,harmonizing the spleen,and unblocking the orifices has a definite clinical effect in the treatment of Xiaoke Neizhang with Qi and Yin deficiency type.It has a direct effect,is painless,easy for patients to accept,and has high clinical application value.
基金financially supported by the Natural Science Foundation of Jilin Province Science and Technology Department(No.20230101221JC)the National Natural Science Foundation of China(Nos.52173115,52073278,52203189)the Research Foundation for Advanced Talents of Xiamen University of Technology(Nos.5010423019,YKJ22052R)。
文摘It is well known that cationic polymers have excellent antimicrobial capacity accompanied with high biotoxicity,to reduce biotoxicity needs to decrease the number of cationic groups on polymers,which will influence antimicrobial activity.It is necessary to design a cationic polymer mimic natural antimicrobial peptide with excellent antibacterial activity and low toxicity to solve the above dilemma.Here,we designed and prepared a series of cationic poly(β-amino ester)s(PBAEs)with different cationic contents,and introducing hydrophobic alkyl chain to adjust the balance between antimicrobial activity and biotoxicity to obtain an ideal antimicrobial polymer.The optimum one of synthesized PBAE(hydrophilic cationic monomer:hydrophobic monomer=5:5)was screened by testing cytotoxicity and minimum inhibitory concentration(MIC),which can effectively kill S.aureus and E.coli with PBAE concentration of15μg/m L by a spread plate bacteriostatic method and dead and alive staining test.The way of PBAE killing bacterial was destroying the membrane like natural antimicrobial peptide observed by scanning electron microscopy(SEM).In addition,PBAE did not exhibit hemolysis and cytotoxicity.In particular,from the result of animal tests,the PBAE was able to promote healing of infected wounds from removing mature S.aureus and E.coli on the surface of infected wound.As a result,our work offers a viable approach for designing antimicrobial materials,highlighting the significant potential of PBAE polymers in the field of biomedical materials.
文摘Objective:Anillin(ANLN)is considered an oncogene in various cancers,but its effect on cervical cancer remains poorly understood.Hence,this study aimed to describe the action of ANLN on cervical cancer development and investigate the potential mechanism.Methods:Analysis of ANLN expression and its association with survival in carcinoma and endocervical adenocarcinoma(CESC)patients based on GEO and UALCAN databases.The tumor and adjacent normal tissues of 100 cervical cancer cases were harvested to detect the ANLN expression and explore its relationship with patient survival.Cell proliferation,apoptosis,migration,and invasion were measured by utilizing 5-ethynyl-2′-deoxyuridine(EdU)staining,Flow cytometry,and Transwell assay,respectively.ANLN andWnt expression were analyzed by RT-qPCR andWestern Blot.Results:ANLN was significantly elevated in tumor tissues,and cervical cancer cases with high ANLN expression exhibited poor survival and high dead proportion.Besides,ANLN induced cervical cancer cell proliferation,migration,and invasion and restrained cell apoptosis.In addition,ANLN promoted Wnt/β-catenin pathway activation.Furthermore,ANLN accelerated cell aggressive behaviors and suppressed cell apoptosis via activating the Wnt/β-catenin signaling in cervical cancer.Conclusion:ANLN was enhanced in cervical cancer tissues and related to poor prognosis.ANLN accelerated cervical cancer cell aggressive behaviors and suppressed cell apoptosis via activating theWnt/β-catenin pathway.
基金supported by the National Key R&D Program of China(Grant Nos.2022YFB3603403,2021YFB3600502)the National Natural Science Foundation of China(Grant Nos.62075040,62301150)+3 种基金the Southeast University Interdisciplinary Research Program for Young Scholars(2024FGC1007)the Start-up Research Fund of Southeast University(RF1028623164)the Nanjing Science and Technology Innovation Project for Returned Overseas Talent(4206002302)the Fundamental Research Funds for the Central Universities(2242024K40015).
文摘Benefiting from the widespread potential applications in the era of the Internet of Thing and metaverse,triboelectric and piezoelectric nanogenerators(TENG&PENG)have attracted considerably increasing attention.Their outstanding characteristics,such as self-powered ability,high output performance,integration compatibility,cost-effectiveness,simple configurations,and versatile operation modes,could effectively expand the lifetime of vastly distributed wearable,implantable,and environmental devices,eventually achieving self-sustainable,maintenance-free,and reliable systems.However,current triboelectric/piezoelectric based active(i.e.self-powered)sensors still encounter serious bottlenecks in continuous monitoring and multimodal applications due to their intrinsic limitations of monomodal kinetic response and discontinuous transient output.This work systematically summarizes and evaluates the recent research endeavors to address the above challenges,with detailed discussions on the challenge origins,designing strategies,device performance,and corresponding diverse applications.Finally,conclusions and outlook regarding the research gap in self-powered continuous multimodal monitoring systems are provided,proposing the necessity of future research development in this field.
基金supported by the National Natural Science Foundation of China(22274095 and 21974084)the Fundamental Research Funds for the Central Universities(GK202302004).
文摘Sonodynamic therapy(SDT)is garnering considerable attention as a promising treatment for deep-seated tumors because of its strong tissue penetration ability,non-invasiveness,and controllability.However,the SDT efficiency of traditional sonosensitizers including porphyrins and their derivatives are limited due to their poor water dissolubility,high aggregation,and low reactive oxygen species(ROS)production efficiency.Consequently,it is crucial to develop novel sonosensitizers with high yields of ROS,outstanding water solubility,and good biocompatibility.Herein,we constructed a new platform for SDT based on unimolecular porphyrin derivatives OPV-C_(3)-TPP.The probe OPV-C_(3)-TPP was synthesized by covalently linking conjugated oligomers(OPV)with 5,10,15,20-tetra(4-aminophenyl)porphyrin(TAPP).The introduction of OPV greatly improves the water solubility of the porphyrins and reduces the self-aggregation of the porphyrins.In addition,OPV-C_(3)-TPP has good intramolecular energy transfer efficiency,thus enhancing the yield of ROS.The experimental results show that OPV-C_(3)-TPP exhibits excellent ROS generation capacity under ultrasound(US)irradiation,which leads to apoptosis and necrosis of tumor cells.In vivo tumor growth is also significantly inhibited in the OPV-C_(3)-TPP t US group,exhibiting better SDT effects than TAPP.Therefore,the unimolecular OPV-C_(3)-TPP can be used as a potential sonosensitizer,providing a promising SDT for deep-tissue tumors.
基金National Key Research and Development Program of China(No.2022YFE0203600,China)National Natural Science Foundation of China(Nos.82341232,81925035)+3 种基金Department of Science and Technology of Guangdong Province(No.2021B0909050003)Chinese Academy of Sciences President's International Fellowship Initiative(No.2024VBB0004)the Scientific Innovation Group Project in Zhongshan(No.CXTD2022011)supported by grants from the Program of Shanghai Committee of Science and Technology,China(No.22S21902900)。
文摘The concurrence of primary sclerosing cholangitis(PSC)and inflammatory bowel disease(IBD)presents a therapeutic challenge,often necessitating liver transplantation in severe cases.Paeoniflorin(PAE),known for its immunomodulatory and anti-inflammatory properties but with very high-water solubility and low permeability,is formulated into a paeoniflorin/phospholipid complex microemulsion(PAE-ME)to enhance its delivery in this study.It demonstrated the PAE-ME's macrophage-regulating ability to repolarize the pro-inflammatory M1 subtype to the anti-inflammatory M2 type and reduce inflammatory cytokine release.In a PSC-IBD mouse model,PAE-ME alleviated the symptoms and regulated bile acid balance.Given the close connection and crosstalk between the liver and intestine,PAE-ME yielded a synergistic therapeutic effect on both the liver and intestinal lesions.These findings suggest a promising translational approach for complex comorbidities by acting on the liver-gut axis.
基金supported by the National Natural Science Foundation of China(Nos.52031005,52201224)the Natural Science Foundation of Shanghai(No.24ZR1438200)+1 种基金the Shanghai Academy of Spaceflight Technology Joint Research Fund(No.USCAST2023-19)the Equipment Development Depart-ment Huiyan Action.
文摘Shape memory alloys(SMAs)are smart materials with superelasticity originating from a reversible stressinduced martensitic transformation(MT)accompanied by a significant electrical resistance change.However,the stress-strain and resistance-stress relationships of typical NiTi wires are non-linear due to the stress plateau during the stress-induced MT.This limits the usage of these materials as pressure sensors.Herein,we propose a high-strength flexible sensor based on superelastic NiTi wires that achieves near-linear mechanical and electrical responses through a low-cost double-braided strategy.This microarchitectured strategy reduces or even eliminates stress plateau and it is demonstrated that the phase transformation of microfilaments can be controlled:regions with localized stress undergo the MT first,which is successively followed by the rest of the microfilament.This structure-dependent MT characteristic exhibits slim-hysteresis superelasticity and tunable low stiffness,and the braided wire shows improved flexibility.The double-braided NiTi microfilaments exhibit stable electrical properties and repeatability under approximately 600 MPa(8%strain)and can maintain stability over a wide temperature range(303-403 K).Moreover,a cross-grid flexible woven sensor array textile based on microfilaments is further developed to detect pressure distribution.This work provides insight into the design and application of SMAs in the field of flexible and functional fiber.
