Human adenovirus type 108(HAdV-108)has been detected in multiple countries,including China,and is associated with severe acute respiratory infection(ARI)in children,with reported fatalities.However,studies on HAdV-108...Human adenovirus type 108(HAdV-108)has been detected in multiple countries,including China,and is associated with severe acute respiratory infection(ARI)in children,with reported fatalities.However,studies on HAdV-108 remain limited.This study aimed to investigate the clinical and genetic characteristics of HAdV-108 in ARI children in China.From 2014 to 2024,6720 respiratory samples were collected from hospitalized children with ARI at ten hospitals across northern and southern China,of which 505(7.51%)tested positive for HAdV.The whole-genome and three major capsid protein genes were amplified and sequenced for bioinformatics analysis,which revealed that among 317 HAdV-isolated samples,21(6.62%)were identified as HAdV-108,ranking third after HAdV-114 and HAdV-7.Clinical analysis of HAdV-108-positive cases showed that the main manifestations were cough and fever.Seven children had gastrointestinal symptoms,and two children without underlying diseases were diagnosed with severe pneumonia.Phylogenetic analysis of wholegenome sequences revealed distinct predominant epidemic branches between domestic and international strains,with one strain obtained in this study forming an independent branch.Hexon protein exhibited the fastest evolution rate,lowest identity,and greatest amino acid variability,while fiber protein displayed the slowest evolution rate,highest identity,and greatest conservation and stability.Compared with the earliest reported HAdV-108 strain,three amino acid deletions were identified in the RGD loop region of penton base protein,resulting in potential structural change.Recombination analysis identified five distinct recombination patterns.In vitro experiments demonstrated that HAdV-108 had proliferation capacity comparable to other species C adenoviruses.In summary,HAdV-108 has persistently circulated in China,causing severe ARIs and concurrent gastrointestinal manifestations.Cluster3 was the predominant epidemic branch in China.HAdV-108 exhibited significant intratype genetic variation,with random and diverse recombination events.展开更多
Situated in the southwestern Pacific,the Tonga-Kermadec subduction zone is separated into two parts by the Louisvlle Ridge Seamount Chain(LRSC),i.e.,the Tanga subduction zone and the Kermadec subduction zone.Known for...Situated in the southwestern Pacific,the Tonga-Kermadec subduction zone is separated into two parts by the Louisvlle Ridge Seamount Chain(LRSC),i.e.,the Tanga subduction zone and the Kermadec subduction zone.Known for its vigorous volcanic activity,frequent large earthquakes,rapid plate subduction,and distinctive subducting plate morphology,this subduction zone provides valuable insights into its structures,dynamics,and associated geohazards.This study compiles geological and geophysical datasets in this region,including seismicity,focal mechanisms,seismic reflection and refraction profiles,and seismic tomography,to understand the relationship between lithospheric structures of the subduction system and associated seismicity-volcanic activities.Our analysis suggests that variations in overlying sediment thickness,subduction rate,and subduction angle significantly influence the lithospheric deformation processes within the Tonga-Kermadec subduction system.Furthermore,these factors contribute to the notable differences in seismicity and volcanism observed between the Tonga subduction zone and the Kermadec subduction zone.This study enhances our understanding of plate tectonics by providing insights into the interplay between subduction dynamics and lithospheric deformation,which are crucial for analyzing geological and geophysical behaviors in similar subduction environments.展开更多
基金supported by National Key Research and Development Program of China(2023YFC2306001)National Natural Science Foundation of China(No.32470141)+1 种基金the Beijing Research Center for Respiratory Infectious Diseases Project(BJRID2025-008)CAMS Innovation Fund for Medical Sciences(CIFMS,NO.2019-I2M-5–026,2022-I2M-CoV19-006).
文摘Human adenovirus type 108(HAdV-108)has been detected in multiple countries,including China,and is associated with severe acute respiratory infection(ARI)in children,with reported fatalities.However,studies on HAdV-108 remain limited.This study aimed to investigate the clinical and genetic characteristics of HAdV-108 in ARI children in China.From 2014 to 2024,6720 respiratory samples were collected from hospitalized children with ARI at ten hospitals across northern and southern China,of which 505(7.51%)tested positive for HAdV.The whole-genome and three major capsid protein genes were amplified and sequenced for bioinformatics analysis,which revealed that among 317 HAdV-isolated samples,21(6.62%)were identified as HAdV-108,ranking third after HAdV-114 and HAdV-7.Clinical analysis of HAdV-108-positive cases showed that the main manifestations were cough and fever.Seven children had gastrointestinal symptoms,and two children without underlying diseases were diagnosed with severe pneumonia.Phylogenetic analysis of wholegenome sequences revealed distinct predominant epidemic branches between domestic and international strains,with one strain obtained in this study forming an independent branch.Hexon protein exhibited the fastest evolution rate,lowest identity,and greatest amino acid variability,while fiber protein displayed the slowest evolution rate,highest identity,and greatest conservation and stability.Compared with the earliest reported HAdV-108 strain,three amino acid deletions were identified in the RGD loop region of penton base protein,resulting in potential structural change.Recombination analysis identified five distinct recombination patterns.In vitro experiments demonstrated that HAdV-108 had proliferation capacity comparable to other species C adenoviruses.In summary,HAdV-108 has persistently circulated in China,causing severe ARIs and concurrent gastrointestinal manifestations.Cluster3 was the predominant epidemic branch in China.HAdV-108 exhibited significant intratype genetic variation,with random and diverse recombination events.
基金supported by Special Projects in Universities’Key Fields of Guangdong Province(No.2023ZDZX3017)the 2022 Tertiary Education Scientific Research Project of Guangzhou Municipal Education Bureau(No.202234607)+1 种基金the Guangdong Basic and Applied Basic Research Foundation(No.2025A1515012983)the National Natural Science Foundation of China(Nos.52371059 and 52101358).
文摘Situated in the southwestern Pacific,the Tonga-Kermadec subduction zone is separated into two parts by the Louisvlle Ridge Seamount Chain(LRSC),i.e.,the Tanga subduction zone and the Kermadec subduction zone.Known for its vigorous volcanic activity,frequent large earthquakes,rapid plate subduction,and distinctive subducting plate morphology,this subduction zone provides valuable insights into its structures,dynamics,and associated geohazards.This study compiles geological and geophysical datasets in this region,including seismicity,focal mechanisms,seismic reflection and refraction profiles,and seismic tomography,to understand the relationship between lithospheric structures of the subduction system and associated seismicity-volcanic activities.Our analysis suggests that variations in overlying sediment thickness,subduction rate,and subduction angle significantly influence the lithospheric deformation processes within the Tonga-Kermadec subduction system.Furthermore,these factors contribute to the notable differences in seismicity and volcanism observed between the Tonga subduction zone and the Kermadec subduction zone.This study enhances our understanding of plate tectonics by providing insights into the interplay between subduction dynamics and lithospheric deformation,which are crucial for analyzing geological and geophysical behaviors in similar subduction environments.
