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精液生产中温度管理对猪精子质量的影响 被引量:7
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作者 李军伟 黄古月 +7 位作者 赵文明 朱家桥 鞠辉明 王帅彪 戴久丽 贾慧言 陈淑芳 刘宗平 《中国兽医学报》 CAS CSCD 北大核心 2023年第7期1513-1519,共7页
为研究精液生产中温度管理对猪精子质量的影响及机制,将采集后的猪精液样品等分成3份并分别于17℃(对照组)、22℃(公猪站实验室温度)和37℃(用于稀释液预温的水浴锅温度)下保存24 h。在0,6,18和24 h分别取样检测猪精子活率、活力、精子... 为研究精液生产中温度管理对猪精子质量的影响及机制,将采集后的猪精液样品等分成3份并分别于17℃(对照组)、22℃(公猪站实验室温度)和37℃(用于稀释液预温的水浴锅温度)下保存24 h。在0,6,18和24 h分别取样检测猪精子活率、活力、精子质膜和顶体膜完整性、细胞内ROS水平及精子凋亡率。在保存24 h后取样检测精子p-AMPKα、LKB-1、CaMKKβ、Bax、Bcl-2、Hsp70和Hsp90蛋白表达量。结果表明,与17℃相比,22℃下保存精子活率和活力在24 h后显著下降(P<0.05)。与17℃和22℃相比,37℃下保存精子各质量参数均显著下降(P<0.05)且出现质量损伤的时间更早,细胞内ROS水平显著升高(P<0.01),保存18 h后精子早期凋亡水平显著降低(P<0.05)而晚期凋亡水平显著升高(P<0.05)。与17℃和37℃相比,22℃保存24 h后精子p-AMPKα水平显著升高(P<0.05)。与17℃和22℃相比,37℃保存24 h后AMPK上游激酶LKB-1和CaMKKβ的表达量显著降低(P<0.05),凋亡蛋白Bcl-2、Bax和Caspase-3及热休克蛋白Hsp70表达量显著下降(P<0.05)。综合以上结果,精液生产过程中17~22℃下保存不会显著影响猪精子质量,精子通过LKB-1途径激活AMPK调节凋亡和热休克蛋白的表达来应答温度的升高。37℃下保存促进了精子早期凋亡向晚期凋亡的转化,破坏了精子的调节机制,不利于精子的存活。 展开更多
关键词 猪精液 温度管理 凋亡 AMPK 热休克蛋白
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Analysis of Plastic Effect and Clinical Application of Nasal Tip Plastic Surgery
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作者 LINJianxin GONGYonghao lijunwei 《外文科技期刊数据库(文摘版)医药卫生》 2022年第8期010-014,共5页
Objective: to explore the plastic effect and clinical application of nasal tip plastic surgery. Methods: a total of 78 patients were selected in this study, who underwent nasal tip plastic surgery in our hospital from... Objective: to explore the plastic effect and clinical application of nasal tip plastic surgery. Methods: a total of 78 patients were selected in this study, who underwent nasal tip plastic surgery in our hospital from January 2021 to January 2022. They were randomly assigned into two groups according to the odd-even series method. The experimental group and the conventional group were treated with artificial dermis and silica gel nasal augmentation, respectively, to compare the surgical effects. Results: 97.44% of the patients in the experimental group had effective nasal tip plastic surgery, while only 84.62% of the patients in the conventional group had effective nasal tip plastic surgery, which was statistically significant (P < 0.05). After plastic surgery, the nasal tip protrusion, nasal length and nasal tip height in the experimental group were significantly higher than those in the conventional group, and the statistical significance was established (P < 0.05). In the experimental group, 5.13% of the patients had complications after nasal tip plastic surgery, the total incidence rate was lower than 20.51% in the conventional group, and the statistical significance was established (P < 0.05). 97.44% of the patients in the experimental group were satisfied with the nasal tip plastic surgery, while only 82.05% of the patients in the conventional group were satisfied, which was statistically significant (P < 0.05). Conclusion: in the process of nasal tip plastic surgery, the overall application effect of artificial dermis is better than that of silica gel nasal augmentation, which is more worthy of promotion and application in clinic. 展开更多
关键词 nasal tip plastic surgery artificial dermis silicone prosthesis rhinoplasty
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