The present paper covers the synthesis and the characterization of ligand 2-decyl-3,6,9-tris(carboxymethyl)-3,6,9-triazaundecan-1,11-dioic acid,H_5L,and its Gd(Ⅲ) chelate. The protonation constants for H_5L(lg K H_...The present paper covers the synthesis and the characterization of ligand 2-decyl-3,6,9-tris(carboxymethyl)-3,6,9-triazaundecan-1,11-dioic acid,H_5L,and its Gd(Ⅲ) chelate. The protonation constants for H_5L(lg K H_ i =10.90,8.50,4.55,2.92,2.20) and the stability constant for GdL 2- (lg K _ GdL 2- =22.80) were determined by means of potentiometric titration. They are similar to the corresponding values of DTPA and Gd-DTPA,respectively. The results obtained show that the basicity of the ligand and the stability constant of its Gd(Ⅲ) chelate are not obviously altered after the introduction of a linear chain decyl group into the terminal acetic acid residue of DTPA. The Gd(Ⅲ) chelate may be a potential contrast agent with liver-specificity for magnetic resonance imaging(MRI).展开更多
Effects of Nd on the mechanical properties of die casting magnesium alloysAM50 are investigated. It is found that the ultimate tensile strength, yield strength and elongationof the AM50 alloy at elevated temperatures ...Effects of Nd on the mechanical properties of die casting magnesium alloysAM50 are investigated. It is found that the ultimate tensile strength, yield strength and elongationof the AM50 alloy at elevated temperatures up to 200 deg C increase with an addition of Nd.Generally, the most significant improvement in the tensile properties is attained at 100 deg C. Thepresence of Mg12Nd is proved by X-ray diffraction. Microstructural observations reveal that theaddition of Nd can lead to the dispersion-strengthening. Due to the presence of Mg12Nd, the slidingof grain boundaries is restrained and the strength of the alloy is enhanced. It can be seen from thetensile fractographs that the fracture surfaces exhibit the mixed fracture characteristics, andless brittle fracture is observed with increasing the temperature. Quasi-cleavage fracture isthought as the main fracture mode. TEM observations expose the formation of dislocation network,slip lines and dislocation pile-ups.展开更多
The formation and growth of thermal fatigue crack in low-chromium semi-steel were investigated by means of optical microscope and scanning electron microscope, and the function of RE in low-chromium semi-steel was ana...The formation and growth of thermal fatigue crack in low-chromium semi-steel were investigated by means of optical microscope and scanning electron microscope, and the function of RE in low-chromium semi-steel was analyzed. The results show that the thermal fatigue cracks are mainly generated at eutectic carbides, and the cracks not only grow and spread but also join each other. RE can improve the eutectic carbide′s morphology, inhibit the generation and propagation of thermal fatigue cracks, and therefore promote the activation energy for the crack′s propagation, which is especially more noticeable in case of the RE modification in combination with heat treatment. The mathematical model of the crack propagation is put forward.展开更多
To explore the expression of human clotting factor Ⅸ (hFⅨ) cDNA in vitro and the feasibility of gene therapy for hemophilia B mice mediated by recombinant lentiviral vector, a recombinant hFⅨ lentiviral vector driv...To explore the expression of human clotting factor Ⅸ (hFⅨ) cDNA in vitro and the feasibility of gene therapy for hemophilia B mice mediated by recombinant lentiviral vector, a recombinant hFⅨ lentiviral vector driven by ubiquitin-C promoter, FUXW, and by ABP liver specific promoter, FAXW, was constructed respectively. Recombinant lentivirus was harvested from 293T cells by calcium phosphate-mediated transient cotransfection of three plasmids (transgene vector, CMV腞8.2, VSV-G). hFⅨ expression was detected in supernatant of 293T, BHK and L-02 cells infected with FUXW virus, whereas higher expression of hFⅨ levels (630 ng/106 cells/48 h) was detected only in L-02 cells infected with FAXW virus. Serum hFⅨ antigen was detected in all hemophilia B mice treated with FAXW virus by tail vein injection, an efficiency level of hFⅨ was observed (45 ng/mL, approximately 1% of normal human levels), the expression lasted for more than 60 d. The results indicated that HIV-based lentiviral vectors offer a promising approach to the gene therapy of hemophilia B.展开更多
文摘The present paper covers the synthesis and the characterization of ligand 2-decyl-3,6,9-tris(carboxymethyl)-3,6,9-triazaundecan-1,11-dioic acid,H_5L,and its Gd(Ⅲ) chelate. The protonation constants for H_5L(lg K H_ i =10.90,8.50,4.55,2.92,2.20) and the stability constant for GdL 2- (lg K _ GdL 2- =22.80) were determined by means of potentiometric titration. They are similar to the corresponding values of DTPA and Gd-DTPA,respectively. The results obtained show that the basicity of the ligand and the stability constant of its Gd(Ⅲ) chelate are not obviously altered after the introduction of a linear chain decyl group into the terminal acetic acid residue of DTPA. The Gd(Ⅲ) chelate may be a potential contrast agent with liver-specificity for magnetic resonance imaging(MRI).
基金This project is supported by Provincial Natural Science Foundation of Liaoning (No.002015)
文摘Effects of Nd on the mechanical properties of die casting magnesium alloysAM50 are investigated. It is found that the ultimate tensile strength, yield strength and elongationof the AM50 alloy at elevated temperatures up to 200 deg C increase with an addition of Nd.Generally, the most significant improvement in the tensile properties is attained at 100 deg C. Thepresence of Mg12Nd is proved by X-ray diffraction. Microstructural observations reveal that theaddition of Nd can lead to the dispersion-strengthening. Due to the presence of Mg12Nd, the slidingof grain boundaries is restrained and the strength of the alloy is enhanced. It can be seen from thetensile fractographs that the fracture surfaces exhibit the mixed fracture characteristics, andless brittle fracture is observed with increasing the temperature. Quasi-cleavage fracture isthought as the main fracture mode. TEM observations expose the formation of dislocation network,slip lines and dislocation pile-ups.
文摘The formation and growth of thermal fatigue crack in low-chromium semi-steel were investigated by means of optical microscope and scanning electron microscope, and the function of RE in low-chromium semi-steel was analyzed. The results show that the thermal fatigue cracks are mainly generated at eutectic carbides, and the cracks not only grow and spread but also join each other. RE can improve the eutectic carbide′s morphology, inhibit the generation and propagation of thermal fatigue cracks, and therefore promote the activation energy for the crack′s propagation, which is especially more noticeable in case of the RE modification in combination with heat treatment. The mathematical model of the crack propagation is put forward.
文摘To explore the expression of human clotting factor Ⅸ (hFⅨ) cDNA in vitro and the feasibility of gene therapy for hemophilia B mice mediated by recombinant lentiviral vector, a recombinant hFⅨ lentiviral vector driven by ubiquitin-C promoter, FUXW, and by ABP liver specific promoter, FAXW, was constructed respectively. Recombinant lentivirus was harvested from 293T cells by calcium phosphate-mediated transient cotransfection of three plasmids (transgene vector, CMV腞8.2, VSV-G). hFⅨ expression was detected in supernatant of 293T, BHK and L-02 cells infected with FUXW virus, whereas higher expression of hFⅨ levels (630 ng/106 cells/48 h) was detected only in L-02 cells infected with FAXW virus. Serum hFⅨ antigen was detected in all hemophilia B mice treated with FAXW virus by tail vein injection, an efficiency level of hFⅨ was observed (45 ng/mL, approximately 1% of normal human levels), the expression lasted for more than 60 d. The results indicated that HIV-based lentiviral vectors offer a promising approach to the gene therapy of hemophilia B.
