Tripterygium hypoglaucum(Levl.)Hutch,a traditional Chinese medicinal herb with a long history of use,is widely distributed in China.One of its main active components,celastrol,has great potential to be developed into ...Tripterygium hypoglaucum(Levl.)Hutch,a traditional Chinese medicinal herb with a long history of use,is widely distributed in China.One of its main active components,celastrol,has great potential to be developed into anti-cancer and anti-obesity drugs.Although it exhibits strong pharmacological activities,there is a lack of sustainable sources of celastrol and its derivatives,making it crucial to develop novel sources of these drugs through synthetic biology.The key step in the biosynthesis of celastrol is considered to be the cyclization of 2,3-oxidosqualene into friedelin under the catalysis of 2,3-oxidosqualene cyclases.Friedelin was speculated to be oxidized into celastrol by cytochrome P450 oxidases(CYP450s).Here,we reported a cytochrome P450 ThCYP712K1 from Tripterygium hypoglaucum(Levl.)Hutch that catalyzed the oxidation of friedelin into polpuonic acid when heterologously expressed in yeast.Through substrate supplementation and in vitro enzyme analysis,ThCYP712K1 was further proven to catalyze the oxidation of friedelin at the C-29 position to produce polpunonic acid,which is considered a vital step in the biosynthesis of celastrol,and will lay a foundation for further analysis of its biosynthetic pathway.展开更多
A new tool force model to be presented is based upon process geometry and thecharacteristics of the force system, in which the forces acting on the tool rake face, the cuttingedge rounding and the clearance face have ...A new tool force model to be presented is based upon process geometry and thecharacteristics of the force system, in which the forces acting on the tool rake face, the cuttingedge rounding and the clearance face have been considered, and the size effect is accountable forthe new model. It is desired that the model can be well applicable to conventional diamond turningand the model may be employed as a tool in the design of diamond tools. This approach is quitedifferent from traditional investigations primarily based on empirical studies. As the depth of cutbecomes the same order as the rounded cutting edge radius, sliding along the clearance face due toelastic recovery of workpiece material and plowing due to the rounded cutting edge may becomeimportant in micro-machining, the forces acting on the cutting edge rounding and the clearance facecan not be neglected. For this reason, it is very important to understand the influence of someparameters on tool forces and develop a model of the relationship between them.展开更多
Objective: to carry out stratified nursing care for uremic patients undergoing hemodialysis, and analyze the effect of nursing care on nutritional status and quality of life of patients. Methods: a total of 80 patient...Objective: to carry out stratified nursing care for uremic patients undergoing hemodialysis, and analyze the effect of nursing care on nutritional status and quality of life of patients. Methods: a total of 80 patients admitted in this study were uremic patients treated with hemodialysis in Tongji hospital from May 2020 to May 2021. All patients were equally divided into two groups according to the ball-touching grouping method: observation group (40 cases) and control group (40 cases). Patients in the control group received routine care during the treatment, while patients in the observation group received stratified care during the treatment. After completing the nursing, the data of the two groups of patients were collected, including the patients' nutritional status, emotional status, quality of life and patients' compliance with the treatment. Results: the nutritional status of the patients in the observation group was significantly higher than that of the patients in the control group (P < 0.05). The scores of emotional state indexes in the observation group were significantly lower than those in the control group (P < 0.05). The quality of life index of the observation group was significantly higher than that of the control group (P < 0.05). The total treatment compliance of the observation group was 97.5%, which was higher than 82.5% of the control group (P < 0.05). Conclusion: layered nursing model can help uremic patients to improve their quality of life, and is worthy of promotion.展开更多
Objective: to explore the clinical application value of hemiplegic rehabilitation decoction in the rehabilitation of acute cerebral infarction. Methods: 86 patients with acute cerebral infarction were randomly divided...Objective: to explore the clinical application value of hemiplegic rehabilitation decoction in the rehabilitation of acute cerebral infarction. Methods: 86 patients with acute cerebral infarction were randomly divided into the same number of control group and study group (n 43). They were treated with conventional western medicine and additional hemiplegic rehabilitation decoction, respectively. The therapeutic conditions of the two groups were observed and compared. Results: compared with the control group, the effective rate of the study group was higher (P < 0.05). Before and after treatment, the increase of BI score, NIHSS score, TCM syndrome score and the level of inflammatory factors in the study group decreased significantly (P < 0.05). Conclusion: the application of hemiplegic rehabilitation decoction during the rehabilitation treatment of patients with acute cerebral infarction can achieve ideal effect, improve the neurological function defects of patients and enhance their daily living ability, which is worth promoting and applying.展开更多
Objective: to explore the application value of fiberoptic bronchoscope and alveolar lavage in children with refractory pneumonia. Methods: the clinical study selected 80 cases of children, as a clinical analysis objec...Objective: to explore the application value of fiberoptic bronchoscope and alveolar lavage in children with refractory pneumonia. Methods: the clinical study selected 80 cases of children, as a clinical analysis object, the study starting time is in August 2021, the clinical analysis data collection, the research plan, until August 2022, the study selected case, as a group standard, the group to nursing path intervention, control group to basic intervention, the number of children in each group of 40 cases, fiber bronchoscope alveolar lavage, analysis of lung function, satisfaction, compliance, improvement time, nursing quality. Results: the group family satisfaction is higher, compliance, nursing quality is better than the control group, children with disease improvement time, lung function improvement effect is better than the control group, P <0.05. Conclusion: clinical intervention during refractory pneumonia symptoms fiber bronchoscope alveolar lavage nursing pathway comprehensive intervention, has high clinical application value, can significantly improve the treatment effect, increase the patient care service compliance, shorten the disease rehabilitation time, can use for primary medical institutions, worthy of widely recommended clinically.展开更多
The Party organization in higher vocational colleges is the basis for the Party to carry out all the work in higher vocational colleges and the Partys combat effectiveness. Its construction is the inherent requirement...The Party organization in higher vocational colleges is the basis for the Party to carry out all the work in higher vocational colleges and the Partys combat effectiveness. Its construction is the inherent requirement to cultivate morality and cultivate people in higher vocational colleges, the inevitable choice to strengthen the Party organization construction in higher vocational colleges, and the objective need to practice people-centered thinking. Party organizations in higher vocational colleges should keep up with the pace of the new era and explore the path of construction. Firstly, they should focus on improving the leading power of party organizations based on learning;Secondly, we should take service as the goal and strive to improve the execution of the party organization;Thirdly, we should take innovation as the driving force and strive to enhance the combat effectiveness of the Party organization.展开更多
"Micro-education" is a brand-new educational concept based on the arrival of micro-era. This paper explores the content, carrier, means and goal of ideological and political education for higher vocational s..."Micro-education" is a brand-new educational concept based on the arrival of micro-era. This paper explores the content, carrier, means and goal of ideological and political education for higher vocational students from the perspective of micro-media, analyzes the opportunities and challenges facing the development of "micro-education" for higher vocational students, and puts forward the development path of "micro-education" for higher vocational students.展开更多
基金supported by the National Key R&D Program of China(No.2020YFA0908000)the National Natural Science Foundation of China(Nos.81773830 and 81973418)the Key Project at Central Government Level:the ability establishment of sustainable use for valuable Chinese medicine resources(No.2060302-1806-03),and the National Program for Special Support of Eminent Professionals.
文摘Tripterygium hypoglaucum(Levl.)Hutch,a traditional Chinese medicinal herb with a long history of use,is widely distributed in China.One of its main active components,celastrol,has great potential to be developed into anti-cancer and anti-obesity drugs.Although it exhibits strong pharmacological activities,there is a lack of sustainable sources of celastrol and its derivatives,making it crucial to develop novel sources of these drugs through synthetic biology.The key step in the biosynthesis of celastrol is considered to be the cyclization of 2,3-oxidosqualene into friedelin under the catalysis of 2,3-oxidosqualene cyclases.Friedelin was speculated to be oxidized into celastrol by cytochrome P450 oxidases(CYP450s).Here,we reported a cytochrome P450 ThCYP712K1 from Tripterygium hypoglaucum(Levl.)Hutch that catalyzed the oxidation of friedelin into polpuonic acid when heterologously expressed in yeast.Through substrate supplementation and in vitro enzyme analysis,ThCYP712K1 was further proven to catalyze the oxidation of friedelin at the C-29 position to produce polpunonic acid,which is considered a vital step in the biosynthesis of celastrol,and will lay a foundation for further analysis of its biosynthetic pathway.
基金This project is supported by National Natural Science Foundation of China (No.50175022)National Aerospace Support Foundation of China(No.0223HIT07).
文摘A new tool force model to be presented is based upon process geometry and thecharacteristics of the force system, in which the forces acting on the tool rake face, the cuttingedge rounding and the clearance face have been considered, and the size effect is accountable forthe new model. It is desired that the model can be well applicable to conventional diamond turningand the model may be employed as a tool in the design of diamond tools. This approach is quitedifferent from traditional investigations primarily based on empirical studies. As the depth of cutbecomes the same order as the rounded cutting edge radius, sliding along the clearance face due toelastic recovery of workpiece material and plowing due to the rounded cutting edge may becomeimportant in micro-machining, the forces acting on the cutting edge rounding and the clearance facecan not be neglected. For this reason, it is very important to understand the influence of someparameters on tool forces and develop a model of the relationship between them.
文摘Objective: to carry out stratified nursing care for uremic patients undergoing hemodialysis, and analyze the effect of nursing care on nutritional status and quality of life of patients. Methods: a total of 80 patients admitted in this study were uremic patients treated with hemodialysis in Tongji hospital from May 2020 to May 2021. All patients were equally divided into two groups according to the ball-touching grouping method: observation group (40 cases) and control group (40 cases). Patients in the control group received routine care during the treatment, while patients in the observation group received stratified care during the treatment. After completing the nursing, the data of the two groups of patients were collected, including the patients' nutritional status, emotional status, quality of life and patients' compliance with the treatment. Results: the nutritional status of the patients in the observation group was significantly higher than that of the patients in the control group (P < 0.05). The scores of emotional state indexes in the observation group were significantly lower than those in the control group (P < 0.05). The quality of life index of the observation group was significantly higher than that of the control group (P < 0.05). The total treatment compliance of the observation group was 97.5%, which was higher than 82.5% of the control group (P < 0.05). Conclusion: layered nursing model can help uremic patients to improve their quality of life, and is worthy of promotion.
文摘Objective: to explore the clinical application value of hemiplegic rehabilitation decoction in the rehabilitation of acute cerebral infarction. Methods: 86 patients with acute cerebral infarction were randomly divided into the same number of control group and study group (n 43). They were treated with conventional western medicine and additional hemiplegic rehabilitation decoction, respectively. The therapeutic conditions of the two groups were observed and compared. Results: compared with the control group, the effective rate of the study group was higher (P < 0.05). Before and after treatment, the increase of BI score, NIHSS score, TCM syndrome score and the level of inflammatory factors in the study group decreased significantly (P < 0.05). Conclusion: the application of hemiplegic rehabilitation decoction during the rehabilitation treatment of patients with acute cerebral infarction can achieve ideal effect, improve the neurological function defects of patients and enhance their daily living ability, which is worth promoting and applying.
文摘Objective: to explore the application value of fiberoptic bronchoscope and alveolar lavage in children with refractory pneumonia. Methods: the clinical study selected 80 cases of children, as a clinical analysis object, the study starting time is in August 2021, the clinical analysis data collection, the research plan, until August 2022, the study selected case, as a group standard, the group to nursing path intervention, control group to basic intervention, the number of children in each group of 40 cases, fiber bronchoscope alveolar lavage, analysis of lung function, satisfaction, compliance, improvement time, nursing quality. Results: the group family satisfaction is higher, compliance, nursing quality is better than the control group, children with disease improvement time, lung function improvement effect is better than the control group, P <0.05. Conclusion: clinical intervention during refractory pneumonia symptoms fiber bronchoscope alveolar lavage nursing pathway comprehensive intervention, has high clinical application value, can significantly improve the treatment effect, increase the patient care service compliance, shorten the disease rehabilitation time, can use for primary medical institutions, worthy of widely recommended clinically.
文摘The Party organization in higher vocational colleges is the basis for the Party to carry out all the work in higher vocational colleges and the Partys combat effectiveness. Its construction is the inherent requirement to cultivate morality and cultivate people in higher vocational colleges, the inevitable choice to strengthen the Party organization construction in higher vocational colleges, and the objective need to practice people-centered thinking. Party organizations in higher vocational colleges should keep up with the pace of the new era and explore the path of construction. Firstly, they should focus on improving the leading power of party organizations based on learning;Secondly, we should take service as the goal and strive to improve the execution of the party organization;Thirdly, we should take innovation as the driving force and strive to enhance the combat effectiveness of the Party organization.
文摘"Micro-education" is a brand-new educational concept based on the arrival of micro-era. This paper explores the content, carrier, means and goal of ideological and political education for higher vocational students from the perspective of micro-media, analyzes the opportunities and challenges facing the development of "micro-education" for higher vocational students, and puts forward the development path of "micro-education" for higher vocational students.
