Phytochemical investigation of Lagotis yunnanensis led to the isolation and identification of a new iridoid glucoside 1, named as 10-O-(3,4-dimethoxy-(E)-cinnamoyl)aucubin. Its structure was elucidated by spectroscopi...Phytochemical investigation of Lagotis yunnanensis led to the isolation and identification of a new iridoid glucoside 1, named as 10-O-(3,4-dimethoxy-(E)-cinnamoyl)aucubin. Its structure was elucidated by spectroscopie methods.展开更多
Two new diacetylated flavonol glycosides, kampferol-3-O--3? 6?diacetylglucopyrano- side and quercetin-3-O--3? 6?diacetylglucopyranoside were isolated from knoxia corymbosa. Their structures were elucidated by spectro...Two new diacetylated flavonol glycosides, kampferol-3-O--3? 6?diacetylglucopyrano- side and quercetin-3-O--3? 6?diacetylglucopyranoside were isolated from knoxia corymbosa. Their structures were elucidated by spectroscopic evidents.展开更多
A new phytosterone named passionsterone 1 has been isolated from the roots of Passiflora wilsonii, along with the known compound 24R-ethyl-5 α-cholestane-3 β ,6 a -diol 2. The structure of passionsterone 1 was eluci...A new phytosterone named passionsterone 1 has been isolated from the roots of Passiflora wilsonii, along with the known compound 24R-ethyl-5 α-cholestane-3 β ,6 a -diol 2. The structure of passionsterone 1 was elucidated as 24R-ethyl-5 α -cholestane-3 β,6 α-diol-23-one by spectroscopic means.展开更多
OBJECTIVE To study the treatment effect of Gastrodia elata and Uncaria rhynchophylla water extract(GUW)against cerebral ischemia,and the metabolomic profile of cerebrospinal fluid in GUW treated cerebral ischemia in r...OBJECTIVE To study the treatment effect of Gastrodia elata and Uncaria rhynchophylla water extract(GUW)against cerebral ischemia,and the metabolomic profile of cerebrospinal fluid in GUW treated cerebral ischemia in rat model.METHODS Middle cerebral artery occlusion was performed in male SD rat.GUW or control(PBS)was administered orally for 7d post-operationally.At day 7,the brain of the rat was harvested.The infarct volume of the brain was measured after TTC staining.The brain was subjected to H&E and cresyl violet staining in histological analysis.The cerebrospinal fluid(CSF)of another set of animals was aspirated.The CSF samples were labeled with 13 C-dansyl and 12C-dansyl chloride.The reaction mixture was put into liquid chromatography Fourier-transform ion cyclotron resonance mass spectrometry for analysis of the CSF metabolome.Multivariate statistical analysis will be carried out to determine the differences of metabolomic profile in CSF of GUW versus control group.RESULTS After GUW treatment,the neurological deficit score was significantly improved from day 3 to 7(P<0.001).GUW treated group significantly reduced 55.7%infarct volume on MCAO rats in TTC stained brain slices(P<0.001)and preserved brain histological structure observed in H&E and cresyl violet staining.On the other hand,according to various statistical analyses to compare CSF metabolite in the GUW and control group,volcano plot analysis showed 48 metabolites(P<0.001);OPLS-DA analysis showed 129 metabolites(VIP score >2.0)and 45 metabolites in ROC curve analysis.Among identified metabolites,L-aspartic acid(60%)and glycine(62%)decreased in GUW while threoninyl-alanine increased by 1.6 fold significantly.CONCLUSION GUW ameliorated cerebral ischemic injury,and the metabolomic approach may be a potential means to find biomarker of the Chinese medicine treatment on cerebral ischemia.展开更多
Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabi...Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabinosyl-3-O-β-D-6'-acetylglucopyranoside and kaempferol -7-O-α-L-arabinosyl-3-O-β-D-3',6'-diacetylglucopyranoside respectively.展开更多
The so-called enterprise internal control work is in the daily operation of the enterprise in such a process, by the inside and outside the use of the relevant legal means and rules and regulations of the enterprise i...The so-called enterprise internal control work is in the daily operation of the enterprise in such a process, by the inside and outside the use of the relevant legal means and rules and regulations of the enterprise internal self-restraint and control of such a process. The evaluation of the effectiveness of internal control is also in the daily operation of the enterprise, the special supervision and management of the enterprise uses the effect of self-management. Of course, an excellent enterprise must have a certain sense of internal control to make the corresponding internal control evaluation system constructed successfully. And only with a standardized evaluation system can enterprises can run and develop with higher quality and long-term time. This paper analyzes the construction mode of the internal control effectiveness evaluation system, and provides a certain scientific reference and theoretical basis for the construction of the effectiveness evaluation system of the internal control work.展开更多
Abstract: Schisandra rubriflora Rehd. et Wils. is a traditional Chinese medicine. To search for new and bioactive components from traditional Chinese medicines and provide scientific evidence for taxonomy, the chemica...Abstract: Schisandra rubriflora Rehd. et Wils. is a traditional Chinese medicine. To search for new and bioactive components from traditional Chinese medicines and provide scientific evidence for taxonomy, the chemical constituents of the plant were investigated by various column chromatography methods (silica gel, Sephadex LH-20, and RP-18). From the aerial parts of S. rubriflora, three new megastigmane glycosides, namely (3S, 5R, 6S, 9R)-megastigmane-3, 9-diol 3-O-[α-L-arabionfuranosyl-(1→6)-β-D-glucopyranoside] (1), 7-megastigmene-3-ol-9-one 3-O-[α-L-arabionfuranosyl-(1→6)-β-D-glucopyranoside] (2), and megastigmane-3α, 4β, 9σ-triol 3-O-β-D-glucopyranoside (3), along with 14 known compounds, were isolated. The structures of the new compounds were elucidated by a combination of spectroscopic and chemical methods.展开更多
文摘Phytochemical investigation of Lagotis yunnanensis led to the isolation and identification of a new iridoid glucoside 1, named as 10-O-(3,4-dimethoxy-(E)-cinnamoyl)aucubin. Its structure was elucidated by spectroscopie methods.
文摘Two new diacetylated flavonol glycosides, kampferol-3-O--3? 6?diacetylglucopyrano- side and quercetin-3-O--3? 6?diacetylglucopyranoside were isolated from knoxia corymbosa. Their structures were elucidated by spectroscopic evidents.
文摘A new phytosterone named passionsterone 1 has been isolated from the roots of Passiflora wilsonii, along with the known compound 24R-ethyl-5 α-cholestane-3 β ,6 a -diol 2. The structure of passionsterone 1 was elucidated as 24R-ethyl-5 α -cholestane-3 β,6 α-diol-23-one by spectroscopic means.
基金The project supported by the Health and Medical Research Fund,Hong Kong(11120381)
文摘OBJECTIVE To study the treatment effect of Gastrodia elata and Uncaria rhynchophylla water extract(GUW)against cerebral ischemia,and the metabolomic profile of cerebrospinal fluid in GUW treated cerebral ischemia in rat model.METHODS Middle cerebral artery occlusion was performed in male SD rat.GUW or control(PBS)was administered orally for 7d post-operationally.At day 7,the brain of the rat was harvested.The infarct volume of the brain was measured after TTC staining.The brain was subjected to H&E and cresyl violet staining in histological analysis.The cerebrospinal fluid(CSF)of another set of animals was aspirated.The CSF samples were labeled with 13 C-dansyl and 12C-dansyl chloride.The reaction mixture was put into liquid chromatography Fourier-transform ion cyclotron resonance mass spectrometry for analysis of the CSF metabolome.Multivariate statistical analysis will be carried out to determine the differences of metabolomic profile in CSF of GUW versus control group.RESULTS After GUW treatment,the neurological deficit score was significantly improved from day 3 to 7(P<0.001).GUW treated group significantly reduced 55.7%infarct volume on MCAO rats in TTC stained brain slices(P<0.001)and preserved brain histological structure observed in H&E and cresyl violet staining.On the other hand,according to various statistical analyses to compare CSF metabolite in the GUW and control group,volcano plot analysis showed 48 metabolites(P<0.001);OPLS-DA analysis showed 129 metabolites(VIP score >2.0)and 45 metabolites in ROC curve analysis.Among identified metabolites,L-aspartic acid(60%)and glycine(62%)decreased in GUW while threoninyl-alanine increased by 1.6 fold significantly.CONCLUSION GUW ameliorated cerebral ischemic injury,and the metabolomic approach may be a potential means to find biomarker of the Chinese medicine treatment on cerebral ischemia.
文摘Two new flavonol glycosides (1 and 2) together with two known flavonoides (3 and 4), were isolated from the whole plant of Knoxia corymbosa willd. The structures of 1 and 2 were elucidated as kaempferol-7-O-α-L-arabinosyl-3-O-β-D-6'-acetylglucopyranoside and kaempferol -7-O-α-L-arabinosyl-3-O-β-D-3',6'-diacetylglucopyranoside respectively.
文摘The so-called enterprise internal control work is in the daily operation of the enterprise in such a process, by the inside and outside the use of the relevant legal means and rules and regulations of the enterprise internal self-restraint and control of such a process. The evaluation of the effectiveness of internal control is also in the daily operation of the enterprise, the special supervision and management of the enterprise uses the effect of self-management. Of course, an excellent enterprise must have a certain sense of internal control to make the corresponding internal control evaluation system constructed successfully. And only with a standardized evaluation system can enterprises can run and develop with higher quality and long-term time. This paper analyzes the construction mode of the internal control effectiveness evaluation system, and provides a certain scientific reference and theoretical basis for the construction of the effectiveness evaluation system of the internal control work.
文摘Abstract: Schisandra rubriflora Rehd. et Wils. is a traditional Chinese medicine. To search for new and bioactive components from traditional Chinese medicines and provide scientific evidence for taxonomy, the chemical constituents of the plant were investigated by various column chromatography methods (silica gel, Sephadex LH-20, and RP-18). From the aerial parts of S. rubriflora, three new megastigmane glycosides, namely (3S, 5R, 6S, 9R)-megastigmane-3, 9-diol 3-O-[α-L-arabionfuranosyl-(1→6)-β-D-glucopyranoside] (1), 7-megastigmene-3-ol-9-one 3-O-[α-L-arabionfuranosyl-(1→6)-β-D-glucopyranoside] (2), and megastigmane-3α, 4β, 9σ-triol 3-O-β-D-glucopyranoside (3), along with 14 known compounds, were isolated. The structures of the new compounds were elucidated by a combination of spectroscopic and chemical methods.
