Objective: To investigate the effect of 5-aminolevulinic(ALA)-photodynamic therapy(PDT) on the expressions of MMP-9, MMP-13 and TIMP-1 of hypertrophic scar model in rabbit ears, and analyze the possible therapeutic me...Objective: To investigate the effect of 5-aminolevulinic(ALA)-photodynamic therapy(PDT) on the expressions of MMP-9, MMP-13 and TIMP-1 of hypertrophic scar model in rabbit ears, and analyze the possible therapeutic mechanisms of ALA-PDT treatment to hypertrophic scars of rabbit ears. Methods: The experimental animals were randomly divided into normal control, negative control, high concentration of ALA-PDT, low concentration of ALA-PDT and PDT groups. The latter three groups received ALA-PDT treatment or PDT treatment once a week for 3 weeks. The specimens of the rabbits were collected respectively 1, 2 and 3 months after treatment to be used for RT-PCR and Western-blot test. Results: 1, 2 and 3 months after PDT treatment, the expressions of MMP-9 and MMP-13(including mRNA and protein) in hypertrophic scar tissues of three treatment groups were significantly higher than those of the negative control group(P<0.01), and the expression of TIMP-1 mRNA and protein of three treatment groups were significantly lower than that of the negative control group(P<0.01). There were also significant differences between high-concentration ALA-PDT treatment group and the low one(P<0.05). Conclusion: ALA-PDT is effective in treating hypertrophic scars of rabbit ears, and its possible therapeutic mechanisms are that ALA-PDT treatment generates oxidation activation effect to activate the activity of MMPs and induces the photoaging of fibroblasts of hypertrophic scar tissues of rabbit ears to inhibit the activity of TIMPs, which causes the up-regulation of MMPs and the down-regulation of TIMPs. Because of this, the degradation of collagen and ECM is accelerated and the formation of scars is suppressed.展开更多
We collect seismic moment tensors of the earthquakes occurring from 1900 to 2013 in and around the Chinese mainland and summarize the surface ruptures and displacements of 70 earthquakes with M S≥7. 0. We divide thes...We collect seismic moment tensors of the earthquakes occurring from 1900 to 2013 in and around the Chinese mainland and summarize the surface ruptures and displacements of 70 earthquakes with M S≥7. 0. We divide these large earthquakes into three types. Type A contains earthquakes with surface ruptures and displacements. Type B is earthquakes without displacements and Type C is those without any of this data. We simulate a triangular distribution of displacements for Type B and C. Then,we segment these large earthquakes by using their displacements and surface ruptures. Finally,kinematic models are determined from earthquake data and Bicubic Bessel spline functions. The results show that,first of all,the reasonability and spatial consistency of defined models are advanced.Strain rates have better continuity and are comparable with geologic and geodetic results in Himalaya thrust fault zones. The strain rates decrease in the Tarim basin and the Altun Tagh fault zones because of their low seismicity. The direction of compressional deformation in Gobi-Altay is changed from SE to NE and its extensional direction is changed from NE to NW. The extensional deformation in the Ordos block is diminished obviously. Secondly,earthquakes account for 30- 50% of expected motion of India relative to Eurasia determined from the NUVEL-1A model,with a missing component of 20 mm / a which may contain aseismic deformation such as fault creep and folds,the missing parts of earthquake data and elastic strain energy released by potential earthquakes.展开更多
Since 2001, there have occurred in succession the 2001 Kunlun Mountains M S8. 1earthquake,the 2008 Wenchuan M S8. 0 earthquake,the 2010 Yushu M S7. 1 earthquake and the 2012 Lushan M S7. 0 earthquake in the periphery ...Since 2001, there have occurred in succession the 2001 Kunlun Mountains M S8. 1earthquake,the 2008 Wenchuan M S8. 0 earthquake,the 2010 Yushu M S7. 1 earthquake and the 2012 Lushan M S7. 0 earthquake in the periphery of the Bayan Har block. By comparison of the characteristics of seismic strain release variations before and after the Kunlun Mountains M S8. 1 earthquake in the same time length in the geodynamical related regions,we found that the seismic strain release was obviously enhanced after the earthquake in the Longmenshan area,Batang area,and the NS-trending valleys at the west of the Hot Spring Basin. The Wenchuan earthquake occurred in the first area,and the Yushu earthquake is related to the second area. After the earthquake rupture occurred on the East Kunlun fault zone on the northern boundary of the Bayan Har Block,crustal materials on the south side of the fault zone migrated to the southeast,leading to a concentration of tectonic deformation in the Longmenshan thrust belt, e ventually rupturing on the Longmenshan thrust belt. This earthquake case illustrates that seismicity enhancement zones are possibly prone to long-term destructive earthquakes. After the M S7. 3 earthquake in Yutian,Xinjiang on February 12,2014,earthquake frequency and seismic strain release markedly increased in the junction area between the eastern Qilian Mountain tectonic belt and the Altun Tagh fault zone,where more attention should be paid to the long-term seismic risk.展开更多
基金Supported by the National Natural Science Foundation of China(30901298)
文摘Objective: To investigate the effect of 5-aminolevulinic(ALA)-photodynamic therapy(PDT) on the expressions of MMP-9, MMP-13 and TIMP-1 of hypertrophic scar model in rabbit ears, and analyze the possible therapeutic mechanisms of ALA-PDT treatment to hypertrophic scars of rabbit ears. Methods: The experimental animals were randomly divided into normal control, negative control, high concentration of ALA-PDT, low concentration of ALA-PDT and PDT groups. The latter three groups received ALA-PDT treatment or PDT treatment once a week for 3 weeks. The specimens of the rabbits were collected respectively 1, 2 and 3 months after treatment to be used for RT-PCR and Western-blot test. Results: 1, 2 and 3 months after PDT treatment, the expressions of MMP-9 and MMP-13(including mRNA and protein) in hypertrophic scar tissues of three treatment groups were significantly higher than those of the negative control group(P<0.01), and the expression of TIMP-1 mRNA and protein of three treatment groups were significantly lower than that of the negative control group(P<0.01). There were also significant differences between high-concentration ALA-PDT treatment group and the low one(P<0.05). Conclusion: ALA-PDT is effective in treating hypertrophic scars of rabbit ears, and its possible therapeutic mechanisms are that ALA-PDT treatment generates oxidation activation effect to activate the activity of MMPs and induces the photoaging of fibroblasts of hypertrophic scar tissues of rabbit ears to inhibit the activity of TIMPs, which causes the up-regulation of MMPs and the down-regulation of TIMPs. Because of this, the degradation of collagen and ECM is accelerated and the formation of scars is suppressed.
基金sponsored by the Youth Fund of National Natural Science Foundation of China(41302171)National Natural Science Foundation of China(41372345)
文摘We collect seismic moment tensors of the earthquakes occurring from 1900 to 2013 in and around the Chinese mainland and summarize the surface ruptures and displacements of 70 earthquakes with M S≥7. 0. We divide these large earthquakes into three types. Type A contains earthquakes with surface ruptures and displacements. Type B is earthquakes without displacements and Type C is those without any of this data. We simulate a triangular distribution of displacements for Type B and C. Then,we segment these large earthquakes by using their displacements and surface ruptures. Finally,kinematic models are determined from earthquake data and Bicubic Bessel spline functions. The results show that,first of all,the reasonability and spatial consistency of defined models are advanced.Strain rates have better continuity and are comparable with geologic and geodetic results in Himalaya thrust fault zones. The strain rates decrease in the Tarim basin and the Altun Tagh fault zones because of their low seismicity. The direction of compressional deformation in Gobi-Altay is changed from SE to NE and its extensional direction is changed from NE to NW. The extensional deformation in the Ordos block is diminished obviously. Secondly,earthquakes account for 30- 50% of expected motion of India relative to Eurasia determined from the NUVEL-1A model,with a missing component of 20 mm / a which may contain aseismic deformation such as fault creep and folds,the missing parts of earthquake data and elastic strain energy released by potential earthquakes.
基金jointly supported by National Science Foundation of China(41302171)Active Fault Exploration in China(60112304)Basic Scientific Research Funds of China Earthquake Administration(2014IES0401,2012IES010303)
文摘Since 2001, there have occurred in succession the 2001 Kunlun Mountains M S8. 1earthquake,the 2008 Wenchuan M S8. 0 earthquake,the 2010 Yushu M S7. 1 earthquake and the 2012 Lushan M S7. 0 earthquake in the periphery of the Bayan Har block. By comparison of the characteristics of seismic strain release variations before and after the Kunlun Mountains M S8. 1 earthquake in the same time length in the geodynamical related regions,we found that the seismic strain release was obviously enhanced after the earthquake in the Longmenshan area,Batang area,and the NS-trending valleys at the west of the Hot Spring Basin. The Wenchuan earthquake occurred in the first area,and the Yushu earthquake is related to the second area. After the earthquake rupture occurred on the East Kunlun fault zone on the northern boundary of the Bayan Har Block,crustal materials on the south side of the fault zone migrated to the southeast,leading to a concentration of tectonic deformation in the Longmenshan thrust belt, e ventually rupturing on the Longmenshan thrust belt. This earthquake case illustrates that seismicity enhancement zones are possibly prone to long-term destructive earthquakes. After the M S7. 3 earthquake in Yutian,Xinjiang on February 12,2014,earthquake frequency and seismic strain release markedly increased in the junction area between the eastern Qilian Mountain tectonic belt and the Altun Tagh fault zone,where more attention should be paid to the long-term seismic risk.
