目的探讨LIGHT(lymphotoxin like,exhibits inducible expression and competes with HSV glycoprotein D for HVEM,a receptor expressed by T lymphocytes)协同γ-干扰素(interferon-gamma,IFN-γ)诱导小鼠胰岛β细胞株MIN6细胞凋亡...目的探讨LIGHT(lymphotoxin like,exhibits inducible expression and competes with HSV glycoprotein D for HVEM,a receptor expressed by T lymphocytes)协同γ-干扰素(interferon-gamma,IFN-γ)诱导小鼠胰岛β细胞株MIN6细胞凋亡的作用及机制。方法 LIGHT、IFN-γ单独或联合处理MIN6细胞。分别运用CCK-8法、流式细胞术检测MIN6细胞增殖与凋亡率。Western blot检测信号转导和转录激活子1(signal transducers and activators of transcription 1,STAT1)及STAT1的磷酸化水平,以及凋亡与增殖相关基因Bcl-xL、Bax和增殖细胞核抗原(PCNA)的表达。MIN6细胞经STAT1特异的抑制剂弗达拉滨(fludarabine,Flu)预处理后再用LIGHT和IFN-γ联合处理,Western blot检测STAT1、p-STAT1、Bcl-xL、Bax的变化,CCK-8法检测MIN6细胞的增殖能力。结果 LIGHT协同IFN-γ呈时间依赖性抑制MIN6细胞增殖,诱导细胞凋亡,降低抗凋亡基因Bcl-xL表达、增加促凋亡基因Bax表达,对PCNA表达无明显影响;LIGHT促进IFN-γ激活STAT1,而STAT1抑制剂fludarabine抑制STAT1活化后,Bcl-xL表达上调,Bax表达下调,MIN6细胞增殖率上升。结论 LIGHT协同IFN-γ通过激活STAT1调节Bcl-xL、Bax表达而诱导小鼠胰岛β细胞株MIN6细胞凋亡。展开更多
The tectonic event during Cretaceous and its relationship with hydrocarbon accumulation in the Qiangtang Basin is discussed based on zircon U-Pb dating and the study of deformation, thermochronology and hydrocarbon fo...The tectonic event during Cretaceous and its relationship with hydrocarbon accumulation in the Qiangtang Basin is discussed based on zircon U-Pb dating and the study of deformation, thermochronology and hydrocarbon formation. LA-ICPMS zircon U-Pb dating indicates that the tectonic event took place during the Early-Late Cretaceous （125-75Ma）. The event not only established the framework and the styles of structural traps in the basin, but also led to the cessation of the first hydrocarbon formation and the destruction of previous oil pools. The light crude oil in the basin was formed during the second hydrocarbon formation stage in the Cenozoic, and ancient structural traps formed during the Cretaceous event are promising targets for oil and gas exploration.展开更多
In this study,zircon U-Pb dating of volcanic rocks from the Zhongba ophiolite of the Yarlung Zangbo Suture Zone(YZSZ)in southern Xizang(Tibet)yielded an age of 247±3 Ma.According to whole rock geochemical and Sr-...In this study,zircon U-Pb dating of volcanic rocks from the Zhongba ophiolite of the Yarlung Zangbo Suture Zone(YZSZ)in southern Xizang(Tibet)yielded an age of 247±3 Ma.According to whole rock geochemical and Sr-NdPb isotopic data,the Early Triassic samples could be divided into two groups:Group 1 with P-MORB affinity,showing initial^(87)Sr/^(86)Sr ratios of 0.70253–0.70602,ε_(Nd)(t)values of 4.2–5.3,(^(206)Pb/^(204)Pb)_(t)ratios of 16.353–18.222,(^(207)Pb/^(204)Pb)_(t)ratios of 15.454–15.564,and(^(208)Pb/^(204)Pb)_(t)ratios of 35.665–38.136;Group 2 with OIB affinity,showing initial^(87)Sr/^(86)Sr ratios of 0.70249–0.70513,ε_(Nd)(t)values of 4.4–4.9,(^(206)Pb/^(204)Pb)_(t)ratios of 17.140–18.328,(^(207)Pb/^(204)Pb)_(t)ratios of 15.491–15.575,and(^(208)Pb/^(204)Pb)_(t)ratios of 36.051–38.247.Group 2 rocks formed by partial melting of the mantle source enriched by a former plume,and assimilated continental crustal material during melt ascension.The formation of Group 1 rocks corresponds to the mixing of OIB melts,with the same components as Group 2 and N-MORBs.The Zhongba Early Triassic rocks belong to the continental margin type ophiolite and formed in the continental–oceanic transition zone during the initial opening of the Neo-Tethys in southern Xizang(Tibet).展开更多
The Longwangmiao Fm gas pool was found in 2012 in Moxi area in Leshan-Longnüsi Paleouplift,central Sichuan Basin.Previous geological studies showed that the Lower Cambrian Longwangmiao Fm reservoir is mainly pore...The Longwangmiao Fm gas pool was found in 2012 in Moxi area in Leshan-Longnüsi Paleouplift,central Sichuan Basin.Previous geological studies showed that the Lower Cambrian Longwangmiao Fm reservoir is mainly pore type reservoir of grain shoal facies,locally superimposed by the vuggy reservoir formed by Caledonian karstification,and the distribution of the reservoir has a critical control effect on the gas pool there.Therefore,the reservoir prediction in this area follows the approach of“looking for the overlap of shoal facies,karst,bright spots,and traps”.First,the favorable facies boundary and karstification effect range are defined based on sedimentary facies;on the basis of precise correlation of the top and bottom horizons of the reservoir,the favorable development area of the reservoir was then predicted according to the seismic response characteristics of“bright spots”of the Longwangmiao Fm reservoir;finally,favorable exploration areas were selected according to the effective configuration of reservoir and trap.Due to the horizontal change in reservoir and lithology,Longwangmiao Fm top has no uniform interface features on the seismic profile,making it difficult to trace.In the actual interpretation process,the underlying reference horizon is sought out first,then the bottom boundary of Longwangmiao Fm is correlated,and finally its top boundary is correlated.The prediction results indicated that the Longwangmiao reservoir distributes in band shape in a wide range around the Paleouplift;and the exploration prospects mainly involve three domains:lithologic-stratigraphic traps near the pinchout line of Longwangmiao Fm;lithologic traps formed by isolated shoal body;and structural and lithologic traps at the north flank of the Paleouplift.展开更多
The India-Asia collision and uplift of the Tibetan plateau are the most important geological events in Earth＇s history; it profoundly affects our understanding of global climate change during the Cenozoic. Despite a ...The India-Asia collision and uplift of the Tibetan plateau are the most important geological events in Earth＇s history; it profoundly affects our understanding of global climate change during the Cenozoic. Despite a research history over half a century, the uplifting history remains hotly controversial. The early uplifting model suggested that the Lhasa terrane attained an elevation of 3-4 km at -99 Ma due to the Lhasa-Qiangtang collision. However, the evidences are mainly from deformation of the Lhasa terrane and inconsistency with the palaeontological data.展开更多
The Cretaceous Xigaze ophiolite is best exposed at the central Yarlung Zangbo Suture Zone （YZSZ, Tibet） which also includes the Gangdese arc and the Xigaze forearc basin. This study reports new geochronological and ...The Cretaceous Xigaze ophiolite is best exposed at the central Yarlung Zangbo Suture Zone （YZSZ, Tibet） which also includes the Gangdese arc and the Xigaze forearc basin. This study reports new geochronological and geochemical data for this ophiolite to revisit its geodynamic and petrogenetic evolution.展开更多
文摘目的探讨LIGHT(lymphotoxin like,exhibits inducible expression and competes with HSV glycoprotein D for HVEM,a receptor expressed by T lymphocytes)协同γ-干扰素(interferon-gamma,IFN-γ)诱导小鼠胰岛β细胞株MIN6细胞凋亡的作用及机制。方法 LIGHT、IFN-γ单独或联合处理MIN6细胞。分别运用CCK-8法、流式细胞术检测MIN6细胞增殖与凋亡率。Western blot检测信号转导和转录激活子1(signal transducers and activators of transcription 1,STAT1)及STAT1的磷酸化水平,以及凋亡与增殖相关基因Bcl-xL、Bax和增殖细胞核抗原(PCNA)的表达。MIN6细胞经STAT1特异的抑制剂弗达拉滨(fludarabine,Flu)预处理后再用LIGHT和IFN-γ联合处理,Western blot检测STAT1、p-STAT1、Bcl-xL、Bax的变化,CCK-8法检测MIN6细胞的增殖能力。结果 LIGHT协同IFN-γ呈时间依赖性抑制MIN6细胞增殖,诱导细胞凋亡,降低抗凋亡基因Bcl-xL表达、增加促凋亡基因Bax表达,对PCNA表达无明显影响;LIGHT促进IFN-γ激活STAT1,而STAT1抑制剂fludarabine抑制STAT1活化后,Bcl-xL表达上调,Bax表达下调,MIN6细胞增殖率上升。结论 LIGHT协同IFN-γ通过激活STAT1调节Bcl-xL、Bax表达而诱导小鼠胰岛β细胞株MIN6细胞凋亡。
基金supported by the National Natural Science Foundation of China (40672086)the Ministry of Science and Technology "973" Project (2006CB701400)the Ministry of Land and Resources (XQ2004-06)
文摘The tectonic event during Cretaceous and its relationship with hydrocarbon accumulation in the Qiangtang Basin is discussed based on zircon U-Pb dating and the study of deformation, thermochronology and hydrocarbon formation. LA-ICPMS zircon U-Pb dating indicates that the tectonic event took place during the Early-Late Cretaceous （125-75Ma）. The event not only established the framework and the styles of structural traps in the basin, but also led to the cessation of the first hydrocarbon formation and the destruction of previous oil pools. The light crude oil in the basin was formed during the second hydrocarbon formation stage in the Cenozoic, and ancient structural traps formed during the Cretaceous event are promising targets for oil and gas exploration.
基金the National Natural Science Foundation of China(Grant Nos.91955206,41603038)Second Tibetan Plateau Scientific Expedition and Research program(Grant No.2019QZKK0803)+2 种基金Scientific Research Foundation for Advanced ScholarsWest Yunnan University of Applied Sciences(Grant No.2022RCKY0004)Yunnan Fundamental Research Projects(Grant No.202301AT070012).
文摘In this study,zircon U-Pb dating of volcanic rocks from the Zhongba ophiolite of the Yarlung Zangbo Suture Zone(YZSZ)in southern Xizang(Tibet)yielded an age of 247±3 Ma.According to whole rock geochemical and Sr-NdPb isotopic data,the Early Triassic samples could be divided into two groups:Group 1 with P-MORB affinity,showing initial^(87)Sr/^(86)Sr ratios of 0.70253–0.70602,ε_(Nd)(t)values of 4.2–5.3,(^(206)Pb/^(204)Pb)_(t)ratios of 16.353–18.222,(^(207)Pb/^(204)Pb)_(t)ratios of 15.454–15.564,and(^(208)Pb/^(204)Pb)_(t)ratios of 35.665–38.136;Group 2 with OIB affinity,showing initial^(87)Sr/^(86)Sr ratios of 0.70249–0.70513,ε_(Nd)(t)values of 4.4–4.9,(^(206)Pb/^(204)Pb)_(t)ratios of 17.140–18.328,(^(207)Pb/^(204)Pb)_(t)ratios of 15.491–15.575,and(^(208)Pb/^(204)Pb)_(t)ratios of 36.051–38.247.Group 2 rocks formed by partial melting of the mantle source enriched by a former plume,and assimilated continental crustal material during melt ascension.The formation of Group 1 rocks corresponds to the mixing of OIB melts,with the same components as Group 2 and N-MORBs.The Zhongba Early Triassic rocks belong to the continental margin type ophiolite and formed in the continental–oceanic transition zone during the initial opening of the Neo-Tethys in southern Xizang(Tibet).
基金Major Project of National Science and Technology(No.2011ZX05004-005)Major Project of Exploration of PetroChina“Hydrocarbon Evaluation of Leshan-Longnüsi Palaeohigh in Sichuan Basin and Research on Exploration Support Technologies”(No.2012ZD01-03-01)。
文摘The Longwangmiao Fm gas pool was found in 2012 in Moxi area in Leshan-Longnüsi Paleouplift,central Sichuan Basin.Previous geological studies showed that the Lower Cambrian Longwangmiao Fm reservoir is mainly pore type reservoir of grain shoal facies,locally superimposed by the vuggy reservoir formed by Caledonian karstification,and the distribution of the reservoir has a critical control effect on the gas pool there.Therefore,the reservoir prediction in this area follows the approach of“looking for the overlap of shoal facies,karst,bright spots,and traps”.First,the favorable facies boundary and karstification effect range are defined based on sedimentary facies;on the basis of precise correlation of the top and bottom horizons of the reservoir,the favorable development area of the reservoir was then predicted according to the seismic response characteristics of“bright spots”of the Longwangmiao Fm reservoir;finally,favorable exploration areas were selected according to the effective configuration of reservoir and trap.Due to the horizontal change in reservoir and lithology,Longwangmiao Fm top has no uniform interface features on the seismic profile,making it difficult to trace.In the actual interpretation process,the underlying reference horizon is sought out first,then the bottom boundary of Longwangmiao Fm is correlated,and finally its top boundary is correlated.The prediction results indicated that the Longwangmiao reservoir distributes in band shape in a wide range around the Paleouplift;and the exploration prospects mainly involve three domains:lithologic-stratigraphic traps near the pinchout line of Longwangmiao Fm;lithologic traps formed by isolated shoal body;and structural and lithologic traps at the north flank of the Paleouplift.
基金financially supported by the National Natural Science Foundation of China(41572188)the China Geological Survey(DD20160159-06)
文摘The India-Asia collision and uplift of the Tibetan plateau are the most important geological events in Earth＇s history; it profoundly affects our understanding of global climate change during the Cenozoic. Despite a research history over half a century, the uplifting history remains hotly controversial. The early uplifting model suggested that the Lhasa terrane attained an elevation of 3-4 km at -99 Ma due to the Lhasa-Qiangtang collision. However, the evidences are mainly from deformation of the Lhasa terrane and inconsistency with the palaeontological data.
文摘The Cretaceous Xigaze ophiolite is best exposed at the central Yarlung Zangbo Suture Zone （YZSZ, Tibet） which also includes the Gangdese arc and the Xigaze forearc basin. This study reports new geochronological and geochemical data for this ophiolite to revisit its geodynamic and petrogenetic evolution.
