The Wiener index of a graph is defined to be the sum of the distances of all pairs of vertices in the graph.The kth power G^(k) of a graph G is the graph on V(G)and two vertices are adjacent if and only if their dista...The Wiener index of a graph is defined to be the sum of the distances of all pairs of vertices in the graph.The kth power G^(k) of a graph G is the graph on V(G)and two vertices are adjacent if and only if their distance in G is less or equal to k.In this paper,we computed the Wiener index of the kth power of paths and cycles for any k≥2.展开更多
Salidroside(SAL),a major bioactive compound of Rhodiola crenulata,has significant anti-hypoxia effect,however,its underlying molecular mechanism has not been elucidated.In order to explore the protective mechanism of ...Salidroside(SAL),a major bioactive compound of Rhodiola crenulata,has significant anti-hypoxia effect,however,its underlying molecular mechanism has not been elucidated.In order to explore the protective mechanism of SAL,the lactate dehydrogenase(LDH),reactive oxygen species(ROS),superoxide dismutase(SOD)and hypoxia-induced factor 1α(HIF-1α)were measured to establish the PC12 cell hypoxic model.Cell staining and cell viability analyses were performed to evaluate the protective effects of SAL.The metabolomics and bioinformatics methods were used to explore the protective effects of salidroside under hypoxia condition.The metabolite-protein interaction networks were further established and the protein expression level was examined by Western blotting.The results showed that 59 endogenous metabolites changed and the expression of the hub proteins of CK2,p-PTEN/PTEN,PI3K,p-Akt/Akt,NF-κB p65 and Bcl-2 were increased,suggesting that SAL could increase the expression of CK2,which induced the phosphorylation and inactivation of PTEN,reduced the inhibitory effect on PI3K signaling pathways and activated the PI3K/Akt/NF-κB survival signaling pathway.Our study provided an important insight to reveal the protective molecular mechanism of SAL as a novel drug candidate.展开更多
OBJECTIVE The aim of this study is to investigate the inhibitory effects of lapachol on rat C6 glioma both in vitro and in vivo,as well as the potential mechanisms.METHODS First,the model of C6 glioma in Wistar rats w...OBJECTIVE The aim of this study is to investigate the inhibitory effects of lapachol on rat C6 glioma both in vitro and in vivo,as well as the potential mechanisms.METHODS First,the model of C6 glioma in Wistar rats was established and verified by hemotoxylin and eosin staining,immunohistochemical staining and magnetic resonance imaging(MRI).Then different doses of lapachol were gavaged and tumor volumes of the C6 glioma were detected by MRI.The effects of lapachol on C6 cell proliferation,apoptosis and DNA damage were detected by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS)/phen-azinemethosulfate(PMS)assay,Hoechst33358 staining,AnnexinⅤ-FITC/PI staining,and comet assay.Effects of lapachol on topoisomeraseⅠ(TOPⅠ)and topoisomeraseⅡ(TOPⅡ)activities were detected by TOPⅠand TOPⅡmediated supercoiled p BR322 DNA relaxation assay.Molecular docking was used to predict the interaction of lapachol-TOPⅠand lapachol-TOPⅡ.TOP I and TOPⅡexpression levels in C6 cells were determined by Enzymelinked immunosorbent assay kits and real-time polymerase chain reaction(RT-PCR).RESULTS The rat C6 glioma model was successfully established.High dose lapachol showed significant inhibitory effect on the C6 glioma in Wistar rats(P<0.05).MTS/PMS assay,Hoechst 33258 staining,AnnexinⅤ-FITC/PI staining,and comet assay showed that lapachol could inhibit proliferation,induce apoptosis and DNA damage of C6 cells in dose dependent manners.Lapachol could inhibit the activities of both TOPⅠandⅡ.Molecular docking showed that lapachol-TOPⅠshowed relatively stronger interaction than that of lapachol-TOPⅡ.Enzyme-linked immunosorbent assay and RT-PCR showed that lapachol could inhibit TOPⅡexpression levels,but not TOPⅠexpression levels.CONCLUSION These results showed that lapachol could significantly inhibit C6 glioma both in vivo and in vitro,which might be related with inhibiting TOPⅠand TOPⅡactivities,as wel as TOPⅡexpression.展开更多
OBJECTIVE Hypoxia is associated with many complicated pathophysiological and biochemical processes that integrated and regulated via the key gene,protein and endogenous metabolite levels.Up to date,the exact molecular...OBJECTIVE Hypoxia is associated with many complicated pathophysiological and biochemical processes that integrated and regulated via the key gene,protein and endogenous metabolite levels.Up to date,the exact molecular mechanism of hypoxia still remains unclear.In this work,we further explore the molecular mechanism of hypoxia and adaption to attenuate the damage in zebrafish model that have potential to resist hypoxic environment.METHODS The hypoxic zebrafish model was established in different concentration of oxygen with 3%,5%,10%,21%in water.The brain tissue was separated and the RNA-seq was used to identify the differentially expressed genes.The related endogenous metabolites profiles were obtained by LC-HDMS,and the multivariate statistics was applied to discover the important metabolites candidates in hypoxic zebrafish.The candidates were searched in HMDB,KEGG and Lipid Maps databases.RESULTS The zebrafish hypoxic model was successfully constructed via the different concentration of oxygen,temperature and hypoxic time.The activities of the related hypoxic metabolic enzymes and factors including HIF-1a,actate dehydrogenase(LDH)and citrate synthase(CS)were evaluated.Significant differences(P<0.05 and fold change>2)in the expression of 422 genes were observed between the normal and 3% hypoxic model.Among them,201 genes increased depended on the lower concentration of oxygen.53 metabolites were identified that had significant difference between the hypoxia and control groups(P<0.05,fold change>1.5 and VIP>1.5).The ten key metabolites were increased gradually while six compounds were decreased.The endogenous hypoxic metabolites of phenylalanine,D-glucosamine-6P and several important lipids with the relevant hub genes had similar change in hypoxic model.In addition,the metabolic pathways of phenylalanine,glutamine and glycolipid were influenced in both the levels of genes and metabolites.CONCLUSION The up-regulation of phenylalanine,D-glucosamine-6P and lipid may have further understanding of protective effect in hypoxia.Our data provided an insight to further reveal the hypoxia and adaptation mechanism.展开更多
The acoustic nonlinearity parameter B/A of a liquid containing microbubbles,sonicated dextrose albumin,has been measured by using second harmonic insert-substitution method.Results show that the value of nonlinearity ...The acoustic nonlinearity parameter B/A of a liquid containing microbubbles,sonicated dextrose albumin,has been measured by using second harmonic insert-substitution method.Results show that the value of nonlinearity parameter B/A for a bubbly liquid is significant and it obviously increases with increase of bubble number density.The high nonlinearity of the bubbly liquid is mainly dependent on the microbubble nonlinear oscillation.展开更多
血栓性疾病是导致人类死亡的主要原因。沉默信息调节因子1(silent information regulator 1,SIRT1)调节炎症反应、氧化应激、血管生成、能量代谢以及内皮细胞功能等过程,在静脉血栓栓塞、缺血性脑卒中、心房颤动、动脉粥样硬化等血栓性...血栓性疾病是导致人类死亡的主要原因。沉默信息调节因子1(silent information regulator 1,SIRT1)调节炎症反应、氧化应激、血管生成、能量代谢以及内皮细胞功能等过程,在静脉血栓栓塞、缺血性脑卒中、心房颤动、动脉粥样硬化等血栓性疾病中发挥重要作用。本文就SIRT1的结构和生理功能,以及其参与血栓性疾病的机制和研究进展进行综述。展开更多
基金Supported by National Natural Science Foundation of China(Grant No.12201471)the Special Foundation in Key Fields for Universities of Guangdong Province(Grant No.2022ZDZX1034).
文摘The Wiener index of a graph is defined to be the sum of the distances of all pairs of vertices in the graph.The kth power G^(k) of a graph G is the graph on V(G)and two vertices are adjacent if and only if their distance in G is less or equal to k.In this paper,we computed the Wiener index of the kth power of paths and cycles for any k≥2.
基金supported by the National Natural Science Foundation of China(Nos.81573683,81173121,81773803).
文摘Salidroside(SAL),a major bioactive compound of Rhodiola crenulata,has significant anti-hypoxia effect,however,its underlying molecular mechanism has not been elucidated.In order to explore the protective mechanism of SAL,the lactate dehydrogenase(LDH),reactive oxygen species(ROS),superoxide dismutase(SOD)and hypoxia-induced factor 1α(HIF-1α)were measured to establish the PC12 cell hypoxic model.Cell staining and cell viability analyses were performed to evaluate the protective effects of SAL.The metabolomics and bioinformatics methods were used to explore the protective effects of salidroside under hypoxia condition.The metabolite-protein interaction networks were further established and the protein expression level was examined by Western blotting.The results showed that 59 endogenous metabolites changed and the expression of the hub proteins of CK2,p-PTEN/PTEN,PI3K,p-Akt/Akt,NF-κB p65 and Bcl-2 were increased,suggesting that SAL could increase the expression of CK2,which induced the phosphorylation and inactivation of PTEN,reduced the inhibitory effect on PI3K signaling pathways and activated the PI3K/Akt/NF-κB survival signaling pathway.Our study provided an important insight to reveal the protective molecular mechanism of SAL as a novel drug candidate.
文摘OBJECTIVE The aim of this study is to investigate the inhibitory effects of lapachol on rat C6 glioma both in vitro and in vivo,as well as the potential mechanisms.METHODS First,the model of C6 glioma in Wistar rats was established and verified by hemotoxylin and eosin staining,immunohistochemical staining and magnetic resonance imaging(MRI).Then different doses of lapachol were gavaged and tumor volumes of the C6 glioma were detected by MRI.The effects of lapachol on C6 cell proliferation,apoptosis and DNA damage were detected by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS)/phen-azinemethosulfate(PMS)assay,Hoechst33358 staining,AnnexinⅤ-FITC/PI staining,and comet assay.Effects of lapachol on topoisomeraseⅠ(TOPⅠ)and topoisomeraseⅡ(TOPⅡ)activities were detected by TOPⅠand TOPⅡmediated supercoiled p BR322 DNA relaxation assay.Molecular docking was used to predict the interaction of lapachol-TOPⅠand lapachol-TOPⅡ.TOP I and TOPⅡexpression levels in C6 cells were determined by Enzymelinked immunosorbent assay kits and real-time polymerase chain reaction(RT-PCR).RESULTS The rat C6 glioma model was successfully established.High dose lapachol showed significant inhibitory effect on the C6 glioma in Wistar rats(P<0.05).MTS/PMS assay,Hoechst 33258 staining,AnnexinⅤ-FITC/PI staining,and comet assay showed that lapachol could inhibit proliferation,induce apoptosis and DNA damage of C6 cells in dose dependent manners.Lapachol could inhibit the activities of both TOPⅠandⅡ.Molecular docking showed that lapachol-TOPⅠshowed relatively stronger interaction than that of lapachol-TOPⅡ.Enzyme-linked immunosorbent assay and RT-PCR showed that lapachol could inhibit TOPⅡexpression levels,but not TOPⅠexpression levels.CONCLUSION These results showed that lapachol could significantly inhibit C6 glioma both in vivo and in vitro,which might be related with inhibiting TOPⅠand TOPⅡactivities,as wel as TOPⅡexpression.
基金supported by National Natural Science Foundation of China(81573683 and 81173121)
文摘OBJECTIVE Hypoxia is associated with many complicated pathophysiological and biochemical processes that integrated and regulated via the key gene,protein and endogenous metabolite levels.Up to date,the exact molecular mechanism of hypoxia still remains unclear.In this work,we further explore the molecular mechanism of hypoxia and adaption to attenuate the damage in zebrafish model that have potential to resist hypoxic environment.METHODS The hypoxic zebrafish model was established in different concentration of oxygen with 3%,5%,10%,21%in water.The brain tissue was separated and the RNA-seq was used to identify the differentially expressed genes.The related endogenous metabolites profiles were obtained by LC-HDMS,and the multivariate statistics was applied to discover the important metabolites candidates in hypoxic zebrafish.The candidates were searched in HMDB,KEGG and Lipid Maps databases.RESULTS The zebrafish hypoxic model was successfully constructed via the different concentration of oxygen,temperature and hypoxic time.The activities of the related hypoxic metabolic enzymes and factors including HIF-1a,actate dehydrogenase(LDH)and citrate synthase(CS)were evaluated.Significant differences(P<0.05 and fold change>2)in the expression of 422 genes were observed between the normal and 3% hypoxic model.Among them,201 genes increased depended on the lower concentration of oxygen.53 metabolites were identified that had significant difference between the hypoxia and control groups(P<0.05,fold change>1.5 and VIP>1.5).The ten key metabolites were increased gradually while six compounds were decreased.The endogenous hypoxic metabolites of phenylalanine,D-glucosamine-6P and several important lipids with the relevant hub genes had similar change in hypoxic model.In addition,the metabolic pathways of phenylalanine,glutamine and glycolipid were influenced in both the levels of genes and metabolites.CONCLUSION The up-regulation of phenylalanine,D-glucosamine-6P and lipid may have further understanding of protective effect in hypoxia.Our data provided an insight to further reveal the hypoxia and adaptation mechanism.
基金Supported by the National Natural Science Foundation of China under Grant No.19834040.
文摘The acoustic nonlinearity parameter B/A of a liquid containing microbubbles,sonicated dextrose albumin,has been measured by using second harmonic insert-substitution method.Results show that the value of nonlinearity parameter B/A for a bubbly liquid is significant and it obviously increases with increase of bubble number density.The high nonlinearity of the bubbly liquid is mainly dependent on the microbubble nonlinear oscillation.
文摘血栓性疾病是导致人类死亡的主要原因。沉默信息调节因子1(silent information regulator 1,SIRT1)调节炎症反应、氧化应激、血管生成、能量代谢以及内皮细胞功能等过程,在静脉血栓栓塞、缺血性脑卒中、心房颤动、动脉粥样硬化等血栓性疾病中发挥重要作用。本文就SIRT1的结构和生理功能,以及其参与血栓性疾病的机制和研究进展进行综述。
