BACKGROUND Uterine injury can cause uterine scarring,leading to a series of complications that threaten women’s health.Uterine healing is a complex process,and there are currently no effective treatments.Although our...BACKGROUND Uterine injury can cause uterine scarring,leading to a series of complications that threaten women’s health.Uterine healing is a complex process,and there are currently no effective treatments.Although our previous studies have shown that bone marrow mesenchymal stem cells(BMSCs)promote uterine damage repair,the underlying mechanisms remain unclear.However,exploring the specific regulatory roles of BMSCs in uterine injury treatment is crucial for further understanding their functions and enhancing therapeutic efficacy.AIM To investigate the underlying mechanism by which BMSCs promote the process of uterine healing.METHODS In in vivo experiments,we established a model of full-thickness uterine injury and injected BMSCs into the uterine wound.Transcriptome sequencing was per-formed to determine the enrichment of differentially expressed genes at the wound site.In in vitro experiments,we isolated rat uterine smooth muscle cells(USMCs)and cocultured them with BMSCs to observe the interaction between BMSCs and USMCs in the microenvironment.RESULTS We found that the differentially expressed genes were mainly related to cell growth,tissue repair,and angiogenesis,while the phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)pathway was highly enriched.Quantitative reverse-transcription polymerase chain reaction was used to validate differentially expressed genes,and the results demonstrated that BMSCs can upregulate genes related to regeneration and downregulate genes related to inflammation.Coculturing BMSCs promoted the migration and proliferation of USMCs,and the USMC microenvironment promoted the myogenic differentiation of BMSCs.Finally,we validated the PI3K/AKT pathway in tissues and cells and showed that BMSCs activate the PI3K/AKT pathway to promote the regeneration of uterine smooth muscle both in vivo and in vitro.CONCLUSION BMSCs upregulated uterine wound regeneration and anti-inflammatory factors and enhanced uterine smooth muscle proliferation through the PI3K/AKT pathway both in vivo and in vitro.展开更多
A DFN-DEC(discrete fracture network-distinct element code)method based on the MATLAB platform is developed to generate heterogeneous DFN.Subsequently,the effects of the spatial variability(the meanμand the standard d...A DFN-DEC(discrete fracture network-distinct element code)method based on the MATLAB platform is developed to generate heterogeneous DFN.Subsequently,the effects of the spatial variability(the meanμand the standard deviationσ)of the geometric properties(i.e.,the fracture dip D,the trace length T and the spacing S)of both the gently-dipping(denoted with 1)and the steeply-dipping(denoted with 2)fractures on the stability of granite slope are investigated.Results indicate that the proposed DFN-DEC method is robust,generating fracture networks that resemble reality.In addition,the spatial variability of fracture geometry,influencing the structure of granite slope,plays a significant role in slope stability.The mean stability of the slope decreases with the increase ofμ_(D_(1))(the mean of gently-dipping fracture dip),σ_(D_(2))(the mean of steeply-dipping fracture dip),μ_(T_(1))(the mean of gently-dipping fracture trace length),μ_(T_(2))(the mean of steeply-dipping fracture trace length),σ_(T_(1))(the standard deviation of gently-dipping fracture trace length),σ_(T_(2))(the standard deviation of steeply-dipping fracture trace length),and the decrease ofσ_(D_(1))(the standard deviation of gently-dipping fracture dip),μ_(D_(2))(the standard deviation of steeply-dipping fracture dip),μ_(S_(1))(the mean of gently-dipping fracture spacing)andμ_(S_(2))(the mean of steeply-dipping fracture spacing).Among them,μ_(T_(1)),μ_(D_(1))andμ_(S_(1))have the major impact.When the fracture spacing is large,the variability in the fracture geometry becomes less relevant to slope stability.When within some ranges of the fracture spacing,the spatial varying of dips can increase the slope stability by forming an interlaced structure.The results also show that the effects of the variability of trace length on slope stability depend on the variability of dip.These findings highlight the importance of spatial variability in the geometry of fractures to rock slope stability analysis.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is a significant global health challenge with rising incidence rates and poor prognoses.Aminoacyl-tRNA synthetases(ARSs)are important regulators implicated in the occurrence and...BACKGROUND Hepatocellular carcinoma(HCC)is a significant global health challenge with rising incidence rates and poor prognoses.Aminoacyl-tRNA synthetases(ARSs)are important regulators implicated in the occurrence and progression of several cancers.However,their specific function in HCC remains unclear,and ARSs-related prognostic factors for patient stratification are lacking.AIM To investigate the ARSs-related mechanisms of HCC and establish an effective prognostic risk model for stratifying patients with HCC.METHODS We screened ARSs genes of interest using differential gene expression,mutation,and survival analysis.Western blot and Immunohistochemistry were used to analyze MARS1 expression in the liver tissues of patients with HCC.Functional studies,including CCK-8 cell viability assay,EdU cell proliferation assay,cell cycle assays,Transwell migration and invasion assays,and in vivo tumor xenograft models,were conducted to comprehensively elucidate the specific role of MARS1 in HCC.Moreover,the MARS1-related prognostic score(MRPS)was established by LASSO regression and Cox regression analysis in The Cancer Genome Atlas-HCC and GSE14520 cohorts.Patients’immunotherapy and chemotherapy responses were predicted by immunomicroenvironment and drug susceptibility analysis in both subgroups.RESULTS MARS1 was selected as a target gene from a series of ARSs genes,with markedly higher expression observed in HCC tissues compared to adjacent non-cancerous tissues.Silencing MARS1 considerably impeded the proliferation,migration,invasion,and tumorigenic abilities of HCC cells in vitro and in vivo.Moreover,high MRPSs were associated with poor overall survival,altered infiltration of T cells,macrophages,monocytes,elevated immune checkpoint expression(PD-L1,CTLA4,LAG3),and reduced drug sensitivity in HCC.CONCLUSION MARS1 promotes HCC development and represents a potential therapeutic target for HCC management.Furthermore,MRPS serves as an independent prognostic factor for survival and a predictor of tumor treatment response.展开更多
BACKGROUND Diabetic gastrointestinal autonomic neuropathy(DGAN)often affects the patients’quality of life.Thus,research on influencing factors of DGAN may promote DGAN prevention and decrease the incidence rate.This ...BACKGROUND Diabetic gastrointestinal autonomic neuropathy(DGAN)often affects the patients’quality of life.Thus,research on influencing factors of DGAN may promote DGAN prevention and decrease the incidence rate.This study used electrogastrogram(EGG)to assess the relationship between metabolic dysfunction-associated steatotic liver disease(MASLD)and DGAN.AIM To analyze the changes of EGG in patients with MASLD and type 2 diabetes mellitus(T2DM),and to elucidate whether ultrasound-diagnosed MASLD is an independent risk factor for diabetic gastric motility disorders(DGMD).METHODS A total of 272 patients with T2DM hospitalized at the Second Affiliated Hospital of Soochow University from December 2020 to December 2021 and the Affiliated Suqian Hospital of Xuzhou Medical University from November 2023 to June 2024 were included in the cross-sectional study.General information,clinical data,and medical history of all study subjects,including name,age,gender,body mass index,and duration of diabetes were collected.Laboratory tests included biochemical parameters,glycosylated hemoglobin(HbA1c),fasting C-peptide,2h postprandial C-peptide and 25-hydroxyvitamin D[25(OH)D].EGG,fundus examination and carotid artery ultrasonography were performed and results were recorded.According to the results of EGG,the subjects were divided into the DGMD group and non-DGMD group.RESULTS The duration of diabetes,fasting blood glucose(FBG),HbA1c,25(OH)D and the prevalence of MASLD were significantly higher in the DGMD group(P<0.05).Multiple logistic regression analysis showed that the duration,FBG,25(OH)D and the presence of MASLD were independent influencing factors.CONCLUSION MASLD is strongly associated with an increased incidence of DGMD.Timely treatment of MASLD is effective to prevent diabetic gastroparesis.展开更多
The chief objective of the article is to learn the spatial characteristics of stress distribution around a shallow buried cylinder Karst cave in limestone strata.Firstly,taking into account the geometry of limestone f...The chief objective of the article is to learn the spatial characteristics of stress distribution around a shallow buried cylinder Karst cave in limestone strata.Firstly,taking into account the geometry of limestone formations,and the characteristics of Karst geomorphology in China,a spatial axialsymmetrical hollow model was established.Concurrently,combining available work and the concept of elasticity,the boundary conditions are determined.Subsequently,Love displacement method was introduced,the expressions of stress components were gained.The diagram characteristics of each stress component are summarized,which are affected by various influencing factors.Finally,in order to prove the rationality of the general solution,numerical simulation was carried out on the basis of practical engineering,and the maximum error is less than 5%.Thus,the analytical solution could represent the spatial characteristics of stress distribution around a shallow buried cylinder Karst cave in limestone strata.展开更多
Jing Medicine, as an important part of China’s ethnic medicine and culture, is characterized by its unique theories, rich content, and abundant resources, showcasing distinctive features of minority regions. The Jing...Jing Medicine, as an important part of China’s ethnic medicine and culture, is characterized by its unique theories, rich content, and abundant resources, showcasing distinctive features of minority regions. The Jing ethnic group is distributed in coastal and border areas of China, and after thousands of years of development, it has formed a rich marine-oriented medical system in the struggle against the environment and diseases, displaying unique medication characteristics and clinical efficacy. According to Jing Medicine, the human body is an organic whole that is unified with the natural and social environment. It believes that the heavens and earth are the spirits of all things, and all things possess spiritual energy, which is closely connected to the human body’s spiritual energy. The utilization of marine organisms in medicine is the greatest feature of Jing Medicine, and it is a common approach used by the local population for disease prevention and treatment. To further explore Jing Medicine, improve the repository of ethnic medicine culture, and address the insufficient understanding of Jing Medicine in traditional Chinese medicine culture, this article provides a comprehensive review of the distinctive diagnostic and therapeutic theories, techniques, and applications of internal and external treatments in Jing Medicine.展开更多
Objective: To observe the clinical efficacy of a Jing ethnic prescription in treating chronic atrophic gastritis (CAG). Methods: A total of 182 CAG patients admitted to our hospital were randomly divided into a contro...Objective: To observe the clinical efficacy of a Jing ethnic prescription in treating chronic atrophic gastritis (CAG). Methods: A total of 182 CAG patients admitted to our hospital were randomly divided into a control group (91 cases) and a treatment group (91 cases) based on their admission order. The control group received conventional treatment, while the treatment group was treated with a Jing ethnic prescription. The clinical efficacy, changes in gastric mucosa-related indicators, and systemic inflammatory markers were compared between the two groups. Results: After treatment, the overall effective rate in the treatment group was higher than that in the control group, but the difference was not statistically significant (P > 0.05). The levels of trefoil factor 2 (TFF2) in the gastric mucosa increased, and the levels of nuclear factor-kappa B (NF-κB) decreased in both groups. However, the improvement in these indicators was significantly better in the treatment group (P Conclusion: The custom formula of the Jing ethnic group shows comparable clinical efficacy to conventional treatment for chronic atrophic gastritis (CAG), but it demonstrates significantly better effects in reducing systemic inflammatory responses. Specifically, the treatment group showed superior results in the following aspects compared to the control group: increased levels of TFF2, decreased levels of NF-κB, and reduced serum levels of IL-6, IL-8, and hs-CRP.展开更多
Objective: To explore the clinical efficacy of the Jing ethnic group’s self-proposed formula combined with burning mugwort on patients after cholecystectomy for gallstones. Methods: Sixty-four patients with gallstone...Objective: To explore the clinical efficacy of the Jing ethnic group’s self-proposed formula combined with burning mugwort on patients after cholecystectomy for gallstones. Methods: Sixty-four patients with gallstones who received inpatient treatment at the Hepatobiliary Surgery Department of Fangchenggang Traditional Chinese Medicine Hospital from November 2022 to November 2023 were selected and randomly divided into a control group and an observation group. The treatment group was treated with the Jing ethnic group’s self-proposed formula combined with burning mugwort, while the control group was treated with ursodeoxycholic acid tablets. The liver function indicators, therapeutic effect, safety evaluation, and quality of life of the two groups were compared. Results: After treatment, compared with the control group, the ALT, AST, and TBIL levels of the treatment group changed, but the differences were not statistically significant (P > 0.05). Within-group comparisons showed that the ALT, AST, and TBIL levels of the observation group significantly decreased after treatment, with statistically significant differences (P 0.05). After treatment, compared with the control group, the scores for psychological, physiological, social-cultural, and environmental aspects of the treatment group increased significantly, with statistically significant differences (P Conclusion: The Jing ethnic group’s self-proposed formula combined with burning mugwort can improve the therapeutic effect and quality of life of patients after cholecystectomy for gallstones without causing adverse reactions.展开更多
This study aimed to investigate the effect of different gonadotropin-releasing hormone agonist (GnRH-a) administration methods on pregnancy outcomes of patients undergoing in-vitro fertilization-embryo transfer (IVF-E...This study aimed to investigate the effect of different gonadotropin-releasing hormone agonist (GnRH-a) administration methods on pregnancy outcomes of patients undergoing in-vitro fertilization-embryo transfer (IVF-ET). Clinical data of 5217 patients who underwent IVF-ET were retrospectively analyzed. Patients were divided into the long-acting GnRH-a group (n=1330) and the short-acting GnRH-a group (w=3887) based on their various treatment plans. The clinical and laboratory embryo data and clinical pregnancy outcomes were compared between the two groups. The results showed that there were no significant differences in the age, infertility, primary/secondary infertility rate, IVF rate, body mass index (BMI), antral follicle counting (AFC), folliclestimulating hormone (FSH) level, and the number of transplanted embryos between the two groups (P>0.05). There were no significant differences in the oocyte numbers, M II rate, fertilization rate, cleavage rate and blastocyst formation rate (P>0.05) between the two groups. The gonadotropin (Gn) using days, Gn dose and endometrial thickness were significantly greater in the long-acting GnRH-a group than those in the short-acting GnRH-a group (P<0.01). Additionally, the estradiol (E2) levels, blastocyst freezing rate, embryo utilization rate, transplant cancellation rate and abortion rate were significantly lower in the long-acting GnRH-a group than those in the shortacting GnRH-a group (P<0.01). The clinical pregnancy rate and embryo implantation rate were significantly higher in the long-acting GnRH-a group than in the short-acting GnRH-a group (P<O.Ol). It was concluded that use of long-acting GnRH-a can effectively reduce the transplant cancellation rate and improve the clinical pregnancy rate of the fresh cycle.展开更多
This study aims to compare the prevalence of sexually transmitted infections(STIs)with semen quality in men from couples with primary and secondary infertility.Semen samples were collected from 133 men who requested f...This study aims to compare the prevalence of sexually transmitted infections(STIs)with semen quality in men from couples with primary and secondary infertility.Semen samples were collected from 133 men who requested fertility evaluation.Seminal tract infection with Ureaplasma spp.(UU),Mycoplasma hominis(MH),Mycoplasma genitalium(MG),Chlamydia trachomatis(CT),Neisseria gonorrhoeae(NG),and herpes simplex virus-2(HSV-2)was assessed by PCR-based diagnostic assays.Among all patients,the prevalence of STIs was higher in men from couples with primary infertility than that in men from couples with secondary infertility(39.7%vs 21.7%,P=0.03).The prevalence of UU was 28.8%and 13.3%in men from couples with primary and secondary infertility,respectively.Men from couples with primary infertility were more likely to be positive for UU than men from couples with secondary infertility(P=0.04).Regarding the UU subtype,the prevalence of Ureaplasma urealyticum(Uuu)and Ureaplasma parvum(Uup;including Uup1,Uup3,Uup6,and Uup14)did not differ between the two groups.No associations between the prevalence rates of MH,MG,and CT were found in men from either infertility group.A lower sperm concentration was associated with STI pathogen positivity in men with primary infertility according to the crude model(P=0.04).The crude and adjusted models showed that semen volume(both P=0.03)and semen leukocyte count(both P=0.02)were independently associated with secondary infertility.These findings suggest the importance of classifying the type of infertility during routine diagnosis of seminal tract infections.展开更多
The objective of this study was to investigate variation and trends in dough rheological properties and flour quality traits in 330 Chinese wheat varieties. The dough rheological properties of development time(DT), st...The objective of this study was to investigate variation and trends in dough rheological properties and flour quality traits in 330 Chinese wheat varieties. The dough rheological properties of development time(DT), stability time(ST), and farinograph quality number(FQN)were evaluated, as well as the flour quality traits of protein(PC), wet gluten content(WGC), and sedimentation value(SV). The coefficients of variation of DT(40.5%), ST(58.1%), and FQN(42.4%) were higher than those of PC(9.1%), WGC(10.1%), and SV(15.3%). Normal distributions were observed for the flour quality indices but not for the rheological parameters. SV was strongly correlated with the three rheological parameters and accordingly might be used as a primary indicator for dough rheological property evaluation. Our results showed that there has been marked improvement in dough rheological properties for Chinese wheat varieties released since 1986, while flour quality has remained stable.展开更多
Kerogen plays an important role in shale gas adsorption,desorption and diffusion.Therefore,it is necessary to characterize the molecular structure of kerogen.In this study,four kerogen samples were isolated from the o...Kerogen plays an important role in shale gas adsorption,desorption and diffusion.Therefore,it is necessary to characterize the molecular structure of kerogen.In this study,four kerogen samples were isolated from the organic-rich shale of the Longmaxi Formation.Raman spectroscopy was used to determine the maturity of these kerogen samples.Highresolution transmission electron microscopy(HRTEM),13 C nuclear magnetic resonance(13 C NMR),X-ray diffraction(XRD)and Fourier transform infrared(FT-IR)spectroscopy were conducted to characterize the molecular structure of the shale samples.The results demonstrate that VReqv of these kerogen samples vary from 2.3%to 2.8%,suggesting that all the kerogen samples are in the dry gas window.The macromolecular carbon skeleton of the Longmaxi Formation kerogen is mainly aromatic(fa’=0.56).In addition,the aromatic structural units are mainly composed of naphthalene(23%),anthracene(23%)and phenanthrene(29%).However,the aliphatic structure of the kerogen macromolecules is relatively low(fal*+falH=0.08),which is presumed to be distributed in the form of methyl and short aliphatic chains at the edge of the aromatic units.The oxygen-containing functional groups in the macromolecules are mainly present in the form of carbonyl groups(fac=0.23)and hydroxyl groups or ether groups(falO=0.13).The crystallite structural parameters of kerogen,including the stacking height(Lc=22.84?),average lateral size(La=29.29?)and interlayer spacing(d002=3.43?),are close to the aromatic structural parameters of anthracite or overmature kerogen.High-resolution transmission electron microscopy reveals that the aromatic structure is well oriented,and more than 65%of the diffractive aromatic layers are concentrated in the main direction.Due to the continuous deep burial,the longer aliphatic chains and oxygen-containing functional groups in the kerogen are substantially depleted.However,the ductility and stacking degree of the aromatic structure increases during thermal evolution.This study provides quantitative information on the molecular structure of kerogen samples based on multiple research methods,which may contribute to an improved understanding of the organic pores in black shale.展开更多
The yield and nitrogen use efficiency(NUE)of hybrid rice combinations are closely related to restorer line.Therefore,it is essential to evaluate the agronomic characteristics of restorer lines with high yield and high...The yield and nitrogen use efficiency(NUE)of hybrid rice combinations are closely related to restorer line.Therefore,it is essential to evaluate the agronomic characteristics of restorer lines with high yield and high NUE(HYHN).However,it is unclear which restorer lines are HYHN,and neither have the common agronomic traits of the HYHN restorer lines been identified.Aiming to address this issue,we conducted two filed experiments using three nitrogen applications,which screened five HYHN restorer lines from 15 indica restorer lines.Yield,NUE and nutrient transportation of restorer lines with different yields and NUE types were examined.Yield and total nitrogen absorption in aboveground biomass(TNA)increased,whereas NUE for grain production decreased with increasing nitrogen application levels.The HYHN restorer lines had large spikelets and high weight per panicle that were significantly positively correlated with yield and NUE.Therefore,large sink potential may be beneficial for both yield and NUE.We further studied the differences in nutrient transportation to panicles between the HYHN and low yield and low NUE(LYLN)restorer lines and found that the former had a higher nitrogen absorption level and dry matter weight ratios of panicle in maturity.Moreover,the HYHN lines also had a higher root and neck-panicle node bleeding intensity per stem after heading and more developed vascular bundles of neck-panicle nodes and leaves than the LYLN lines,which could contribute to the transportation of nutrients from root to ground and from stem and leaf to spike.Therefore,the advantages of large sink potential of the HYHN restorer lines include large nutrient accumulation in and distribution to the panicles and smooth flow of nutrients along the transportation channels.展开更多
The shapes of particles and their distribution in tablets, controlled by pretreatment and tableting process, determine the pharmaceutical performance of excipient like lubricant. This study aims to provide deeper insi...The shapes of particles and their distribution in tablets, controlled by pretreatment and tableting process, determine the pharmaceutical performance of excipient like lubricant. This study aims to provide deeper insights to the relationship of the morphology and spatial distribution of stearic acid(SA) with the lubrication efficiency, as well as the resulting tablet property. Unmodified SA particles as flat sheet-like particles were firstly reprocessed by emulsification in hot water to obtain the reprocessed SA particles with spherical morphology. The three-dimensional(3 D) information of SA particles in tablets was detected by a quantitative and non-invasive 3 D structure elucidation technique, namely, synchrotron radiation X-ray micro-computed tomography(SR-μCT). SA particles in glipizide tablets prepared by using unmodified SA(GUT), reprocessed SA(GRT), as well as reference listed drug(RLD) of glipizide tablets were analyzed by SR-μCT. The results showed that the reprocessed SA with better flowability contributed to similarity of breaking forces between that of GRT and RLD. SA particles in GRT were very similar to those in RLD with uniform morphology and particle size, while SA particles in GUT were not evenly distributed. These findings not only demonstrated the feasibility of SR-μCT as a new method in revealing the morphology and spatial distribution of excipient in drug delivery system, but also deepened insights of solid dosage form design into a new scale by powder engineering.展开更多
Coupled nanogenerators have been a research hotspot due to their ability to harvest a variety of forms of energy such as light,mechanical and thermal energy and achieve a stable direct current output.Ferroelectric fil...Coupled nanogenerators have been a research hotspot due to their ability to harvest a variety of forms of energy such as light,mechanical and thermal energy and achieve a stable direct current output.Ferroelectric films are frequently investigated for photovoltaic applications due to their unique photovoltaic properties and bandgap-independent photovoltage,while the flexoelectric effect is an electromechanical property commonly found in solid dielectrics.Here,we effectively construct a new form of coupled nanogenerator based on a flexible BiFeO_(3) ferroelectric film that combines both flexoelectric and photovoltaic effects to successfully harvest both light and vibration energies.This device converts an alternating current into a direct current and achieves a 6.2% charge enhancement and a 19.3%energy enhancement to achieve a multi-dimensional"1+1>2"coupling enhancement in terms of current,charge and energy.This work proposes a new approach to the coupling of multiple energy harvesting mechanisms in ferroelectric nanogenerators and provides a new strategy to enhance the transduction efficiency of flexible functional devices.展开更多
The Qiwu Mine is located in the Ten Xian coal field of Shandong province.It experienced repeated volcanic activity,after the coal beds formed,where magma intrusion was significant The effect of coal reservoir porosity...The Qiwu Mine is located in the Ten Xian coal field of Shandong province.It experienced repeated volcanic activity,after the coal beds formed,where magma intrusion was significant The effect of coal reservoir porosity after magma intrusion was studied by analysis of regional and mine structure and magmatic activity.Experimental methods including maceral measurement under the microscope and mercury porosimetry were used for testing the pore structure.The authors believe that magma intrusion into low-rank bituminous coal causes reservoir porosity to gradually increase：the closer to the magmatic rock a sample is,the less the porosity.The pore size distribution also changes.In the natural coal bed the pore size is mainly in the transitive and middle pore range.However,the coal changes to anthracite next to the magmatic rock and larger pores dominate.Regional magma thermal evolution caused coal close to magmatic rock to be roasted,which reduced the volatile matter,developed larger holes,and destroyed plant tissue holes.The primary reason for a porosity decrease in the vicinity of magmatic rock is that Bituminite resulting from the roasting fills the holes that were present initially.展开更多
基金support from the“111 program”of Ministry of Education of China and State Administration of Foreign Experts Affairs of China.
文摘BACKGROUND Uterine injury can cause uterine scarring,leading to a series of complications that threaten women’s health.Uterine healing is a complex process,and there are currently no effective treatments.Although our previous studies have shown that bone marrow mesenchymal stem cells(BMSCs)promote uterine damage repair,the underlying mechanisms remain unclear.However,exploring the specific regulatory roles of BMSCs in uterine injury treatment is crucial for further understanding their functions and enhancing therapeutic efficacy.AIM To investigate the underlying mechanism by which BMSCs promote the process of uterine healing.METHODS In in vivo experiments,we established a model of full-thickness uterine injury and injected BMSCs into the uterine wound.Transcriptome sequencing was per-formed to determine the enrichment of differentially expressed genes at the wound site.In in vitro experiments,we isolated rat uterine smooth muscle cells(USMCs)and cocultured them with BMSCs to observe the interaction between BMSCs and USMCs in the microenvironment.RESULTS We found that the differentially expressed genes were mainly related to cell growth,tissue repair,and angiogenesis,while the phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)pathway was highly enriched.Quantitative reverse-transcription polymerase chain reaction was used to validate differentially expressed genes,and the results demonstrated that BMSCs can upregulate genes related to regeneration and downregulate genes related to inflammation.Coculturing BMSCs promoted the migration and proliferation of USMCs,and the USMC microenvironment promoted the myogenic differentiation of BMSCs.Finally,we validated the PI3K/AKT pathway in tissues and cells and showed that BMSCs activate the PI3K/AKT pathway to promote the regeneration of uterine smooth muscle both in vivo and in vitro.CONCLUSION BMSCs upregulated uterine wound regeneration and anti-inflammatory factors and enhanced uterine smooth muscle proliferation through the PI3K/AKT pathway both in vivo and in vitro.
基金supported by the National Natural Science Foundation of China(Nos.41807264,41972289)the Engineering Research Center of Rock-Soil Drilling&Excavation and Protection,Ministry of Education(No.202102)+3 种基金the Key Laboratory of Geological Hazards on Three Gorges Reservoir Area(China Three Gorges University),Ministry of Education(No.2020KDZ01)the Fundamental Research Funds for the Central Universities,China University of Geosciences(Wuhan)(Nos.CUG170686,CUGQY1932)the China Scholarship Council(No.201406410032)the Science and Technology Research Project of Education Department of Hubei Province(Nos.B2019452,B2024509)。
文摘A DFN-DEC(discrete fracture network-distinct element code)method based on the MATLAB platform is developed to generate heterogeneous DFN.Subsequently,the effects of the spatial variability(the meanμand the standard deviationσ)of the geometric properties(i.e.,the fracture dip D,the trace length T and the spacing S)of both the gently-dipping(denoted with 1)and the steeply-dipping(denoted with 2)fractures on the stability of granite slope are investigated.Results indicate that the proposed DFN-DEC method is robust,generating fracture networks that resemble reality.In addition,the spatial variability of fracture geometry,influencing the structure of granite slope,plays a significant role in slope stability.The mean stability of the slope decreases with the increase ofμ_(D_(1))(the mean of gently-dipping fracture dip),σ_(D_(2))(the mean of steeply-dipping fracture dip),μ_(T_(1))(the mean of gently-dipping fracture trace length),μ_(T_(2))(the mean of steeply-dipping fracture trace length),σ_(T_(1))(the standard deviation of gently-dipping fracture trace length),σ_(T_(2))(the standard deviation of steeply-dipping fracture trace length),and the decrease ofσ_(D_(1))(the standard deviation of gently-dipping fracture dip),μ_(D_(2))(the standard deviation of steeply-dipping fracture dip),μ_(S_(1))(the mean of gently-dipping fracture spacing)andμ_(S_(2))(the mean of steeply-dipping fracture spacing).Among them,μ_(T_(1)),μ_(D_(1))andμ_(S_(1))have the major impact.When the fracture spacing is large,the variability in the fracture geometry becomes less relevant to slope stability.When within some ranges of the fracture spacing,the spatial varying of dips can increase the slope stability by forming an interlaced structure.The results also show that the effects of the variability of trace length on slope stability depend on the variability of dip.These findings highlight the importance of spatial variability in the geometry of fractures to rock slope stability analysis.
基金Supported by National Natural Science Foundation of China,No.82300694 and No.81970523Natural Science Foundation of Hunan Province,No.2022JJ70165,No.2021JJ31067,No.2023JJ40828 and No.2022JJ40704.
文摘BACKGROUND Hepatocellular carcinoma(HCC)is a significant global health challenge with rising incidence rates and poor prognoses.Aminoacyl-tRNA synthetases(ARSs)are important regulators implicated in the occurrence and progression of several cancers.However,their specific function in HCC remains unclear,and ARSs-related prognostic factors for patient stratification are lacking.AIM To investigate the ARSs-related mechanisms of HCC and establish an effective prognostic risk model for stratifying patients with HCC.METHODS We screened ARSs genes of interest using differential gene expression,mutation,and survival analysis.Western blot and Immunohistochemistry were used to analyze MARS1 expression in the liver tissues of patients with HCC.Functional studies,including CCK-8 cell viability assay,EdU cell proliferation assay,cell cycle assays,Transwell migration and invasion assays,and in vivo tumor xenograft models,were conducted to comprehensively elucidate the specific role of MARS1 in HCC.Moreover,the MARS1-related prognostic score(MRPS)was established by LASSO regression and Cox regression analysis in The Cancer Genome Atlas-HCC and GSE14520 cohorts.Patients’immunotherapy and chemotherapy responses were predicted by immunomicroenvironment and drug susceptibility analysis in both subgroups.RESULTS MARS1 was selected as a target gene from a series of ARSs genes,with markedly higher expression observed in HCC tissues compared to adjacent non-cancerous tissues.Silencing MARS1 considerably impeded the proliferation,migration,invasion,and tumorigenic abilities of HCC cells in vitro and in vivo.Moreover,high MRPSs were associated with poor overall survival,altered infiltration of T cells,macrophages,monocytes,elevated immune checkpoint expression(PD-L1,CTLA4,LAG3),and reduced drug sensitivity in HCC.CONCLUSION MARS1 promotes HCC development and represents a potential therapeutic target for HCC management.Furthermore,MRPS serves as an independent prognostic factor for survival and a predictor of tumor treatment response.
基金Supported by Noncommunicable Chronic Diseases-National Science and Technology Major Project,No.2023ZD0507200The Suqian Sci Tech Program,No.Z2023106+2 种基金National Natural Science Foundation of China,No.82071234,No.31400947 and No.82170836Gusu Talent Program,No.GSWS2022030The Clinical Research Center of Neurological Disease of The Second Affiliated Hospital of Soochow University,No.ND2024A02.
文摘BACKGROUND Diabetic gastrointestinal autonomic neuropathy(DGAN)often affects the patients’quality of life.Thus,research on influencing factors of DGAN may promote DGAN prevention and decrease the incidence rate.This study used electrogastrogram(EGG)to assess the relationship between metabolic dysfunction-associated steatotic liver disease(MASLD)and DGAN.AIM To analyze the changes of EGG in patients with MASLD and type 2 diabetes mellitus(T2DM),and to elucidate whether ultrasound-diagnosed MASLD is an independent risk factor for diabetic gastric motility disorders(DGMD).METHODS A total of 272 patients with T2DM hospitalized at the Second Affiliated Hospital of Soochow University from December 2020 to December 2021 and the Affiliated Suqian Hospital of Xuzhou Medical University from November 2023 to June 2024 were included in the cross-sectional study.General information,clinical data,and medical history of all study subjects,including name,age,gender,body mass index,and duration of diabetes were collected.Laboratory tests included biochemical parameters,glycosylated hemoglobin(HbA1c),fasting C-peptide,2h postprandial C-peptide and 25-hydroxyvitamin D[25(OH)D].EGG,fundus examination and carotid artery ultrasonography were performed and results were recorded.According to the results of EGG,the subjects were divided into the DGMD group and non-DGMD group.RESULTS The duration of diabetes,fasting blood glucose(FBG),HbA1c,25(OH)D and the prevalence of MASLD were significantly higher in the DGMD group(P<0.05).Multiple logistic regression analysis showed that the duration,FBG,25(OH)D and the presence of MASLD were independent influencing factors.CONCLUSION MASLD is strongly associated with an increased incidence of DGMD.Timely treatment of MASLD is effective to prevent diabetic gastroparesis.
基金supported by National Natural Science Foundation of China(42002293,52068019)Hainan Provincial Natural Science Foundation of China(520QN229,422RC599)+2 种基金Independent Innovation Fund Project of Tianjin University and Hainan University(KF2022⁃03)Scientific Research Startup Foundation of Hainan university(KYQD(2R)1969)Systematic Project of Key Laboratory of Geological Hazards on Three Gorges Reservoir Area(Three Gorges University),Ministry of Education(2020KDZ04).
文摘The chief objective of the article is to learn the spatial characteristics of stress distribution around a shallow buried cylinder Karst cave in limestone strata.Firstly,taking into account the geometry of limestone formations,and the characteristics of Karst geomorphology in China,a spatial axialsymmetrical hollow model was established.Concurrently,combining available work and the concept of elasticity,the boundary conditions are determined.Subsequently,Love displacement method was introduced,the expressions of stress components were gained.The diagram characteristics of each stress component are summarized,which are affected by various influencing factors.Finally,in order to prove the rationality of the general solution,numerical simulation was carried out on the basis of practical engineering,and the maximum error is less than 5%.Thus,the analytical solution could represent the spatial characteristics of stress distribution around a shallow buried cylinder Karst cave in limestone strata.
文摘Jing Medicine, as an important part of China’s ethnic medicine and culture, is characterized by its unique theories, rich content, and abundant resources, showcasing distinctive features of minority regions. The Jing ethnic group is distributed in coastal and border areas of China, and after thousands of years of development, it has formed a rich marine-oriented medical system in the struggle against the environment and diseases, displaying unique medication characteristics and clinical efficacy. According to Jing Medicine, the human body is an organic whole that is unified with the natural and social environment. It believes that the heavens and earth are the spirits of all things, and all things possess spiritual energy, which is closely connected to the human body’s spiritual energy. The utilization of marine organisms in medicine is the greatest feature of Jing Medicine, and it is a common approach used by the local population for disease prevention and treatment. To further explore Jing Medicine, improve the repository of ethnic medicine culture, and address the insufficient understanding of Jing Medicine in traditional Chinese medicine culture, this article provides a comprehensive review of the distinctive diagnostic and therapeutic theories, techniques, and applications of internal and external treatments in Jing Medicine.
文摘Objective: To observe the clinical efficacy of a Jing ethnic prescription in treating chronic atrophic gastritis (CAG). Methods: A total of 182 CAG patients admitted to our hospital were randomly divided into a control group (91 cases) and a treatment group (91 cases) based on their admission order. The control group received conventional treatment, while the treatment group was treated with a Jing ethnic prescription. The clinical efficacy, changes in gastric mucosa-related indicators, and systemic inflammatory markers were compared between the two groups. Results: After treatment, the overall effective rate in the treatment group was higher than that in the control group, but the difference was not statistically significant (P > 0.05). The levels of trefoil factor 2 (TFF2) in the gastric mucosa increased, and the levels of nuclear factor-kappa B (NF-κB) decreased in both groups. However, the improvement in these indicators was significantly better in the treatment group (P Conclusion: The custom formula of the Jing ethnic group shows comparable clinical efficacy to conventional treatment for chronic atrophic gastritis (CAG), but it demonstrates significantly better effects in reducing systemic inflammatory responses. Specifically, the treatment group showed superior results in the following aspects compared to the control group: increased levels of TFF2, decreased levels of NF-κB, and reduced serum levels of IL-6, IL-8, and hs-CRP.
文摘Objective: To explore the clinical efficacy of the Jing ethnic group’s self-proposed formula combined with burning mugwort on patients after cholecystectomy for gallstones. Methods: Sixty-four patients with gallstones who received inpatient treatment at the Hepatobiliary Surgery Department of Fangchenggang Traditional Chinese Medicine Hospital from November 2022 to November 2023 were selected and randomly divided into a control group and an observation group. The treatment group was treated with the Jing ethnic group’s self-proposed formula combined with burning mugwort, while the control group was treated with ursodeoxycholic acid tablets. The liver function indicators, therapeutic effect, safety evaluation, and quality of life of the two groups were compared. Results: After treatment, compared with the control group, the ALT, AST, and TBIL levels of the treatment group changed, but the differences were not statistically significant (P > 0.05). Within-group comparisons showed that the ALT, AST, and TBIL levels of the observation group significantly decreased after treatment, with statistically significant differences (P 0.05). After treatment, compared with the control group, the scores for psychological, physiological, social-cultural, and environmental aspects of the treatment group increased significantly, with statistically significant differences (P Conclusion: The Jing ethnic group’s self-proposed formula combined with burning mugwort can improve the therapeutic effect and quality of life of patients after cholecystectomy for gallstones without causing adverse reactions.
基金This work was supported by the Natural Science Foundation of Hubei Province (No.2017CFB262).
文摘This study aimed to investigate the effect of different gonadotropin-releasing hormone agonist (GnRH-a) administration methods on pregnancy outcomes of patients undergoing in-vitro fertilization-embryo transfer (IVF-ET). Clinical data of 5217 patients who underwent IVF-ET were retrospectively analyzed. Patients were divided into the long-acting GnRH-a group (n=1330) and the short-acting GnRH-a group (w=3887) based on their various treatment plans. The clinical and laboratory embryo data and clinical pregnancy outcomes were compared between the two groups. The results showed that there were no significant differences in the age, infertility, primary/secondary infertility rate, IVF rate, body mass index (BMI), antral follicle counting (AFC), folliclestimulating hormone (FSH) level, and the number of transplanted embryos between the two groups (P>0.05). There were no significant differences in the oocyte numbers, M II rate, fertilization rate, cleavage rate and blastocyst formation rate (P>0.05) between the two groups. The gonadotropin (Gn) using days, Gn dose and endometrial thickness were significantly greater in the long-acting GnRH-a group than those in the short-acting GnRH-a group (P<0.01). Additionally, the estradiol (E2) levels, blastocyst freezing rate, embryo utilization rate, transplant cancellation rate and abortion rate were significantly lower in the long-acting GnRH-a group than those in the shortacting GnRH-a group (P<0.01). The clinical pregnancy rate and embryo implantation rate were significantly higher in the long-acting GnRH-a group than in the short-acting GnRH-a group (P<O.Ol). It was concluded that use of long-acting GnRH-a can effectively reduce the transplant cancellation rate and improve the clinical pregnancy rate of the fresh cycle.
基金supported by the National Key Research and Development Project(2019YFA0802600)the National Natural Science Foundation of China(No.81901543,81901545,81971333,82001495,32002354)+2 种基金the Fundamental Research Funds for the Central Universities(WK9110000063)the State Key Laboratory of Reproductive Medicine(SKLRM-K201904)the Natural Science Foundation of Anhui Provincial of China(1908085QH315).
文摘This study aims to compare the prevalence of sexually transmitted infections(STIs)with semen quality in men from couples with primary and secondary infertility.Semen samples were collected from 133 men who requested fertility evaluation.Seminal tract infection with Ureaplasma spp.(UU),Mycoplasma hominis(MH),Mycoplasma genitalium(MG),Chlamydia trachomatis(CT),Neisseria gonorrhoeae(NG),and herpes simplex virus-2(HSV-2)was assessed by PCR-based diagnostic assays.Among all patients,the prevalence of STIs was higher in men from couples with primary infertility than that in men from couples with secondary infertility(39.7%vs 21.7%,P=0.03).The prevalence of UU was 28.8%and 13.3%in men from couples with primary and secondary infertility,respectively.Men from couples with primary infertility were more likely to be positive for UU than men from couples with secondary infertility(P=0.04).Regarding the UU subtype,the prevalence of Ureaplasma urealyticum(Uuu)and Ureaplasma parvum(Uup;including Uup1,Uup3,Uup6,and Uup14)did not differ between the two groups.No associations between the prevalence rates of MH,MG,and CT were found in men from either infertility group.A lower sperm concentration was associated with STI pathogen positivity in men with primary infertility according to the crude model(P=0.04).The crude and adjusted models showed that semen volume(both P=0.03)and semen leukocyte count(both P=0.02)were independently associated with secondary infertility.These findings suggest the importance of classifying the type of infertility during routine diagnosis of seminal tract infections.
基金supported by the Science and Technology Innovation Project of CAAS for Wang Tianyu (Crop Germplasm Resources Identification and Discovery)
文摘The objective of this study was to investigate variation and trends in dough rheological properties and flour quality traits in 330 Chinese wheat varieties. The dough rheological properties of development time(DT), stability time(ST), and farinograph quality number(FQN)were evaluated, as well as the flour quality traits of protein(PC), wet gluten content(WGC), and sedimentation value(SV). The coefficients of variation of DT(40.5%), ST(58.1%), and FQN(42.4%) were higher than those of PC(9.1%), WGC(10.1%), and SV(15.3%). Normal distributions were observed for the flour quality indices but not for the rheological parameters. SV was strongly correlated with the three rheological parameters and accordingly might be used as a primary indicator for dough rheological property evaluation. Our results showed that there has been marked improvement in dough rheological properties for Chinese wheat varieties released since 1986, while flour quality has remained stable.
基金supported by the National Science and Technology Major Project(2017ZX05035004–002)the National Natural Science Foundation of China(no.41702167)+1 种基金the Fundamental Research Funds for the Central Universities(No.2017CXNL03)the Scientific Research Foundation of the Key Laboratory of Coalbed Methane Resources and Reservoir Formation Process,Ministry of Education(China University of Mining and Technology)(No.2017–007)
文摘Kerogen plays an important role in shale gas adsorption,desorption and diffusion.Therefore,it is necessary to characterize the molecular structure of kerogen.In this study,four kerogen samples were isolated from the organic-rich shale of the Longmaxi Formation.Raman spectroscopy was used to determine the maturity of these kerogen samples.Highresolution transmission electron microscopy(HRTEM),13 C nuclear magnetic resonance(13 C NMR),X-ray diffraction(XRD)and Fourier transform infrared(FT-IR)spectroscopy were conducted to characterize the molecular structure of the shale samples.The results demonstrate that VReqv of these kerogen samples vary from 2.3%to 2.8%,suggesting that all the kerogen samples are in the dry gas window.The macromolecular carbon skeleton of the Longmaxi Formation kerogen is mainly aromatic(fa’=0.56).In addition,the aromatic structural units are mainly composed of naphthalene(23%),anthracene(23%)and phenanthrene(29%).However,the aliphatic structure of the kerogen macromolecules is relatively low(fal*+falH=0.08),which is presumed to be distributed in the form of methyl and short aliphatic chains at the edge of the aromatic units.The oxygen-containing functional groups in the macromolecules are mainly present in the form of carbonyl groups(fac=0.23)and hydroxyl groups or ether groups(falO=0.13).The crystallite structural parameters of kerogen,including the stacking height(Lc=22.84?),average lateral size(La=29.29?)and interlayer spacing(d002=3.43?),are close to the aromatic structural parameters of anthracite or overmature kerogen.High-resolution transmission electron microscopy reveals that the aromatic structure is well oriented,and more than 65%of the diffractive aromatic layers are concentrated in the main direction.Due to the continuous deep burial,the longer aliphatic chains and oxygen-containing functional groups in the kerogen are substantially depleted.However,the ductility and stacking degree of the aromatic structure increases during thermal evolution.This study provides quantitative information on the molecular structure of kerogen samples based on multiple research methods,which may contribute to an improved understanding of the organic pores in black shale.
基金the National Natural Science Foundation of China(31871564)the National Key R&D Program of China(2016YFD0300506 and 2017YFD0301700)the Sichuan Breeding Key Research Project,China(2016NYZ0051)。
文摘The yield and nitrogen use efficiency(NUE)of hybrid rice combinations are closely related to restorer line.Therefore,it is essential to evaluate the agronomic characteristics of restorer lines with high yield and high NUE(HYHN).However,it is unclear which restorer lines are HYHN,and neither have the common agronomic traits of the HYHN restorer lines been identified.Aiming to address this issue,we conducted two filed experiments using three nitrogen applications,which screened five HYHN restorer lines from 15 indica restorer lines.Yield,NUE and nutrient transportation of restorer lines with different yields and NUE types were examined.Yield and total nitrogen absorption in aboveground biomass(TNA)increased,whereas NUE for grain production decreased with increasing nitrogen application levels.The HYHN restorer lines had large spikelets and high weight per panicle that were significantly positively correlated with yield and NUE.Therefore,large sink potential may be beneficial for both yield and NUE.We further studied the differences in nutrient transportation to panicles between the HYHN and low yield and low NUE(LYLN)restorer lines and found that the former had a higher nitrogen absorption level and dry matter weight ratios of panicle in maturity.Moreover,the HYHN lines also had a higher root and neck-panicle node bleeding intensity per stem after heading and more developed vascular bundles of neck-panicle nodes and leaves than the LYLN lines,which could contribute to the transportation of nutrients from root to ground and from stem and leaf to spike.Therefore,the advantages of large sink potential of the HYHN restorer lines include large nutrient accumulation in and distribution to the panicles and smooth flow of nutrients along the transportation channels.
基金The authors are grateful for the financial support from the National Science and Technology Major Project(2017ZX09101001-006)Thanks to the BL13W1 beamline of the SSRF for the precious beam time and help from the team.
文摘The shapes of particles and their distribution in tablets, controlled by pretreatment and tableting process, determine the pharmaceutical performance of excipient like lubricant. This study aims to provide deeper insights to the relationship of the morphology and spatial distribution of stearic acid(SA) with the lubrication efficiency, as well as the resulting tablet property. Unmodified SA particles as flat sheet-like particles were firstly reprocessed by emulsification in hot water to obtain the reprocessed SA particles with spherical morphology. The three-dimensional(3 D) information of SA particles in tablets was detected by a quantitative and non-invasive 3 D structure elucidation technique, namely, synchrotron radiation X-ray micro-computed tomography(SR-μCT). SA particles in glipizide tablets prepared by using unmodified SA(GUT), reprocessed SA(GRT), as well as reference listed drug(RLD) of glipizide tablets were analyzed by SR-μCT. The results showed that the reprocessed SA with better flowability contributed to similarity of breaking forces between that of GRT and RLD. SA particles in GRT were very similar to those in RLD with uniform morphology and particle size, while SA particles in GUT were not evenly distributed. These findings not only demonstrated the feasibility of SR-μCT as a new method in revealing the morphology and spatial distribution of excipient in drug delivery system, but also deepened insights of solid dosage form design into a new scale by powder engineering.
基金This work was supported by the National Natural Science Foundation of China(No.52072041)the Beijing Natural Science Foundation(No.JQ21007)the University of Chinese Academy of Sciences(No.Y8540XX2D2).
文摘Coupled nanogenerators have been a research hotspot due to their ability to harvest a variety of forms of energy such as light,mechanical and thermal energy and achieve a stable direct current output.Ferroelectric films are frequently investigated for photovoltaic applications due to their unique photovoltaic properties and bandgap-independent photovoltage,while the flexoelectric effect is an electromechanical property commonly found in solid dielectrics.Here,we effectively construct a new form of coupled nanogenerator based on a flexible BiFeO_(3) ferroelectric film that combines both flexoelectric and photovoltaic effects to successfully harvest both light and vibration energies.This device converts an alternating current into a direct current and achieves a 6.2% charge enhancement and a 19.3%energy enhancement to achieve a multi-dimensional"1+1>2"coupling enhancement in terms of current,charge and energy.This work proposes a new approach to the coupling of multiple energy harvesting mechanisms in ferroelectric nanogenerators and provides a new strategy to enhance the transduction efficiency of flexible functional devices.
基金the National Basic Research Program of China(No.2009CB219605)the Key Program of the National Natural Science Foundation of China(No.40730422)the National Major Project of Science and Technology(No.2008ZX05034-04)
文摘The Qiwu Mine is located in the Ten Xian coal field of Shandong province.It experienced repeated volcanic activity,after the coal beds formed,where magma intrusion was significant The effect of coal reservoir porosity after magma intrusion was studied by analysis of regional and mine structure and magmatic activity.Experimental methods including maceral measurement under the microscope and mercury porosimetry were used for testing the pore structure.The authors believe that magma intrusion into low-rank bituminous coal causes reservoir porosity to gradually increase：the closer to the magmatic rock a sample is,the less the porosity.The pore size distribution also changes.In the natural coal bed the pore size is mainly in the transitive and middle pore range.However,the coal changes to anthracite next to the magmatic rock and larger pores dominate.Regional magma thermal evolution caused coal close to magmatic rock to be roasted,which reduced the volatile matter,developed larger holes,and destroyed plant tissue holes.The primary reason for a porosity decrease in the vicinity of magmatic rock is that Bituminite resulting from the roasting fills the holes that were present initially.
