Alzheimer's disease is an age-related neurodegenerative disorder with a complex and incompletely understood pathogenesis. Despite extensive research, a cure for Alzheimer's disease has not yet been found. Oxid...Alzheimer's disease is an age-related neurodegenerative disorder with a complex and incompletely understood pathogenesis. Despite extensive research, a cure for Alzheimer's disease has not yet been found. Oxidative stress mediates excessive oxidative responses, and its involvement in Alzheimer's disease pathogenesis as a primary or secondary pathological event is widely accepted. As a member of the selenium-containing antioxidant enzyme family, glutathione peroxidase 4 reduces esterified phospholipid hydroperoxides to maintain cellular redox homeostasis. With the discovery of ferroptosis, the central role of glutathione peroxidase 4 in anti-lipid peroxidation in several diseases, including Alzheimer's disease, has received widespread attention. Increasing evidence suggests that glutathione peroxidase 4 expression is inhibited in the Alzheimer's disease brain, resulting in oxidative stress, inflammation, ferroptosis, and apoptosis, which are closely associated with pathological damage in Alzheimer's disease. Several therapeutic approaches, such as small molecule drugs, natural plant products, and non-pharmacological treatments, ameliorate pathological damage and cognitive function in Alzheimer's disease by promoting glutathione peroxidase 4 expression and enhancing glutathione peroxidase 4 activity. Therefore, glutathione peroxidase 4 upregulation may be a promising strategy for the treatment of Alzheimer's disease. This review provides an overview of the gene structure, biological functions, and regulatory mechanisms of glutathione peroxidase 4, a discussion on the important role of glutathione peroxidase 4 in pathological events closely related to Alzheimer's disease, and a summary of the advances in small-molecule drugs, natural plant products, and non-pharmacological therapies targeting glutathione peroxidase 4 for the treatment of Alzheimer's disease. Most prior studies on this subject used animal models, and relevant clinical studies are lacking. Future clinical trials are required to validate the therapeutic effects of strategies targeting glutathione peroxidase 4 in the treatment of Alzheimer's disease.展开更多
INTRODUCTION.On January 7,2025,at 9:05 AM BJT,a MS6.8 earthquake(CENC epicenter:28.50°N,87.45°E)struck Dingri County,Xizang Province(hereinafter referred to as the Dingri mainshock).The inferred moment magni...INTRODUCTION.On January 7,2025,at 9:05 AM BJT,a MS6.8 earthquake(CENC epicenter:28.50°N,87.45°E)struck Dingri County,Xizang Province(hereinafter referred to as the Dingri mainshock).The inferred moment magnitude,based on regional/teleseismic waveform inversion and back-projection,is approximately MW7.1.Focal mechanism solutions,aftershock distribution,and field surveys indicate that the Dingri mainshock was a normal-faulting event,with a nearly north-south strike and a westward-dipping fault plane.展开更多
Acute myocardial infarction(AMI)is a leading cause of death and disability worldwide and consists of two distinct cardiac pathologies:ST-elevation myocardial infarction(STEMI)and non-ST-elevation myocardial infarction...Acute myocardial infarction(AMI)is a leading cause of death and disability worldwide and consists of two distinct cardiac pathologies:ST-elevation myocardial infarction(STEMI)and non-ST-elevation myocardial infarction(NSTEMI).[1]In China,AMI is widely recognized as a predominant cause of mortality in both urban and rural demographics,based on the 2022 Report on Cardiovascular Health and Diseases in China;moreover,its mortality rate has been reported to be rising,with a recurrence rate of 2.5%within one year.展开更多
bHLH transcription factors,widely exist in various plants,and are vital for the growth and development of these plants.Among them,many have been implicated in anthocyanin biosynthesis across various plants.In the pres...bHLH transcription factors,widely exist in various plants,and are vital for the growth and development of these plants.Among them,many have been implicated in anthocyanin biosynthesis across various plants.In the present study,a PdbHLH57 gene,belonging to the bHLH IIIf group,was characterized,which was isolated and cloned from the colored-leaf poplar‘Zhongshancaiyun’(ZSCY).The cDNA sequence of PdbHLH57 was 1887 base pairs,and the protein encoded by PdbHLH57 had 628 amino acids,the isoelectric point and molecular weight of which were 6.26 and 69.75 kDa,respectively.Through bioinformatics analysis,PdbHLH57 has been classified into the IIIf bHLH subgroup,with many members of this subgroup known to participate in anthocyanin biosynthesis.The subcellular localization analysis conducted in the leaf protoplasts of‘ZSCY’revealed that the PdbHLH57 protein is specifically localized in the nucleus.The transcription activation analysis was also conducted,and the results showed that the PdbHLH57 protein had self-transcription activation.To better explore the functions of the PdbHLH57 protein,two parts of this protein(PdbHLH57-1,PdbHLH57-2)were split to detect their transcriptional activation activity.The results indicated that PdbHLH57-1(1-433aa)had self-transcription activation,and PdbHLH57-2(433-628aa)had no transcription activation.The expression of PdbHLH57 peaked in June during different developmental stages in‘ZSCY’,and it was most highly expressed in the phloem among various tissues.These findings offer a basis for understanding the role of PdbHLH57 in colored-leaf poplar.展开更多
Due to insufficient energy density,supercapacitors(SCs)with preeminent-power and long cycle stability cannot be implemented in some practical applications.Exploring hybrid materials with redox activity to emerge high ...Due to insufficient energy density,supercapacitors(SCs)with preeminent-power and long cycle stability cannot be implemented in some practical applications.Exploring hybrid materials with redox activity to emerge high specific capacitance in ionic liquid(IL)electrolytes can solve this problem.Herein,we report a redox-organic molecule 2,6-diaminoanthraquinone(DAAQ)modified MXene(Ti3C2Tx)/Graphene(DAAQ-M/G)composite material.With the assist of graphene oxide(GO),MXene and graphene fabricate a three-dimensional(3D)interconnected structure as a conductive framework,which inhibits self-stacking of MXene monolayers and ensures high electronic conductivity.Meanwhile,DAAQ is loaded onto the M/G framework through covalent/non-covalent functionalization.The DAAQ as a spacer effectively enlarges the interlayer spacing of MXene nanosheets,and meanwhile produces reversible redox reactions during charge/discharge processes to provide additional Faradaic contribution to capacity.Therefore,the specific capacitance(capacity)of the DAAQ-M/G as the negative electrode material reaches to 226 F g^(-1)(306 C g^(-1))at 1 A g^(-1)in 1-ethyl-3-methylimidazolium tetrafluoroborate(EmimBF4)electrolyte.Furthermore,an asymmetric supercapacitor(ASC)is assembled using DAAQ-M/G as the negative electrode and self-prepared organic molecule hydroquinone modified reduced graphene oxide(HQ-RGO)material as the positive electrode,with a high energy density of 43 Wh kg^(-1)at high power density of 1669 Wkg^(-1).The ASC can maintain 80%of initial specific capacitance after 9000 cycles.This research can provide better support to develop advanced organic molecules-modified MXene composite materials for ionic liquid-based SCs.展开更多
Electron-positron colliders operating in the GeV center-of-mass range,or tau-charm energy region,have been proved to enable competitive frontier research due to several unique features.With the progress of high-energy...Electron-positron colliders operating in the GeV center-of-mass range,or tau-charm energy region,have been proved to enable competitive frontier research due to several unique features.With the progress of high-energy physics in the last two decades,a new-generation Tau-Charm factory,called the Super Tau-Charm Facility(STCF),has been actively promoted by the particle physics community in China.STCF has the potential to address fundamental questions such as the essence of color confinement and the matter-antimatter asymmetry within the next decades.The main design goals of the STCF are a center-of-mass energy ranging from 2 to 7 GeV and a luminosity surpassing 5×10^(34)cm^(−2)s^(−1)that is optimized at a center-of-mass energy of 4 GeV,which is approximately 50 times that of the currently operating Tau-Charm factory-BEPCII.The STCF accelerator has two main parts:a double-ring collider with a crab-waist collision scheme and an injector that provides top-up injections for both electron and positron beams.As a typical third-generation electron-positron circular collider,the STCF accelerator faces many challenges in both accelerator physics and technology.In this paper,the conceptual design of the STCF accelerator complex is presented,including the ongoing efforts and plans for technological research and develop-ment,as well as the required infrastructure.The STCF project aims to secure support from the Chinese central government for its construction during the 15th Five-Year Plan(2026-2030).展开更多
At 20:08 on September 18,2024,Beijing time,an earthquake of magnitude 4.7 occurred in Feidong,Anhui Province(31.98°N,117.6°E).The China Earthquake Early Warning Network presented the first early warning resu...At 20:08 on September 18,2024,Beijing time,an earthquake of magnitude 4.7 occurred in Feidong,Anhui Province(31.98°N,117.6°E).The China Earthquake Early Warning Network presented the first early warning results 8.9 s after the earthquake.The China Earthquake Networks Center(CENC)released the automatic rapid report results 163 s after the earthquake and the official rapid report results 8 min after the earthquake.At the same time,the CENC reported a series of emergency products,including source parameters,seismic tectonics,historical earthquakes,focal mechanism,instrument seismic intensity and predicted intensity.The results showed that the earthquake was located at the junction of the southern section of the Tanlu Fault and the Feizhong Fault,with aftershocks distributed in the NEE direction.The focal mechanism solution indicates that the earthquake is essentially a strike-slip event.The predicted intensity in the vicinity of the epicenter reaches up to VI,involving 23towns that cover an area of about 1359 km^(2).Only one station near the epicenter shows a peak acceleration value greater than the fortification standard of the area,which may cause slight damage to some adjacent houses,consistent with the actual damage to buildings.展开更多
From the perspective of village classification, a set of methods for accurately measuring the potential of rural settlement consolidation are proposed. Taking Feicheng in Shandong Province as the research area, combin...From the perspective of village classification, a set of methods for accurately measuring the potential of rural settlement consolidation are proposed. Taking Feicheng in Shandong Province as the research area, combined with the corresponding planning, a classification and evaluation system for rural residential areas was constructed to classify rural residential areas from the four levels of natural resources, economy and society, supporting facilities and livability. The theoretical and practical potential of one type of rural settlements (relocating and merger village) is mainly calculated, and the potential scale, potential level and potential spatial distribution of different regions are analyzed. (Level and spatial distribution are analyzed. The results showed that the 621 rural settlements can be divided into five types: 148 urban-rural integration villages (URIV), 41 historical and cultural villages (HCV), 56 cluster developing villages (CDV), 155 keeping and limiting villages (KLV) and 221 relocating and merger villages (RMV). According to calculations, the theoretical potential of relocating and merger village areas was 1971.47 hm2, accounting for 19.52% of the total scale of Feicheng residential areas;the comprehensive correction coefficient of each region was 0.48 - 0.70, and the revised actual potential is 1082.68 hm2, accounting for the theoretical potential 54.92% of the total. There were large spatial differences in the scale of potential in different regions. In general, the theoretical and practical potential of the central and southern regions was higher than that of the northern regions.展开更多
Hyperspectral unmixing is a powerful tool for the remote sensing image mining. Nonnegative matrix factorization (NMF) has been adopted to deal with this issue, while the precision of unmixing is closely related with t...Hyperspectral unmixing is a powerful tool for the remote sensing image mining. Nonnegative matrix factorization (NMF) has been adopted to deal with this issue, while the precision of unmixing is closely related with the local minimizers of NMF. We present two novel initialization strategies that is based on CUR decomposition, which is physically meaningful. In the experimental test, NMF with the new initialization method is used to unmix the urban scene which was captured by airborne visible/infrared imaging spectrometer (AVIRIS) in 1997, numerical results show that the initialization methods work well.展开更多
AIM： To investigate the effect of 5, 7-dihydroxy-8- nitrochrysin （NOChR） on apoptosis of human gastric carcinoma SGC-7901 cell line.METHODS： SGC-7901 cells were cultured in vitro and the inhibitory effect of NOChR...AIM： To investigate the effect of 5, 7-dihydroxy-8- nitrochrysin （NOChR） on apoptosis of human gastric carcinoma SGC-7901 cell line.METHODS： SGC-7901 cells were cultured in vitro and the inhibitory effect of NOChR on proliferation of SGC-7901 cells was measured by using an Ml-r assay. NOChR-induced apoptosis rate of SGC-7901 cells was detected using flow cytometry （FCM） with PI staining. DNA ladder bands were observed by DNA agarose gel electrophoresis. The influence of NOChR on the proxisome proliferator-activated receptor-γ （PPARγ）, Bcl-2 and Bax protein expression of SGC-7901 cells was analyzed by Western blot.RESULTS： MIF assay showed that NOChR markedly inhibited proliferation of SGC-7901 cells in a dose- dependent manner, and when ICso was 4.14 μmol/L, the potency of NOChR was 10 times than that of lead compound, chrysin （ChR, IC50 was 40.56 μmol/L）, and was similar to 5-fluorouracil （5-FU, IC50 was 4.51 μmol/L）. FCM with propidium iodide （PI） staining demonstrated that the apoptosis rates of SGC-7901 cells treated with 1.25, 5.00 and 20.00 μmol/L NOChR for 48 h were 9.8% 4- 0.2%, 36.8% 4- 1.9% and 45.5% 4- 3.5%, respectively, and were significantly higher when treated with 5.00 and 20.00 μmol/L NOChR than that with 20.00 μmol/L ChR （12.9% 4- 1.5%）. DNA agarose gel electrophoresis showed that treatment of SGC-7901 cells with 20.00 μmol/L NOChR for 48 h resulted in typical DNA ladder bands of DNA of SGC-7901 cells, which could be eliminated by treating with 10.00 μmol/L GW9662, a blocker of PPARy. Western blot analysis revealed that after 24 h of treatment with 20.00 μmol/L NOChR, PPARgamma and Bax protein expression of SGC-7901 cells increased but Bcl-2 expression decreased; however, pre-incubation with 10.00 μmol/L GW9662 could efficiently antagonize and weaken the regulatory effect of 20.00 μmol/L NOChR on Bax and Bcl-2 protein expression of SGC-7901 cells. CONCLUSION： NOChR induces apoptosis of SGO7901 cell lines by activating PPARy and decreasing ratio of Bcl-2 to Bax.展开更多
According to geological conditions of No. 3 and No. 4 coal seams （namely A3 and B4） of the Pan＇er coal mine and the parameters of panels 11223, 11224, and 11124 with fully-mechanical coal mining, we built 2D simila...According to geological conditions of No. 3 and No. 4 coal seams （namely A3 and B4） of the Pan＇er coal mine and the parameters of panels 11223, 11224, and 11124 with fully-mechanical coal mining, we built 2D similar material simulation and FLAC3D numerical simulation models to investigate the development of mining-induced stress and the extraction effect of pressure-relief gas with large height and upward mining. Based on a comprehensive analysis of experimental data and observations, we obtained the deformation and breakage characteristics of strata overlying the coal seam, the development patterns of the mining-induced stress and fracture, and the size of the stress-relief area. The stress-relief effect was investigated and analyzed in consideration with mining height and three thick hard strata. Because of the group of three hard thick strata located in the main roof and the residual stress of mined panel 11124, the deformation, breakage, mining-induced stress and fracture development, and the stress-relief coefficient were discontinuous and asymmetrical. The breakage angle of the overlying strata, and the compressive and expansive zones of coal deformation were mainly controlled by the number, thickness, and strength of the hard stratum. Compared with the value of breakage angle derived by the traditional empirical method, the experimental value was lower than the traditional results by 3°-4°below the hard thick strata group, and by 13°-19° above the hard thick strata group. The amount of gas extracted from floor drainage roadway of B4 over 17 months was variable and the amount of gas per month differed considerably, being much smaller when panel 11223 influenced the area of the three hard thick strata. Generally, the stress-relief zone of No. 4 coal seam was small under the influence of the hard thick strata located in the main roof, which played an important role in delaying the breakage time and increasing the breakage space. In this study we gained understanding of the stress-relief mechanism influenced by the hard thick roof. The research results and engineering practice show that the main roof of the multiple hard thick strata is a critical factor in the design of panel layout and roadways for integrated coal exploitation and gas extraction, provides a theoretical basis for safe and high-efficient mining of coal resources.展开更多
5G network is expected to support massive user connections and exponentially increasing wireless services,which makes network security unprecedentedly important.Unlike traditional security-guaranteeing techniques whic...5G network is expected to support massive user connections and exponentially increasing wireless services,which makes network security unprecedentedly important.Unlike traditional security-guaranteeing techniques which rely heavily on cryptographic approaches at upper layers of the protocol stack,physical-layer security(PLS) solutions fully take advantages of the characteristics of wireless channels to degrade the received signal qualities at the malicious users,and realize keyless secure transmission via signal design and signal processing techniques.PLS avoids the difficulties in the distribution and management of secret keys,and provides flexible security levels through adaptive transmission protocol design.Moreover,PLS techniques match the features of 5G networks well.Therefore,the application of PLS to 5G networks is a promising solution to address the security threats.This article presents a comprehensive review of the state-of-the-art PLS techniques,and discusses their applications in 5G networks.We first summarize the principle and advantages of PLS techniques,and point out the reasons why PLS is suitable for 5G networks.Then,we review the existing PLS methods in literature,and highlight severalPLS solutions that are expected to be applied in 5G networks.Finally,we conclude this article and figure out some further research directions.展开更多
BACKGROUND Thyroid storm is resistant to conventional treatments including antithyroid drugs and 131I therapeutic means.Plasma exchange(PE)and double plasma molecular absorption system(DPMAS)can be used as an effectiv...BACKGROUND Thyroid storm is resistant to conventional treatments including antithyroid drugs and 131I therapeutic means.Plasma exchange(PE)and double plasma molecular absorption system(DPMAS)can be used as an effective treatment for thyroid storm with severe liver injury.CASE SUMMARY A 52-year-old woman presented with a 10-day history of nausea and vomiting accompanied by yellowing of the skin and mucosa.Further,her free T3(FT3)and FT4 levels were significantly elevated,whereas her thyrotropin level was reduced.After admission,her condition continued to deteriorate,and she presented with continued high fever,vomiting,palpitation,and shortness of breath.After being diagnosed with thyroid storm,the patient was immediately treated with PE combined with DPMAS.Her symptoms improved immediately.After three PE+DPMAS treatments,and she was discharged from the hospital.She was treated with methylprednisolone and methylthimidazole.After six months,the patient spontaneously discontinued methylthimidazole treatment.Her previous clinical manifestations and liver dysfunction reoccurred.The patient was treated with PE+DPMAS two times,and her condition rapidly improved.Liver histopathology indicated immunological liver injury.CONCLUSION Our experience suggests that PE combined with DPMAS can effectively relieve the development of thyroid storm.展开更多
Due to growing concerns regarding climate change and environmental protection,smart power generation has become essential for the economical and safe operation of both conventional thermal power plants and sustainable...Due to growing concerns regarding climate change and environmental protection,smart power generation has become essential for the economical and safe operation of both conventional thermal power plants and sustainable energy.Traditional first-principle model-based methods are becoming insufficient when faced with the ever-growing system scale and its various uncertainties.The burgeoning era of machine learning(ML)and data-driven control(DDC)techniques promises an improved alternative to these outdated methods.This paper reviews typical applications of ML and DDC at the level of monitoring,control,optimization,and fault detection of power generation systems,with a particular focus on uncovering how these methods can function in evaluating,counteracting,or withstanding the effects of the associated uncertainties.A holistic view is provided on the control techniques of smart power generation,from the regulation level to the planning level.The benefits of ML and DDC techniques are accordingly interpreted in terms of visibility,maneuverability,flexibility,profitability,and safety(abbreviated as the“5-TYs”),respectively.Finally,an outlook on future research and applications is presented.展开更多
AIM： To examine whether and how rosiglitazone enhances apoptosis induced by fluorouracil in human colon cancer （HT-29） cells. METHODS： Human colon cancer HT-29 cells were cultured in vitro and treated with fluorou...AIM： To examine whether and how rosiglitazone enhances apoptosis induced by fluorouracil in human colon cancer （HT-29） cells. METHODS： Human colon cancer HT-29 cells were cultured in vitro and treated with fluorouracil and/or rosiglitazone. Proliferation and growth of HT-29 cells were evaluated by MTF assay and trypan blue exclusion methods, respectively. The apoptosis of HT-29 cells was determined by acridine orange/ethidium bromide staining and flow cytometry using PI fluorescence staining. The expressions of peroxisome proliferator-activated receptor γ （PPARγ）, Bcl-2 and Bax in HT-29 cells were analyzed by Western blot. RESULTS： Although rosiglitazone at the concentration below 30 μmol/L for 72 h exerted almost no inhibitory effect on proliferation and growth of HT-29 cells, it could significantly enhance fluorouracil-induced HT-29 cell proliferation and growth inhibition. Furthermore, 10 μmol/L rosilitazone did not induce apoptosis of HT-29 cells but dramatically enhanced fluorouracil-induced apoptosis of HT-29 cells. However, rosiglitazone did not improve apoptosis induced by fluorouracil in HT-29 cells pretreated with GW9662, a PPARγ antagonist. Meanwhile, the expression of Bax and PPAR7 was upregulated, while the expression of Bcl-2 was down regulated in HT-29 cells treated with rosiglitazone in a time-dependent manner. However, the effect of rosiglitazone on Bcl-2 and Bax was blocked or diminished in the presence of GW9662. CONCLUSION： Rosiglitazone enhances fluorouracilinduced apoptosis of HT-29 cells by activating PPARγ.展开更多
Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury.Autologous mitochondrial transplantation is also beneficial in pediatric...Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury.Autologous mitochondrial transplantation is also beneficial in pediatric patients with cardiac ischemia-reperfusion injury.Thus,transplantation of functional exogenous mitochondria may be a promising therapeutic approach for ischemic disease.To explore the neuroprotective effect of mitochondria transplantation and determine the underlying mechanism in ischemic stroke,in this study we established a photo-thrombosis-induced mouse model of focal ischemia and administered freshly isolated mitochondria via the tail vein or to the injury site(in situ).Animal behavior tests,immunofluorescence staining,2,3,5-triphenyltetrazolium chloride(TTC)staining,mRNA-seq,and western blotting were used to assess mouse anxiety and memory,cortical infarct area,pyroptosis,and neurogenesis,respectively.Using bioinformatics analysis,western blotting,co-immunoprecipitation,and mass spectroscopy,we identified S100 calcium binding protein A9(S100A9)as a potential regulator of mitochondrial function and determined its possible interacting proteins.Interactions between exogenous and endogenous mitochondria,as well as the effect of exogenous mitochondria on recipient microglia,were assessed in vitro.Our data showed that:(1)mitochondrial transplantation markedly reduced mortality and improved emotional and cognitive function,as well as reducing infarct area,inhibiting pyroptosis,and promoting cortical neurogenesis;(2)microglial expression of S100A9 was markedly increased by ischemic injury and regulated mitochondrial function;(3)in vitro,exogenous mitochondria enhanced mitochondrial function,reduced redox stress,and regulated microglial polarization and pyroptosis by fusing with endogenous mitochondria;and(4)S100A9 promoted internalization of exogenous mitochondria by the microglia,thereby amplifying their pro-proliferation and anti-inflammatory effects.Taken together,our findings show that mitochondrial transplantation protects against the deleterious effects of ischemic stroke by suppressing pyroptosis and promoting neurogenesis,and that S100A9 plays a vital role in promoting internalization of exogenous mitochondria.展开更多
Zeolitic imidazolate frameworks(ZIFs)as smart drug delivery systems with microenvironment-triggered release have attracted much attention for tumor therapy.However,the exploration of ZIFs in biomedicine still encounte...Zeolitic imidazolate frameworks(ZIFs)as smart drug delivery systems with microenvironment-triggered release have attracted much attention for tumor therapy.However,the exploration of ZIFs in biomedicine still encounters many issues,such as inconvenient surface modification,fast drug release during blood circulation,undesired damage to major organs,and severe in vivo toxicity.To address the above issues,we developed an Mn-ZIF-90 nanosystem functionalized with an originally designed active-targeting and pH-responsive magnetic resonance imaging(MRI)Y1 receptor ligand[Asn28,Pro30,Trp32]-NPY(25-36)for imaging-guided tumor therapy.After Y1 receptor ligand modification,the Mn-ZIF-90 nanosystem exhibited high drug loading,better blood circulation stability,and dual breast cancer cell membrane and mitochondria targetability,further favoring specific microenvironment-triggered tumor therapy.Meanwhile,this nanosystem showed promising T1-weighted magnetic resonance imaging contrast in vivo in the tumor sites.Especially,this nanosystem with fast clean-up had almost no obvious toxicity and no damage occurred to the major organs in mice.Therefore,this nanosystem shows potential for use in imaging-guided tumor therapy.展开更多
AIM To examine the evidence behind the use of concentrated bone marrow aspirate(c BMA) in cartilage, bone, and tendon repair; establish proof of concept for the use of cB MA in these biologic environments; and provide...AIM To examine the evidence behind the use of concentrated bone marrow aspirate(c BMA) in cartilage, bone, and tendon repair; establish proof of concept for the use of cB MA in these biologic environments; and provide the level and quality of evidence substantiating the use of cB MA in the clinical setting.METHODS We conducted a systematic review according to PRISMA guidelines. EMBASE, MEDLINE, and Web of Knowledge databases were screened for the use of cB MA in the repair of cartilage, bone, and tendon repair. We extracted data on tissue type, cB MA preparation, cB MA concentration, study methods, outcomes, and level of evidence and reported the results in tables and text.RESULTS A total of 36 studies met inclusion/exclusion criteria and were included in this review. Thirty-one of 36(86%) studies reported the method of centrifugation and preparation of cB MA with 15(42%) studies reporting either a cell concentration or an increase from baseline. Variation of c BMA application was seen amongst the studies evaluated. Twenty-one of 36(58%) were level of evidence Ⅳ, 12/36(33%) were level of evidence Ⅲ, and 3/36(8%) were level of evidence Ⅱ. Studies evaluated full thickness chondral lesions(7 studies), osteochondral lesions(10 studies), osteoarthritis(5 studies), nonunion or fracture(9 studies), or tendon injuries(5 studies). Significant clinical improvement with the presence of hyaline-like values and lower incidence of fibrocartilage on T2 mapping was found in patients receiving cB MA in the treatment of cartilaginous lesions. Bone consolidation and time to bone union was improved in patients receiving cB MA. Enhanced healingrates, improved quality of the repair surface on ultrasound and magnetic resonance imaging, and a decreased risk of re-rupture was demonstrated in patients receiving cB MA as an adjunctive treatment in tendon repair. CONCLUSION The current literature demonstrates the potential benefits of utilizing c BMA for the repair of cartilaginous lesions, bony defects, and tendon injuries in the clinical setting. This study also demonstrates discrepancies between the literature with regards to various methods of centrifugation, variable cell count concentrations, and lack of standardized outcome measures. Future studies should attempt to examine the integral factors necessary for tissue regeneration and renewal including stem cells, growth factors and a biologic scaffold.展开更多
Synthesis of tetrahydrofuran and tetrahydropyran derivatives catalyzed by tungstophosphoric acid （H3PW12040） were conveniently performed with high yield from the corresponding unsaturated alcohols in ionic liquid. S...Synthesis of tetrahydrofuran and tetrahydropyran derivatives catalyzed by tungstophosphoric acid （H3PW12040） were conveniently performed with high yield from the corresponding unsaturated alcohols in ionic liquid. Sufuric acid （H2SO4）, trifluoromathanesulfonic acid （TfOH） and p-toluenesulfonic acid （TsOH） were also explored for preparing these products in ionic liquid. The catalysts and ionic liquid can be easily recovered and reused.展开更多
Transition metal sulfides(TMSs) are promising candidates for replacing graphite anode in LIBs. However,the low conductivity and structural collapse caused by the large volume change during lithium insertion and extrac...Transition metal sulfides(TMSs) are promising candidates for replacing graphite anode in LIBs. However,the low conductivity and structural collapse caused by the large volume change during lithium insertion and extraction greatly limit its application. Herein, we report a unique design of a two-dimensional(2 D) sandwich structure of N-doped carbon@Co9 S8@graphene(N–C@Co9 S8@G) with multilayer structure. Electrochemical tests reveal that the N–C@Co9 S8@G nanosheets possess a high reversible capacity(1009 mAhg^(-1) at 0.1 Ag^(-1)), and excellent rate capability(422mAhg^(-1) at 10 Ag^(-1)) and long cycle life(853 m Ahg^(-1) at 1Ag^(-1) for 500 cycles). Experimental studies reveal that capacitive storage contributes to the high reversible capacity. The lithium storage kinetics are studied by Galvanostatic intermittent titration technique(GITT) and electrochemical impedance spectroscopy(EIS). Meanwhile, the potential of N–C@Co9 S8@G anode in a full cell using Li Co O2 as the cathode is also demonstrated, exhibiting a high reversible capacity of 300mAhg^(-1) cycles at 0.1Ag^(-1). The strategy proposed in this work paves the way to engineering high performance anodes in LIBs.展开更多
基金supported by the National Natural Science Foundation of China,No.82071442 (to LS)a grant from the Jilin Provincial Department of Finance,No.JLSWSRCZX2021-004 (to LS)。
文摘Alzheimer's disease is an age-related neurodegenerative disorder with a complex and incompletely understood pathogenesis. Despite extensive research, a cure for Alzheimer's disease has not yet been found. Oxidative stress mediates excessive oxidative responses, and its involvement in Alzheimer's disease pathogenesis as a primary or secondary pathological event is widely accepted. As a member of the selenium-containing antioxidant enzyme family, glutathione peroxidase 4 reduces esterified phospholipid hydroperoxides to maintain cellular redox homeostasis. With the discovery of ferroptosis, the central role of glutathione peroxidase 4 in anti-lipid peroxidation in several diseases, including Alzheimer's disease, has received widespread attention. Increasing evidence suggests that glutathione peroxidase 4 expression is inhibited in the Alzheimer's disease brain, resulting in oxidative stress, inflammation, ferroptosis, and apoptosis, which are closely associated with pathological damage in Alzheimer's disease. Several therapeutic approaches, such as small molecule drugs, natural plant products, and non-pharmacological treatments, ameliorate pathological damage and cognitive function in Alzheimer's disease by promoting glutathione peroxidase 4 expression and enhancing glutathione peroxidase 4 activity. Therefore, glutathione peroxidase 4 upregulation may be a promising strategy for the treatment of Alzheimer's disease. This review provides an overview of the gene structure, biological functions, and regulatory mechanisms of glutathione peroxidase 4, a discussion on the important role of glutathione peroxidase 4 in pathological events closely related to Alzheimer's disease, and a summary of the advances in small-molecule drugs, natural plant products, and non-pharmacological therapies targeting glutathione peroxidase 4 for the treatment of Alzheimer's disease. Most prior studies on this subject used animal models, and relevant clinical studies are lacking. Future clinical trials are required to validate the therapeutic effects of strategies targeting glutathione peroxidase 4 in the treatment of Alzheimer's disease.
基金supported by the“CUG Scholar”Scientific Research Funds at China University of Geosciences(Wuhan)(No.2021230)supported by the National Natural Science Foundation of China(Nos.41922025,42204062)。
文摘INTRODUCTION.On January 7,2025,at 9:05 AM BJT,a MS6.8 earthquake(CENC epicenter:28.50°N,87.45°E)struck Dingri County,Xizang Province(hereinafter referred to as the Dingri mainshock).The inferred moment magnitude,based on regional/teleseismic waveform inversion and back-projection,is approximately MW7.1.Focal mechanism solutions,aftershock distribution,and field surveys indicate that the Dingri mainshock was a normal-faulting event,with a nearly north-south strike and a westward-dipping fault plane.
基金supported by the Natural Science Foundation of Shandong Province(ZR2021QH096,ZR2020MH024)the National Natural Science Foundation of China(82300354,882070345)+2 种基金the Academic Promotion Program of Shandong First Medical University(2019QL012)the Shandong Taishan Scholarship(to SHY)fund of Tianjin Zhongxin Pharmaceutical Group Co.,Ltd。
文摘Acute myocardial infarction(AMI)is a leading cause of death and disability worldwide and consists of two distinct cardiac pathologies:ST-elevation myocardial infarction(STEMI)and non-ST-elevation myocardial infarction(NSTEMI).[1]In China,AMI is widely recognized as a predominant cause of mortality in both urban and rural demographics,based on the 2022 Report on Cardiovascular Health and Diseases in China;moreover,its mortality rate has been reported to be rising,with a recurrence rate of 2.5%within one year.
基金supported by the Natural Science Foundation of Jiangsu Province,China(BK20242007)the Natural Science Foundation of China(32271916)the Jiangsu Agricultural Science and Technology Innovation Fund[CX(24)3048].
文摘bHLH transcription factors,widely exist in various plants,and are vital for the growth and development of these plants.Among them,many have been implicated in anthocyanin biosynthesis across various plants.In the present study,a PdbHLH57 gene,belonging to the bHLH IIIf group,was characterized,which was isolated and cloned from the colored-leaf poplar‘Zhongshancaiyun’(ZSCY).The cDNA sequence of PdbHLH57 was 1887 base pairs,and the protein encoded by PdbHLH57 had 628 amino acids,the isoelectric point and molecular weight of which were 6.26 and 69.75 kDa,respectively.Through bioinformatics analysis,PdbHLH57 has been classified into the IIIf bHLH subgroup,with many members of this subgroup known to participate in anthocyanin biosynthesis.The subcellular localization analysis conducted in the leaf protoplasts of‘ZSCY’revealed that the PdbHLH57 protein is specifically localized in the nucleus.The transcription activation analysis was also conducted,and the results showed that the PdbHLH57 protein had self-transcription activation.To better explore the functions of the PdbHLH57 protein,two parts of this protein(PdbHLH57-1,PdbHLH57-2)were split to detect their transcriptional activation activity.The results indicated that PdbHLH57-1(1-433aa)had self-transcription activation,and PdbHLH57-2(433-628aa)had no transcription activation.The expression of PdbHLH57 peaked in June during different developmental stages in‘ZSCY’,and it was most highly expressed in the phloem among various tissues.These findings offer a basis for understanding the role of PdbHLH57 in colored-leaf poplar.
基金supported by the National Natural Science Foundation of China(Nos.22173028,21873026).
文摘Due to insufficient energy density,supercapacitors(SCs)with preeminent-power and long cycle stability cannot be implemented in some practical applications.Exploring hybrid materials with redox activity to emerge high specific capacitance in ionic liquid(IL)electrolytes can solve this problem.Herein,we report a redox-organic molecule 2,6-diaminoanthraquinone(DAAQ)modified MXene(Ti3C2Tx)/Graphene(DAAQ-M/G)composite material.With the assist of graphene oxide(GO),MXene and graphene fabricate a three-dimensional(3D)interconnected structure as a conductive framework,which inhibits self-stacking of MXene monolayers and ensures high electronic conductivity.Meanwhile,DAAQ is loaded onto the M/G framework through covalent/non-covalent functionalization.The DAAQ as a spacer effectively enlarges the interlayer spacing of MXene nanosheets,and meanwhile produces reversible redox reactions during charge/discharge processes to provide additional Faradaic contribution to capacity.Therefore,the specific capacitance(capacity)of the DAAQ-M/G as the negative electrode material reaches to 226 F g^(-1)(306 C g^(-1))at 1 A g^(-1)in 1-ethyl-3-methylimidazolium tetrafluoroborate(EmimBF4)electrolyte.Furthermore,an asymmetric supercapacitor(ASC)is assembled using DAAQ-M/G as the negative electrode and self-prepared organic molecule hydroquinone modified reduced graphene oxide(HQ-RGO)material as the positive electrode,with a high energy density of 43 Wh kg^(-1)at high power density of 1669 Wkg^(-1).The ASC can maintain 80%of initial specific capacitance after 9000 cycles.This research can provide better support to develop advanced organic molecules-modified MXene composite materials for ionic liquid-based SCs.
基金supported by the National Key Research and Development Program of China(No.2022YFA1602200)the National Natural Science Foundation of China(Nos.12341501 and 12405174)the Hefei Comprehensive National Science Center for the strong support on the STCF key technology research project.
文摘Electron-positron colliders operating in the GeV center-of-mass range,or tau-charm energy region,have been proved to enable competitive frontier research due to several unique features.With the progress of high-energy physics in the last two decades,a new-generation Tau-Charm factory,called the Super Tau-Charm Facility(STCF),has been actively promoted by the particle physics community in China.STCF has the potential to address fundamental questions such as the essence of color confinement and the matter-antimatter asymmetry within the next decades.The main design goals of the STCF are a center-of-mass energy ranging from 2 to 7 GeV and a luminosity surpassing 5×10^(34)cm^(−2)s^(−1)that is optimized at a center-of-mass energy of 4 GeV,which is approximately 50 times that of the currently operating Tau-Charm factory-BEPCII.The STCF accelerator has two main parts:a double-ring collider with a crab-waist collision scheme and an injector that provides top-up injections for both electron and positron beams.As a typical third-generation electron-positron circular collider,the STCF accelerator faces many challenges in both accelerator physics and technology.In this paper,the conceptual design of the STCF accelerator complex is presented,including the ongoing efforts and plans for technological research and develop-ment,as well as the required infrastructure.The STCF project aims to secure support from the Chinese central government for its construction during the 15th Five-Year Plan(2026-2030).
基金supported by National Natural Science Foundation of China(52122811)。
文摘At 20:08 on September 18,2024,Beijing time,an earthquake of magnitude 4.7 occurred in Feidong,Anhui Province(31.98°N,117.6°E).The China Earthquake Early Warning Network presented the first early warning results 8.9 s after the earthquake.The China Earthquake Networks Center(CENC)released the automatic rapid report results 163 s after the earthquake and the official rapid report results 8 min after the earthquake.At the same time,the CENC reported a series of emergency products,including source parameters,seismic tectonics,historical earthquakes,focal mechanism,instrument seismic intensity and predicted intensity.The results showed that the earthquake was located at the junction of the southern section of the Tanlu Fault and the Feizhong Fault,with aftershocks distributed in the NEE direction.The focal mechanism solution indicates that the earthquake is essentially a strike-slip event.The predicted intensity in the vicinity of the epicenter reaches up to VI,involving 23towns that cover an area of about 1359 km^(2).Only one station near the epicenter shows a peak acceleration value greater than the fortification standard of the area,which may cause slight damage to some adjacent houses,consistent with the actual damage to buildings.
文摘From the perspective of village classification, a set of methods for accurately measuring the potential of rural settlement consolidation are proposed. Taking Feicheng in Shandong Province as the research area, combined with the corresponding planning, a classification and evaluation system for rural residential areas was constructed to classify rural residential areas from the four levels of natural resources, economy and society, supporting facilities and livability. The theoretical and practical potential of one type of rural settlements (relocating and merger village) is mainly calculated, and the potential scale, potential level and potential spatial distribution of different regions are analyzed. (Level and spatial distribution are analyzed. The results showed that the 621 rural settlements can be divided into five types: 148 urban-rural integration villages (URIV), 41 historical and cultural villages (HCV), 56 cluster developing villages (CDV), 155 keeping and limiting villages (KLV) and 221 relocating and merger villages (RMV). According to calculations, the theoretical potential of relocating and merger village areas was 1971.47 hm2, accounting for 19.52% of the total scale of Feicheng residential areas;the comprehensive correction coefficient of each region was 0.48 - 0.70, and the revised actual potential is 1082.68 hm2, accounting for the theoretical potential 54.92% of the total. There were large spatial differences in the scale of potential in different regions. In general, the theoretical and practical potential of the central and southern regions was higher than that of the northern regions.
文摘Hyperspectral unmixing is a powerful tool for the remote sensing image mining. Nonnegative matrix factorization (NMF) has been adopted to deal with this issue, while the precision of unmixing is closely related with the local minimizers of NMF. We present two novel initialization strategies that is based on CUR decomposition, which is physically meaningful. In the experimental test, NMF with the new initialization method is used to unmix the urban scene which was captured by airborne visible/infrared imaging spectrometer (AVIRIS) in 1997, numerical results show that the initialization methods work well.
文摘AIM： To investigate the effect of 5, 7-dihydroxy-8- nitrochrysin （NOChR） on apoptosis of human gastric carcinoma SGC-7901 cell line.METHODS： SGC-7901 cells were cultured in vitro and the inhibitory effect of NOChR on proliferation of SGC-7901 cells was measured by using an Ml-r assay. NOChR-induced apoptosis rate of SGC-7901 cells was detected using flow cytometry （FCM） with PI staining. DNA ladder bands were observed by DNA agarose gel electrophoresis. The influence of NOChR on the proxisome proliferator-activated receptor-γ （PPARγ）, Bcl-2 and Bax protein expression of SGC-7901 cells was analyzed by Western blot.RESULTS： MIF assay showed that NOChR markedly inhibited proliferation of SGC-7901 cells in a dose- dependent manner, and when ICso was 4.14 μmol/L, the potency of NOChR was 10 times than that of lead compound, chrysin （ChR, IC50 was 40.56 μmol/L）, and was similar to 5-fluorouracil （5-FU, IC50 was 4.51 μmol/L）. FCM with propidium iodide （PI） staining demonstrated that the apoptosis rates of SGC-7901 cells treated with 1.25, 5.00 and 20.00 μmol/L NOChR for 48 h were 9.8% 4- 0.2%, 36.8% 4- 1.9% and 45.5% 4- 3.5%, respectively, and were significantly higher when treated with 5.00 and 20.00 μmol/L NOChR than that with 20.00 μmol/L ChR （12.9% 4- 1.5%）. DNA agarose gel electrophoresis showed that treatment of SGC-7901 cells with 20.00 μmol/L NOChR for 48 h resulted in typical DNA ladder bands of DNA of SGC-7901 cells, which could be eliminated by treating with 10.00 μmol/L GW9662, a blocker of PPARy. Western blot analysis revealed that after 24 h of treatment with 20.00 μmol/L NOChR, PPARgamma and Bax protein expression of SGC-7901 cells increased but Bcl-2 expression decreased; however, pre-incubation with 10.00 μmol/L GW9662 could efficiently antagonize and weaken the regulatory effect of 20.00 μmol/L NOChR on Bax and Bcl-2 protein expression of SGC-7901 cells. CONCLUSION： NOChR induces apoptosis of SGO7901 cell lines by activating PPARy and decreasing ratio of Bcl-2 to Bax.
基金Acknowledgments This work is supported by the National Nature Science Foundation of China （51374011）.
文摘According to geological conditions of No. 3 and No. 4 coal seams （namely A3 and B4） of the Pan＇er coal mine and the parameters of panels 11223, 11224, and 11124 with fully-mechanical coal mining, we built 2D similar material simulation and FLAC3D numerical simulation models to investigate the development of mining-induced stress and the extraction effect of pressure-relief gas with large height and upward mining. Based on a comprehensive analysis of experimental data and observations, we obtained the deformation and breakage characteristics of strata overlying the coal seam, the development patterns of the mining-induced stress and fracture, and the size of the stress-relief area. The stress-relief effect was investigated and analyzed in consideration with mining height and three thick hard strata. Because of the group of three hard thick strata located in the main roof and the residual stress of mined panel 11124, the deformation, breakage, mining-induced stress and fracture development, and the stress-relief coefficient were discontinuous and asymmetrical. The breakage angle of the overlying strata, and the compressive and expansive zones of coal deformation were mainly controlled by the number, thickness, and strength of the hard stratum. Compared with the value of breakage angle derived by the traditional empirical method, the experimental value was lower than the traditional results by 3°-4°below the hard thick strata group, and by 13°-19° above the hard thick strata group. The amount of gas extracted from floor drainage roadway of B4 over 17 months was variable and the amount of gas per month differed considerably, being much smaller when panel 11223 influenced the area of the three hard thick strata. Generally, the stress-relief zone of No. 4 coal seam was small under the influence of the hard thick strata located in the main roof, which played an important role in delaying the breakage time and increasing the breakage space. In this study we gained understanding of the stress-relief mechanism influenced by the hard thick roof. The research results and engineering practice show that the main roof of the multiple hard thick strata is a critical factor in the design of panel layout and roadways for integrated coal exploitation and gas extraction, provides a theoretical basis for safe and high-efficient mining of coal resources.
基金supported in part by the National Natural Science Foundation of China under Grants No.61671369 and 61431011the National Science and Technology Major Project of China under Grant No.2016ZX03001012004+1 种基金the Open Research Fund of the State Key Laboratory of Integrated Services Networks,Xidian University,under Grant No.ISN18-02the Fundamental Research Funds for the Central Universities of China
文摘5G network is expected to support massive user connections and exponentially increasing wireless services,which makes network security unprecedentedly important.Unlike traditional security-guaranteeing techniques which rely heavily on cryptographic approaches at upper layers of the protocol stack,physical-layer security(PLS) solutions fully take advantages of the characteristics of wireless channels to degrade the received signal qualities at the malicious users,and realize keyless secure transmission via signal design and signal processing techniques.PLS avoids the difficulties in the distribution and management of secret keys,and provides flexible security levels through adaptive transmission protocol design.Moreover,PLS techniques match the features of 5G networks well.Therefore,the application of PLS to 5G networks is a promising solution to address the security threats.This article presents a comprehensive review of the state-of-the-art PLS techniques,and discusses their applications in 5G networks.We first summarize the principle and advantages of PLS techniques,and point out the reasons why PLS is suitable for 5G networks.Then,we review the existing PLS methods in literature,and highlight severalPLS solutions that are expected to be applied in 5G networks.Finally,we conclude this article and figure out some further research directions.
文摘BACKGROUND Thyroid storm is resistant to conventional treatments including antithyroid drugs and 131I therapeutic means.Plasma exchange(PE)and double plasma molecular absorption system(DPMAS)can be used as an effective treatment for thyroid storm with severe liver injury.CASE SUMMARY A 52-year-old woman presented with a 10-day history of nausea and vomiting accompanied by yellowing of the skin and mucosa.Further,her free T3(FT3)and FT4 levels were significantly elevated,whereas her thyrotropin level was reduced.After admission,her condition continued to deteriorate,and she presented with continued high fever,vomiting,palpitation,and shortness of breath.After being diagnosed with thyroid storm,the patient was immediately treated with PE combined with DPMAS.Her symptoms improved immediately.After three PE+DPMAS treatments,and she was discharged from the hospital.She was treated with methylprednisolone and methylthimidazole.After six months,the patient spontaneously discontinued methylthimidazole treatment.Her previous clinical manifestations and liver dysfunction reoccurred.The patient was treated with PE+DPMAS two times,and her condition rapidly improved.Liver histopathology indicated immunological liver injury.CONCLUSION Our experience suggests that PE combined with DPMAS can effectively relieve the development of thyroid storm.
文摘Due to growing concerns regarding climate change and environmental protection,smart power generation has become essential for the economical and safe operation of both conventional thermal power plants and sustainable energy.Traditional first-principle model-based methods are becoming insufficient when faced with the ever-growing system scale and its various uncertainties.The burgeoning era of machine learning(ML)and data-driven control(DDC)techniques promises an improved alternative to these outdated methods.This paper reviews typical applications of ML and DDC at the level of monitoring,control,optimization,and fault detection of power generation systems,with a particular focus on uncovering how these methods can function in evaluating,counteracting,or withstanding the effects of the associated uncertainties.A holistic view is provided on the control techniques of smart power generation,from the regulation level to the planning level.The benefits of ML and DDC techniques are accordingly interpreted in terms of visibility,maneuverability,flexibility,profitability,and safety(abbreviated as the“5-TYs”),respectively.Finally,an outlook on future research and applications is presented.
基金Supported by National Natural Science Foundation of China,No. 30472040
文摘AIM： To examine whether and how rosiglitazone enhances apoptosis induced by fluorouracil in human colon cancer （HT-29） cells. METHODS： Human colon cancer HT-29 cells were cultured in vitro and treated with fluorouracil and/or rosiglitazone. Proliferation and growth of HT-29 cells were evaluated by MTF assay and trypan blue exclusion methods, respectively. The apoptosis of HT-29 cells was determined by acridine orange/ethidium bromide staining and flow cytometry using PI fluorescence staining. The expressions of peroxisome proliferator-activated receptor γ （PPARγ）, Bcl-2 and Bax in HT-29 cells were analyzed by Western blot. RESULTS： Although rosiglitazone at the concentration below 30 μmol/L for 72 h exerted almost no inhibitory effect on proliferation and growth of HT-29 cells, it could significantly enhance fluorouracil-induced HT-29 cell proliferation and growth inhibition. Furthermore, 10 μmol/L rosilitazone did not induce apoptosis of HT-29 cells but dramatically enhanced fluorouracil-induced apoptosis of HT-29 cells. However, rosiglitazone did not improve apoptosis induced by fluorouracil in HT-29 cells pretreated with GW9662, a PPARγ antagonist. Meanwhile, the expression of Bax and PPAR7 was upregulated, while the expression of Bcl-2 was down regulated in HT-29 cells treated with rosiglitazone in a time-dependent manner. However, the effect of rosiglitazone on Bcl-2 and Bax was blocked or diminished in the presence of GW9662. CONCLUSION： Rosiglitazone enhances fluorouracilinduced apoptosis of HT-29 cells by activating PPARγ.
基金supported by the National Natural Science Foundation of China,Nos.82201621(to LS),31930048(to QY)and 81720108016(to QY),and 81971225(to CG)the Key Research and Development Project of Shaanxi Province,No.2022SF-189(to XS)the Tangdu Hospital Supporting Foundation,Nos.2021ZTXM-006(to LS)and 2021JSZH-006(to CG)。
文摘Transferring healthy and functional mitochondria to the lateral ventricles confers neuroprotection in a rat model of ischemia-reperfusion injury.Autologous mitochondrial transplantation is also beneficial in pediatric patients with cardiac ischemia-reperfusion injury.Thus,transplantation of functional exogenous mitochondria may be a promising therapeutic approach for ischemic disease.To explore the neuroprotective effect of mitochondria transplantation and determine the underlying mechanism in ischemic stroke,in this study we established a photo-thrombosis-induced mouse model of focal ischemia and administered freshly isolated mitochondria via the tail vein or to the injury site(in situ).Animal behavior tests,immunofluorescence staining,2,3,5-triphenyltetrazolium chloride(TTC)staining,mRNA-seq,and western blotting were used to assess mouse anxiety and memory,cortical infarct area,pyroptosis,and neurogenesis,respectively.Using bioinformatics analysis,western blotting,co-immunoprecipitation,and mass spectroscopy,we identified S100 calcium binding protein A9(S100A9)as a potential regulator of mitochondrial function and determined its possible interacting proteins.Interactions between exogenous and endogenous mitochondria,as well as the effect of exogenous mitochondria on recipient microglia,were assessed in vitro.Our data showed that:(1)mitochondrial transplantation markedly reduced mortality and improved emotional and cognitive function,as well as reducing infarct area,inhibiting pyroptosis,and promoting cortical neurogenesis;(2)microglial expression of S100A9 was markedly increased by ischemic injury and regulated mitochondrial function;(3)in vitro,exogenous mitochondria enhanced mitochondrial function,reduced redox stress,and regulated microglial polarization and pyroptosis by fusing with endogenous mitochondria;and(4)S100A9 promoted internalization of exogenous mitochondria by the microglia,thereby amplifying their pro-proliferation and anti-inflammatory effects.Taken together,our findings show that mitochondrial transplantation protects against the deleterious effects of ischemic stroke by suppressing pyroptosis and promoting neurogenesis,and that S100A9 plays a vital role in promoting internalization of exogenous mitochondria.
基金financially supported by Natural Science Foundation of China(No.81871411)National Key R&D Program of China(2018YFC0910601)+1 种基金Youth Innovation Promotion Association Foundation of CAS(2017340)The Science&Technology Bureau of Ningbo City(2015B11002).
文摘Zeolitic imidazolate frameworks(ZIFs)as smart drug delivery systems with microenvironment-triggered release have attracted much attention for tumor therapy.However,the exploration of ZIFs in biomedicine still encounters many issues,such as inconvenient surface modification,fast drug release during blood circulation,undesired damage to major organs,and severe in vivo toxicity.To address the above issues,we developed an Mn-ZIF-90 nanosystem functionalized with an originally designed active-targeting and pH-responsive magnetic resonance imaging(MRI)Y1 receptor ligand[Asn28,Pro30,Trp32]-NPY(25-36)for imaging-guided tumor therapy.After Y1 receptor ligand modification,the Mn-ZIF-90 nanosystem exhibited high drug loading,better blood circulation stability,and dual breast cancer cell membrane and mitochondria targetability,further favoring specific microenvironment-triggered tumor therapy.Meanwhile,this nanosystem showed promising T1-weighted magnetic resonance imaging contrast in vivo in the tumor sites.Especially,this nanosystem with fast clean-up had almost no obvious toxicity and no damage occurred to the major organs in mice.Therefore,this nanosystem shows potential for use in imaging-guided tumor therapy.
文摘AIM To examine the evidence behind the use of concentrated bone marrow aspirate(c BMA) in cartilage, bone, and tendon repair; establish proof of concept for the use of cB MA in these biologic environments; and provide the level and quality of evidence substantiating the use of cB MA in the clinical setting.METHODS We conducted a systematic review according to PRISMA guidelines. EMBASE, MEDLINE, and Web of Knowledge databases were screened for the use of cB MA in the repair of cartilage, bone, and tendon repair. We extracted data on tissue type, cB MA preparation, cB MA concentration, study methods, outcomes, and level of evidence and reported the results in tables and text.RESULTS A total of 36 studies met inclusion/exclusion criteria and were included in this review. Thirty-one of 36(86%) studies reported the method of centrifugation and preparation of cB MA with 15(42%) studies reporting either a cell concentration or an increase from baseline. Variation of c BMA application was seen amongst the studies evaluated. Twenty-one of 36(58%) were level of evidence Ⅳ, 12/36(33%) were level of evidence Ⅲ, and 3/36(8%) were level of evidence Ⅱ. Studies evaluated full thickness chondral lesions(7 studies), osteochondral lesions(10 studies), osteoarthritis(5 studies), nonunion or fracture(9 studies), or tendon injuries(5 studies). Significant clinical improvement with the presence of hyaline-like values and lower incidence of fibrocartilage on T2 mapping was found in patients receiving cB MA in the treatment of cartilaginous lesions. Bone consolidation and time to bone union was improved in patients receiving cB MA. Enhanced healingrates, improved quality of the repair surface on ultrasound and magnetic resonance imaging, and a decreased risk of re-rupture was demonstrated in patients receiving cB MA as an adjunctive treatment in tendon repair. CONCLUSION The current literature demonstrates the potential benefits of utilizing c BMA for the repair of cartilaginous lesions, bony defects, and tendon injuries in the clinical setting. This study also demonstrates discrepancies between the literature with regards to various methods of centrifugation, variable cell count concentrations, and lack of standardized outcome measures. Future studies should attempt to examine the integral factors necessary for tissue regeneration and renewal including stem cells, growth factors and a biologic scaffold.
文摘Synthesis of tetrahydrofuran and tetrahydropyran derivatives catalyzed by tungstophosphoric acid （H3PW12040） were conveniently performed with high yield from the corresponding unsaturated alcohols in ionic liquid. Sufuric acid （H2SO4）, trifluoromathanesulfonic acid （TfOH） and p-toluenesulfonic acid （TsOH） were also explored for preparing these products in ionic liquid. The catalysts and ionic liquid can be easily recovered and reused.
基金financially supported by the National Natural Science Foundation of China (No. 51972182 and 61971252)the Shandong Provincial Science Foundation (ZR2019BF008 and ZR2017JL021)+1 种基金the Youth Innovation Team Project of Shandong Provincial Education Department (2020KJN015)the “Distinguished Taishan Scholar” project。
文摘Transition metal sulfides(TMSs) are promising candidates for replacing graphite anode in LIBs. However,the low conductivity and structural collapse caused by the large volume change during lithium insertion and extraction greatly limit its application. Herein, we report a unique design of a two-dimensional(2 D) sandwich structure of N-doped carbon@Co9 S8@graphene(N–C@Co9 S8@G) with multilayer structure. Electrochemical tests reveal that the N–C@Co9 S8@G nanosheets possess a high reversible capacity(1009 mAhg^(-1) at 0.1 Ag^(-1)), and excellent rate capability(422mAhg^(-1) at 10 Ag^(-1)) and long cycle life(853 m Ahg^(-1) at 1Ag^(-1) for 500 cycles). Experimental studies reveal that capacitive storage contributes to the high reversible capacity. The lithium storage kinetics are studied by Galvanostatic intermittent titration technique(GITT) and electrochemical impedance spectroscopy(EIS). Meanwhile, the potential of N–C@Co9 S8@G anode in a full cell using Li Co O2 as the cathode is also demonstrated, exhibiting a high reversible capacity of 300mAhg^(-1) cycles at 0.1Ag^(-1). The strategy proposed in this work paves the way to engineering high performance anodes in LIBs.
