Objective To investigate whether 2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside(TSG)ameliorated polycystic ovary syndrome(PCOS)-like characteristics by inhibiting inflammation.Methods PCOS models were established ...Objective To investigate whether 2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside(TSG)ameliorated polycystic ovary syndrome(PCOS)-like characteristics by inhibiting inflammation.Methods PCOS models were established by injecting subcutaneously with dehydroepiandrosterone into female Sprague-Dawley rats,followed by receiving intraperitoneal injection of TSG.The granular cells(GCs)KGN were transfected with small interfering RNAs(si-NC and si-CYP19A1).The cells were preincubated with lipopolysaccharide(LPS)and then treated with or without TSG.The estrous cycle was monitored using vaginal exfoliated cells.The morphology of ovarian follicles was analyzed by H&E staining.ELISA was used to analyze estradiol(E2),testosterone(T),follicle stimulating hormone(FSH),luteinizing hormone(LH),IL-6,TNF-α,AGEs,CRP and Omentin-1 levels in serum.Immunohistochemistry was performed to analyze PCNA and CYP19A1 expressions in the GCs of ovaries.Tunel staining was executed to detect the apoptosis of GCs.Quantitative polymerase chain reaction(qPCR)and Western blot were implemented to measure the expression of CYP19A1 in the ovaries and transfected cells.qPCR was used to analyze the expression of IL-6 and TNF-αin the transfected cells treated with LPS and TSG.Results The estrous cycles were restored in TSG group.Compared with model group,the sinus follicles were reduced and corpus luteums were increased in TSG group.TSG group showed increased E2,and decreased T and LH,compared with model group.Pro-inflammatory factors(IL-6,TNF-α,CRP and AGEs)were decreased,and anti-inflammatory factor(Omentin-1)was increased in TSG group compared with those in model group.TSG could partially inhibit decrease of PNCA-positive GCs and increase of Tunel-positive GCs caused by PCOS.The CYP19A1 expression of GCs in TSG group was upregulated compared with model group.The expressions of IL-6 and TNFαin si-CYP19A1 cells were increased compared with si-NC cells.Compared with cells(si-NC and si-CYP19A1)treated without LPS,the expressions of IL-6 and TNF-αcells were increased,and the expression of CYP19A1 was downregulated in LPS-preincubated cells.Compared with cells treated with LPS,the expression of IL-6 and TNF-αwere decreased,and the expression of CYP19A1 was increased in cells treated with LPS and TSG.Compared with si-NC cells treated with LPS and TSG,the expressions of IL-6 and TNF-αcells were increased in the si-CYP19A1 cells treated with LPS and TSG.Conclusion TSG could alleviate PCOS-like characteristics by increasing the expression of CYP19A1 in GCs to inhibit inflammatory response.展开更多
In order to investigate the behavior characteristics of asphalt components during the process of nano-cracks temperature self-healing(NTS),molecular simulation technology was used to simulate the temperature self-heal...In order to investigate the behavior characteristics of asphalt components during the process of nano-cracks temperature self-healing(NTS),molecular simulation technology was used to simulate the temperature self-healing of asphalt.Based on the determination of asphalts(virgin asphalt and aged asphalt),the proportional changes of asphalt components were confirmed.The distribution characteristics of asphalt components were obtained by marking different components in the process of NTS.At the same time,the rationality of the micro simulation findings was confirmed by asphalt performance tests and infrared spectroscopy results.The results show that different asphalt components have different behavior characteristics in the process of NTS.The content of asphaltene and resin plays a key role in the process of NTS,while saturation and aromatics play an active role in the temperature self-healing before and after aging.The NTS is the result of the comprehensive action of different components,and the self-healing efficiency is proportional to the relative molecular mass.The effect of aging on the NTS is also realized by changing the proportion of asphalt components.展开更多
Based on drilling,mud logging,core,seismic and imaging logging data,this paper studies the identification and evolution process of negative inversion structures in the Carboniferous buried hills in the No.1 and No.2 f...Based on drilling,mud logging,core,seismic and imaging logging data,this paper studies the identification and evolution process of negative inversion structures in the Carboniferous buried hills in the No.1 and No.2 fault zones of Weixinan Sag,Beibu Gulf Basin,China,and reveals the controls of these structures on high-quality reservoirs.The No.2 fault zone develops significant negative inversion structures in the Carboniferous buried hills,as a result of multi-stage transformations of compressive-tensile stress fields in the period from the Late Hercynian to the Himalayan.The Hercynian carbonates laid the material basis for the formation of high-quality reservoirs.The negative inversion structures mainly control the development of high-quality reservoirs in buried hills through:(1)creating large-scale fractures to increase reservoir space and improve oil-gas flow pathways;(2)regulating stratigraphic differential denudation to highlight dominant lithology for later reservoir transformation;(3)shaping the paleogeomorphological highlands to provide favorable conditions for superficial karstification.The negative inversion structures form a high-quality,composite reservoir space with the synergistic existence of superficial dissolution fractures/cavities and burial-enhanced karst systems through the coupling of fracture network creation,formation denudation screening and multi-stage karst transformation.The research results have guided the breakthrough of the first exploratory well with a daily oil production over 1000 m^(3)in carbonate buried-hill reservoir in the Beibu Gulf Basin,and provide referential geological basis for finding more reserves and achieving higher production in the Carboniferous buried hills in the Weixinan Sag.展开更多
The perceived resilience of rural households has attracted considerable attention among both practitioners and researchers.However,a research gap still exists regarding its quantitative evaluation,place-based characte...The perceived resilience of rural households has attracted considerable attention among both practitioners and researchers.However,a research gap still exists regarding its quantitative evaluation,place-based characteristics,and impact mechanisms in areas threatened by multiple hazards.Based on data from 399 household questionnaire in typical villages of Wenchuan County,the epicenter of the Wenchuan earthquake on May 12,2008,a conceptual indicator framework of perceived resilience was established.Factor analysis was used to quantitatively evaluate the perceived resilience of rural households at different altitude gradient areas.A multiple linear regression model was used to describe the determinants of perceived resilience among different groups of households with disparate disaster experiences.The results show that:(1)A four-dimensional framework encompassing pre-disaster preventive capability,disaster coping capacity,post-disaster adaptive capacity,and learning and transition capability was identified by factor analysis for the quantitative measurement of the perceived resilience of rural households.(2)Their perceived resilience decreases with increasing altitude;the average perceived resilience score of households in the valley area is 1.5 times higher than that in the high mountains area.(3)Rural households with more experience of natural disasters exhibited higher levels of perceived resilience;however,this trend was reversed in the high mountains area.(4)The perceived resilience of households was significantly affected by most of the examined disaster preparedness indicators and a few of the examined personal characteristics indicators.For improving perceived resilience of rural households living in the study area,it is recommended to establish psychological crisis intervention networks in the valley area,promote more diversified livelihood strategies in the mid-mountains area,and mitigate the impacts of meteorological disasters in the high mountains area,respectively.展开更多
目的探讨Apelin/APJ(putative receptor protein related to the angiotensin receptor AT1)系统与小儿神经母细胞瘤(neuroblastoma,NB)发生发展的关系。方法收集10例NB患儿术后肿瘤组织及对应的癌旁肾上腺组织,采用免疫组化法检测Apeli...目的探讨Apelin/APJ(putative receptor protein related to the angiotensin receptor AT1)系统与小儿神经母细胞瘤(neuroblastoma,NB)发生发展的关系。方法收集10例NB患儿术后肿瘤组织及对应的癌旁肾上腺组织,采用免疫组化法检测Apelin及APJ蛋白在肿瘤及癌旁组织中的表达;Real-time PCR方法和Western blotting技术检测Apelin/APJ mRNA及蛋白的表达水平,采用ELISA方法检测NB患儿及健康儿童血浆中Apelin-36的水平。结果在收集的NB病例中,免疫组化结果显示,Apelin蛋白主要定位于神经母细胞瘤的细胞浆,呈强阳性表达,而APJ蛋白则主要在肿瘤胞核内表达,呈棕黄色颗粒,表达强阳性或中等阳性,两者均在对照组癌旁肾上腺组织中表达呈阴性或者弱阳性;与瘤旁组织比较,NB组织中Apelin、APJ mRNA和Apelin及APJ蛋白的表达水平均显著升高,且差异有统计学意义(P<0.05)。NB患儿血浆Apelin-36的水平显著高于健康儿童对照组的水平(P<0.05)。结论Apelin/APJ系统在小儿神经母细胞瘤组织中高度表达,提示该系统与NB的发生发展密切相关。展开更多
文摘Objective To investigate whether 2,3,5,4'-tetrahydroxystilbene-2-O-β-glucoside(TSG)ameliorated polycystic ovary syndrome(PCOS)-like characteristics by inhibiting inflammation.Methods PCOS models were established by injecting subcutaneously with dehydroepiandrosterone into female Sprague-Dawley rats,followed by receiving intraperitoneal injection of TSG.The granular cells(GCs)KGN were transfected with small interfering RNAs(si-NC and si-CYP19A1).The cells were preincubated with lipopolysaccharide(LPS)and then treated with or without TSG.The estrous cycle was monitored using vaginal exfoliated cells.The morphology of ovarian follicles was analyzed by H&E staining.ELISA was used to analyze estradiol(E2),testosterone(T),follicle stimulating hormone(FSH),luteinizing hormone(LH),IL-6,TNF-α,AGEs,CRP and Omentin-1 levels in serum.Immunohistochemistry was performed to analyze PCNA and CYP19A1 expressions in the GCs of ovaries.Tunel staining was executed to detect the apoptosis of GCs.Quantitative polymerase chain reaction(qPCR)and Western blot were implemented to measure the expression of CYP19A1 in the ovaries and transfected cells.qPCR was used to analyze the expression of IL-6 and TNF-αin the transfected cells treated with LPS and TSG.Results The estrous cycles were restored in TSG group.Compared with model group,the sinus follicles were reduced and corpus luteums were increased in TSG group.TSG group showed increased E2,and decreased T and LH,compared with model group.Pro-inflammatory factors(IL-6,TNF-α,CRP and AGEs)were decreased,and anti-inflammatory factor(Omentin-1)was increased in TSG group compared with those in model group.TSG could partially inhibit decrease of PNCA-positive GCs and increase of Tunel-positive GCs caused by PCOS.The CYP19A1 expression of GCs in TSG group was upregulated compared with model group.The expressions of IL-6 and TNFαin si-CYP19A1 cells were increased compared with si-NC cells.Compared with cells(si-NC and si-CYP19A1)treated without LPS,the expressions of IL-6 and TNF-αcells were increased,and the expression of CYP19A1 was downregulated in LPS-preincubated cells.Compared with cells treated with LPS,the expression of IL-6 and TNF-αwere decreased,and the expression of CYP19A1 was increased in cells treated with LPS and TSG.Compared with si-NC cells treated with LPS and TSG,the expressions of IL-6 and TNF-αcells were increased in the si-CYP19A1 cells treated with LPS and TSG.Conclusion TSG could alleviate PCOS-like characteristics by increasing the expression of CYP19A1 in GCs to inhibit inflammatory response.
基金Funded by the Fundamental Research Funds for the Central Universities(No.2572021AW10)。
文摘In order to investigate the behavior characteristics of asphalt components during the process of nano-cracks temperature self-healing(NTS),molecular simulation technology was used to simulate the temperature self-healing of asphalt.Based on the determination of asphalts(virgin asphalt and aged asphalt),the proportional changes of asphalt components were confirmed.The distribution characteristics of asphalt components were obtained by marking different components in the process of NTS.At the same time,the rationality of the micro simulation findings was confirmed by asphalt performance tests and infrared spectroscopy results.The results show that different asphalt components have different behavior characteristics in the process of NTS.The content of asphaltene and resin plays a key role in the process of NTS,while saturation and aromatics play an active role in the temperature self-healing before and after aging.The NTS is the result of the comprehensive action of different components,and the self-healing efficiency is proportional to the relative molecular mass.The effect of aging on the NTS is also realized by changing the proportion of asphalt components.
基金Supported by the Hainan Provincial Science and Technology Special Project(ZDYF2025GXJS013)CNOOC Zhanjiang Branch Project(CCL2023ZJFN0540).
文摘Based on drilling,mud logging,core,seismic and imaging logging data,this paper studies the identification and evolution process of negative inversion structures in the Carboniferous buried hills in the No.1 and No.2 fault zones of Weixinan Sag,Beibu Gulf Basin,China,and reveals the controls of these structures on high-quality reservoirs.The No.2 fault zone develops significant negative inversion structures in the Carboniferous buried hills,as a result of multi-stage transformations of compressive-tensile stress fields in the period from the Late Hercynian to the Himalayan.The Hercynian carbonates laid the material basis for the formation of high-quality reservoirs.The negative inversion structures mainly control the development of high-quality reservoirs in buried hills through:(1)creating large-scale fractures to increase reservoir space and improve oil-gas flow pathways;(2)regulating stratigraphic differential denudation to highlight dominant lithology for later reservoir transformation;(3)shaping the paleogeomorphological highlands to provide favorable conditions for superficial karstification.The negative inversion structures form a high-quality,composite reservoir space with the synergistic existence of superficial dissolution fractures/cavities and burial-enhanced karst systems through the coupling of fracture network creation,formation denudation screening and multi-stage karst transformation.The research results have guided the breakthrough of the first exploratory well with a daily oil production over 1000 m^(3)in carbonate buried-hill reservoir in the Beibu Gulf Basin,and provide referential geological basis for finding more reserves and achieving higher production in the Carboniferous buried hills in the Weixinan Sag.
基金funded by the National Natural Science Foundation of China(grant number:42001244)Chengdu Science and Technology Program(grant number:2024-YF05-01061-SN)Research Program of Institute of Mountain Hazards and Environment,Chinese Academy of Sciences(grant number:E4K2060060)。
文摘The perceived resilience of rural households has attracted considerable attention among both practitioners and researchers.However,a research gap still exists regarding its quantitative evaluation,place-based characteristics,and impact mechanisms in areas threatened by multiple hazards.Based on data from 399 household questionnaire in typical villages of Wenchuan County,the epicenter of the Wenchuan earthquake on May 12,2008,a conceptual indicator framework of perceived resilience was established.Factor analysis was used to quantitatively evaluate the perceived resilience of rural households at different altitude gradient areas.A multiple linear regression model was used to describe the determinants of perceived resilience among different groups of households with disparate disaster experiences.The results show that:(1)A four-dimensional framework encompassing pre-disaster preventive capability,disaster coping capacity,post-disaster adaptive capacity,and learning and transition capability was identified by factor analysis for the quantitative measurement of the perceived resilience of rural households.(2)Their perceived resilience decreases with increasing altitude;the average perceived resilience score of households in the valley area is 1.5 times higher than that in the high mountains area.(3)Rural households with more experience of natural disasters exhibited higher levels of perceived resilience;however,this trend was reversed in the high mountains area.(4)The perceived resilience of households was significantly affected by most of the examined disaster preparedness indicators and a few of the examined personal characteristics indicators.For improving perceived resilience of rural households living in the study area,it is recommended to establish psychological crisis intervention networks in the valley area,promote more diversified livelihood strategies in the mid-mountains area,and mitigate the impacts of meteorological disasters in the high mountains area,respectively.
文摘目的探讨Apelin/APJ(putative receptor protein related to the angiotensin receptor AT1)系统与小儿神经母细胞瘤(neuroblastoma,NB)发生发展的关系。方法收集10例NB患儿术后肿瘤组织及对应的癌旁肾上腺组织,采用免疫组化法检测Apelin及APJ蛋白在肿瘤及癌旁组织中的表达;Real-time PCR方法和Western blotting技术检测Apelin/APJ mRNA及蛋白的表达水平,采用ELISA方法检测NB患儿及健康儿童血浆中Apelin-36的水平。结果在收集的NB病例中,免疫组化结果显示,Apelin蛋白主要定位于神经母细胞瘤的细胞浆,呈强阳性表达,而APJ蛋白则主要在肿瘤胞核内表达,呈棕黄色颗粒,表达强阳性或中等阳性,两者均在对照组癌旁肾上腺组织中表达呈阴性或者弱阳性;与瘤旁组织比较,NB组织中Apelin、APJ mRNA和Apelin及APJ蛋白的表达水平均显著升高,且差异有统计学意义(P<0.05)。NB患儿血浆Apelin-36的水平显著高于健康儿童对照组的水平(P<0.05)。结论Apelin/APJ系统在小儿神经母细胞瘤组织中高度表达,提示该系统与NB的发生发展密切相关。
