Nitrogen deposition and water tables are important factors to control soil microbial community structure.However,the specific effects and mechanisms of nitrogen deposition and water tables coupling on bacterial divers...Nitrogen deposition and water tables are important factors to control soil microbial community structure.However,the specific effects and mechanisms of nitrogen deposition and water tables coupling on bacterial diversity,abundance,and community structure in arid alpine wetlands remain unclear.The nitrogen deposition(0,10,and 20 kg N/(hm^(2)•a))experiments were conducted in the Bayinbulak alpine wetland with different water tables(perennial flooding,seasonal waterlogging,and perennial drying).The 16S rRNA(ribosomal ribonucleic acid)gene sequencing technology was employed to analyze the changes in bacterial community diversity,network structure,and function in the soil.Results indicated that bacterial diversity was the highest under seasonal waterlogging condition.However,nitrogen deposition only affected the bacterial Chao1 and beta diversity indices under seasonal waterlogging condition.The abundance of bacterial communities under different water tables showed significant differences at the phylum and genus levels.The dominant phylum,Proteobacteria,was sensitive to soil moisture and its abundance decreased with decreasing water tables.Although nitrogen deposition led to changes in bacterial abundance,such changes were small compared with the effects of water tables.Nitrogen deposition with 10 kg N/(hm^(2)•a)decreased bacterial edge number,average path length,and robustness.However,perennial flooding and drying conditions could simply resist environmental changes caused by 20 kg N/(hm^(2)•a)nitrogen deposition and their network structure remain unchanged.The sulfur cycle function was dominant under perennial flooding condition,and carbon and nitrogen cycle functions were dominant under seasonal waterlogging and perennial drying conditions.Nitrogen application increased the potential function of part of nitrogen cycle and decreased the potential function of sulfur cycle in bacterial community.In summary,composition of bacterial community in the arid alpine wetland was determined by water tables,and diversity of bacterial community was inhibited by a lower water table.Effect of nitrogen deposition on bacterial community structure and function depended on water tables.展开更多
To the electronics wastewater with high salinity,the fouling propensity of effluent from the membrane bioreactor（ MBR） system played an important role for the design and long-term operational sustainability of the M...To the electronics wastewater with high salinity,the fouling propensity of effluent from the membrane bioreactor（ MBR） system played an important role for the design and long-term operational sustainability of the MBR ＋ Reverse Osmosis（ RO） system. In this text,the effluent quality,settling performance and membrane fouling were researched with three dimension excitation-emission fluorescence spectra（ EEM） and scanning electron microscope（ SEM）-Energy Dispersive Spectrometer（ EDS）. The results showed that there were few organics with a strong adhesion in the effluent. The metal ions in the effluent not only had a complexation reaction with the macromolecules fulvic acid and protein but also with silicon colloid. It caused a compact fouling layer on the RO membrane surface. But the organics and most of inorganic matters could form settling of flocs and be removed from the effluent by improving p H of the effluent,which would relieve the membrane fouling. In addition,the removal of metal ions in the fouling layer was critical for enhancing the cleaning efficiency of RO membrane.展开更多
目的:探讨Nemo样激酶(Nemo-like kinase,NLK)以及磷酸酶和张力蛋白同源物(phosphatase and tensin homolog deleted on chromosome ten,PTEN)在头颈部鳞癌(head and neck squamous cell carcinoma,HNSCC)中的表达及与临床病理特征的关系...目的:探讨Nemo样激酶(Nemo-like kinase,NLK)以及磷酸酶和张力蛋白同源物(phosphatase and tensin homolog deleted on chromosome ten,PTEN)在头颈部鳞癌(head and neck squamous cell carcinoma,HNSCC)中的表达及与临床病理特征的关系,并初步探讨NLK与PTEN之间的相关性。方法:从癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库提取HNSCC患者相关基因表达和临床特征数据,使用R语言和Bioconductor包进行数据处理,应用在线平台进行数据分析。选取32例HNSCC患者标本,应用免疫组织化学方法检测NLK和PTEN的表达,并验证两者间的相关性。结果:通过对TCGA数据库收集的520例病例的分析发现,NLK和PTEN在HNSCC中有显著的表达改变,并且在性别、临床分期、T分期、N分期及HPV感染队列中存在显著差异。在不同部位的HNSCC中,NLK和PTEN也存在表达差异。免疫组织化学染色结果与TCGA数据分析均显示,NLK和PTEN的表达呈负相关。结论:NLK和PTEN在HNSCC不同临床病理特征队列中存在表达差异,且两者表达呈负相关。展开更多
基金supported by the National Natural Science Foundation of China(31960258)the Graduate Research Innovation Project of Xinjiang Uygur Autonomous Region(XJ2023G119).
文摘Nitrogen deposition and water tables are important factors to control soil microbial community structure.However,the specific effects and mechanisms of nitrogen deposition and water tables coupling on bacterial diversity,abundance,and community structure in arid alpine wetlands remain unclear.The nitrogen deposition(0,10,and 20 kg N/(hm^(2)•a))experiments were conducted in the Bayinbulak alpine wetland with different water tables(perennial flooding,seasonal waterlogging,and perennial drying).The 16S rRNA(ribosomal ribonucleic acid)gene sequencing technology was employed to analyze the changes in bacterial community diversity,network structure,and function in the soil.Results indicated that bacterial diversity was the highest under seasonal waterlogging condition.However,nitrogen deposition only affected the bacterial Chao1 and beta diversity indices under seasonal waterlogging condition.The abundance of bacterial communities under different water tables showed significant differences at the phylum and genus levels.The dominant phylum,Proteobacteria,was sensitive to soil moisture and its abundance decreased with decreasing water tables.Although nitrogen deposition led to changes in bacterial abundance,such changes were small compared with the effects of water tables.Nitrogen deposition with 10 kg N/(hm^(2)•a)decreased bacterial edge number,average path length,and robustness.However,perennial flooding and drying conditions could simply resist environmental changes caused by 20 kg N/(hm^(2)•a)nitrogen deposition and their network structure remain unchanged.The sulfur cycle function was dominant under perennial flooding condition,and carbon and nitrogen cycle functions were dominant under seasonal waterlogging and perennial drying conditions.Nitrogen application increased the potential function of part of nitrogen cycle and decreased the potential function of sulfur cycle in bacterial community.In summary,composition of bacterial community in the arid alpine wetland was determined by water tables,and diversity of bacterial community was inhibited by a lower water table.Effect of nitrogen deposition on bacterial community structure and function depended on water tables.
基金Supported by the Collaborative-innovative Project of Beijing Chaoyang District(XC1619)
文摘To the electronics wastewater with high salinity,the fouling propensity of effluent from the membrane bioreactor（ MBR） system played an important role for the design and long-term operational sustainability of the MBR ＋ Reverse Osmosis（ RO） system. In this text,the effluent quality,settling performance and membrane fouling were researched with three dimension excitation-emission fluorescence spectra（ EEM） and scanning electron microscope（ SEM）-Energy Dispersive Spectrometer（ EDS）. The results showed that there were few organics with a strong adhesion in the effluent. The metal ions in the effluent not only had a complexation reaction with the macromolecules fulvic acid and protein but also with silicon colloid. It caused a compact fouling layer on the RO membrane surface. But the organics and most of inorganic matters could form settling of flocs and be removed from the effluent by improving p H of the effluent,which would relieve the membrane fouling. In addition,the removal of metal ions in the fouling layer was critical for enhancing the cleaning efficiency of RO membrane.
文摘目的:探讨Nemo样激酶(Nemo-like kinase,NLK)以及磷酸酶和张力蛋白同源物(phosphatase and tensin homolog deleted on chromosome ten,PTEN)在头颈部鳞癌(head and neck squamous cell carcinoma,HNSCC)中的表达及与临床病理特征的关系,并初步探讨NLK与PTEN之间的相关性。方法:从癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库提取HNSCC患者相关基因表达和临床特征数据,使用R语言和Bioconductor包进行数据处理,应用在线平台进行数据分析。选取32例HNSCC患者标本,应用免疫组织化学方法检测NLK和PTEN的表达,并验证两者间的相关性。结果:通过对TCGA数据库收集的520例病例的分析发现,NLK和PTEN在HNSCC中有显著的表达改变,并且在性别、临床分期、T分期、N分期及HPV感染队列中存在显著差异。在不同部位的HNSCC中,NLK和PTEN也存在表达差异。免疫组织化学染色结果与TCGA数据分析均显示,NLK和PTEN的表达呈负相关。结论:NLK和PTEN在HNSCC不同临床病理特征队列中存在表达差异,且两者表达呈负相关。
