OBJECTIVE:To investigate the therapeutic effects of Huai'er(Trametes)on psoriasis by identifying specific molecular targets and pathways involved in regulating keratinocyte behavior and immune responses.METHODS:Ce...OBJECTIVE:To investigate the therapeutic effects of Huai'er(Trametes)on psoriasis by identifying specific molecular targets and pathways involved in regulating keratinocyte behavior and immune responses.METHODS:Cellular experiments were conducted using human keratinocyte cell line(Ha Ca T)keratinocytes to evaluate the effects of Huai'er(Trametes)on cell proliferation,migration,and inflammatory factor expression.Additionally,an imiquimod(IMQ)-induced psoriasis-like mouse model was established to assess the therapeutic efficacy of Huai'er(Trametes)in vivo.Network pharmacology and transcriptomic analyses were performed to identify potential targets and pathways.RESULTS:Huai'er(Trametes)significantly inhibited Ha Ca T keratinocyte proliferation and migration in vitro,as evidenced by reduced 5-ethynyl-2′-deoxyuridine incorporation and wound healing rates.In the IMQ-induced psoriasis-like mouse model,Huai'er(Trametes)treatment reduced erythema,scaling,and dermal infiltration of inflammatory cells,demonstrating its efficacy in alleviating psoriasis symptoms.Network pharmacology and transcriptomic analyses identified signal transducer and activator of transcription 1(STAT1)as a central target modulated by Huai'er(Trametes).This regulation was associated with decreased expression of proinflammatory cytokines,including interleukin-17A and tumor necrosis factor alpha,both in vitro and in vivo.CONCLUSION:This study demonstrates that Huai'er(Trametes)exerts therapeutic effects on psoriasis by modulating STAT1,thereby inhibiting keratinocyte proliferation and inflammatory responses.The findings provide a foundation for further research into the potential of Huai'er(Trametes)as a treatment for psoriasis.展开更多
基金the Scientific Research Project of Hunan Provincial Health Commission:Effects and Mechanisms of Huaiqi Huang on the Proliferation and Migration of Psoriasis HaCaT Cells(No.D202304128233)。
文摘OBJECTIVE:To investigate the therapeutic effects of Huai'er(Trametes)on psoriasis by identifying specific molecular targets and pathways involved in regulating keratinocyte behavior and immune responses.METHODS:Cellular experiments were conducted using human keratinocyte cell line(Ha Ca T)keratinocytes to evaluate the effects of Huai'er(Trametes)on cell proliferation,migration,and inflammatory factor expression.Additionally,an imiquimod(IMQ)-induced psoriasis-like mouse model was established to assess the therapeutic efficacy of Huai'er(Trametes)in vivo.Network pharmacology and transcriptomic analyses were performed to identify potential targets and pathways.RESULTS:Huai'er(Trametes)significantly inhibited Ha Ca T keratinocyte proliferation and migration in vitro,as evidenced by reduced 5-ethynyl-2′-deoxyuridine incorporation and wound healing rates.In the IMQ-induced psoriasis-like mouse model,Huai'er(Trametes)treatment reduced erythema,scaling,and dermal infiltration of inflammatory cells,demonstrating its efficacy in alleviating psoriasis symptoms.Network pharmacology and transcriptomic analyses identified signal transducer and activator of transcription 1(STAT1)as a central target modulated by Huai'er(Trametes).This regulation was associated with decreased expression of proinflammatory cytokines,including interleukin-17A and tumor necrosis factor alpha,both in vitro and in vivo.CONCLUSION:This study demonstrates that Huai'er(Trametes)exerts therapeutic effects on psoriasis by modulating STAT1,thereby inhibiting keratinocyte proliferation and inflammatory responses.The findings provide a foundation for further research into the potential of Huai'er(Trametes)as a treatment for psoriasis.
