In order to cultivate Manila clam varieties in northern China,we crossed the Dalian wild population(referred to as D)and the Weihai wild population(referred to as W)to create a 2×2 complete diallel hybrid.Ultimat...In order to cultivate Manila clam varieties in northern China,we crossed the Dalian wild population(referred to as D)and the Weihai wild population(referred to as W)to create a 2×2 complete diallel hybrid.Ultimately,we obtained two hybrid groups(D♀×W♂(DW)and W♀×D♂(WD))and two inbred groups(D♀×D♂(DD)and W♀×W♂(WW)).We measured their growth and survival during the larval and juvenile stages and compared the data between hybrid and inbred groups to study the potential heterosis of the hybrid.With the exception of slightly lower values in the WD group,fertilization and hatching rates did not differ significantly among the other groups.However,during the larval stage(days 1–15),we observed significant differences in the growth rate of Manila clam in the hybrid groups at days 1,3,and 6 of age(P<0.05).Among the groups,the clams in the DW group had the highest survival rate,which was indicative of significant heterosis.After entering the juvenile stage(days 30–120),both hybrid groups showed high heterosis in terms of growth and survival.The DW group had the greatest amount of heterosis for survival performance,and the WD group had the greatest amount of heterosis for shell length and height growth.These results indicated that this hybrid combination had great breeding potential,and provided a useful hybrid strategy for producing genetic improvements in R.philippinarum.展开更多
基金sponsored by the the Outstanding Young Scientific and Technological Talents Foundation of Dalian(No.2024RJ014)the Research Project of Liaoning Provincial Department of Education(No.LJ212410158027)+1 种基金the Research Projects of Undergraduate Universities in Liaoning Province(No.2024JBZDZ003)the Liaoning Revitalization Talents Program(No.XLYC2403165).
文摘In order to cultivate Manila clam varieties in northern China,we crossed the Dalian wild population(referred to as D)and the Weihai wild population(referred to as W)to create a 2×2 complete diallel hybrid.Ultimately,we obtained two hybrid groups(D♀×W♂(DW)and W♀×D♂(WD))and two inbred groups(D♀×D♂(DD)and W♀×W♂(WW)).We measured their growth and survival during the larval and juvenile stages and compared the data between hybrid and inbred groups to study the potential heterosis of the hybrid.With the exception of slightly lower values in the WD group,fertilization and hatching rates did not differ significantly among the other groups.However,during the larval stage(days 1–15),we observed significant differences in the growth rate of Manila clam in the hybrid groups at days 1,3,and 6 of age(P<0.05).Among the groups,the clams in the DW group had the highest survival rate,which was indicative of significant heterosis.After entering the juvenile stage(days 30–120),both hybrid groups showed high heterosis in terms of growth and survival.The DW group had the greatest amount of heterosis for survival performance,and the WD group had the greatest amount of heterosis for shell length and height growth.These results indicated that this hybrid combination had great breeding potential,and provided a useful hybrid strategy for producing genetic improvements in R.philippinarum.
