为了解决有机食品鉴别需要多种参数指标进行综合判定,制样过程繁琐,判别正确率偏低的问题,本研究提出一种基于稳定同位素比例质谱和液相色谱-高分辨质谱分析技术,结合偏最小二乘法判别分析(partial least squares-discriminant analysis...为了解决有机食品鉴别需要多种参数指标进行综合判定,制样过程繁琐,判别正确率偏低的问题,本研究提出一种基于稳定同位素比例质谱和液相色谱-高分辨质谱分析技术,结合偏最小二乘法判别分析(partial least squares-discriminant analysis,PLS-DA)的有机番茄鉴别方法。将采集的94个番茄样品(有机番茄50个,普通番茄44个)匀浆破碎、冷冻干燥后,分析并采集氮同位素比值(δ^(15)N)和液相色谱-高分辨质谱数据,应用PLS-DA方法提取数据信息,建立有机番茄判别模型,筛选并确证出其中判别的标志因子,包括氮同位素比值以及有机酸、黄酮类等13个特征化合物。所建立的模型对有机番茄的判别正确率为93.4%,基本可以满足有机番茄判别的需要。该研究为有机西红柿的快速判定提供了一种新的技术手段。展开更多
Excavated from the Western Han tomb in Tianhui Town(天回镇),Jinniu District(金牛区),Chengdu,China,Tian Hui Yi Jian(《天回医简》Tianhui Medical Slips)were penned during the Warring States period by the renowned physici...Excavated from the Western Han tomb in Tianhui Town(天回镇),Jinniu District(金牛区),Chengdu,China,Tian Hui Yi Jian(《天回医简》Tianhui Medical Slips)were penned during the Warring States period by the renowned physician Bian Que(扁鹊)and his students.Since their discovery,the medical,historical,and archaeological communities have given them considerable attention.This paper sorts out the diagnostic content of Tian Hui Yi Jian from various aspects of inspection,palpation,inquiry,listening and smelling examination and provides a summary and analysis of four features:the concept of the correspondence between heaven and man is the foundation,the idea of the five elements throughout the whole process,extremely focus on pulse diagnosis,emphasize a comprehensive analysis of four examinations.These analyses can investigate the value and significance of Tian Hui Yi Jian in developing traditional Chinese medicine(TCM)diagnosis history.In this way,we aim to reproduce the overview and level of TCM diagnostics at that time,clear up some ambiguous understanding of its development history,and provide a reference for further research on the origin and development of TCM.展开更多
该文选择天津、山东、河南3个省份具有地理标志认证的10个品种青萝卜,开展特色农产品地理来源鉴别研究。利用元素分析仪-稳定同位素质谱仪(elemental analyzer-stable isotope ratio mass spectrometers,EA-IRMS)测定了青萝卜中的碳同...该文选择天津、山东、河南3个省份具有地理标志认证的10个品种青萝卜,开展特色农产品地理来源鉴别研究。利用元素分析仪-稳定同位素质谱仪(elemental analyzer-stable isotope ratio mass spectrometers,EA-IRMS)测定了青萝卜中的碳同位素比值(δ^(13)C)、氮同位素比值(δ^(15)N),利用电感耦合等离子体质谱仪(inductively coupled plasma mass spectrometry,ICP-MS)测定了Co、Cd、As、Ni、Mo、Cr、B、Ba、Ca、Cu、Fe、Mg、Mn、Na、P、Sr、Zn、Se共18种矿物元素含量。箱线图显示,稳定同位素比值在判别产地时效果不显著;单因素ANOVA检验结果表明,Na、Ca、Mg、Fe、Sr、Zn、B、Mn、As等矿物元素含量在不同省份间存在显著差异。应用偏最小二程判别分析(partial least squares discrimination analysis,PLS-DA)算法建立判别模型时,河南、天津和山东的样品均有不同程度的误判,模型训练总体判别率为86.44%;应用线性判别分析(linear discriminant analysis,LDA)算法建模时,青萝卜样品的误判率下降,模型初始验证和交叉训练的准确率分别达到92.09%和93.79%,相对于PLS-DA模型,LDA算法建立的模型对青萝卜样品的判别准确率更高,效果更好。这说明应用LDA算法结合矿物元素对青萝卜产地鉴别具有一定可行性,也为今后应用稳定同位素比值和矿物元素含量进行萝卜产地判别提供了参考依据。展开更多
文摘为了解决有机食品鉴别需要多种参数指标进行综合判定,制样过程繁琐,判别正确率偏低的问题,本研究提出一种基于稳定同位素比例质谱和液相色谱-高分辨质谱分析技术,结合偏最小二乘法判别分析(partial least squares-discriminant analysis,PLS-DA)的有机番茄鉴别方法。将采集的94个番茄样品(有机番茄50个,普通番茄44个)匀浆破碎、冷冻干燥后,分析并采集氮同位素比值(δ^(15)N)和液相色谱-高分辨质谱数据,应用PLS-DA方法提取数据信息,建立有机番茄判别模型,筛选并确证出其中判别的标志因子,包括氮同位素比值以及有机酸、黄酮类等13个特征化合物。所建立的模型对有机番茄的判别正确率为93.4%,基本可以满足有机番茄判别的需要。该研究为有机西红柿的快速判定提供了一种新的技术手段。
基金financed by the grant from the Research Center of China Unearthed Medical Literature and Cultural Relics(No.CTYX17)。
文摘Excavated from the Western Han tomb in Tianhui Town(天回镇),Jinniu District(金牛区),Chengdu,China,Tian Hui Yi Jian(《天回医简》Tianhui Medical Slips)were penned during the Warring States period by the renowned physician Bian Que(扁鹊)and his students.Since their discovery,the medical,historical,and archaeological communities have given them considerable attention.This paper sorts out the diagnostic content of Tian Hui Yi Jian from various aspects of inspection,palpation,inquiry,listening and smelling examination and provides a summary and analysis of four features:the concept of the correspondence between heaven and man is the foundation,the idea of the five elements throughout the whole process,extremely focus on pulse diagnosis,emphasize a comprehensive analysis of four examinations.These analyses can investigate the value and significance of Tian Hui Yi Jian in developing traditional Chinese medicine(TCM)diagnosis history.In this way,we aim to reproduce the overview and level of TCM diagnostics at that time,clear up some ambiguous understanding of its development history,and provide a reference for further research on the origin and development of TCM.
文摘该文选择天津、山东、河南3个省份具有地理标志认证的10个品种青萝卜,开展特色农产品地理来源鉴别研究。利用元素分析仪-稳定同位素质谱仪(elemental analyzer-stable isotope ratio mass spectrometers,EA-IRMS)测定了青萝卜中的碳同位素比值(δ^(13)C)、氮同位素比值(δ^(15)N),利用电感耦合等离子体质谱仪(inductively coupled plasma mass spectrometry,ICP-MS)测定了Co、Cd、As、Ni、Mo、Cr、B、Ba、Ca、Cu、Fe、Mg、Mn、Na、P、Sr、Zn、Se共18种矿物元素含量。箱线图显示,稳定同位素比值在判别产地时效果不显著;单因素ANOVA检验结果表明,Na、Ca、Mg、Fe、Sr、Zn、B、Mn、As等矿物元素含量在不同省份间存在显著差异。应用偏最小二程判别分析(partial least squares discrimination analysis,PLS-DA)算法建立判别模型时,河南、天津和山东的样品均有不同程度的误判,模型训练总体判别率为86.44%;应用线性判别分析(linear discriminant analysis,LDA)算法建模时,青萝卜样品的误判率下降,模型初始验证和交叉训练的准确率分别达到92.09%和93.79%,相对于PLS-DA模型,LDA算法建立的模型对青萝卜样品的判别准确率更高,效果更好。这说明应用LDA算法结合矿物元素对青萝卜产地鉴别具有一定可行性,也为今后应用稳定同位素比值和矿物元素含量进行萝卜产地判别提供了参考依据。
