A study on balanced fertilization was conducted by means of long-term field experiments, and a convenient table for balanced fertilization was compiled. The experimental results about the effect of fertilization on wa...A study on balanced fertilization was conducted by means of long-term field experiments, and a convenient table for balanced fertilization was compiled. The experimental results about the effect of fertilization on water use efficiency of upland wheat showed that the input of inorganic fertilizer should be reduced in dry years.展开更多
目的研究中缝背核(dorsal raphe nucleus,DRN)在甲基苯丙胺(methamphetamine,METH)成瘾中的作用。方法将C57BL/6J小鼠随机分为METH模型组和对照组。通过腹腔注射METH(2mg/kg)制备条件性位置偏爱(conditioned place preference,CPP)动物...目的研究中缝背核(dorsal raphe nucleus,DRN)在甲基苯丙胺(methamphetamine,METH)成瘾中的作用。方法将C57BL/6J小鼠随机分为METH模型组和对照组。通过腹腔注射METH(2mg/kg)制备条件性位置偏爱(conditioned place preference,CPP)动物模型,对照组注射等量生理盐水。经即刻早基因c-Fos免疫组织化学染色、含Ca^(2+)指示剂病毒载体脑内注射、化学遗传学和蛋白质谱分析等技术,探究METH激活DRN脑区情况及蛋白表达变化,并分析抑制DRN脑区神经元CPP评分的变化。结果CPP评分显示,METH模型小鼠在伴药箱产生明显偏好(t=4.438,P<0.05)。METH模型组DRN内c-Fos阳性细胞数较对照组显著增多(t=4.065,P<0.05),而且DRN神经元内Ca^(2+)荧光信号明显增强(t=20.50,P<0.001)。通过叠氮平-N-氧化物(clozapine-N-oxide,CNO)腹腔注射(3mg/kg)诱导hM4Di表达抑制DRN神经元兴奋性,明显降低了METH模型组小鼠CPP分数(t=3.26,P<0.05)。蛋白质谱分析在METH模型组DRN脑区筛出了一批差异蛋白,其中突触囊泡蛋白1(Syt1)和细胞黏附分子1(Cadm1)表达升高(均P<0.05);神经束蛋白(Nfasc)和突触小泡蛋白2(SV2)的C亚型表达降低(均P<0.05)。结论DRN脑区在METH诱导小鼠CPP行为偏好中起着重要作用,METH特异性诱导DRN内与突触相关的蛋白分子的表达,可能与DRN参与的成瘾机制有关。展开更多
Objective: to investigate the preventive effect of hydrocolloid dressing on high-risk pressure ulcers in patients with stroke in convalescent period. Methods: the patients with stroke convalescence and Braden pressure...Objective: to investigate the preventive effect of hydrocolloid dressing on high-risk pressure ulcers in patients with stroke in convalescent period. Methods: the patients with stroke convalescence and Braden pressure ulcer risk assessment score ≤12 in the department of rehabilitation Medicine of our hospital from January 2019 to December 2020 were divided into experimental group and control group according to the principle of whether they agree to use water glue dressing, 40 cases in each group. Control group according to the conventional nursing prevention of pressure ulcers, such as timing turn over massage, pressure local cushion or cotton ring, cushion bed, keep local clean and dry, timely cleaning urine and feces, nutrition and support treatment, ensure the functional position of the limb, etc. On the basis of the control group, the experimental group pasted hydrocolloid dressings to protect the compression parts of the patients (such as the sacral tail, hip, shoulder blade, heel and internal and external malleolus). He occurrence of pressure ulcers was evaluated every day and observed continuously for 14 days. Results: in the control group, 8 patients developed stage I pressure ulcers, which were located at the sacral tail (8 cases), combined with heel (3 cases), lateral malleolus (1 case) and hip (1 case), and the earliest occurrence began to appear in the sacral tail on the 6th day. In the experimental group, 2 patients developed stage I pressure ulcers, which were located at the sacral tail (2 cases) and combined with the heel (1 case), and the earliest occurrence began to appear in the sacral tail on the 11th day. Two groups of patients with stage ⅰ pressure ulcers did not develop into stage II pressure ulcers after timely treatment. The total number of stage I pressure ulcers in 2 groups was significantly different (P < 0.05). Conclusion: hydrocolloid dressing has a good preventive effect on pressure ulcers in patients with high risk of pressure ulcers during the convalescent period of stroke.展开更多
文摘A study on balanced fertilization was conducted by means of long-term field experiments, and a convenient table for balanced fertilization was compiled. The experimental results about the effect of fertilization on water use efficiency of upland wheat showed that the input of inorganic fertilizer should be reduced in dry years.
文摘目的研究中缝背核(dorsal raphe nucleus,DRN)在甲基苯丙胺(methamphetamine,METH)成瘾中的作用。方法将C57BL/6J小鼠随机分为METH模型组和对照组。通过腹腔注射METH(2mg/kg)制备条件性位置偏爱(conditioned place preference,CPP)动物模型,对照组注射等量生理盐水。经即刻早基因c-Fos免疫组织化学染色、含Ca^(2+)指示剂病毒载体脑内注射、化学遗传学和蛋白质谱分析等技术,探究METH激活DRN脑区情况及蛋白表达变化,并分析抑制DRN脑区神经元CPP评分的变化。结果CPP评分显示,METH模型小鼠在伴药箱产生明显偏好(t=4.438,P<0.05)。METH模型组DRN内c-Fos阳性细胞数较对照组显著增多(t=4.065,P<0.05),而且DRN神经元内Ca^(2+)荧光信号明显增强(t=20.50,P<0.001)。通过叠氮平-N-氧化物(clozapine-N-oxide,CNO)腹腔注射(3mg/kg)诱导hM4Di表达抑制DRN神经元兴奋性,明显降低了METH模型组小鼠CPP分数(t=3.26,P<0.05)。蛋白质谱分析在METH模型组DRN脑区筛出了一批差异蛋白,其中突触囊泡蛋白1(Syt1)和细胞黏附分子1(Cadm1)表达升高(均P<0.05);神经束蛋白(Nfasc)和突触小泡蛋白2(SV2)的C亚型表达降低(均P<0.05)。结论DRN脑区在METH诱导小鼠CPP行为偏好中起着重要作用,METH特异性诱导DRN内与突触相关的蛋白分子的表达,可能与DRN参与的成瘾机制有关。
文摘Objective: to investigate the preventive effect of hydrocolloid dressing on high-risk pressure ulcers in patients with stroke in convalescent period. Methods: the patients with stroke convalescence and Braden pressure ulcer risk assessment score ≤12 in the department of rehabilitation Medicine of our hospital from January 2019 to December 2020 were divided into experimental group and control group according to the principle of whether they agree to use water glue dressing, 40 cases in each group. Control group according to the conventional nursing prevention of pressure ulcers, such as timing turn over massage, pressure local cushion or cotton ring, cushion bed, keep local clean and dry, timely cleaning urine and feces, nutrition and support treatment, ensure the functional position of the limb, etc. On the basis of the control group, the experimental group pasted hydrocolloid dressings to protect the compression parts of the patients (such as the sacral tail, hip, shoulder blade, heel and internal and external malleolus). He occurrence of pressure ulcers was evaluated every day and observed continuously for 14 days. Results: in the control group, 8 patients developed stage I pressure ulcers, which were located at the sacral tail (8 cases), combined with heel (3 cases), lateral malleolus (1 case) and hip (1 case), and the earliest occurrence began to appear in the sacral tail on the 6th day. In the experimental group, 2 patients developed stage I pressure ulcers, which were located at the sacral tail (2 cases) and combined with the heel (1 case), and the earliest occurrence began to appear in the sacral tail on the 11th day. Two groups of patients with stage ⅰ pressure ulcers did not develop into stage II pressure ulcers after timely treatment. The total number of stage I pressure ulcers in 2 groups was significantly different (P < 0.05). Conclusion: hydrocolloid dressing has a good preventive effect on pressure ulcers in patients with high risk of pressure ulcers during the convalescent period of stroke.
