目的重新评价血清定量乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)、乙型肝炎e抗原(hepatitis B e antigen,HBeAg)、乙型肝炎e抗体(antibody against HBeAg,抗-HBe)、乙型肝炎核心抗体(antibodies against hepatitis B core a...目的重新评价血清定量乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)、乙型肝炎e抗原(hepatitis B e antigen,HBeAg)、乙型肝炎e抗体(antibody against HBeAg,抗-HBe)、乙型肝炎核心抗体(antibodies against hepatitis B core antigen,抗-HBc)和乙型肝炎病毒(hepatitis B virus,HBV)DNA界定慢性HBV感染自然史分期的性能。方法本研究涉及的HBeAg阳性和阴性队列分别由245例HBeAg阳性和233例HBeAg阴性患者组成。参照Scheuer标准,HBeAg阳性和阴性病理学非显著肝炎活动(non-significant hepatitis activity,NSHA)分别定义为病理学分级≤G_(1)且分期≤S_(1)和分级≤G_(1)。根据HBV DNA预测HBeAg阳性队列病理学NSHA的反转形ROC曲线,由200例患者组成的可能HBV高复制(possibly high HBV replication,PHVR)亚队列被指定。结果HBsAg、HBeAg、抗-HBc、HBV DNA预测HBeAg阳性队列和PHVR亚队列病理学NSHA的AUC分别为0.679和0.721、0.659和0.718、0.703和0.706、0.583和0.632;HBsAg、抗-HBe、抗-HBc、HBV DNA预测HBeAg阴性队列病理学NSHA的AUC分别为0.545、0.529、0.671、0.682。ALT≤59 IU/L串联HBsAg>4.301 log_(10)IU/mL、HBeAg>2.301 log_(10)PEIU/mL预测HBeAg阳性队列病理学NSHA和分级≤G_(1)且分期≤S_(2)的正确率分别为73.9%(34/46)和95.7%(44/46)、73.9%(34/46)和93.5%(43/46);抗-HBc≤4.301 log_(10)IU/mL、HBV DNA≤3.903 log_(10)IU/mL预测HBeAg阴性队列病理学NSHA和分级≤G_(2)的正确率分别为80.0%(96/120)和92.5%(111/120)、80.5%(103/128)和92.2%(118/128)。结论ALT串联HBsAg或HBeAg可能是预测HBeAg阳性NSHA的高效参数,抗-HBc或HBV DNA可能是预测HBeAg阴性NSHA的高效参数。展开更多
Hainan Island is one of the largest islands in China and is located in the Indo-Burma biodiversity hotspot region.Despite its ecological significance,comprehensive population genetic studies of key marine organisms al...Hainan Island is one of the largest islands in China and is located in the Indo-Burma biodiversity hotspot region.Despite its ecological significance,comprehensive population genetic studies of key marine organisms along the entire coastline of Hainan Island have not been reported.This study examined the genetic diversity and population structure of the widely distributed oyster Saccostrea malabonensis around Hainan Island with analyzing mitochondrial COI gene sequences.The impacts of geographical,environmental and anthropogenic factors on genetic differentiation were also investigated.The results revealed a significant AT bias in the COI gene sequences,with transitions as the main mutation type.A total of 103 variable sites and 107 haplotypes were identified from480 COI sequences,with haplotype diversities from 0.067 to 0.782,and nucleotide diversities between 0.00011 and 0.00278.AMOVA analysis indicated that 86.65%of the variation occurred within one population while 13.35%among different populations.The average genetic distance across 16 populations was 0.00169,and the average genetic differentiation index was 0.13353.Distinct population patterns can be observed.The populations of Tonghai Village(THV)and Gangmen Mountain(GMM)in Lingshui showed similar genetic structures while those of Wanquan River Estuary(WQRE,Qionghai)and Wuzhizhou Island(WZZI,Sanya)displayed divergent evolutionary trends.Cluster analysis grouped the 480 individuals of S.malabonensis into six subpopulations.These findings are helpful for developing conservation strategies and genetic breeding programs,and are also helpful for understanding the evolutionary history of this oyster species in Hainan Island.展开更多
文摘目的重新评价血清定量乙型肝炎表面抗原(hepatitis B surface antigen,HBsAg)、乙型肝炎e抗原(hepatitis B e antigen,HBeAg)、乙型肝炎e抗体(antibody against HBeAg,抗-HBe)、乙型肝炎核心抗体(antibodies against hepatitis B core antigen,抗-HBc)和乙型肝炎病毒(hepatitis B virus,HBV)DNA界定慢性HBV感染自然史分期的性能。方法本研究涉及的HBeAg阳性和阴性队列分别由245例HBeAg阳性和233例HBeAg阴性患者组成。参照Scheuer标准,HBeAg阳性和阴性病理学非显著肝炎活动(non-significant hepatitis activity,NSHA)分别定义为病理学分级≤G_(1)且分期≤S_(1)和分级≤G_(1)。根据HBV DNA预测HBeAg阳性队列病理学NSHA的反转形ROC曲线,由200例患者组成的可能HBV高复制(possibly high HBV replication,PHVR)亚队列被指定。结果HBsAg、HBeAg、抗-HBc、HBV DNA预测HBeAg阳性队列和PHVR亚队列病理学NSHA的AUC分别为0.679和0.721、0.659和0.718、0.703和0.706、0.583和0.632;HBsAg、抗-HBe、抗-HBc、HBV DNA预测HBeAg阴性队列病理学NSHA的AUC分别为0.545、0.529、0.671、0.682。ALT≤59 IU/L串联HBsAg>4.301 log_(10)IU/mL、HBeAg>2.301 log_(10)PEIU/mL预测HBeAg阳性队列病理学NSHA和分级≤G_(1)且分期≤S_(2)的正确率分别为73.9%(34/46)和95.7%(44/46)、73.9%(34/46)和93.5%(43/46);抗-HBc≤4.301 log_(10)IU/mL、HBV DNA≤3.903 log_(10)IU/mL预测HBeAg阴性队列病理学NSHA和分级≤G_(2)的正确率分别为80.0%(96/120)和92.5%(111/120)、80.5%(103/128)和92.2%(118/128)。结论ALT串联HBsAg或HBeAg可能是预测HBeAg阳性NSHA的高效参数,抗-HBc或HBV DNA可能是预测HBeAg阴性NSHA的高效参数。
基金the Hainan Provincial Natural Science Foundation of China(No.325RC675)the Starting Research Fund from the Hainan University(No.KYQD(ZR)-21004)。
文摘Hainan Island is one of the largest islands in China and is located in the Indo-Burma biodiversity hotspot region.Despite its ecological significance,comprehensive population genetic studies of key marine organisms along the entire coastline of Hainan Island have not been reported.This study examined the genetic diversity and population structure of the widely distributed oyster Saccostrea malabonensis around Hainan Island with analyzing mitochondrial COI gene sequences.The impacts of geographical,environmental and anthropogenic factors on genetic differentiation were also investigated.The results revealed a significant AT bias in the COI gene sequences,with transitions as the main mutation type.A total of 103 variable sites and 107 haplotypes were identified from480 COI sequences,with haplotype diversities from 0.067 to 0.782,and nucleotide diversities between 0.00011 and 0.00278.AMOVA analysis indicated that 86.65%of the variation occurred within one population while 13.35%among different populations.The average genetic distance across 16 populations was 0.00169,and the average genetic differentiation index was 0.13353.Distinct population patterns can be observed.The populations of Tonghai Village(THV)and Gangmen Mountain(GMM)in Lingshui showed similar genetic structures while those of Wanquan River Estuary(WQRE,Qionghai)and Wuzhizhou Island(WZZI,Sanya)displayed divergent evolutionary trends.Cluster analysis grouped the 480 individuals of S.malabonensis into six subpopulations.These findings are helpful for developing conservation strategies and genetic breeding programs,and are also helpful for understanding the evolutionary history of this oyster species in Hainan Island.
