LIM domain proteins are found to be important regulators in cell growth, cell fate determination, cell differentiation, and remodelling of the cell cytoskeleton by their interaction with some structural proteins, kina...LIM domain proteins are found to be important regulators in cell growth, cell fate determination, cell differentiation, and remodelling of the cell cytoskeleton by their interaction with some structural proteins, kinases, transcriptional regulators, etc. The presence of LIM domains in LMCD1 gene implies it may be involved in skeletal muscle protein-protein interactions. This study was to investigate polymorphisms of LIM and cysteine-rich domain 1 （LMCD1） gene and its effect on meat quality and carcass traits in pig. The polymorphism （G294A） in exon 3 region of porcine LMCD1 gene, which is synonymous mutation, was genotyped in the population of 178 F, pigs of a Large White × Meishan resource family. Statistical results indicated the distribution of allele G （with a A → G mutation） and A （without mutation）. Analysis of variance showed that the polymorphism of LMCD1 gene was associated with variation in several carcass traits of interest for pig breeding. Some carcass traits and meat quality traits are close to significance by association. An analysis of more animals is necessary to analyze the polymorphisms in exon 3 of porcine LMCD1 gene if it was selected as a marker for the pig carcass traits.展开更多
To investigate the correlation of individual heterozygosity and heterosis of three traits in crossbred F1 pig populations, the F1 populations were built by random mating Yorkshire x Meishan （YM, n = 82）, and its rec...To investigate the correlation of individual heterozygosity and heterosis of three traits in crossbred F1 pig populations, the F1 populations were built by random mating Yorkshire x Meishan （YM, n = 82）, and its reciprocal （MY, n =47） and two straightbred populations （Yorkshire = 34, Meishan = 55） were used as control groups. The heterosis of birth weight （BWT）, average daily gain （ADG）, and feed conversion ratio （FCR） were acquired as well. In the research, the significant marker loci for the heterosis of the three traits were observed by one-way ANOVA （P〈0.01） in a total of 39 marker loci on SSC4, SSC6, SSC7, SSC8, and SSC13, and the numbers of the significant marker loci were 12 （BWT）, 18 （ADG）, and 17 （FCR）, respectively, based on which the general heterozygosity （GH） was divided into significant marker loci heterozygosity （SH） and insignificant marker loci heterozygosity （IH）. Furthermore, the trends of alteration in heterosis with the stepwise increase in heterozygosity by 0.05 were explored. This was done by the regression analysis of the three kinds of heterozygosity against heterosis of the three traits. The results showed that, for BWT, the heterosis increased with the increase in GH （r=0.9337, P=0.0021） and SH （r=0.9165, P=0.0102）; for ADG, the heterosis increased with the increase in IH （r=0.7012, P=0.0353） and GH （r=0.7470, P=0.0537, near significant）; for FCR, the heterosis of feed efficiency increased with the increase in IH （r=0.8721, P=0.0022）. The results indicated that the correlation was not always higher or more significant for SH with heterosis than it was for IH or GH with heterosis, and it might be because of the reciprocal cancellation of the positive effect and negative effect of QTL linked to the significant marker loci.展开更多
A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complet...A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complete block design and used to evaluate the influence of lairage conditions on behavior, biochemical indicators and meat quality for finishing pigs at slaughter.Behavior of the pigs was scored subjectively during lairage.Blood samples were taken at exsanguination s to measure blood temperature, plasma Cortisol,ACTH,glucose,lactate,plasma enzymes and hematological indices.Post-mortem meat quality measurements included muscle colour value(MCV),electrical conductivity(EC),pH at 45 min and 24 h from Longissimus thoracis(LM) and Semimembranosus(SM) mucles and drip loss from LM. The results showed that 3 h lairage group with toys demonstrated significantly improved behavior than the group without toys at 3 sampling times.All the pigs showed increasing calmness as the time of lairage progressed.The omission of lairage increased plasma Cortisol,ACTH,glucose and lactate(P【0.05),and decreased plasma lactate dehydrogenase(LDH),and creatine kinase(CK)(P【0.05).No biochemical index was influenced by the presence or absence of toys during lairage(P【0.05).Muscle colour value, electrical conductivity,pH at 45 min and 24 h from LM and SM and drip loss were not affected by any treatment(P【0.05).Pigs provided 3 h lairage,with or without toys,exhibited lower red blood cell(RBC), hemoglobin(HGB),and haematocrit(HCT) when compared to 0 h lairage.3 h lairage with or without toys resulted in higher white blood cell(WBC) and lymphocyte(W-SCC) levels than 0 h lairage.None of the hemocytic indices in pigs given lairage was affected by the presence or absence of toys.We conclude from this pilot study that in local commercial conditions,from the point of view of animal welfare and meat quality,lairage time of 3 h after short travel was beneficial.Pigs resting showed increased relief from stress and a recovery in immune competence.Holding pigs in lairage with toys for a few hours after arrival at the abattoir may be beneficial for the animal’s well-being.展开更多
Live measurement growth traits are very important economic traits in pig production and breeding. In this research, quantitative trait loci （QTL） were detected for 11 live estimated growth and carcass traits, includ...Live measurement growth traits are very important economic traits in pig production and breeding. In this research, quantitative trait loci （QTL） were detected for 11 live estimated growth and carcass traits, including birth weight （BWT）, average daily gain over testing periods （ADG3）, live backfat thickness at last 3-4th lumbar （LBFT3）, live loin eye area （LLEA）, and so on, in 214 pig resource family population, including 180 F2 individual, by 39 microsatellite marker loci on SSC4, SSC6, SSC7, SSC8, and SSC13. The results indicated that 4 chromosome significant level QTL and one suggestive QTL were detected for ADG3 （at position of 50 cM on SSC8）, LBFT3 （at position of 147 cM on SSC4）, LLEA （one highly significant at position of 48 cM on SSC7; another significant at position of 125 cM on SSC8） and BWT （suggestive significant at position of 0 cM, at marker sw489 on SSC4）. The phenotypic variance of these QTL accounted for 0.95% to 16.91%. Most of them were mentioned in previous reports; except the QTL of LLEA at position of sw1953 on SSC8 which maybe a new QTL.展开更多
文摘LIM domain proteins are found to be important regulators in cell growth, cell fate determination, cell differentiation, and remodelling of the cell cytoskeleton by their interaction with some structural proteins, kinases, transcriptional regulators, etc. The presence of LIM domains in LMCD1 gene implies it may be involved in skeletal muscle protein-protein interactions. This study was to investigate polymorphisms of LIM and cysteine-rich domain 1 （LMCD1） gene and its effect on meat quality and carcass traits in pig. The polymorphism （G294A） in exon 3 region of porcine LMCD1 gene, which is synonymous mutation, was genotyped in the population of 178 F, pigs of a Large White × Meishan resource family. Statistical results indicated the distribution of allele G （with a A → G mutation） and A （without mutation）. Analysis of variance showed that the polymorphism of LMCD1 gene was associated with variation in several carcass traits of interest for pig breeding. Some carcass traits and meat quality traits are close to significance by association. An analysis of more animals is necessary to analyze the polymorphisms in exon 3 of porcine LMCD1 gene if it was selected as a marker for the pig carcass traits.
基金This study was supported by grant from the National 973 Project of China （G2000016105）National High Technology Development Project （2002AA211041）the National Natural Science Foundation of China （30500358）.
文摘To investigate the correlation of individual heterozygosity and heterosis of three traits in crossbred F1 pig populations, the F1 populations were built by random mating Yorkshire x Meishan （YM, n = 82）, and its reciprocal （MY, n =47） and two straightbred populations （Yorkshire = 34, Meishan = 55） were used as control groups. The heterosis of birth weight （BWT）, average daily gain （ADG）, and feed conversion ratio （FCR） were acquired as well. In the research, the significant marker loci for the heterosis of the three traits were observed by one-way ANOVA （P〈0.01） in a total of 39 marker loci on SSC4, SSC6, SSC7, SSC8, and SSC13, and the numbers of the significant marker loci were 12 （BWT）, 18 （ADG）, and 17 （FCR）, respectively, based on which the general heterozygosity （GH） was divided into significant marker loci heterozygosity （SH） and insignificant marker loci heterozygosity （IH）. Furthermore, the trends of alteration in heterosis with the stepwise increase in heterozygosity by 0.05 were explored. This was done by the regression analysis of the three kinds of heterozygosity against heterosis of the three traits. The results showed that, for BWT, the heterosis increased with the increase in GH （r=0.9337, P=0.0021） and SH （r=0.9165, P=0.0102）; for ADG, the heterosis increased with the increase in IH （r=0.7012, P=0.0353） and GH （r=0.7470, P=0.0537, near significant）; for FCR, the heterosis of feed efficiency increased with the increase in IH （r=0.8721, P=0.0022）. The results indicated that the correlation was not always higher or more significant for SH with heterosis than it was for IH or GH with heterosis, and it might be because of the reciprocal cancellation of the positive effect and negative effect of QTL linked to the significant marker loci.
基金supported financially by the project‘Research and Development on Technology and Key Equipment for New Type of Industrialized and Healthy Animal Husbandry'funded by the Chinese Ministry of Science and Technology(2006BAD 14B02-6)11th Five Years Key Programs for Science and Technology Development of China(2006BAD01A08-07)+1 种基金Hubei Province Key Project of Science and Technology (2006AA201B24)Wuhan City Key Project of Industrialization(200720112026)
文摘A total of 60 crossbred(Large White×Landrace) pigs of halothane genotype NN(castrated males and females) were allotted to three treatments:3 h lairage with toys,3 h lairage and 0 h lairage in a randomized complete block design and used to evaluate the influence of lairage conditions on behavior, biochemical indicators and meat quality for finishing pigs at slaughter.Behavior of the pigs was scored subjectively during lairage.Blood samples were taken at exsanguination s to measure blood temperature, plasma Cortisol,ACTH,glucose,lactate,plasma enzymes and hematological indices.Post-mortem meat quality measurements included muscle colour value(MCV),electrical conductivity(EC),pH at 45 min and 24 h from Longissimus thoracis(LM) and Semimembranosus(SM) mucles and drip loss from LM. The results showed that 3 h lairage group with toys demonstrated significantly improved behavior than the group without toys at 3 sampling times.All the pigs showed increasing calmness as the time of lairage progressed.The omission of lairage increased plasma Cortisol,ACTH,glucose and lactate(P【0.05),and decreased plasma lactate dehydrogenase(LDH),and creatine kinase(CK)(P【0.05).No biochemical index was influenced by the presence or absence of toys during lairage(P【0.05).Muscle colour value, electrical conductivity,pH at 45 min and 24 h from LM and SM and drip loss were not affected by any treatment(P【0.05).Pigs provided 3 h lairage,with or without toys,exhibited lower red blood cell(RBC), hemoglobin(HGB),and haematocrit(HCT) when compared to 0 h lairage.3 h lairage with or without toys resulted in higher white blood cell(WBC) and lymphocyte(W-SCC) levels than 0 h lairage.None of the hemocytic indices in pigs given lairage was affected by the presence or absence of toys.We conclude from this pilot study that in local commercial conditions,from the point of view of animal welfare and meat quality,lairage time of 3 h after short travel was beneficial.Pigs resting showed increased relief from stress and a recovery in immune competence.Holding pigs in lairage with toys for a few hours after arrival at the abattoir may be beneficial for the animal’s well-being.
基金the National 973 Project of China （G2000016105） National Natural Science Foundation of China （30500358）.
文摘Live measurement growth traits are very important economic traits in pig production and breeding. In this research, quantitative trait loci （QTL） were detected for 11 live estimated growth and carcass traits, including birth weight （BWT）, average daily gain over testing periods （ADG3）, live backfat thickness at last 3-4th lumbar （LBFT3）, live loin eye area （LLEA）, and so on, in 214 pig resource family population, including 180 F2 individual, by 39 microsatellite marker loci on SSC4, SSC6, SSC7, SSC8, and SSC13. The results indicated that 4 chromosome significant level QTL and one suggestive QTL were detected for ADG3 （at position of 50 cM on SSC8）, LBFT3 （at position of 147 cM on SSC4）, LLEA （one highly significant at position of 48 cM on SSC7; another significant at position of 125 cM on SSC8） and BWT （suggestive significant at position of 0 cM, at marker sw489 on SSC4）. The phenotypic variance of these QTL accounted for 0.95% to 16.91%. Most of them were mentioned in previous reports; except the QTL of LLEA at position of sw1953 on SSC8 which maybe a new QTL.
