Guizhi Fuling capsule(GFC), a traditional Chinese medicine(TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various c...Guizhi Fuling capsule(GFC), a traditional Chinese medicine(TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various chemical structure types, quality control of GFC remains great challenge. In the present study, an ultra performance liquid chromatography hybrid triple-quadrupole mass spectrometry(UPLC-QQQ MS) method with ultrafast positive/negative ionization switching was developed for simultaneous determination of 18 bioactive components in GFC, including methyl gallate, ethyl gallate, oxypaeoniflorin, benzoic acid, albiflorin, paeonolide, paeoniflorin, 1, 2, 3, 4, 6-pentagalloylglucose, mudanpioside C, benzoyloxypaeoniflorin, benzoylpaeoniflorin, pachymic acid, amygdalin, cinnamaldehyde, paeonol, cinnamic acid, 4-hydroxybenzoic acid, and gallic acid. Separation was performed on an Agilent Zorbax Extend–C18 column(2.1 mm × 50 mm, 1.8 μm), using a gradient elution with acetonitrile and water containing 0.1% formic acid. Cholic acid was selected as the internal standard. This newly developed method was fully validated for linearity, precision, accuracy, and stability, and then applied to quality assessment of GFC. Finally, the batch-to-batch reproducibility of GFC samples was evaluated by the cosine ration and Euclidean distance method, which showed high quality consistency. The results demonstrated that the developed method provided a reasonable and powerful manner for quality control of GFC.展开更多
作为电池管理系统的基础,锂电池荷电状态(state of charge,SOC)的实时准确估计十分重要。现有的锂电池SOC估算方法能够实现SOC的估算,但不能评估SOC估算结果的不确定性。为了解决这个问题,将高斯过程回归(Gaussian process regression,G...作为电池管理系统的基础,锂电池荷电状态(state of charge,SOC)的实时准确估计十分重要。现有的锂电池SOC估算方法能够实现SOC的估算,但不能评估SOC估算结果的不确定性。为了解决这个问题,将高斯过程回归(Gaussian process regression,GPR)应用于锂电池SOC估算中,提出了一种基于GPR的锂电池SOC估算方法。该方法能够将电池管理系统测量所得电压、电流、温度作为输入,并以均值和置信区间的形式来输出SOC,二者可分别作为SOC的估计和不确定量化结果。另外,基于三星18650-20R锂电池数据集,分析了多种核函数对估算方法性能的影响,验证了所提方法的有效性。展开更多
鉴于锂电池荷电状态(state of charge,SOC)实时准确估计的重要性。针对大倍率脉冲放电工况下锂电池的强非线性和时变特性,训练了一个循环神经网络对锂电池SOC进行估算,该网络能够将电池管理系统测量所得的电压、电流、温度映射到SOC。...鉴于锂电池荷电状态(state of charge,SOC)实时准确估计的重要性。针对大倍率脉冲放电工况下锂电池的强非线性和时变特性,训练了一个循环神经网络对锂电池SOC进行估算,该网络能够将电池管理系统测量所得的电压、电流、温度映射到SOC。基于某型号18Ah锂电池性能测试所获得的大倍率脉冲工况放电数据进行了实验验证,得到三种温度下锂电池SOC估算结果的平均绝对值误差为1.92%,证明了该方法的有效性。展开更多
背景:近年来许多研究表明间充质干细胞所发挥的组织修复与再生功能在很大程度上是由其旁分泌作用而实现的,而外泌体是其中一种重要的旁分泌因子,已成为研究者们关注的重点。目的:阐明外泌体的组成、来源及生物学特性,总结近年来间充质...背景:近年来许多研究表明间充质干细胞所发挥的组织修复与再生功能在很大程度上是由其旁分泌作用而实现的,而外泌体是其中一种重要的旁分泌因子,已成为研究者们关注的重点。目的:阐明外泌体的组成、来源及生物学特性,总结近年来间充质干细胞来源外泌体在组织修复与再生方面的最新研究成果,并展望其在口腔领域的应用前景。方法:用计算机检索1983至2017年PubMed数据库和中国知网(CNKI)期刊全文数据库收录的相关文献。英文检索词为"exosome,MSC-exosomes,tissue repair and regeneration,oral diseases",中文检索词为"外泌体,间充质干细胞来源外泌体,组织修复与再生,口腔疾病",共纳入61篇文献。结果与结论:外泌体是细胞分泌的内含多种蛋白质及RNA等成分的小囊泡,在细胞间通讯中起重要作用,具有抑制细胞凋亡、刺激增殖、免疫调节等功能。间充质干细胞来源外泌体在骨损伤修复、皮肤损伤修复、神经损伤修复、肝损伤修复中都起到重要作用,但其作用机制还不明确,在口腔领域应用相对较少,值得深入研究和探索。展开更多
基金supported by the Specialized Research Fund for the Doctoral Program of Higher Education(No.20130096140001)the 111 Project(No.B16046)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘Guizhi Fuling capsule(GFC), a traditional Chinese medicine(TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various chemical structure types, quality control of GFC remains great challenge. In the present study, an ultra performance liquid chromatography hybrid triple-quadrupole mass spectrometry(UPLC-QQQ MS) method with ultrafast positive/negative ionization switching was developed for simultaneous determination of 18 bioactive components in GFC, including methyl gallate, ethyl gallate, oxypaeoniflorin, benzoic acid, albiflorin, paeonolide, paeoniflorin, 1, 2, 3, 4, 6-pentagalloylglucose, mudanpioside C, benzoyloxypaeoniflorin, benzoylpaeoniflorin, pachymic acid, amygdalin, cinnamaldehyde, paeonol, cinnamic acid, 4-hydroxybenzoic acid, and gallic acid. Separation was performed on an Agilent Zorbax Extend–C18 column(2.1 mm × 50 mm, 1.8 μm), using a gradient elution with acetonitrile and water containing 0.1% formic acid. Cholic acid was selected as the internal standard. This newly developed method was fully validated for linearity, precision, accuracy, and stability, and then applied to quality assessment of GFC. Finally, the batch-to-batch reproducibility of GFC samples was evaluated by the cosine ration and Euclidean distance method, which showed high quality consistency. The results demonstrated that the developed method provided a reasonable and powerful manner for quality control of GFC.
文摘作为电池管理系统的基础,锂电池荷电状态(state of charge,SOC)的实时准确估计十分重要。现有的锂电池SOC估算方法能够实现SOC的估算,但不能评估SOC估算结果的不确定性。为了解决这个问题,将高斯过程回归(Gaussian process regression,GPR)应用于锂电池SOC估算中,提出了一种基于GPR的锂电池SOC估算方法。该方法能够将电池管理系统测量所得电压、电流、温度作为输入,并以均值和置信区间的形式来输出SOC,二者可分别作为SOC的估计和不确定量化结果。另外,基于三星18650-20R锂电池数据集,分析了多种核函数对估算方法性能的影响,验证了所提方法的有效性。
文摘鉴于锂电池荷电状态(state of charge,SOC)实时准确估计的重要性。针对大倍率脉冲放电工况下锂电池的强非线性和时变特性,训练了一个循环神经网络对锂电池SOC进行估算,该网络能够将电池管理系统测量所得的电压、电流、温度映射到SOC。基于某型号18Ah锂电池性能测试所获得的大倍率脉冲工况放电数据进行了实验验证,得到三种温度下锂电池SOC估算结果的平均绝对值误差为1.92%,证明了该方法的有效性。
文摘背景:近年来许多研究表明间充质干细胞所发挥的组织修复与再生功能在很大程度上是由其旁分泌作用而实现的,而外泌体是其中一种重要的旁分泌因子,已成为研究者们关注的重点。目的:阐明外泌体的组成、来源及生物学特性,总结近年来间充质干细胞来源外泌体在组织修复与再生方面的最新研究成果,并展望其在口腔领域的应用前景。方法:用计算机检索1983至2017年PubMed数据库和中国知网(CNKI)期刊全文数据库收录的相关文献。英文检索词为"exosome,MSC-exosomes,tissue repair and regeneration,oral diseases",中文检索词为"外泌体,间充质干细胞来源外泌体,组织修复与再生,口腔疾病",共纳入61篇文献。结果与结论:外泌体是细胞分泌的内含多种蛋白质及RNA等成分的小囊泡,在细胞间通讯中起重要作用,具有抑制细胞凋亡、刺激增殖、免疫调节等功能。间充质干细胞来源外泌体在骨损伤修复、皮肤损伤修复、神经损伤修复、肝损伤修复中都起到重要作用,但其作用机制还不明确,在口腔领域应用相对较少,值得深入研究和探索。
