Astragali Radix(AR) is one of the most popular herbal medicines in traditional Chinese medicine(TCM). Wild AR is believed to be of high quality, and substitution with cultivated AR is frequently encountered in the mar...Astragali Radix(AR) is one of the most popular herbal medicines in traditional Chinese medicine(TCM). Wild AR is believed to be of high quality, and substitution with cultivated AR is frequently encountered in the market. In the present study, two types of ARs(wild and cultivated) from Astragalus membranaceus(Fisch.) Bge. and A. membranaceus var. mongholicus(Bge.) Hsiao, growing in different regions of China, were analyzed by NMR profiling coupled with multivariate analysis. Results showed that both could be differentiated successfully and cultivation patterns or growing years might have greater impact on the metabolite compositions than the variety; the metabolites responsible for the separation were identified. In addition, three extraction methods were compared and the method(M1) was used for further analysis. In M1, the extraction solvent composed of water, methanol, and chloroform in the ratio of 1 : 1 : 2 was used to obtain the aqueous methanol(upper layer) and chloroform(lower layer) fractions, respectively, showing the best separation. The differential metabolites among different methods were also revealed. Moreover, the sucrose/glucose ratio could be used as a simple index to differentiate wild and cultivated AR. Meanwhile, the changes of correlation pattern among the differential metabolites of the two varieties were found. The work demonstrated that NMR-based non-targeted profiling approach, combined with multivariate statistical analysis, can be used as a powerful tool for differentiating AR of different cultivation types or growing years.展开更多
The quality of Astragali Radix(AR) was closely related to the growth period. However, the current commodity grades of AR were only divided by diameter but not directly related to the growth period, which leads to the ...The quality of Astragali Radix(AR) was closely related to the growth period. However, the current commodity grades of AR were only divided by diameter but not directly related to the growth period, which leads to the contradiction between the grade standard and the quality evaluation index. Therefore, solving this problem will be the key for the quality evaluation of AR. The present study established a potential quality evaluation approach for the absolute growth years’ wild Astragali Radix(WAR) and transplanted Astragali Radix(TAR) based on the chemical components and anti-heart failure efficacy through adopting a bare-handed sections approach to rapidly identify the growth years of WAR. In this study, the absolute growth years of WAR were obtained by identifying the growth rings of 1–6 growth years root through the methods. The contents of flavonoids and saponins in 2–6 growth years’ WAR were determined by HPLC-UV-ELSD. The contents of 12 chemical components and the anti-fatigue failure effects of WAR(4-year-old)and TAR were compared on rat models of heart failure induced by doxorubicin. Meanwhile, NMR-based untargeted metabolomics studies were performed to investigate the regulative effects of WAR and TAR. The result shows that the numbers of growth rings were consistent with the actual growth periods of AR. The HPLC-UV-ELSD determination indicated that the content of total flavonoids in WAR was significantly higher than that in TAR. Pharmacodynamics analysis revealed that the effects of WAR on cardiac function parameters(EF, FS and LVIDs), contents of serum CK and BNP were superior to those of TAR. 13 metabolites of heart were identified that had a higher rate of change in WAR group than TAR. Overall, a rapid identification method for the growth years of WAR was established, and the fact that WAR were significantly better than TAR in the heart failure rats was first proved in the paper. This study provided a scientific basis for establishing a novel commodity specification and grade of AR for clinical rational drug use.展开更多
[ Objective] The research aimed to provide theoretical basis for further popularization and application of Berkshire Pig in Southeast Guizhou. [ Method] The carcass traits and meat quality of Berkshire（B） × Lan...[ Objective] The research aimed to provide theoretical basis for further popularization and application of Berkshire Pig in Southeast Guizhou. [ Method] The carcass traits and meat quality of Berkshire（B） × Landrace（L） × Yorkshire（Y）, Duroc（D） × Landrace（L） x Yorkshire（Y） and Lanclrace （L） xYorkshire（Y） were determined by slaughter test. And the application effect of Berkshire in the minority areas was analyzed. [Result] From the carcass traits, the hybridized pig of Berkshire（B） x Landrace（L） ×Yorkshire（Y） had higher slaughter rate and lower lean meat percentage, having extremely significant difference with Landrace （L） × Yorkshire （Y） and significant difference with Durec （D） × Landrace （L） × Yorkshire（Y）. The meat indices of Berkshire（B） × Landrace（L） ×Yorkshire（Y） were better than that of Landrace （L） ×Yorkshire（Y） and Duroc （D）× Landrace（L） ×Yorkshire（Y）. The eye muscle area of Berkshire（B） ×Landrace（L） xYorkshire（Y） had extremely significant difference with Landrace （L） × Yorkshire（Y） and significant difference with Duroc（D） × Landrace（L） × Yorkshire（Y）. The meat color, marbling and cocking percentage and other meat indices were similar with that of Jianhe White Xiang Pig, Nayong Nuogu Pig and Cola Pig. [ Conclusion] Berkshire（B） × Landrace（L） xYorkshire（Y） was more suiTab, for residents in the minority mountain areas, so it was worthy to be popularized.展开更多
This dissertation aims at providing steady sensing for the shape detection of colonoscopes. The research especially deals with the key techniques of fiber bragg grating (FBG) large curvature sensor and sensor net, int...This dissertation aims at providing steady sensing for the shape detection of colonoscopes. The research especially deals with the key techniques of fiber bragg grating (FBG) large curvature sensor and sensor net, integrates the techniques of mechatronics and computer graphics, and develops real time FBG shape sensing system and incremental shape sensing system for colonoscopies.展开更多
基金supported by the Ministry of Agriculture for providing New Application for Herbal Research Grant Scheme(NRGS)(No.NH1014D040)the National 12th 5-Year Science and Technology Support Program(No.2011BA107B01)+1 种基金the Science and Technology Innovation Team of Shanxi Province(No.2013131015)the National Natural Science Foundation of China(No.31570346)
文摘Astragali Radix(AR) is one of the most popular herbal medicines in traditional Chinese medicine(TCM). Wild AR is believed to be of high quality, and substitution with cultivated AR is frequently encountered in the market. In the present study, two types of ARs(wild and cultivated) from Astragalus membranaceus(Fisch.) Bge. and A. membranaceus var. mongholicus(Bge.) Hsiao, growing in different regions of China, were analyzed by NMR profiling coupled with multivariate analysis. Results showed that both could be differentiated successfully and cultivation patterns or growing years might have greater impact on the metabolite compositions than the variety; the metabolites responsible for the separation were identified. In addition, three extraction methods were compared and the method(M1) was used for further analysis. In M1, the extraction solvent composed of water, methanol, and chloroform in the ratio of 1 : 1 : 2 was used to obtain the aqueous methanol(upper layer) and chloroform(lower layer) fractions, respectively, showing the best separation. The differential metabolites among different methods were also revealed. Moreover, the sucrose/glucose ratio could be used as a simple index to differentiate wild and cultivated AR. Meanwhile, the changes of correlation pattern among the differential metabolites of the two varieties were found. The work demonstrated that NMR-based non-targeted profiling approach, combined with multivariate statistical analysis, can be used as a powerful tool for differentiating AR of different cultivation types or growing years.
基金the National Key R&D Program of China(No.2019YFC1710800)China Postdoctoral Science Foundation Project(No.2019M650851)+3 种基金the National Natural Science Foundation of China(No.81872962)Science and Technology Research Project of Shanxi Province(No.20150313004-5)Key Projects of Key Research and Development Plan in Shanxi(No.201603D311101)Shanxi Province Technology Innovation Project of Excellent Talent(Nos.201605D211030,201705D211020)。
文摘The quality of Astragali Radix(AR) was closely related to the growth period. However, the current commodity grades of AR were only divided by diameter but not directly related to the growth period, which leads to the contradiction between the grade standard and the quality evaluation index. Therefore, solving this problem will be the key for the quality evaluation of AR. The present study established a potential quality evaluation approach for the absolute growth years’ wild Astragali Radix(WAR) and transplanted Astragali Radix(TAR) based on the chemical components and anti-heart failure efficacy through adopting a bare-handed sections approach to rapidly identify the growth years of WAR. In this study, the absolute growth years of WAR were obtained by identifying the growth rings of 1–6 growth years root through the methods. The contents of flavonoids and saponins in 2–6 growth years’ WAR were determined by HPLC-UV-ELSD. The contents of 12 chemical components and the anti-fatigue failure effects of WAR(4-year-old)and TAR were compared on rat models of heart failure induced by doxorubicin. Meanwhile, NMR-based untargeted metabolomics studies were performed to investigate the regulative effects of WAR and TAR. The result shows that the numbers of growth rings were consistent with the actual growth periods of AR. The HPLC-UV-ELSD determination indicated that the content of total flavonoids in WAR was significantly higher than that in TAR. Pharmacodynamics analysis revealed that the effects of WAR on cardiac function parameters(EF, FS and LVIDs), contents of serum CK and BNP were superior to those of TAR. 13 metabolites of heart were identified that had a higher rate of change in WAR group than TAR. Overall, a rapid identification method for the growth years of WAR was established, and the fact that WAR were significantly better than TAR in the heart failure rats was first proved in the paper. This study provided a scientific basis for establishing a novel commodity specification and grade of AR for clinical rational drug use.
基金Supported by Key Project by Department of Science and Technology in Guizhou Province(Qiankehe 2011-5020)
文摘[ Objective] The research aimed to provide theoretical basis for further popularization and application of Berkshire Pig in Southeast Guizhou. [ Method] The carcass traits and meat quality of Berkshire（B） × Landrace（L） × Yorkshire（Y）, Duroc（D） × Landrace（L） x Yorkshire（Y） and Lanclrace （L） xYorkshire（Y） were determined by slaughter test. And the application effect of Berkshire in the minority areas was analyzed. [Result] From the carcass traits, the hybridized pig of Berkshire（B） x Landrace（L） ×Yorkshire（Y） had higher slaughter rate and lower lean meat percentage, having extremely significant difference with Landrace （L） × Yorkshire （Y） and significant difference with Durec （D） × Landrace （L） × Yorkshire（Y）. The meat indices of Berkshire（B） × Landrace（L） ×Yorkshire（Y） were better than that of Landrace （L） ×Yorkshire（Y） and Duroc （D）× Landrace（L） ×Yorkshire（Y）. The eye muscle area of Berkshire（B） ×Landrace（L） xYorkshire（Y） had extremely significant difference with Landrace （L） × Yorkshire（Y） and significant difference with Duroc（D） × Landrace（L） × Yorkshire（Y）. The meat color, marbling and cocking percentage and other meat indices were similar with that of Jianhe White Xiang Pig, Nayong Nuogu Pig and Cola Pig. [ Conclusion] Berkshire（B） × Landrace（L） xYorkshire（Y） was more suiTab, for residents in the minority mountain areas, so it was worthy to be popularized.
文摘This dissertation aims at providing steady sensing for the shape detection of colonoscopes. The research especially deals with the key techniques of fiber bragg grating (FBG) large curvature sensor and sensor net, integrates the techniques of mechatronics and computer graphics, and develops real time FBG shape sensing system and incremental shape sensing system for colonoscopies.
