To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis.The mutant showed a stable rolled-leaf phenotype throughout the life cycle...To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis.The mutant showed a stable rolled-leaf phenotype throughout the life cycle.Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64.Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene.To determine the location of the gene,bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7.The rolled-leaf gene,tentatively named rl11(t),is likely a new gene as no other rolled-leaf genes have been identified near the region.By developing new SSR and InDel markers,the gene was delimited to a 52 kb region near the end of the short chromosome arm.Further fine mapping and cloning of the gene are currently underway.展开更多
基金Supported by the National High Technology Research and Development Program of China(Grant Nos.2006AA10Z1E8 and 2006AA100101)
文摘To understand the development of rice leaf blades,we identified a new rolled-leaf mutant,w32,from indica cultivar IR64 through EMS mutagenesis.The mutant showed a stable rolled-leaf phenotype throughout the life cycle.Two F2 populations were developed by crossing w32 to cultivar IR24 and PA64.Genetic analysis showed that the rolled-leaf phenotype was controlled by a single recessive gene.To determine the location of the gene,bulked segregant analysis was carried out using mutant and wild-type DNA pools and 1846 mutant-type F2 individuals derived from the cross w32/PA64 were genotyped to locate the gene on the short arm of chromosome 7.The rolled-leaf gene,tentatively named rl11(t),is likely a new gene as no other rolled-leaf genes have been identified near the region.By developing new SSR and InDel markers,the gene was delimited to a 52 kb region near the end of the short chromosome arm.Further fine mapping and cloning of the gene are currently underway.
