AIM: To better clarify the main target organs of dimethylarsinic acid toxicity and the role of metallothionein (MTs) in modifying dimethylarsinic acid (DMAA) toxicity. METHODS: MT-Ⅰ/Ⅱ null (MT^-/-) mice and the corr...AIM: To better clarify the main target organs of dimethylarsinic acid toxicity and the role of metallothionein (MTs) in modifying dimethylarsinic acid (DMAA) toxicity. METHODS: MT-Ⅰ/Ⅱ null (MT^-/-) mice and the corresponding wild-type mice (MT^+/+), six in each group, were exposed to DMAA (0-750 mg/kg body weight) by a single oral injection.Twenty four hours later, the lungs, livers and kidneys were collected and undergone pathological analysis, induction of apoptolic cells as determined by TUNEL and Mr concentration was detected by radio-immunoassay. RESULTS: Remarkable pathological lesions were observed at the doses ranging from 350 to 750 mg/kg body weight in the lungs, livers and kidneys and MT^+/+ mice exhibited a relatively slight destruction when compared with that in dose matched MT/ mice. The number of apoptotic cells was increased in a dose dependent manner in the lungs and livers in both types of mice. DMAA produced more necrotic cells rather than apoptotic cells at the highest dose of 750 mg/kg,however, no significant increase was observed in the kidney.Hepatic Mr level in Mr^+/+ mice was significantly increased by DMAA in a dose-dependent manner and there was no detectable amount of hepatic MT in untreated MT^-/- mice. CONCLUSION: DMAA treatment can lead to the induction of apoptosis and pathological damage in both types of mice.MT exhibits a protective effect against DMAA toxicity.展开更多
In order to do a good job in the treatment and nursing of critically ill patients in ICU, the application effect of invasive arterial blood pressure monitoring method was investigated. This time, a total of 80 patient...In order to do a good job in the treatment and nursing of critically ill patients in ICU, the application effect of invasive arterial blood pressure monitoring method was investigated. This time, a total of 80 patients from May 2020 to May 2022 in a hospital were selected and reasonably divided into a control group and an observation group with the same number of patients. Patients in the control group were treated by invasive arterial blood pressure monitoring through radial artery puncture, and routine care was taken. On this basis, patients in the observation group were treated and studied by invasive arterial blood pressure monitoring through dorsum pedis artery puncture, and comprehensive care measures were taken. After a period of time, the two groups were compared in terms of complications, arterial cannula indwelling time and other aspects. Standing on the basis of one-time puncture operation, the observation composition power is significantly higher than that of the control group, and there is no hidden danger of various indwelling complications. In the observation group, the patients arterial cannula is indwelled for a long time, and all aspects are better than that of the control group, and there is significant difference in data comparison between the groups. It can be seen from this that, in order to do a good job in the treatment and nursing of critically ill patients in ICU, it is extremely critical to adopt invasive arterial blood pressure monitoring method through dorsal artery puncture. Compared with invasive arterial blood pressure monitoring method through radial artery puncture, it can control the occurrence of various complications as much as possible, at the same time, it can also improve the treatment effectiveness of patients, and fully increase the retention time of arterial cannula. It is worthy of all-round promotion and application in industry and life.展开更多
文摘AIM: To better clarify the main target organs of dimethylarsinic acid toxicity and the role of metallothionein (MTs) in modifying dimethylarsinic acid (DMAA) toxicity. METHODS: MT-Ⅰ/Ⅱ null (MT^-/-) mice and the corresponding wild-type mice (MT^+/+), six in each group, were exposed to DMAA (0-750 mg/kg body weight) by a single oral injection.Twenty four hours later, the lungs, livers and kidneys were collected and undergone pathological analysis, induction of apoptolic cells as determined by TUNEL and Mr concentration was detected by radio-immunoassay. RESULTS: Remarkable pathological lesions were observed at the doses ranging from 350 to 750 mg/kg body weight in the lungs, livers and kidneys and MT^+/+ mice exhibited a relatively slight destruction when compared with that in dose matched MT/ mice. The number of apoptotic cells was increased in a dose dependent manner in the lungs and livers in both types of mice. DMAA produced more necrotic cells rather than apoptotic cells at the highest dose of 750 mg/kg,however, no significant increase was observed in the kidney.Hepatic Mr level in Mr^+/+ mice was significantly increased by DMAA in a dose-dependent manner and there was no detectable amount of hepatic MT in untreated MT^-/- mice. CONCLUSION: DMAA treatment can lead to the induction of apoptosis and pathological damage in both types of mice.MT exhibits a protective effect against DMAA toxicity.
文摘In order to do a good job in the treatment and nursing of critically ill patients in ICU, the application effect of invasive arterial blood pressure monitoring method was investigated. This time, a total of 80 patients from May 2020 to May 2022 in a hospital were selected and reasonably divided into a control group and an observation group with the same number of patients. Patients in the control group were treated by invasive arterial blood pressure monitoring through radial artery puncture, and routine care was taken. On this basis, patients in the observation group were treated and studied by invasive arterial blood pressure monitoring through dorsum pedis artery puncture, and comprehensive care measures were taken. After a period of time, the two groups were compared in terms of complications, arterial cannula indwelling time and other aspects. Standing on the basis of one-time puncture operation, the observation composition power is significantly higher than that of the control group, and there is no hidden danger of various indwelling complications. In the observation group, the patients arterial cannula is indwelled for a long time, and all aspects are better than that of the control group, and there is significant difference in data comparison between the groups. It can be seen from this that, in order to do a good job in the treatment and nursing of critically ill patients in ICU, it is extremely critical to adopt invasive arterial blood pressure monitoring method through dorsal artery puncture. Compared with invasive arterial blood pressure monitoring method through radial artery puncture, it can control the occurrence of various complications as much as possible, at the same time, it can also improve the treatment effectiveness of patients, and fully increase the retention time of arterial cannula. It is worthy of all-round promotion and application in industry and life.
