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Alternative splicing of ZmHsf23 modulates maize heat tolerance by regulating sHSPs and TIL1 expression
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作者 Jing Wang Nannan Song +6 位作者 Qianqian Qin Anqi Su Weina Si Beijiu Cheng leiming wu Xiaojian Peng Haiyang Jiang 《The Crop Journal》 2025年第4期1041-1053,共13页
Heat stress is a major threat to maize(Zea mays L.)production worldwide.Heat shock transcription factors(HSFs)play vital roles in plant responses to heat stress.However,the molecular and genetic mechanisms underlying ... Heat stress is a major threat to maize(Zea mays L.)production worldwide.Heat shock transcription factors(HSFs)play vital roles in plant responses to heat stress.However,the molecular and genetic mechanisms underlying HSF-meditated thermotolerance in maize remain largely unexplored.In this study,we demonstrate that the alternative splicing of ZmHsf23 modulates heat stress tolerance in maize.Hsf23 produced two functional transcripts,Hsf23L and Hsf23S,which differ by the presence of a cryptic mini-exon in Hsf23L that is spliced out in Hsf23S.Both transcripts were strongly induced by heat stress.Mutants lacking Hsf23L alone(hsf23l)or both Hsf23L and Hsf23S(hsf23l23s)exhibited increased susceptibility to heat stress,whereas overexpression of Hsf23S enhanced heat stress tolerance in maize.Subsequently,we found that Hsf23S positively regulates heat stress tolerance by directly activating the transcription of three sHSP genes(Hsp16.9,Hsp17.2,and Hsp18a)and TIL1 gene.In addition,Hsf23L physically interacted with Hsf23S and enhanced the transcriptional activation of Hsf23S on the sHSPs and TIL1 promoters.Notably,genetic analysis suggested that co-overexpression of Hsf23L and Hsf23S further improves heat tolerance of the transgenic plants.Taken together,these results reveal two splicing variants of ZmHsf23 cooperatively regulate maize heat tolerance,thus highlighting potential value of ZmHsf23 in breeding heat-tolerant maize varieties. 展开更多
关键词 Heat stress ZmHsf23 Alternative splicing Heat stress tolerance MAIZE
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The ZmHSF08-ZmUGT92A1 module regulates heat tolerance by altering reactive oxygen species levels in maize 被引量:1
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作者 Hao Li Jing Wang +4 位作者 Menglong Li leiming wu Wenhui Rao Xiaojian Peng Haiyang Jiang 《The Crop Journal》 SCIE CSCD 2024年第5期1437-1446,共10页
GTs(Glycosyltransferases)are important in plant growth and abiotic stresses.However,its role in maize heat response is far from clear.Here,we describe the constitutively expressed UDP-glycosyltransferase ZmUGT92A1,whi... GTs(Glycosyltransferases)are important in plant growth and abiotic stresses.However,its role in maize heat response is far from clear.Here,we describe the constitutively expressed UDP-glycosyltransferase ZmUGT92A1,which has a highly conserved PSPG box and is localized in chloroplasts,is induced under heat stress.Functional disruption of ZmUGT92A1 leads to heat sensitivity and reactive oxygen species accumulation in maize.Metabolomics analysis revealed that ZmUGT92A1 affected multiple metabolic pathways and altered the metabolic homeostasis of flavonoids under heat stress.In vitro assay showed ZmUGT92A1 exhibits glycosyltransferase activity on flavonoids and hormones.Additionally,we identified a rapidly heat-induced transcription factor,ZmHSF08,which can directly bind and repress the promoter region of ZmUGT92A1.The ZmHSF08 overexpression line exhibits heat sensitivity and reactive oxygen species accumulation.These findings reveal that the ZmHSF08-ZmUGT92A1 module plays a role in heat tolerance in maize and provide candidate strategies for the development of heat-tolerant varieties. 展开更多
关键词 Heat shock transcription factors Heat stress Oxidative damage Uridine diphosphate glycosyltransferase Zea mays L.
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云纹石斑鱼、七带石斑鱼及杂交F1肌肉营养成分分析 被引量:2
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作者 李炎璐 陈超 +3 位作者 吴雷明 宋振鑫 林利民 翟介明 《水产研究》 2016年第2期11-18,共8页
为确定并比较云纹石斑鱼(Epinephelus moara)、七带石斑鱼(Epinephelus septemfasciatus)及其云纹石斑鱼(Epinephelus moara)♀ &#215;七带石斑鱼(Epinephelus septemfasciatus)♂杂交F1的营养成分及差异,对3种石斑鱼的6月龄幼鱼进... 为确定并比较云纹石斑鱼(Epinephelus moara)、七带石斑鱼(Epinephelus septemfasciatus)及其云纹石斑鱼(Epinephelus moara)♀ &#215;七带石斑鱼(Epinephelus septemfasciatus)♂杂交F1的营养成分及差异,对3种石斑鱼的6月龄幼鱼进行肌肉营养成分、氨基酸、脂肪酸、矿物质和微量元素的组成及含量分析。结果表明,七带石斑鱼粗蛋白(17.40%)和粗脂肪(5.40%)含量最高,杂交F1水分(77.00%)含量最高;七带石斑鱼肌肉中总氨基酸(13.11%)含量、必须氨基酸(4.88%)含量以及鲜味氨基酸(4.13%)含量均最高,并且比例最为适宜;3种石斑鱼多不饱和脂肪酸(PUFA)与饱和脂肪酸(SFA)比例均符合理想脂肪酸的标准,其中杂交F1的多不饱和脂肪酸(33.50%)含量最高,并且EPA和DHA(16.13%)含量最高,七带石斑鱼的单不饱和脂肪酸(36.23%)含量最高;七带石斑鱼的铜、钙、钾、磷、镁含量最高,杂交F1的铁、钠含量最高,云纹石斑鱼锌含量最高。研究表明,3种石斑鱼中,七带石斑鱼是最为优质的蛋白源,杂交F1和七带石斑鱼中不饱和脂肪酸含量高,杂交F1多不饱和脂肪酸含量具有明显优势,具有较高的营养和经济价值。 展开更多
关键词 云纹石斑鱼 七带石斑鱼 杂交F1 营养成分 氨基酸 脂肪酸
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All-optical logic devices based on black arsenic-phosphorus with strong nonlinear optical response and high stability 被引量:4
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作者 leiming wu Taojian Fan +10 位作者 Songrui Wei Yijun Xu Ye Zhang Dingtao Ma Yiqing Shu Yuanjiang Xiang Jun Liu Jianqing Li Krassimir Panajotov Yuwen Qin Han Zhang 《Opto-Electronic Advances》 SCIE EI 2022年第1期13-23,共11页
The Kerr nonlinearity in two-dimensional(2D)nanomaterials is emerging as an appealing and intriguing research area due to their prominent light processing,modulation,and manipulation abilities.In this contribution,2D ... The Kerr nonlinearity in two-dimensional(2D)nanomaterials is emerging as an appealing and intriguing research area due to their prominent light processing,modulation,and manipulation abilities.In this contribution,2D black arsenic-phosphorus(B-AsP)nanosheets(NSs)were applied in nonlinear photonic devices based on spatial self-phase modula-tion(SSPM)method.By applying the Kerr nonlinearity in 2D B-AsP,an all-optical phase-modulated system is proposed to realize the functions of“on”and“off”in all-optical switching.By using the same all-optical phase-modulated system,another optical logic gate is proposed,and the logical“or”function is obtained based on the 2D B-AsP NSs dispersions.Moreover,by using the SSPM method,a 2D B-AsP/SnS_(2) hybrid structure is fabricated,and the result illustrates that the hybrid structure possesses the ability of the unidirectional nonlinear excitation,which helps in obtaining the function of spatial asymmetric light propagation.This function is considered an important prerequisite for the realization of diode functionalization,which is believed to be a factor in important basis for the design of isolators as well.The initial investig-ations indicate that 2D B-AsP is applicable for designing optical logical devices,which can be considered as an import-ant development in all-optical information processing. 展开更多
关键词 B-AsP nanomaterial all-optical phase-modulated system spatial asymmetric light propagation
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The complex transcriptional regulation of heat stress response in maize 被引量:1
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作者 Mingxiu Ruan Heng Zhao +7 位作者 Yujing Wen Hao Chen Feng He Xingbo Hou Xiaoqin Song Haiyang Jiang Yong-Ling Ruan leiming wu 《Stress Biology》 2024年第1期465-478,共14页
As one of the most important food and feed crops worldwide,maize suffers much more tremendous damages under heat stress compared to other plants,which seriously inhibits plant growth and reduces productivity.To mitiga... As one of the most important food and feed crops worldwide,maize suffers much more tremendous damages under heat stress compared to other plants,which seriously inhibits plant growth and reduces productivity.To mitigate the heat-induced damages and adapt to high temperature environment,plants have evolved a series of molecular mechanisms to sense,respond and adapt high temperatures and heat stress.In this review,we summarized recent advances in molecular regulations underlying high temperature sensing,heat stress response and memory in maize,especially focusing on several important pathways and signals in high temperature sensing,and the complex transcriptional regulation of ZmHSFs(Heat Shock Factors)in heat stress response.In addition,we highlighted interactions between ZmHSFs and several epigenetic regulation factors in coordinately regulating heat stress response and memory.Finally,we laid out strategies to systematically elucidate the regulatory network of maize heat stress response,and discussed approaches for breeding future heat-tolerance maize. 展开更多
关键词 Heat stress MAIZE Transcriptional regulation Epigenetic regulation INTERACTION
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Observation of spatiotemporal dynamics for topological surface states with on-demand dispersion
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作者 ZHANYUAN ZHANG ZHIHAO LAN +5 位作者 PENGBAI XU leiming wu MENGLIN L.N.CHEN WEI E.I.SHA YI XU YUWEN QIN 《Photonics Research》 CSCD 2024年第12期2919-2930,共12页
Dispersion management in guided wave optics is of vital importance for various applications.Topological photonics opens new horizons for manipulating unidirectional guided waves utilizing edge states.However,the exper... Dispersion management in guided wave optics is of vital importance for various applications.Topological photonics opens new horizons for manipulating unidirectional guided waves utilizing edge states.However,the experimental observation of spatiotemporal dynamics for guided waves with on-demand dispersion in topological photonic crystal is an important issue awaiting exploitation.Herein,we experimentally investigate the spatiotemporal properties of topological surface states with on-demand dispersion,where they are supported by a truncated valley photonic crystal with surface modulation.We observe the electromagnetic dynamics of surface states with typical dispersions,where dynamical trapping of an electromagnetic pulse mediated by the unidirectional surface state with flat dispersion and backward beam routing using reversed dispersion properties are achieved in photonic crystal slabs.Both numerical and experimental results substantiate the ultimate dispersion management for topological surface states,which could pave new ways for the manipulation of electromagnetic waves on the surface of photonic devices. 展开更多
关键词 DISPERSION SURFACE DYNAMICS
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Using Interactome Big Data to Crack Genetic Mysteries and Enhance Future Crop Breeding 被引量:10
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作者 leiming wu Linqian Han +3 位作者 Qing Li Guoying Wang Hongwei Zhang Lin Li 《Molecular Plant》 SCIE CAS CSCD 2021年第1期77-94,共18页
The functional genes underlying phenotypic variation and their interactions represent“genetic mysteries”.Understanding and utilizing these genetic mysteries are key solutions for mitigating the current threats to ag... The functional genes underlying phenotypic variation and their interactions represent“genetic mysteries”.Understanding and utilizing these genetic mysteries are key solutions for mitigating the current threats to agriculture posed by population growth and individual food preferences.Due to advances in highthroughput multi-omics technologies,we are stepping into an Interactome Big Data era that is certain to revolutionize genetic research.In this article,we provide a brief overview of current strategies to explore genetic mysteries.We then introduce the methods for constructing and analyzing the Interactome Big Data and summarize currently available interactome resources.Next,we discuss how Interactome Big Data can be used as a versatile tool to dissect genetic mysteries.We propose an integrated strategy that could revolutionize genetic research by combining Interactome Big Data with machine learning,which involves mining information hidden in Big Data to identify the genetic models or networks that control various traits,and also provide a detailed procedure for systematic dissection of genetic mysteries,Finally,we discuss three promising future breeding strategies utilizing the Interactome Big Data to improve crop yields and quality. 展开更多
关键词 genetic mystery interactome big data machine learning crop breeding
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Hic74,a novel alanine and glycine rich matrix protein related to nacreous layer formation in the mollusc Hyriopsis cumingii 被引量:8
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作者 Xiaojun Liu Can Jin +3 位作者 leiming wu Shaojian Dong Shimei Zeng Jiale Li 《Aquaculture and Fisheries》 2017年第3期119-123,共5页
Organic matrix proteins play an important role in the biomineralization of mollusc shells.We have identified and characterized a novel matrix protein,namely hic74,isolated from the mantle of the freshwater mussel Hyri... Organic matrix proteins play an important role in the biomineralization of mollusc shells.We have identified and characterized a novel matrix protein,namely hic74,isolated from the mantle of the freshwater mussel Hyriopsis cumingii.The hic74 gene encodes a 850 amino acid protein that is rich in alanine(ala,30.8%),glycine(gly,25.8%)and serine(ser,10.6%),with ala mainly existing in poly-ala forms within the ala/gly-rich regions.Quantitative PCR expression analysis demonstrated that hic74 was specifically amplified from the mantle and in situ hybridization showed a strong signal in the epithelial cells in the pallial region of the mantle.Lower levels of expression were detected during the early stages of pearl sac formation but increased and remained constant during nacreous layer formation suggesting that hic74 might be involved in this process. 展开更多
关键词 Hyriopsis cumingii Freshwater mussel Matrix protein PEARL BIOMINERALIZATION
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MXene sensors based on optical and electrical sensing signals:from biological,chemical,and physical sensing to emerging intelligent and bionic devices 被引量:2
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作者 leiming wu Xixi Yuan +7 位作者 Yuxuan Tang S.Wageh Omar A.Al-Hartomy Abdullah G.Al-Sehemi Jun Yang Yuanjiang Xiang Han Zhang Yuwen Qin 《PhotoniX》 SCIE EI 2023年第1期438-493,共56页
Sensing devices are key nodes for information detection,processing,and conversion and are widely applied in different fields such as industrial production,environmental monitoring,and defense.However,increasing demand... Sensing devices are key nodes for information detection,processing,and conversion and are widely applied in different fields such as industrial production,environmental monitoring,and defense.However,increasing demand of these devices has complicated the application scenarios and diversified the detection targets thereby promoting the continuous development of sensing materials and detection methods.In recent years,Tin+1CnTx(n=1,2,3)MXenes with outstanding optical,electrical,thermal,and mechanical properties have been developed as ideal candidates of sensing materials to apply in physical,chemical,and biological sensing fields.In this review,depending on optical and electrical sensing signals,we systematically summarize the application of Tin+1CnTx in nine categories of sensors such as strain,gas,and fluorescence sensors.The excellent sensing properties of Tin+1CnTx allow its further development in emerging intelligent and bionic devices,including smart flexible devices,bionic E-skin,neural network coding and learning,bionic soft robot,as well as intelligent artificial eardrum,which are all discussed briefly in this review.Finally,we present a positive outlook on the potential future challenges and perspectives of MXene-based sensors.MXenes have shown a vigorous development momentum in sensing applications and can drive the development of an increasing number of new technologies. 展开更多
关键词 Tin+1CnTx(n=1 2 3)MXenes Sensing applications Intelligent devices
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Ultrasensitive biosensors based on long-range surface plasmon polariton and dielectric waveguide modes 被引量:4
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作者 leiming wu Jun Guo +2 位作者 Hailin Xu Xiaoyu Dai Yuanjiang Xiang 《Photonics Research》 SCIE EI 2016年第6期262-266,共5页
An ultrasensitive biosensor based on hybrid structure and composed of long-range surface plasmon polariton(LRSPP) and dielectric planar waveguide(PWG) modes is proposed. Both PWG and LRSPP modes have strong resonances... An ultrasensitive biosensor based on hybrid structure and composed of long-range surface plasmon polariton(LRSPP) and dielectric planar waveguide(PWG) modes is proposed. Both PWG and LRSPP modes have strong resonances to form strong coupling between the two modes, and the two modes can couple to enhance sensitivity of sensors. In the hybrid structure, PWG is composed of cytop–Si–cytop multilayers and the LRSPP configuration is composed of cytop–metal–sensing medium multilayer slabs. The highest imaging sensitivities of 2264 and3619 RIU-1were realized in the proposed sensors based on Au and Al-monolayer graphene, respectively, which are nearly 1.2 and 1.9 times larger than the 1910 RIU-1sensitivity of the conventional LRSPR sensor(LRSPP sensor). Moreover, it is demonstrated that the PWG-coupled LRSPP biosensor is applicable to the sensing medium,with refractive index in the vicinity of 1.34. 展开更多
关键词 RIU Ultrasensitive biosensors based on long-range surface plasmon polariton and dielectric waveguide modes
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A cost-effective ts CUT&Tag method for profiling transcription factor binding landscape 被引量:1
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作者 leiming wu Zi Luo +10 位作者 Yanni Shi Yizhe Jiang Ruonan Li Xinxin Miao Fang Yang Qing Li Han Zhao Jiquan Xue Shutu Xu Tifu Zhang Lin Li 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2022年第11期2033-2038,共6页
Knowledge of the transcription factor binding landscape(TFBL)is necessary to analyze gene regulatory networks for important agronomic traits.However,a low-cost and high-throughput in vivo chromatin profiling method is... Knowledge of the transcription factor binding landscape(TFBL)is necessary to analyze gene regulatory networks for important agronomic traits.However,a low-cost and high-throughput in vivo chromatin profiling method is still lacking in plants.Here,we developed a transient and simplified cleavage under targets and tagmentation(tsCUT&Tag)that combines transient expression of transcription factor proteins in protoplasts with a simplified CUT&Tag without nucleus extraction.Our tsCUT&Tag method provided higher data quality and signal resolution with lower sequencing depth compared with traditional ChIP-seq.Furthermore,we developed a strategy combining tsCUT&Tag with machine learning,which has great potential for profiling the TFBL across plant development. 展开更多
关键词 binding sites CHIP-SEQ CUT&Tag machine learning transcription factor transient expression
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Broadband nonlinear optical resonance and all-optical switching of liquid phase exfoliated tungsten diselenide 被引量:2
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作者 YUE JIA YOUXIAN SHAN +3 位作者 leiming wu XIAOYU DAI DIANYUAN FAN YUANJIANG XIANG 《Photonics Research》 SCIE EI 2018年第11期1040-1047,共8页
As a kind of two-dimensional transition metal dichalcogenide material, tungsten diselenide(WSe_2) has attracted increasing attention, owing to its gapped electronic structure, relatively high carrier mobility, and val... As a kind of two-dimensional transition metal dichalcogenide material, tungsten diselenide(WSe_2) has attracted increasing attention, owing to its gapped electronic structure, relatively high carrier mobility, and valley pseudospin, all of which show its valuable nonlinear optical properties. There are few studies on the nonlinear optical properties of WSe_2 and correlation with its electronic structure. In this paper, the effects of spatial self-phase modulation(SSPM) and distortion influence of WSe_2 ethanol suspensions are systematically studied, namely,the nonlinear refractive index and third-order nonlinear optical effect. We obtained the WSe_2 dispersions SSPM distortion formation mechanism, and through it, we calculated the nonlinear refractive index n_2,nonlinear susceptibility χ^(3), and their wavelength dependence under the excitation of 457 nm, 532 nm, and671 nm lasers. Moreover, by use of its strong and broadband nonlinear optical response, all-optical switching of two different laser beams due to spatial cross-phase modulation has been realized experimentally. Our results are useful for future optical devices, such as all-optical switching and all-optical information conversion. 展开更多
关键词 TUNGSTEN DISELENIDE NONLINEAR ALL-OPTICAL SWITCHING
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