背景与目的:胆道恶性肿瘤预后极差,根治性切除是目前唯一可能治愈的手段。相较传统开腹手术,腹腔镜已被证实在安全性和有效性方面具有优势,但在复杂操作中仍受限。机器人辅助腹腔镜因其高清三维视野与灵活操作系统,被认为可弥补腹腔镜...背景与目的:胆道恶性肿瘤预后极差,根治性切除是目前唯一可能治愈的手段。相较传统开腹手术,腹腔镜已被证实在安全性和有效性方面具有优势,但在复杂操作中仍受限。机器人辅助腹腔镜因其高清三维视野与灵活操作系统,被认为可弥补腹腔镜技术不足。然而,目前缺乏平衡基线差异后机器人辅助腹腔镜与普通腹腔镜在胆道恶性肿瘤治疗中的安全性对比研究。本研究旨在通过倾向性评分匹配(PSM),比较两种术式的短期安全性差异。方法:回顾性纳入中国胆道肿瘤协作组数据库中151例接受根治性切除的患者,其中腹腔镜组128例,机器人辅助腹腔镜组23例。为平衡基线差异,首先进行1∶1倾向性评分匹配(PSM),成功匹配腹腔镜19例与机器人19例;随后以机器人组为基准,再行1∶2 PSM,最终获得腹腔镜36例与机器人18例。比较两组主要结局(中转开腹率、转ICU率、术后并发症)及次要结局(手术时间、术中出血量、输血量、术后住院时间、再手术率、再入院率、住院费用等),并采用多因素回归分析探讨中转开腹与术后住院时间的影响因素。结果:PSM后两组基线特征平衡。主要结局方面,腹腔镜组中转开腹率明显高于机器人组(41.7%vs.0,P=0.001),而两组转ICU率、术后并发症总发生率及不同Clavien-Dindo分级并发症发生率差异无统计学意义(均P>0.05)。次要结局方面,腹腔镜组术后住院时间明显长于机器人组(18.5 d vs.8.0 d,P=0.005);手术时间、术中出血量、输血量、再手术率、再入院率及住院费用等差异无统计学意义(均P>0.05)。中转开腹率的Logistic回归分析中虽未发现有统计学意义的影响因素,但中分化肿瘤、术前CA19-9升高、收获淋巴结增多均显示风险升高趋势。多因素线性回归显示,机器人辅助手术是缩短术后住院时间的独立因素(P=0.024),术前总胆红素(P=0.020)、手术时间(P=0.000)、术后并发症(P=0.006)及二次手术(P=0.005)则与住院时间延长相关。结论:机器人辅助腹腔镜胆道肿瘤根治术在短期安全性方面不劣于普通腹腔镜,且可降低中转开腹率、缩短住院时间。其在复杂解剖或高难度病例中可能具有更大优势,为临床提供了一种可行的微创选择,但其推广仍需综合考虑经济成本及医疗资源。展开更多
Background:Dry eye disease(DED)predominantly results from elevated tear film os-molarity,which can not only cause ocular inconvenience but may lead to visual impair-ments,severely compromising patient well-being and e...Background:Dry eye disease(DED)predominantly results from elevated tear film os-molarity,which can not only cause ocular inconvenience but may lead to visual impair-ments,severely compromising patient well-being and exerting substantial economic burdens as well.Astaxanthin(AST),a member of the xanthophylls and recognized for its robust abilities to combat inflammation and oxidation,is a common dietary sup-plement.Nonetheless,the precise molecular pathways through which AST influences DED are still poorly understood.Methods:Therapeutic targets for AST were identified using data from the GeneCards,PharmMapper,and Swiss Target Prediction databases,and STITCH datasets.Similarly,targets for dry eye disease(DED)were delineated leveraging resources such as the Therapeutic Target Database(TTD),DisGeNET,GeneCards,and OMIM databases,and DrugBank datasets.Interactions among shared targets were charted and dis-played using CytoScape 3.9.0.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were conducted to elucidate the functions of pivotal tar-gets within the protein-protein interaction network.Molecular interactions between AST and key targets were confirmed through molecular docking using AutoDock and PyMOL.Molecular dynamics simulations were performed using GROMACS 2022.3.Viability of human corneal epithelial cells(hCEC)was assessed across varying concen-trations of AST.A mouse model of experimental DED was developed using 0.1%ben-zalkonium chloride(BAC),and the animals were administered 100 mg/kg/day of AST orally for 7 days.The efficacy of the treatments was assessed through a series of di-agnostic tests to evaluate the condition of the ocular surface after the interventions.The levels of inflammation and oxidative stress were quantitatively assessed using methods such as reverse transcription-polymerase chain reaction(RT-PCR),Western blot,and immunofluorescence staining.Results:Network pharmacology suggests that AST may alleviate DED by influenc-ing oxidation-reduction signaling pathways and reducing oxidative stress provoked by BAC.In vivo experiments demonstrated an improved overall condition in AST-administered mice in contrast to the control group.Immunofluorescence staining analyses indicated a decrease in Keap1 protein in the corneal tissues of AST-treated mice and a significant increase in Nrf2 and HO-1 protein.In vitro studies demon-strated that AST significantly enhanced cell viability and suppressed reactive oxy-gen species expression under hyperosmotic(HS)conditions,thereby protecting the human corneal epithelium.Conclusion:AST is capable of shielding mice from BAC-induced DED,decelerating the progression of DED,and mitigating oxidative stress damage under HS conditions in hCEC cells.The protective impact of AST on DED may operate through stimulating the Keap1-Nrf2/HO-1 signaling pathway.Our research findings indicate that AST may be a promising treatment for DED,offering new insights into DED treatment.展开更多
This study aims to investigate the effect of different local testicular treatments and validate common prognostic factors on primary testicular lymphoma （PTL） patients. We retrospectively reviewed the clinical recor...This study aims to investigate the effect of different local testicular treatments and validate common prognostic factors on primary testicular lymphoma （PTL） patients. We retrospectively reviewed the clinical records of 32 patients from 1993 to 2017 diagnosed with PTL and included 22 patients for analysis. The Kaplan-Meier method, Log-rank test, and multivariate Cox proportional hazard regression analysis were applied to evaluate progression-free survival （PFS）, overall survival （OS）, and determine prognosis predictors. The median follow-up time was 30 months. Median OS and PFS were 96 months and 49 months, respectively. In univariate analysis, advanced Ann Arbor stage （Ill/IV） （P 〈 0.001）, B symptoms （P 〈 0.001）, and extranodal involvement other than testis （P = 0.001） were significantly associated with shorter OS and PFS. In multivariate analysis, Ann Arbor stage was significantly associated with OS （OR = 11.58, P = 0.049）, whereas B symptom was significantly associated with PFS （OR = 11.79, P = 0.049）. In the 10 patients with the systemic usage of rituximab, bilateral intervention could improve median OS from 16 to 96 months （P = 0.032）. The study provides preliminary evidence on bilateral intervention in testes in the rituximab era and validates common prognostic factors for Chinese PTL patients.展开更多
BACKGROUND This manuscript describes the first known cases of sick sinus syndrome(SSS)associated with the use of anlotinib in non-small cell lung cancer patients,highlighting the need for increased vigilance and cardi...BACKGROUND This manuscript describes the first known cases of sick sinus syndrome(SSS)associated with the use of anlotinib in non-small cell lung cancer patients,highlighting the need for increased vigilance and cardiac monitoring.CASE SUMMARY Two patients with non-small cell lung cancer developed SSS after 15 months and 5 months of anlotinib treatment,respectively,presenting with syncope and palpit-ations.Electrocardiogram confirmed SSS,and different treatment approaches were taken for each patient.One patient received a dual-chamber permanent pacemaker,while the other discontinued the medication and experienced symptom resolution.CONCLUSION Anlotinib can induce SSS,suggesting that cardiac monitoring is crucial during anlotinib treatment.Individualized management strategies are necessary for affected individuals.展开更多
Objective This study aimed to develop a prediction model to assess the risk of sepsis-induced coagulopathy(SIC)in sepsis patients.Methods We conducted a retrospective study of septic patients admitted to the Intensive...Objective This study aimed to develop a prediction model to assess the risk of sepsis-induced coagulopathy(SIC)in sepsis patients.Methods We conducted a retrospective study of septic patients admitted to the Intensive Care Units of Shandong Provincial Hospital(Central Campus and East Campus),and Shenxian People’s Hospital from January 2019 to September 2024.We used Kaplan-Meier analysis to assess survival outcomes.LASSO regression identified predictive variables,and logistic regression was employed to analyze risk factors for pre-SIC.A nomogram prediction model was developed via R software and evaluated via receiver operating characteristic(ROC)curves,calibration curves,and decision curve analysis(DCA).Results Among 309 patients,236 were in the training set,and 73 were in the test set.The pre-SIC group had higher mortality(44.8%vs.21.3%)and disseminated intravascular coagulation(DIC)incidence(56.3%vs.29.1%)than the non-SIC group.LASSO regression identified lactate,coagulation index,creatinine,and SIC scores as predictors of pre-SIC.The nomogram model demonstrated good calibration,with an AUC of 0.766 in the development cohort and 0.776 in the validation cohort.DCA confirmed the model’s clinical utility.Conclusion SIC is associated with increased mortality,with pre-SIC further increasing the risk of death.The nomogram-based prediction model provides a reliable tool for early SIC identification,potentially improving sepsis management and outcomes.展开更多
This paper provide a comprehensive overview of research findings regarding the pathogen responsible for tobacco brown spot,its occurrence regularity,and integrated control strategies.Additionally,this study provide a ...This paper provide a comprehensive overview of research findings regarding the pathogen responsible for tobacco brown spot,its occurrence regularity,and integrated control strategies.Additionally,this study provide a brief analysis of the challenges encountered in the study of tobacco brown spot,which include the study of pathogenesis and virulence,the breeding of disease-resistant varieties,the screening of low-toxicity and high-efficiency agents,the development of biological control methods with more stable efficacy,and the necessity for accurate prediction and forecasting techniques.展开更多
Alcoholic liver disease(ALD)is one of the major global public health problems.Yeast extract(YE),a product prepared from yeast,has been proven to have antioxidant and anti-inflammatory properties.However,the potential ...Alcoholic liver disease(ALD)is one of the major global public health problems.Yeast extract(YE),a product prepared from yeast,has been proven to have antioxidant and anti-inflammatory properties.However,the potential role of YE in the prevention of ALD remains unclear.The present study aimed to investigate the protective effects of YE on ALD and explore the underlying mechanism based on gut microbiota.The result showed that YE supplementation significantly ameliorated chronic alcohol exposure-induced liver injury in mice.In addition,YE counteracted alcohol-induced gut dysbiosis,intestinal barrier dysfunction,lipopolysaccharide(LPS)leakage-induced inflammatory response in the liver.Moreover,microbiota depletion by a broad-spectrum antibiotic was sufficient to block the protective effect of YE on ALD,indicating the contribution of gut dysbiosis modulation to the hepatoprotective role of YE.Furthermore,we demonstrated the causal relationship between gut microbiota and hepatoprotective effects of YE with the fecal microbiota transplantation(FMT)experiment.Compared with the ALD-FMT mice,gut dysbiosis,intestinal barrier dysfunction,LPS/TLR4 signaling pathway activation,and liver inflammatory response were significantly improved in the YE-FMT mice.Together,our findings highlight that dietary YE protects against ALD through gut dysbiosis correction.展开更多
In this work,the{10–12}tensile twins are introduced to improve the drawability of the AZ31 Mg alloy sheet.Concretely,the drawing depth is increased by 32%compared with the as-received sheet at 200℃.This is because{1...In this work,the{10–12}tensile twins are introduced to improve the drawability of the AZ31 Mg alloy sheet.Concretely,the drawing depth is increased by 32%compared with the as-received sheet at 200℃.This is because{10–12}tensile twins promote the occurrences of many deformation mechanisms during warm deep drawing,such as slips,detwinning,dynamic recrystallization(DRX)behaviors,etc.Further,based on the different stress states during deep drawing,these mechanisms and their competition relationships,as well as texture evolutions,are systematically studied.Combined with critical resolved shear stress(CRSS)and microstructure evolution,the global Schmid factor(GSF)obtained by quantizing stress states by stress tensor(σ)can accurately predict the activation trend of deformation mechanisms.It is found that the stress states have a reverse influence on the activation trend of the{10–12}twinning and detwinning.The change of stress states affects the competitive relationships between detwinning and DRX,and then affects the process and degree of DRX.The{10–12}tensile twins and large plane strain promote the activation of prismatic slips,and the larger plane strain also deflected the{10–12}twinning lattice.The{10–12}tensile twins and their induced deformation mechanisms can prominently weaken the basal texture and improve the drawability.展开更多
Indigenous microbial communities were employed after subculture in stirred and column bioleaching experiments involving ion-adsorption type rare earth ore.The microbial eukaryotic communities exhibited dramatically va...Indigenous microbial communities were employed after subculture in stirred and column bioleaching experiments involving ion-adsorption type rare earth ore.The microbial eukaryotic communities exhibited dramatically varying diversity and structure across culture compositions.Compared with Czapek and sucrose medium,the community cultured in a nutrient broth(NB)medium had a high-er diversity,and it was mainly composed of Zygosaccharomyces,Ustilago,Kodamaea,Malassezia,and Aspergillus.These microorgan-isms secrete organic acids,such as citric acid,malic acid,gluconic acid,and itaconic acid,which provide effective coordination electrons through hydroxyl and carboxyl groups.Stirred bioleaching experiments were conducted to investigate the effect of community,inoculum dosage,liquid–solid ratio,and time on the leaching efficiency.Stirred bioleaching resulted in a concentration limitation phenomenon.When the inoculum dosage of the community cultured in NB medium was 70vol%,the liquid–solid ratio was 5.0 mL·g^(-1),and the time was 60 min,the upward trend of rare earths leaching rate has become very small.Specifically,the leaching rates of detectable La,Ce,and Y were approximately 92.49%,92.42%,and 94.39%,respectively.The leaching efficiency and the three influencing factors all con-formed to the Poly5 polynomial function,with variances above 0.99.Column bioleaching experiments were performed at a scale of 1 kg.The self-propelled low-pH environment increased the leaching efficiency,which resulted in a leaching rate of 98.88%for rare earths after 117 h.X-ray diffraction and scanning electron microscopy revealed that the samples mainly comprised quartz,kaolinite,orthoclase,mus-covite,and zeolite,which were predominantly present in the form of lumps,flakes,rods,and small particles.After bioleaching,the wave intensity of quartz,kaolinite,orthoclase,and muscovite increased,and that of zeolite decreased considerably.A diminution in the number of fine particles indicated the dissolution of small quantities of clay minerals.Ultimately,the differentiated bioleaching mechanism of various forms of rare earths was discussed based on experimental phenomena.展开更多
Drought caused by extreme climate change has become more severe and unpredictable,causing imperceptible effects on leaf photosynthesis in foxtail millet.To investigate the damage,we performed light drought(LD)and heav...Drought caused by extreme climate change has become more severe and unpredictable,causing imperceptible effects on leaf photosynthesis in foxtail millet.To investigate the damage,we performed light drought(LD)and heavy drought(HD)treatments at both the elongation(Y)and booting stages to obtain a comprehensive understanding of the morphological,anatomical,physiological,transcriptome,and metabolome levels.Under drought stress,the length and area of leaves decreased,especially during the HD treatment at the booting stage.The number of mesophyll cells and the area of large vascular bundles decreased under LD and HD treatments at the booting stage,with more blurring vascular bundle structure and Kranz anatomy.However,these numbers decreased with no significance under Y-LD and Y-HD treatments at the elongation stage.The net photosynthetic rate,stomatal conductivity,transpiration rate,and intercellular CO_(2)concentration significantly decreased at the booting stage.In addition,the efficiency of electron transfers in photosystem II(PSII)decreased.Conjunction analyses of the transcriptome and metabolome were utilized to uncover the underlying mechanism at the booting stage.The results showed no common differentially enriched pathway in the transcriptome and metabolome under LD treatment.However,32 pathways were enriched in both the transcript and metabolome under HD treatment.Among these,three pathways,including arginine and proline metabolism,tyrosine metabolism,and ubiquinone,along with other terpenoid-quinone biosynthesis pathways,were differentially enriched in both the transcript and metabolome.The accumulation of homogentisate,salidroside,homoprotocatechuate,L-DOPA,tyramine,and L-tyrosine increased under drought stress.Although genes related to PSII and the Calvin cycle were slightly up-regulated under LD conditions,they were down-regulated under HD conditions.The metabolites of ribose-5P,glycerate-3P,D-fructose-1,6P2,and D-fructose-6P were all decreased in both the LD and HD treatments,especially D-fructose-6P,confirming that drought stress harmed the Calvin cycle.The results revealed that regardless of the severity of drought,the photosynthetic function was compromised not only at the morphological and anatomical levels but also in terms of impaired ATP synthase and inhibited photosynthetic CO_(2)assimilation.展开更多
Salt stress is a major constraint to crop productivity and quality.The limited availability of salt-tolerant genes poses significant challenges to breeding programs aimed at enhancing salt tolerance.Sorghum displays a...Salt stress is a major constraint to crop productivity and quality.The limited availability of salt-tolerant genes poses significant challenges to breeding programs aimed at enhancing salt tolerance.Sorghum displays a remarkable ability to withstand saline conditions;therefore,elucidating the genetic underpinnings of this trait is crucial.This study entailed a comprehensive resequencing of 186 sorghum accessions to perform a genome-wide association study(GWAS)focusing on relative root length(RL)and root fresh weight(RFW)under salt stress conditions.We identified eight candidate genes within a co-localized region,among which SbTEF1-a gene encoding a transcription elongation factor protein-was deemed a potential candidate due to its annotation and expression pattern alterations under salt stress.Haplotype analysis,gene cloning,linkage disequilibrium(LD)analysis,and allele effect analysis revealed that PAV284,located in the promoter region of SbTEF1,modulated gene expression under salt stress,which,in turn,influenced sorghum seedlings’salt tolerance.PAV284 holds promise as a genetic marker for selecting salt-tolerant germplasm via marker-assisted breeding,enhancing the development of salt-tolerant sorghum cultivars.展开更多
文摘背景与目的:胆道恶性肿瘤预后极差,根治性切除是目前唯一可能治愈的手段。相较传统开腹手术,腹腔镜已被证实在安全性和有效性方面具有优势,但在复杂操作中仍受限。机器人辅助腹腔镜因其高清三维视野与灵活操作系统,被认为可弥补腹腔镜技术不足。然而,目前缺乏平衡基线差异后机器人辅助腹腔镜与普通腹腔镜在胆道恶性肿瘤治疗中的安全性对比研究。本研究旨在通过倾向性评分匹配(PSM),比较两种术式的短期安全性差异。方法:回顾性纳入中国胆道肿瘤协作组数据库中151例接受根治性切除的患者,其中腹腔镜组128例,机器人辅助腹腔镜组23例。为平衡基线差异,首先进行1∶1倾向性评分匹配(PSM),成功匹配腹腔镜19例与机器人19例;随后以机器人组为基准,再行1∶2 PSM,最终获得腹腔镜36例与机器人18例。比较两组主要结局(中转开腹率、转ICU率、术后并发症)及次要结局(手术时间、术中出血量、输血量、术后住院时间、再手术率、再入院率、住院费用等),并采用多因素回归分析探讨中转开腹与术后住院时间的影响因素。结果:PSM后两组基线特征平衡。主要结局方面,腹腔镜组中转开腹率明显高于机器人组(41.7%vs.0,P=0.001),而两组转ICU率、术后并发症总发生率及不同Clavien-Dindo分级并发症发生率差异无统计学意义(均P>0.05)。次要结局方面,腹腔镜组术后住院时间明显长于机器人组(18.5 d vs.8.0 d,P=0.005);手术时间、术中出血量、输血量、再手术率、再入院率及住院费用等差异无统计学意义(均P>0.05)。中转开腹率的Logistic回归分析中虽未发现有统计学意义的影响因素,但中分化肿瘤、术前CA19-9升高、收获淋巴结增多均显示风险升高趋势。多因素线性回归显示,机器人辅助手术是缩短术后住院时间的独立因素(P=0.024),术前总胆红素(P=0.020)、手术时间(P=0.000)、术后并发症(P=0.006)及二次手术(P=0.005)则与住院时间延长相关。结论:机器人辅助腹腔镜胆道肿瘤根治术在短期安全性方面不劣于普通腹腔镜,且可降低中转开腹率、缩短住院时间。其在复杂解剖或高难度病例中可能具有更大优势,为临床提供了一种可行的微创选择,但其推广仍需综合考虑经济成本及医疗资源。
基金supported by grants from the Beijing Municipal Public Welfare Development and Reform Pilot Project for Medical Research Institutes(PWD&RPP-MRI,JYY2023-6)the R&D Program of Beijing Municipal Education Commission(KZ20231002543).
文摘Background:Dry eye disease(DED)predominantly results from elevated tear film os-molarity,which can not only cause ocular inconvenience but may lead to visual impair-ments,severely compromising patient well-being and exerting substantial economic burdens as well.Astaxanthin(AST),a member of the xanthophylls and recognized for its robust abilities to combat inflammation and oxidation,is a common dietary sup-plement.Nonetheless,the precise molecular pathways through which AST influences DED are still poorly understood.Methods:Therapeutic targets for AST were identified using data from the GeneCards,PharmMapper,and Swiss Target Prediction databases,and STITCH datasets.Similarly,targets for dry eye disease(DED)were delineated leveraging resources such as the Therapeutic Target Database(TTD),DisGeNET,GeneCards,and OMIM databases,and DrugBank datasets.Interactions among shared targets were charted and dis-played using CytoScape 3.9.0.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were conducted to elucidate the functions of pivotal tar-gets within the protein-protein interaction network.Molecular interactions between AST and key targets were confirmed through molecular docking using AutoDock and PyMOL.Molecular dynamics simulations were performed using GROMACS 2022.3.Viability of human corneal epithelial cells(hCEC)was assessed across varying concen-trations of AST.A mouse model of experimental DED was developed using 0.1%ben-zalkonium chloride(BAC),and the animals were administered 100 mg/kg/day of AST orally for 7 days.The efficacy of the treatments was assessed through a series of di-agnostic tests to evaluate the condition of the ocular surface after the interventions.The levels of inflammation and oxidative stress were quantitatively assessed using methods such as reverse transcription-polymerase chain reaction(RT-PCR),Western blot,and immunofluorescence staining.Results:Network pharmacology suggests that AST may alleviate DED by influenc-ing oxidation-reduction signaling pathways and reducing oxidative stress provoked by BAC.In vivo experiments demonstrated an improved overall condition in AST-administered mice in contrast to the control group.Immunofluorescence staining analyses indicated a decrease in Keap1 protein in the corneal tissues of AST-treated mice and a significant increase in Nrf2 and HO-1 protein.In vitro studies demon-strated that AST significantly enhanced cell viability and suppressed reactive oxy-gen species expression under hyperosmotic(HS)conditions,thereby protecting the human corneal epithelium.Conclusion:AST is capable of shielding mice from BAC-induced DED,decelerating the progression of DED,and mitigating oxidative stress damage under HS conditions in hCEC cells.The protective impact of AST on DED may operate through stimulating the Keap1-Nrf2/HO-1 signaling pathway.Our research findings indicate that AST may be a promising treatment for DED,offering new insights into DED treatment.
文摘This study aims to investigate the effect of different local testicular treatments and validate common prognostic factors on primary testicular lymphoma （PTL） patients. We retrospectively reviewed the clinical records of 32 patients from 1993 to 2017 diagnosed with PTL and included 22 patients for analysis. The Kaplan-Meier method, Log-rank test, and multivariate Cox proportional hazard regression analysis were applied to evaluate progression-free survival （PFS）, overall survival （OS）, and determine prognosis predictors. The median follow-up time was 30 months. Median OS and PFS were 96 months and 49 months, respectively. In univariate analysis, advanced Ann Arbor stage （Ill/IV） （P 〈 0.001）, B symptoms （P 〈 0.001）, and extranodal involvement other than testis （P = 0.001） were significantly associated with shorter OS and PFS. In multivariate analysis, Ann Arbor stage was significantly associated with OS （OR = 11.58, P = 0.049）, whereas B symptom was significantly associated with PFS （OR = 11.79, P = 0.049）. In the 10 patients with the systemic usage of rituximab, bilateral intervention could improve median OS from 16 to 96 months （P = 0.032）. The study provides preliminary evidence on bilateral intervention in testes in the rituximab era and validates common prognostic factors for Chinese PTL patients.
文摘BACKGROUND This manuscript describes the first known cases of sick sinus syndrome(SSS)associated with the use of anlotinib in non-small cell lung cancer patients,highlighting the need for increased vigilance and cardiac monitoring.CASE SUMMARY Two patients with non-small cell lung cancer developed SSS after 15 months and 5 months of anlotinib treatment,respectively,presenting with syncope and palpit-ations.Electrocardiogram confirmed SSS,and different treatment approaches were taken for each patient.One patient received a dual-chamber permanent pacemaker,while the other discontinued the medication and experienced symptom resolution.CONCLUSION Anlotinib can induce SSS,suggesting that cardiac monitoring is crucial during anlotinib treatment.Individualized management strategies are necessary for affected individuals.
基金funded by the Shandong Provincial Natural Science Foundation(No.ZR2024MH008)Postdoctoral Innovation Program of Shandong Province(No.SDCX-ZG-202400043)Beijing iGandan Foundation(No.iGandanF-1082022-RGG007).
文摘Objective This study aimed to develop a prediction model to assess the risk of sepsis-induced coagulopathy(SIC)in sepsis patients.Methods We conducted a retrospective study of septic patients admitted to the Intensive Care Units of Shandong Provincial Hospital(Central Campus and East Campus),and Shenxian People’s Hospital from January 2019 to September 2024.We used Kaplan-Meier analysis to assess survival outcomes.LASSO regression identified predictive variables,and logistic regression was employed to analyze risk factors for pre-SIC.A nomogram prediction model was developed via R software and evaluated via receiver operating characteristic(ROC)curves,calibration curves,and decision curve analysis(DCA).Results Among 309 patients,236 were in the training set,and 73 were in the test set.The pre-SIC group had higher mortality(44.8%vs.21.3%)and disseminated intravascular coagulation(DIC)incidence(56.3%vs.29.1%)than the non-SIC group.LASSO regression identified lactate,coagulation index,creatinine,and SIC scores as predictors of pre-SIC.The nomogram model demonstrated good calibration,with an AUC of 0.766 in the development cohort and 0.776 in the validation cohort.DCA confirmed the model’s clinical utility.Conclusion SIC is associated with increased mortality,with pre-SIC further increasing the risk of death.The nomogram-based prediction model provides a reliable tool for early SIC identification,potentially improving sepsis management and outcomes.
文摘This paper provide a comprehensive overview of research findings regarding the pathogen responsible for tobacco brown spot,its occurrence regularity,and integrated control strategies.Additionally,this study provide a brief analysis of the challenges encountered in the study of tobacco brown spot,which include the study of pathogenesis and virulence,the breeding of disease-resistant varieties,the screening of low-toxicity and high-efficiency agents,the development of biological control methods with more stable efficacy,and the necessity for accurate prediction and forecasting techniques.
基金supported by Angel Nutritech Nutrition Fund(AF2021003)。
文摘Alcoholic liver disease(ALD)is one of the major global public health problems.Yeast extract(YE),a product prepared from yeast,has been proven to have antioxidant and anti-inflammatory properties.However,the potential role of YE in the prevention of ALD remains unclear.The present study aimed to investigate the protective effects of YE on ALD and explore the underlying mechanism based on gut microbiota.The result showed that YE supplementation significantly ameliorated chronic alcohol exposure-induced liver injury in mice.In addition,YE counteracted alcohol-induced gut dysbiosis,intestinal barrier dysfunction,lipopolysaccharide(LPS)leakage-induced inflammatory response in the liver.Moreover,microbiota depletion by a broad-spectrum antibiotic was sufficient to block the protective effect of YE on ALD,indicating the contribution of gut dysbiosis modulation to the hepatoprotective role of YE.Furthermore,we demonstrated the causal relationship between gut microbiota and hepatoprotective effects of YE with the fecal microbiota transplantation(FMT)experiment.Compared with the ALD-FMT mice,gut dysbiosis,intestinal barrier dysfunction,LPS/TLR4 signaling pathway activation,and liver inflammatory response were significantly improved in the YE-FMT mice.Together,our findings highlight that dietary YE protects against ALD through gut dysbiosis correction.
基金supported by the National Natural Science Foundations of China[No.52374395,52474419]Natural Science Foundation of Chongqing[CSTB2024NSCQMSX0267]+6 种基金the Natural Science Foundation of Shanxi province[No.20210302123135,20210302123163]the China Postdoctoral Science Foundation[No.2022M710541]the Research Project Supported by Shanxi Scholarship Council of China[No.2022-038]Scientific and Technological Achievements Transformation Guidance Special Project of Shanxi Province[202104021301022,202204021301009]the Ministry of Science and Higher Education of the Russian Federation for financial support under the Megagrant[no.075-15-2022-1133]he National Research Foundation(NRF)grant funded by the Ministry of Science and ICT[2015R1A2A1A01006795]Korea through the Research Institute of Advanced Materials.
文摘In this work,the{10–12}tensile twins are introduced to improve the drawability of the AZ31 Mg alloy sheet.Concretely,the drawing depth is increased by 32%compared with the as-received sheet at 200℃.This is because{10–12}tensile twins promote the occurrences of many deformation mechanisms during warm deep drawing,such as slips,detwinning,dynamic recrystallization(DRX)behaviors,etc.Further,based on the different stress states during deep drawing,these mechanisms and their competition relationships,as well as texture evolutions,are systematically studied.Combined with critical resolved shear stress(CRSS)and microstructure evolution,the global Schmid factor(GSF)obtained by quantizing stress states by stress tensor(σ)can accurately predict the activation trend of deformation mechanisms.It is found that the stress states have a reverse influence on the activation trend of the{10–12}twinning and detwinning.The change of stress states affects the competitive relationships between detwinning and DRX,and then affects the process and degree of DRX.The{10–12}tensile twins and large plane strain promote the activation of prismatic slips,and the larger plane strain also deflected the{10–12}twinning lattice.The{10–12}tensile twins and their induced deformation mechanisms can prominently weaken the basal texture and improve the drawability.
基金supported by the Key Laboratory of Ionic Rare Earth Resources and Environment,Ministry of Natural Resources of the People’s Republic of China(No.2022IRERE201)the Natural Science Foundation of Jiangsu Province,China(No.BK20231074)+4 种基金the National Natural Science Foundation of China(Nos.52404371,52274351,and 52304365)the National Key Research and Development Project of China(No.2021YFC2902202)the Natural Science Foundation of Guangdong Province,China(No.2023A1515011847)the Natural Science Foundation of Jiangxi Province,China(Nos.20232ACB204014 and 20232ACB204016)the Open Fund for Technology Innovation Center for Comprehensive Utilization of Strategic Mineral Resources of Ministry of Natural Resources(No.CCUM-KY-2305).
文摘Indigenous microbial communities were employed after subculture in stirred and column bioleaching experiments involving ion-adsorption type rare earth ore.The microbial eukaryotic communities exhibited dramatically varying diversity and structure across culture compositions.Compared with Czapek and sucrose medium,the community cultured in a nutrient broth(NB)medium had a high-er diversity,and it was mainly composed of Zygosaccharomyces,Ustilago,Kodamaea,Malassezia,and Aspergillus.These microorgan-isms secrete organic acids,such as citric acid,malic acid,gluconic acid,and itaconic acid,which provide effective coordination electrons through hydroxyl and carboxyl groups.Stirred bioleaching experiments were conducted to investigate the effect of community,inoculum dosage,liquid–solid ratio,and time on the leaching efficiency.Stirred bioleaching resulted in a concentration limitation phenomenon.When the inoculum dosage of the community cultured in NB medium was 70vol%,the liquid–solid ratio was 5.0 mL·g^(-1),and the time was 60 min,the upward trend of rare earths leaching rate has become very small.Specifically,the leaching rates of detectable La,Ce,and Y were approximately 92.49%,92.42%,and 94.39%,respectively.The leaching efficiency and the three influencing factors all con-formed to the Poly5 polynomial function,with variances above 0.99.Column bioleaching experiments were performed at a scale of 1 kg.The self-propelled low-pH environment increased the leaching efficiency,which resulted in a leaching rate of 98.88%for rare earths after 117 h.X-ray diffraction and scanning electron microscopy revealed that the samples mainly comprised quartz,kaolinite,orthoclase,mus-covite,and zeolite,which were predominantly present in the form of lumps,flakes,rods,and small particles.After bioleaching,the wave intensity of quartz,kaolinite,orthoclase,and muscovite increased,and that of zeolite decreased considerably.A diminution in the number of fine particles indicated the dissolution of small quantities of clay minerals.Ultimately,the differentiated bioleaching mechanism of various forms of rare earths was discussed based on experimental phenomena.
基金supported by the National Key Research and Development Program of China(2019YFD1002204)the National Natural Science Foundation for Youth of China(31901505)the Shenyang Agricultural University Introduced Talent Research Project,China(20153042)。
文摘Drought caused by extreme climate change has become more severe and unpredictable,causing imperceptible effects on leaf photosynthesis in foxtail millet.To investigate the damage,we performed light drought(LD)and heavy drought(HD)treatments at both the elongation(Y)and booting stages to obtain a comprehensive understanding of the morphological,anatomical,physiological,transcriptome,and metabolome levels.Under drought stress,the length and area of leaves decreased,especially during the HD treatment at the booting stage.The number of mesophyll cells and the area of large vascular bundles decreased under LD and HD treatments at the booting stage,with more blurring vascular bundle structure and Kranz anatomy.However,these numbers decreased with no significance under Y-LD and Y-HD treatments at the elongation stage.The net photosynthetic rate,stomatal conductivity,transpiration rate,and intercellular CO_(2)concentration significantly decreased at the booting stage.In addition,the efficiency of electron transfers in photosystem II(PSII)decreased.Conjunction analyses of the transcriptome and metabolome were utilized to uncover the underlying mechanism at the booting stage.The results showed no common differentially enriched pathway in the transcriptome and metabolome under LD treatment.However,32 pathways were enriched in both the transcript and metabolome under HD treatment.Among these,three pathways,including arginine and proline metabolism,tyrosine metabolism,and ubiquinone,along with other terpenoid-quinone biosynthesis pathways,were differentially enriched in both the transcript and metabolome.The accumulation of homogentisate,salidroside,homoprotocatechuate,L-DOPA,tyramine,and L-tyrosine increased under drought stress.Although genes related to PSII and the Calvin cycle were slightly up-regulated under LD conditions,they were down-regulated under HD conditions.The metabolites of ribose-5P,glycerate-3P,D-fructose-1,6P2,and D-fructose-6P were all decreased in both the LD and HD treatments,especially D-fructose-6P,confirming that drought stress harmed the Calvin cycle.The results revealed that regardless of the severity of drought,the photosynthetic function was compromised not only at the morphological and anatomical levels but also in terms of impaired ATP synthase and inhibited photosynthetic CO_(2)assimilation.
基金supported by the Science and Technology Program in Liaoning Province,China(2022-BS-172)the China Agriculture Research System of MOF and MARA(CARS-06-14.5-A17)the Guiding Funds of the Central Government for the Local Scientific and Technological Development Basic Research Program of Jilin Province,China(202002068JC).
文摘Salt stress is a major constraint to crop productivity and quality.The limited availability of salt-tolerant genes poses significant challenges to breeding programs aimed at enhancing salt tolerance.Sorghum displays a remarkable ability to withstand saline conditions;therefore,elucidating the genetic underpinnings of this trait is crucial.This study entailed a comprehensive resequencing of 186 sorghum accessions to perform a genome-wide association study(GWAS)focusing on relative root length(RL)and root fresh weight(RFW)under salt stress conditions.We identified eight candidate genes within a co-localized region,among which SbTEF1-a gene encoding a transcription elongation factor protein-was deemed a potential candidate due to its annotation and expression pattern alterations under salt stress.Haplotype analysis,gene cloning,linkage disequilibrium(LD)analysis,and allele effect analysis revealed that PAV284,located in the promoter region of SbTEF1,modulated gene expression under salt stress,which,in turn,influenced sorghum seedlings’salt tolerance.PAV284 holds promise as a genetic marker for selecting salt-tolerant germplasm via marker-assisted breeding,enhancing the development of salt-tolerant sorghum cultivars.
