目的:探讨芪地固肾方治疗膜性肾病(MN)大鼠的效果及其可能的机制。方法:将40只SD雄性大鼠随机分为模型组(等剂量生理盐水尾静脉注射)、雷公藤多甙片组(10 mg/kg雷公藤多甙片)、芪地固肾方低剂量组(15.425 g/kg芪地固肾方)、芪地固肾方...目的:探讨芪地固肾方治疗膜性肾病(MN)大鼠的效果及其可能的机制。方法:将40只SD雄性大鼠随机分为模型组(等剂量生理盐水尾静脉注射)、雷公藤多甙片组(10 mg/kg雷公藤多甙片)、芪地固肾方低剂量组(15.425 g/kg芪地固肾方)、芪地固肾方高剂量组(61.7 g/kg芪地固肾方)四组,另取10只未造模大鼠作为空白组(等剂量生理盐水尾静脉注射),连续干预28天后,检测24小时尿蛋白定量(U-TP)、血清总蛋白(TP)、白蛋白(ALB)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、血肌酐(Scr)、尿素氮(BUN)的变化;HE染色、透射电镜观察大鼠肾组织病理学改变;RT-PCR检测肾组织中nephrin蛋白、podocin m RNA的表达。结果:与空白组比较,模型组24 hU-TP、足细胞nephrin和podocin m RNA显著升高,血清TP和ALB显著降低(P<0.01);与模型组比较,各给药组24 hU-TP降低,血清TP和ALB显著升高(P<0.05);肾组织免疫复合物沉积减少,肾小球基底膜增厚减轻;足细胞nephrin和podocin m RNA表达升高(P<0.05)。结论:芪地固肾方能够降低MN模型大鼠的尿蛋白水平,减轻肾脏病理损伤,上调肾组织中足细胞裂孔隔膜蛋白nephrin和podocin m RNA的表达,延缓膜性肾病的进展。展开更多
Radix bupleuri is the dried root of Umbelliferae plants Bupleurem chinense DC.or Bupleurem scorzonerifolium Willd.,which are widely distributed in Northeast China,North China,Northwest China,East China,Hubei,Sichuan a...Radix bupleuri is the dried root of Umbelliferae plants Bupleurem chinense DC.or Bupleurem scorzonerifolium Willd.,which are widely distributed in Northeast China,North China,Northwest China,East China,Hubei,Sichuan and other places.The plant roots are excavated in spring and autumn and dried with removing stems and leaves and sediment.Radix bupleuri,one of the oldest and most commonly used Chinese herbal medicine,has a long history of application,with high medicinal value.Modern pharmacological studies have shown that Radix bupleuri has pharmacological effects such as anti-cancer,anti-depression,anti-inflammation,heart protection,liver protection,and kidney protection,and mainly plays an role in regulating apoptosis signaling pathway,inflammation signaling pathway,neuroendocrine system,oxidative stress signaling pathway,and fibrosis signaling pathway.This paper reviews the modern pharmacological effects of Radix bupleuri in recent years,in order to provide reference for better research,development and rational utilization of Bupleurum resources.展开更多
文摘目的:探讨芪地固肾方治疗膜性肾病(MN)大鼠的效果及其可能的机制。方法:将40只SD雄性大鼠随机分为模型组(等剂量生理盐水尾静脉注射)、雷公藤多甙片组(10 mg/kg雷公藤多甙片)、芪地固肾方低剂量组(15.425 g/kg芪地固肾方)、芪地固肾方高剂量组(61.7 g/kg芪地固肾方)四组,另取10只未造模大鼠作为空白组(等剂量生理盐水尾静脉注射),连续干预28天后,检测24小时尿蛋白定量(U-TP)、血清总蛋白(TP)、白蛋白(ALB)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、血肌酐(Scr)、尿素氮(BUN)的变化;HE染色、透射电镜观察大鼠肾组织病理学改变;RT-PCR检测肾组织中nephrin蛋白、podocin m RNA的表达。结果:与空白组比较,模型组24 hU-TP、足细胞nephrin和podocin m RNA显著升高,血清TP和ALB显著降低(P<0.01);与模型组比较,各给药组24 hU-TP降低,血清TP和ALB显著升高(P<0.05);肾组织免疫复合物沉积减少,肾小球基底膜增厚减轻;足细胞nephrin和podocin m RNA表达升高(P<0.05)。结论:芪地固肾方能够降低MN模型大鼠的尿蛋白水平,减轻肾脏病理损伤,上调肾组织中足细胞裂孔隔膜蛋白nephrin和podocin m RNA的表达,延缓膜性肾病的进展。
基金Construction Project of Inheritance Studio of Lei Genping,A Famous TCM Doctor of Shaanxi Province(Shaanxi TCM Letter[2019]No.82,Studio No.2019012)First-class Discipline Innovation Team Project of Shaanxi University of Traditional Chinese Medicine(No.2019-YL04)+1 种基金Xianyang Science and Technology Innovation Team Project"Clinical Innovation Team of Traditional Chinese Medicine in the Treatment of Chronic Nephritis"({2019}No.46)Shaanxi Education,Science,Culture,Health and Sports System Staff Lei Genping(Model Worker)Innovation Studio({2020}No.41)。
文摘Radix bupleuri is the dried root of Umbelliferae plants Bupleurem chinense DC.or Bupleurem scorzonerifolium Willd.,which are widely distributed in Northeast China,North China,Northwest China,East China,Hubei,Sichuan and other places.The plant roots are excavated in spring and autumn and dried with removing stems and leaves and sediment.Radix bupleuri,one of the oldest and most commonly used Chinese herbal medicine,has a long history of application,with high medicinal value.Modern pharmacological studies have shown that Radix bupleuri has pharmacological effects such as anti-cancer,anti-depression,anti-inflammation,heart protection,liver protection,and kidney protection,and mainly plays an role in regulating apoptosis signaling pathway,inflammation signaling pathway,neuroendocrine system,oxidative stress signaling pathway,and fibrosis signaling pathway.This paper reviews the modern pharmacological effects of Radix bupleuri in recent years,in order to provide reference for better research,development and rational utilization of Bupleurum resources.
