目的比较腹腔镜辅助胰十二指肠切除术(LAPD)与开腹胰十二指肠切除术(OPD)的近期疗效,探讨LAPD的手术安全性及可行性。方法回顾性分析2015年10月至2017年12月沧州市中心医院普外科施行的102例LAPD和179例OPD患者的临床资料,将两组各项临...目的比较腹腔镜辅助胰十二指肠切除术(LAPD)与开腹胰十二指肠切除术(OPD)的近期疗效,探讨LAPD的手术安全性及可行性。方法回顾性分析2015年10月至2017年12月沧州市中心医院普外科施行的102例LAPD和179例OPD患者的临床资料,将两组各项临床数据进行对比分析。结果 LAPD组手术时间显著长于OPD组[(381±67)min vs (245±43)min],但LAPD组术中出血量更少[(186±102)mL vs(322±75)mL],手术切口更小[(8.1±3.9)cm vs (18.9±4.3)cm],术后进流食时间、术后ICU入住时间及术后住院时间均更短,两组比较差异均有统计学意义(P<0.05)。术中淋巴结清扫数目、R0切除率、术后并发症发生率及术后6个月无瘤生存率的差异均无统计学意义(P>0.05)。结论 LAPD具有与传统OPD手术相似的安全性和肿瘤根治效果,而且具有创伤小、恢复快等优势。但LAPD手术时间更长,对术者经验要求更高。展开更多
Carbon black(CB)is a vital constituent of airborne pollutants,comprising diesel exhaust and fine particulate matter(PM_(2.5)),aswell as a prevalent manufacturingmaterial.CBwas known to cause pulmonary dysfunction and ...Carbon black(CB)is a vital constituent of airborne pollutants,comprising diesel exhaust and fine particulate matter(PM_(2.5)),aswell as a prevalent manufacturingmaterial.CBwas known to cause pulmonary dysfunction and fibrosis.However,the detailedmolecular mechanisms underlying fibrosis development are poorly understood.In this study,18 C57BL/6mice were randomized into two groups and exposed to CB and filtered air(FA)for 28 days,with 6 hr/day and 7 days perweek exposure regimen,respectively.The human normal bronchial epithelial cell line(BEAS-2B)was subjected to CB treatment for 24 h in vitro,with CB concentrations in 0,50,100,and 200μg/mL.Our study indicated that exposure to CB resulted in a reduction in lung function and the development of pulmonary fibrosis in mice.Furthermore,our results showed cytoskeleton rearrangement and epithelial-mesenchymal transition(EMT)phenotype in BEAS-2B cells were happened,after CB exposure.Subsequent studies revealed that elevated expression of THBS2 after CB primarily contributed to the development of pulmonary fibrosis.The research findings from both in vivo and in vitro studies provided evidence that piR-713551 was involved in CB exposure-induced EMT by targeting the THBS2 gene and activating theβ-catenin pathway.Mechanically,piR-713551/PIWIL4 complex activated the THBS2 transcription by recruitment of histone demethyltransferase KDM4A to reduce H3K9me3 modification at the THBS2 gene promoter.Conclusively,our research showed that CB exposure could activate EMT and lead pulmonary fibrosis which was modulated by piR-713551/PIWIL4 targeting THBS2.展开更多
利用无线通讯网络中的微波链路来监测降雨和水汽等是大气环境监测的新技术之一。这个技术可以测量近地面的降雨强度和水汽密度等气象参数,具有时空分辨率高、成本低等优势。利用瑞典爱立信公司(Ericsson)提供的位于哥德堡地区E频段的微...利用无线通讯网络中的微波链路来监测降雨和水汽等是大气环境监测的新技术之一。这个技术可以测量近地面的降雨强度和水汽密度等气象参数,具有时空分辨率高、成本低等优势。利用瑞典爱立信公司(Ericsson)提供的位于哥德堡地区E频段的微波通讯链路资料、位于链路一端的气象站1资料和由瑞典气象水文研究所(SMHI)气象网站提供的气象站2资料,对2017年06月13日至2017年07月13日近1个月的水汽密度进行反演计算和分析。结果表明:同一区域的不同地点处的气象要素有一定的差异性,同一区域的温度会有一定的浮动(0~4℃),两者之间的相关性为0. 87;微波通讯链路反演的水汽密度结果与研究区域的地面气象站1和气象站2测量结果有很好的一致性,两者之间的相关性分别为0. 89和0. 97,均方根误分别差为0. 75 g m3和0. 79 g m3;利用微波链路,与现有的湿度监测方法相比,可以为现有的天气监测网络提供额外的丰富的数据源。展开更多
文摘目的比较腹腔镜辅助胰十二指肠切除术(LAPD)与开腹胰十二指肠切除术(OPD)的近期疗效,探讨LAPD的手术安全性及可行性。方法回顾性分析2015年10月至2017年12月沧州市中心医院普外科施行的102例LAPD和179例OPD患者的临床资料,将两组各项临床数据进行对比分析。结果 LAPD组手术时间显著长于OPD组[(381±67)min vs (245±43)min],但LAPD组术中出血量更少[(186±102)mL vs(322±75)mL],手术切口更小[(8.1±3.9)cm vs (18.9±4.3)cm],术后进流食时间、术后ICU入住时间及术后住院时间均更短,两组比较差异均有统计学意义(P<0.05)。术中淋巴结清扫数目、R0切除率、术后并发症发生率及术后6个月无瘤生存率的差异均无统计学意义(P>0.05)。结论 LAPD具有与传统OPD手术相似的安全性和肿瘤根治效果,而且具有创伤小、恢复快等优势。但LAPD手术时间更长,对术者经验要求更高。
基金supported by the National Natural Science Foundation of China(Nos.81973074,92043202,82204075,and 82204074).
文摘Carbon black(CB)is a vital constituent of airborne pollutants,comprising diesel exhaust and fine particulate matter(PM_(2.5)),aswell as a prevalent manufacturingmaterial.CBwas known to cause pulmonary dysfunction and fibrosis.However,the detailedmolecular mechanisms underlying fibrosis development are poorly understood.In this study,18 C57BL/6mice were randomized into two groups and exposed to CB and filtered air(FA)for 28 days,with 6 hr/day and 7 days perweek exposure regimen,respectively.The human normal bronchial epithelial cell line(BEAS-2B)was subjected to CB treatment for 24 h in vitro,with CB concentrations in 0,50,100,and 200μg/mL.Our study indicated that exposure to CB resulted in a reduction in lung function and the development of pulmonary fibrosis in mice.Furthermore,our results showed cytoskeleton rearrangement and epithelial-mesenchymal transition(EMT)phenotype in BEAS-2B cells were happened,after CB exposure.Subsequent studies revealed that elevated expression of THBS2 after CB primarily contributed to the development of pulmonary fibrosis.The research findings from both in vivo and in vitro studies provided evidence that piR-713551 was involved in CB exposure-induced EMT by targeting the THBS2 gene and activating theβ-catenin pathway.Mechanically,piR-713551/PIWIL4 complex activated the THBS2 transcription by recruitment of histone demethyltransferase KDM4A to reduce H3K9me3 modification at the THBS2 gene promoter.Conclusively,our research showed that CB exposure could activate EMT and lead pulmonary fibrosis which was modulated by piR-713551/PIWIL4 targeting THBS2.
基金This work was supported by the National Key R&D Program of China(2021YFE0200300,2021YFC1712903)the Technical System of Chinese Medicine Prevention and Treatment of Influenza of the China Academy of Chinese Medical Sciences(No.ZZ13-035-09)the 2020 Annual Graduate Students Innovation Fund,School of Integrative Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin,China(ZXYCXLX202021).
文摘利用无线通讯网络中的微波链路来监测降雨和水汽等是大气环境监测的新技术之一。这个技术可以测量近地面的降雨强度和水汽密度等气象参数,具有时空分辨率高、成本低等优势。利用瑞典爱立信公司(Ericsson)提供的位于哥德堡地区E频段的微波通讯链路资料、位于链路一端的气象站1资料和由瑞典气象水文研究所(SMHI)气象网站提供的气象站2资料,对2017年06月13日至2017年07月13日近1个月的水汽密度进行反演计算和分析。结果表明:同一区域的不同地点处的气象要素有一定的差异性,同一区域的温度会有一定的浮动(0~4℃),两者之间的相关性为0. 87;微波通讯链路反演的水汽密度结果与研究区域的地面气象站1和气象站2测量结果有很好的一致性,两者之间的相关性分别为0. 89和0. 97,均方根误分别差为0. 75 g m3和0. 79 g m3;利用微波链路,与现有的湿度监测方法相比,可以为现有的天气监测网络提供额外的丰富的数据源。
