Objective： Detecting the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudoge...Objective： Detecting the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify ifhTRF1 mutation is one of the factors of the activation of telomerase. Methods： hTRFlcDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of h TRFlmRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-I, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification （TRAP）-ELISA protocol. PCR and sequencing were used to detect mutation of each exon ofhTRF1 in 10 cell line cells. Results： hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes ofhTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRFI-13, ψhTRFI-18, ψhTRF1-21 and ψhTRFI-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells （0.0338, 0.0108-0.0749） than in normal mononuclear cells （0.0493, 0.0369-0.128） （P=0.004）. But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part ofintron 1, 2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion： hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.展开更多
This study deals with complexity, frequency spectrum and velocity model of the crust-mantle transitional zone in different tectonic units along the northeast margin of Qinghai-Xizang plateau, based on PmP waveform dat...This study deals with complexity, frequency spectrum and velocity model of the crust-mantle transitional zone in different tectonic units along the northeast margin of Qinghai-Xizang plateau, based on PmP waveform data from two deep seismic sounding profiles passing through the area. It reveals that Moho has stable tectonic features in Ordos and Lingzhong basins, where crust and mantle are coupled as first-order discontinuity. Moho shows obvious signs of activity in Haiyuan seismic region and in the contact zone between Bayanhar block and Qaidam block. Crust and mantle in these two areas are coupled as complicated crust-mantle transitional zone consisting of multiple laminae with alternate high and low velocities, totaling 20 km in thickness. The difference between Moho of different tectonic units reflects heterogeneity of the coupled crust-mantle zone; the difference between fine structures of Haiyuan seismic region and Maqin fault zone reflects different deep material composition of the two continent-continent collision zones and the interaction between blocks.展开更多
The Cauchy problem for the semilinear wave equation has been studied and results show that the problem is locally well-posed in Hs ( Rn ) for s > max [ 0, n/2 - 1]. We extend the results by Lindblad in R3 to R2 and...The Cauchy problem for the semilinear wave equation has been studied and results show that the problem is locally well-posed in Hs ( Rn ) for s > max [ 0, n/2 - 1]. We extend the results by Lindblad in R3 to R2 and R1. The methods used in this paper are different from those of Lindblad and also the methods are more simple.展开更多
Editorial: AIDS is the archenemy of mankind, it also threatened Chinese people seriously. Although anti-retroviral drugs are effective, yet too expensive, the majority of 600 000 Chinese patients can't afford to p...Editorial: AIDS is the archenemy of mankind, it also threatened Chinese people seriously. Although anti-retroviral drugs are effective, yet too expensive, the majority of 600 000 Chinese patients can't afford to purchase and use them. Decades ago, some scientists were trying to use Chinese Medicine in treating AIDS, experienc e gained but problems also exposed concerning strategy of research, methodol ogy, racial difference, Chinese drugs screening, integrative Chinese and western medicine, HIV and immunity, etc., sustained and integrative effort would bring safe, effective, and affordable drugs for the fatal pandemic all over the world ……展开更多
文摘Objective： Detecting the expression and mutation of human telomeric repeat binding factor （hTRF1） in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify ifhTRF1 mutation is one of the factors of the activation of telomerase. Methods： hTRFlcDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of h TRFlmRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-I, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification （TRAP）-ELISA protocol. PCR and sequencing were used to detect mutation of each exon ofhTRF1 in 10 cell line cells. Results： hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes ofhTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRFI-13, ψhTRFI-18, ψhTRF1-21 and ψhTRFI-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells （0.0338, 0.0108-0.0749） than in normal mononuclear cells （0.0493, 0.0369-0.128） （P=0.004）. But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part ofintron 1, 2 and 8 ofhTRF1. Their infection on gene function is unknown and needs further studies. Conclusion： hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.
基金Chinese Joint Seismological Science Foundation (102025, 104027).Contribution No. RCEG200210, Geophysical Exploration Center, China Earthquake Administration.
文摘This study deals with complexity, frequency spectrum and velocity model of the crust-mantle transitional zone in different tectonic units along the northeast margin of Qinghai-Xizang plateau, based on PmP waveform data from two deep seismic sounding profiles passing through the area. It reveals that Moho has stable tectonic features in Ordos and Lingzhong basins, where crust and mantle are coupled as first-order discontinuity. Moho shows obvious signs of activity in Haiyuan seismic region and in the contact zone between Bayanhar block and Qaidam block. Crust and mantle in these two areas are coupled as complicated crust-mantle transitional zone consisting of multiple laminae with alternate high and low velocities, totaling 20 km in thickness. The difference between Moho of different tectonic units reflects heterogeneity of the coupled crust-mantle zone; the difference between fine structures of Haiyuan seismic region and Maqin fault zone reflects different deep material composition of the two continent-continent collision zones and the interaction between blocks.
文摘The Cauchy problem for the semilinear wave equation has been studied and results show that the problem is locally well-posed in Hs ( Rn ) for s > max [ 0, n/2 - 1]. We extend the results by Lindblad in R3 to R2 and R1. The methods used in this paper are different from those of Lindblad and also the methods are more simple.
文摘Editorial: AIDS is the archenemy of mankind, it also threatened Chinese people seriously. Although anti-retroviral drugs are effective, yet too expensive, the majority of 600 000 Chinese patients can't afford to purchase and use them. Decades ago, some scientists were trying to use Chinese Medicine in treating AIDS, experienc e gained but problems also exposed concerning strategy of research, methodol ogy, racial difference, Chinese drugs screening, integrative Chinese and western medicine, HIV and immunity, etc., sustained and integrative effort would bring safe, effective, and affordable drugs for the fatal pandemic all over the world ……
