Amphiphilic polymers with self-assembling abilities and stimuliresponsive functionalities have drawn significant interest as nanotransport systems for biomedical applications.In this study,we have designed and develop...Amphiphilic polymers with self-assembling abilities and stimuliresponsive functionalities have drawn significant interest as nanotransport systems for biomedical applications.In this study,we have designed and developed dualstimuli-responsive multi-amphiphilic polymeric architectures using easily available biocompatible starting materials.We copolymerized poly(ethylene glycol)[bis-(carboxymethyl)ether]diethylester(PEG-diester)and 3,3′-((2-azidopropane-1,3-diyl)bis(oxy))bis(propane-1,2-diol)(azido-triglycerol)using a biocatalyst,and the copolymer so obtained was grafted with azobenzene and polyglycerol dendron moieties to generate multi-amphiphilic polymeric architectures.The self-assembly and cargo encapsulation behaviors of the synthesized polymers were studied by encapsulating Nile red,a model hydrophobic probe.The controlled release of encapsulated Nile red was investigated by irradiation with UV light or exposure to lipase.The studied nanocarrier exhibited a slow release of Nile red,up to 72%in 10 days in the presence of lipase;however,only an insignificant release was observed in the absence of enzyme.Though the light induced release was found to proceed to a lesser extent,it was faster compared to lipase mediated release.Experimental data established the excellent capabilities of these systems as drug delivery nanocarriers by being non-cytotoxic up to a concentration of 500μg/mL for 72 h.The cellular uptake study of the Nile red encapsulated polymers by confocal laser scanning microscopy suggested that such polymeric architectures may find potential applications as stimuli-responsive nanocarriers.展开更多
基金Financial support from the Institute of Eminence(IoE/2024-25/12/FRP)University of Delhi,is gratefully acknowledged.
文摘Amphiphilic polymers with self-assembling abilities and stimuliresponsive functionalities have drawn significant interest as nanotransport systems for biomedical applications.In this study,we have designed and developed dualstimuli-responsive multi-amphiphilic polymeric architectures using easily available biocompatible starting materials.We copolymerized poly(ethylene glycol)[bis-(carboxymethyl)ether]diethylester(PEG-diester)and 3,3′-((2-azidopropane-1,3-diyl)bis(oxy))bis(propane-1,2-diol)(azido-triglycerol)using a biocatalyst,and the copolymer so obtained was grafted with azobenzene and polyglycerol dendron moieties to generate multi-amphiphilic polymeric architectures.The self-assembly and cargo encapsulation behaviors of the synthesized polymers were studied by encapsulating Nile red,a model hydrophobic probe.The controlled release of encapsulated Nile red was investigated by irradiation with UV light or exposure to lipase.The studied nanocarrier exhibited a slow release of Nile red,up to 72%in 10 days in the presence of lipase;however,only an insignificant release was observed in the absence of enzyme.Though the light induced release was found to proceed to a lesser extent,it was faster compared to lipase mediated release.Experimental data established the excellent capabilities of these systems as drug delivery nanocarriers by being non-cytotoxic up to a concentration of 500μg/mL for 72 h.The cellular uptake study of the Nile red encapsulated polymers by confocal laser scanning microscopy suggested that such polymeric architectures may find potential applications as stimuli-responsive nanocarriers.
