Age-related macular degeneration(AMD)causes blindness due to loss of retinal pigment epithelium(RPE)and photoreceptors(PRs),which comprise the two outermost layers of the retina.Given the small size of the macula and ...Age-related macular degeneration(AMD)causes blindness due to loss of retinal pigment epithelium(RPE)and photoreceptors(PRs),which comprise the two outermost layers of the retina.Given the small size of the macula and the importance of direct contact between RPE and PRs,the use of scaffolds for targeted reconstruction of the outer retina in later stage AMD and other macular dystrophies is particularly attractive.We developed microfabricated,honeycomb-patterned,biodegradable poly(glycerol sebacate)(PGS)scaffolds to deliver organized,adjacent layers of RPE and PRs to the subretinal space.Furthermore,an optimized process was developed to photocure PGS,shortening scaffold production time from days to minutes.The resulting scaffolds robustly supported the seeding of human pluripotent stem cell-derived RPE and PRs,either separately or as a dual celllayered construct.These advanced,economical,and versatile scaffolds can accelerate retinal cell transplantation efforts and benefit patients with AMD and other retinal degenerative diseases.展开更多
基金the Department of Defense through the Vision Research Program under award no.W81XWH-20-1-0655Opinions,interpretations,conclusions,and recommendations are those of the authors and are not necessarily endorsed by the Department of Defense.This work was also supported by Fighting Blindness Canada including philanthropic support from Donna Green and Goldie Feldman,Research to Prevent Blindness,RRF Emmett A.Humble Distinguished Directorship,McPherson Eye Research Institute Sandra Lemke Trout Chair in Eye Research(to D.M.G)+3 种基金NIH grant P51OD011106 to the Wisconsin National Primate Research Center,and NIH grant U54HD090256 to the Waisman Intellectual and Developmental Disabilities Research Center.A.L.L.was supported by the UW-Madison School of Veterinary Medicine Dual DVM/PhD Program,NIH/NEI U24 EY029890Kirschstein NRSA Predoctoral Fellowship NIH/NEI F30 EY031230the Waisman Center from the National Institute of Child Health and Human Development(P50HD105353).
文摘Age-related macular degeneration(AMD)causes blindness due to loss of retinal pigment epithelium(RPE)and photoreceptors(PRs),which comprise the two outermost layers of the retina.Given the small size of the macula and the importance of direct contact between RPE and PRs,the use of scaffolds for targeted reconstruction of the outer retina in later stage AMD and other macular dystrophies is particularly attractive.We developed microfabricated,honeycomb-patterned,biodegradable poly(glycerol sebacate)(PGS)scaffolds to deliver organized,adjacent layers of RPE and PRs to the subretinal space.Furthermore,an optimized process was developed to photocure PGS,shortening scaffold production time from days to minutes.The resulting scaffolds robustly supported the seeding of human pluripotent stem cell-derived RPE and PRs,either separately or as a dual celllayered construct.These advanced,economical,and versatile scaffolds can accelerate retinal cell transplantation efforts and benefit patients with AMD and other retinal degenerative diseases.
