Objective:To evaluate the supplementation effects of vitamin E,vitamin C,superoxide dismutase(SOD),and catalase to diluents on bull cryopreserved epididymal sperm.Methods:Sperm were retrieved from 20 bull testes and w...Objective:To evaluate the supplementation effects of vitamin E,vitamin C,superoxide dismutase(SOD),and catalase to diluents on bull cryopreserved epididymal sperm.Methods:Sperm were retrieved from 20 bull testes and were then supplemented with 0.1 mM vitamin E,5.0 mM vitamin C,100.0 IU/mL SOD,and 100.0μg/mL catalase alone,or in a combination.The control treatment contained no addition.After supplementation,samples were frozen and stored in liquid nitrogen.The sperm parameters including motility,progressive motility,viability,acrosome integrity,plasma membrane integrity,kinematics and DNA damage were evaluated following the thawing process.Results:Vitamin E alone significantly increased the parameters of acrosome and membrane integrity compared to the control treatment(P<0.05).While compared to the control treatment,vitamin C had no improvement effect on sperm characteristics except for membrane integrity.Treatment of vitamin E+vitamin C had a significant improvement in total motility,progressive motility,viability,membrane and acrosome integrity compared to the control and other treatments(P<0.05).Compared to the control treatment,addition of SOD or catalase alone significantly improved the percentages of total motility,progressive motility,viability,membrane and acrosome integrity(P<0.05).Furthermore,SOD+catalase significantly increased total motility,progressive motility,viability,acrosome and membrane integrity characteristics compared to the catalase treatment(P<0.05).Vitamin E alone,vitamin E+vitamin C,and SOD in diluents decreased DNA damages and thereby improved the rate of intact sperm heads.Conclusions:Addition of 100.0 IU/mL SOD alone and 0.1 mM vitamin E+5.0 mM vitamin C,and also 5.0 mM vitamin C+100μg/mL catalase in a combination improves the quality of cryopreserved bull epididymal sperm and could be used for cryopreservation.展开更多
The human placenta is a transient yet crucial organ that plays a key role in sustaining the relationship between the maternal and fetal organisms.Despite its historical classification as"biowaste,"placental ...The human placenta is a transient yet crucial organ that plays a key role in sustaining the relationship between the maternal and fetal organisms.Despite its historical classification as"biowaste,"placental tissues have garnered increasing attention since the early 1900s for their significant medical potential,particularly in wound repair and surgical application.As ethical considerations regarding human placental derivatives have largely been assuaged in many countries,they have gained significant attention due to their versatile applications in various biomedical fields,such as biomedical engineering,regenerative medicine,and pharmacology.Moreover,there is a substantial trend toward various animal product substitutions in laboratory research with human placental derivatives,reflecting a broader commitment to advancing ethical and sustainable research method-ologies.This review provides a comprehensive examination of the current applications of human placental de-rivatives,explores the mechanisms behind their therapeutic effects,and outlines the future potential and directions of this rapidly advancing field.展开更多
文摘Objective:To evaluate the supplementation effects of vitamin E,vitamin C,superoxide dismutase(SOD),and catalase to diluents on bull cryopreserved epididymal sperm.Methods:Sperm were retrieved from 20 bull testes and were then supplemented with 0.1 mM vitamin E,5.0 mM vitamin C,100.0 IU/mL SOD,and 100.0μg/mL catalase alone,or in a combination.The control treatment contained no addition.After supplementation,samples were frozen and stored in liquid nitrogen.The sperm parameters including motility,progressive motility,viability,acrosome integrity,plasma membrane integrity,kinematics and DNA damage were evaluated following the thawing process.Results:Vitamin E alone significantly increased the parameters of acrosome and membrane integrity compared to the control treatment(P<0.05).While compared to the control treatment,vitamin C had no improvement effect on sperm characteristics except for membrane integrity.Treatment of vitamin E+vitamin C had a significant improvement in total motility,progressive motility,viability,membrane and acrosome integrity compared to the control and other treatments(P<0.05).Compared to the control treatment,addition of SOD or catalase alone significantly improved the percentages of total motility,progressive motility,viability,membrane and acrosome integrity(P<0.05).Furthermore,SOD+catalase significantly increased total motility,progressive motility,viability,acrosome and membrane integrity characteristics compared to the catalase treatment(P<0.05).Vitamin E alone,vitamin E+vitamin C,and SOD in diluents decreased DNA damages and thereby improved the rate of intact sperm heads.Conclusions:Addition of 100.0 IU/mL SOD alone and 0.1 mM vitamin E+5.0 mM vitamin C,and also 5.0 mM vitamin C+100μg/mL catalase in a combination improves the quality of cryopreserved bull epididymal sperm and could be used for cryopreservation.
基金supported by research grants from the Belgian Foundation against Cancer(grant OST-2022/2169 awarded to C.A.A.)the Fonds National de la Recherche Scientifique de Belgique(FNRS-PDR convention grant number T.0004.20 awarded to C.A.A.(Ph.D.scholarship awarded to H.V.)+5 种基金FNRS-CR convention grant number J.0057.22 awarded to F.D.,and FC 46825 fellowship awarded to S.M.)the UCLouvain’s Fonds spéciaux de recherche(IREC FSR22 grant awarded to C.A.A.(Ph.D.scholarship awarded to M.J.S.)IRECFSR23MOVEAND fellowship awarded to S.N.)Fondation Saint-Luc(Fetus for Life awarded to F.D.)the French Community of Belgium(ARC Grant 24/29-137 awarded to F.D.,C.W,and C.A.A.)the China Scholarship Council(Ph.D.scholarship awarded to Y.W.,F.S.,Y.L,J.Y.).
文摘The human placenta is a transient yet crucial organ that plays a key role in sustaining the relationship between the maternal and fetal organisms.Despite its historical classification as"biowaste,"placental tissues have garnered increasing attention since the early 1900s for their significant medical potential,particularly in wound repair and surgical application.As ethical considerations regarding human placental derivatives have largely been assuaged in many countries,they have gained significant attention due to their versatile applications in various biomedical fields,such as biomedical engineering,regenerative medicine,and pharmacology.Moreover,there is a substantial trend toward various animal product substitutions in laboratory research with human placental derivatives,reflecting a broader commitment to advancing ethical and sustainable research method-ologies.This review provides a comprehensive examination of the current applications of human placental de-rivatives,explores the mechanisms behind their therapeutic effects,and outlines the future potential and directions of this rapidly advancing field.
