The incidence of heart failure with preserved ejection fraction(HFpEF)increases with the ageing of populations.This study aimed to explore ageing-associated gene signatures in HFpEF to develop new diagnostic biomarker...The incidence of heart failure with preserved ejection fraction(HFpEF)increases with the ageing of populations.This study aimed to explore ageing-associated gene signatures in HFpEF to develop new diagnostic biomarkers and provide new insights into the underlying mechanisms of HFpEF.Mice were subjected to a high-fat diet combined with L-NG-nitroargi-nine methyl ester(L-NAME)to induce HFpEF,and next-generation sequencing was performed with HFpEF hearts.Additionally,separate datasets were acquired from the Gene Expression Omnibus(GEO)database.The differentially expressed genes(DEGs)were used to identify ageing-related DEGs.Support vector machine,random forest,and least absolute shrinkage and selection operator algorithms were employed to identify potential diagnostic genes from ageing-related DEGs.The diagnostic value was assessed using a nomogram and receiver oper-ating characteristic curve.The gene and related protein expression were verified by reverse transcription PCR and western blotting.The immune cell infiltration in hearts was analysed us-ing the single-sample gene-set enrichment analysis algorithm.The results showed that the merged HFpEF datasets comprised 103 genes,of which 15 ageing-related DEGs were further screened in.The ageing-related DEGs were primarily associated with immune and metabolism regulation.AGTR1a,NR3C1,and PRKAB1 were selected for nomogram construction and ma-chine learning-based diagnostic value,displaying strong diagnostic potential.Additionally,ageing scores were established based on nine key DEGs,revealing noteworthy differences in immune cell infiltration across HFpEF subtypes.In summary,those results highlight the signif-icance of immune dysfunction in HFpEF.Furthermore,ageing-related DEGs might serve as promising prognostic and predictive biomarkers for HFpEF.展开更多
Sodium-ion batteries(SIBs)are the promising rechargeable batteries in large-scale energy storage systems for their low cost,high safety,wide temperature range adaptability,environmental friendliness and excellent fast...Sodium-ion batteries(SIBs)are the promising rechargeable batteries in large-scale energy storage systems for their low cost,high safety,wide temperature range adaptability,environmental friendliness and excellent fast-charging capabilities.Significant research endeavors in SIBs have focused on the exploration of high-performance electrode materials and thorough investigation of their mechanisms.Na_(2)FePO_(4)F(NFPF)is one of potential cathode materials because of low cost,minimal volume strain and extended cycle performance.This review summarizes the crystal structure,sodium ion migration pathways,and synthesis methods of NFPF and discusses the effect of various strategies including hybridization with carbon materials,ion doping,morphology control and electrolyte optimization on its electrochemical performance.Additionally,the application of the NFPF in different batteries is summarized.Finally,the challenges and future directions of NFPF are proposed.This review is both timely and important for promoting the applications of cost-effective NFPF.展开更多
In this paper, by virtue of Leray-Lions theorem, we are mainly concerned with the existence of weak solutions to a Dirichlet boundary value problem with the p-Laplacian operator.
基金supported by grants from the National Natural Science Foundation of China(No.82070238)the Natural Science Foundation of Chongqing,China(No.CSTB2022NSCQ-MSX0913,CSTB2023NSCQ-MSX0219,CSTC2020JCYJ-MSXM X0290)+1 种基金the Chongqing Education Committee of China(No.KJQN202300480)the Program for Youth Innovation in Future Medicine,Chongqing Medical University(No.W0168).
文摘The incidence of heart failure with preserved ejection fraction(HFpEF)increases with the ageing of populations.This study aimed to explore ageing-associated gene signatures in HFpEF to develop new diagnostic biomarkers and provide new insights into the underlying mechanisms of HFpEF.Mice were subjected to a high-fat diet combined with L-NG-nitroargi-nine methyl ester(L-NAME)to induce HFpEF,and next-generation sequencing was performed with HFpEF hearts.Additionally,separate datasets were acquired from the Gene Expression Omnibus(GEO)database.The differentially expressed genes(DEGs)were used to identify ageing-related DEGs.Support vector machine,random forest,and least absolute shrinkage and selection operator algorithms were employed to identify potential diagnostic genes from ageing-related DEGs.The diagnostic value was assessed using a nomogram and receiver oper-ating characteristic curve.The gene and related protein expression were verified by reverse transcription PCR and western blotting.The immune cell infiltration in hearts was analysed us-ing the single-sample gene-set enrichment analysis algorithm.The results showed that the merged HFpEF datasets comprised 103 genes,of which 15 ageing-related DEGs were further screened in.The ageing-related DEGs were primarily associated with immune and metabolism regulation.AGTR1a,NR3C1,and PRKAB1 were selected for nomogram construction and ma-chine learning-based diagnostic value,displaying strong diagnostic potential.Additionally,ageing scores were established based on nine key DEGs,revealing noteworthy differences in immune cell infiltration across HFpEF subtypes.In summary,those results highlight the signif-icance of immune dysfunction in HFpEF.Furthermore,ageing-related DEGs might serve as promising prognostic and predictive biomarkers for HFpEF.
基金supported by National Natural Science Foundation of China(No.52064031)Natural Science Foundation of Yunnan Province(Nos.202301BE070001–014,202301AT070150,202402AB080001)the Analysis and Testing Foundation of Kunming University of Science and Technology(No.2022T20210182)。
文摘Sodium-ion batteries(SIBs)are the promising rechargeable batteries in large-scale energy storage systems for their low cost,high safety,wide temperature range adaptability,environmental friendliness and excellent fast-charging capabilities.Significant research endeavors in SIBs have focused on the exploration of high-performance electrode materials and thorough investigation of their mechanisms.Na_(2)FePO_(4)F(NFPF)is one of potential cathode materials because of low cost,minimal volume strain and extended cycle performance.This review summarizes the crystal structure,sodium ion migration pathways,and synthesis methods of NFPF and discusses the effect of various strategies including hybridization with carbon materials,ion doping,morphology control and electrolyte optimization on its electrochemical performance.Additionally,the application of the NFPF in different batteries is summarized.Finally,the challenges and future directions of NFPF are proposed.This review is both timely and important for promoting the applications of cost-effective NFPF.
基金supported by the NNSF of China(10971046)Shandong Provincial Natural Science Foundation(ZR2012AQ007)The Project of Shandong Province High Education Science and Tech-nology Program(J09LA55)
文摘In this paper, by virtue of Leray-Lions theorem, we are mainly concerned with the existence of weak solutions to a Dirichlet boundary value problem with the p-Laplacian operator.
