Addressing peripheral nerve defects remains a significant challenge in regenerative neurobiology.Autograftsemerged as the gold-standard management,however,are hindered by limited availability and potential neuromaform...Addressing peripheral nerve defects remains a significant challenge in regenerative neurobiology.Autograftsemerged as the gold-standard management,however,are hindered by limited availability and potential neuromaformation.Numerous recent studies report the potential of wireless electronic system for nerve defects repair.Unfortunately,few has met clinical needs for inadequate electrode precision,poor nerve entrapment andinsufficient bioactivity of the matrix material.Herein,we present an advanced wireless electrical nerve stimulator,based on water-responsive self-curling silk membrane with excellent bioabsorbable and biocompatibleproperties.We constructed a unique bilayer structure with an oriented pre-stretched inner layer and a generalsilk membrane as outer layer.After wetting,the simultaneous contraction of inner layer and expansion of outerlayer achieved controllable super-contraction from 2D flat surface to 3D structural reconfiguration.It enablesshape-adaptive wrapping to cover around nerves,overcomes the technical obstacle of preparing electrodes on theinner wall of the conduit,and prevents electrode breakage caused by material expansion in water.The use of forkcapacitor-like metal interface increases the contact points between the metal and the regenerating nerve,solvingthe challenge of inefficient and rough electrical stimulation methods in the past.Newly developed electronicstimulator is effective in restoring 10 mm rat sciatic nerve defects comparable to autologous grafts.The underlyingmechanism involves that electric stimulation enhances anterograde mitochondrial transport to matchenergy demands.This newly introduced device thereby demonstrated the potential as a viable and efficaciousalternative to autografts for enhancing peripheral nerve repair and functional recovery.展开更多
Blood viscosity changes and blood clots are high-impact diseases,but the pathogenic mechanisms and detection methods are still limited.Due to the complexity of the cellular microenvironment,viscosity is a key factor i...Blood viscosity changes and blood clots are high-impact diseases,but the pathogenic mechanisms and detection methods are still limited.Due to the complexity of the cellular microenvironment,viscosity is a key factor in regulating the behavior of mitochondria and lysosomes in cells.Conventional fluorescence probes are highly restrictive for complex viscosity detection in live animals.Therefore,we developed two nearinfrared fluorescence probes,QL1 and QL2,with dual responses to the pH and viscosity.Notably,QL2 has two maximum fluorescence emissions at 680 and 750 nm,when excitation by 580 and 700 nm,respectively.QL2 exhibited both a pH and viscosity switchable fluorescence response.The two emission peaks exhibited a reverse change trend:the fluorescence at 680 nm decreased by 90%,and the fluorescence at 750 nm increased by about 5-fold with pH from 2 to 10.Meanwhile,both emission peaks show remarkable fluorescence enhancement toward viscosity change,with 185 and 32 times enhancement,respectively.The sensing mechanism and spectral changes are confirmed by DFT calculations.QL2 was further used for viscosity imaging in live cells,zebrafish,and live animals.Most importantly,QL2 is able to successfully track changes in blood clots in live mice and organs,thus enabling the study of blood clots in cerebral strokes and the underlying pathological mechanisms.展开更多
基金supported by the National Natural Science Foundation of China(82172476,82172393).
文摘Addressing peripheral nerve defects remains a significant challenge in regenerative neurobiology.Autograftsemerged as the gold-standard management,however,are hindered by limited availability and potential neuromaformation.Numerous recent studies report the potential of wireless electronic system for nerve defects repair.Unfortunately,few has met clinical needs for inadequate electrode precision,poor nerve entrapment andinsufficient bioactivity of the matrix material.Herein,we present an advanced wireless electrical nerve stimulator,based on water-responsive self-curling silk membrane with excellent bioabsorbable and biocompatibleproperties.We constructed a unique bilayer structure with an oriented pre-stretched inner layer and a generalsilk membrane as outer layer.After wetting,the simultaneous contraction of inner layer and expansion of outerlayer achieved controllable super-contraction from 2D flat surface to 3D structural reconfiguration.It enablesshape-adaptive wrapping to cover around nerves,overcomes the technical obstacle of preparing electrodes on theinner wall of the conduit,and prevents electrode breakage caused by material expansion in water.The use of forkcapacitor-like metal interface increases the contact points between the metal and the regenerating nerve,solvingthe challenge of inefficient and rough electrical stimulation methods in the past.Newly developed electronicstimulator is effective in restoring 10 mm rat sciatic nerve defects comparable to autologous grafts.The underlyingmechanism involves that electric stimulation enhances anterograde mitochondrial transport to matchenergy demands.This newly introduced device thereby demonstrated the potential as a viable and efficaciousalternative to autografts for enhancing peripheral nerve repair and functional recovery.
基金supported by the Shandong Province Natural Science Foundation(ZR202306050008)Innovation Team Project of 20 items-university of Jinan(202228073)+2 种基金NSFC(No.31971605)the Pilot Project for Integrating Science,Education and Industry(2022PYI007)the Taishan Scholars Program.
文摘Blood viscosity changes and blood clots are high-impact diseases,but the pathogenic mechanisms and detection methods are still limited.Due to the complexity of the cellular microenvironment,viscosity is a key factor in regulating the behavior of mitochondria and lysosomes in cells.Conventional fluorescence probes are highly restrictive for complex viscosity detection in live animals.Therefore,we developed two nearinfrared fluorescence probes,QL1 and QL2,with dual responses to the pH and viscosity.Notably,QL2 has two maximum fluorescence emissions at 680 and 750 nm,when excitation by 580 and 700 nm,respectively.QL2 exhibited both a pH and viscosity switchable fluorescence response.The two emission peaks exhibited a reverse change trend:the fluorescence at 680 nm decreased by 90%,and the fluorescence at 750 nm increased by about 5-fold with pH from 2 to 10.Meanwhile,both emission peaks show remarkable fluorescence enhancement toward viscosity change,with 185 and 32 times enhancement,respectively.The sensing mechanism and spectral changes are confirmed by DFT calculations.QL2 was further used for viscosity imaging in live cells,zebrafish,and live animals.Most importantly,QL2 is able to successfully track changes in blood clots in live mice and organs,thus enabling the study of blood clots in cerebral strokes and the underlying pathological mechanisms.
