[Objectives]This study was conducted to investigate the inhibitory effects of different extraction fractions of Baeckea frutescens L.on the proliferation of human colon cancer LOVO cells in vitro.[Methods]Leaves were ...[Objectives]This study was conducted to investigate the inhibitory effects of different extraction fractions of Baeckea frutescens L.on the proliferation of human colon cancer LOVO cells in vitro.[Methods]Leaves were separated from the aerial part of B.frutescens,immersed in distilled water and treated by ultrasound for half an hour.The volatile oil in B.frutescens leaves was extracted by steam distillation.The leaves after removing the volatile oil were extracted by reflux condensation,and then the extract was concentrated into fluid extract.The organic solvent extraction method was applied to extract the fluid extract until the organic phase was nearly colorless,and the extracts were combined,and concentrated into fluid extract,which was then freeze-dried into powder for later use.A cytotoxicity experiment was carried out on human colon cancer LOVO cells with different extraction fractions of B.frutescens as test drugs.Cell activity was detected by MTT assay.[Results]The petroleum ether fraction and chloroform fraction of B.frutescens had no inhibitory effect on the proliferation of human intestinal cancer LOVO cells in vitro.The ethyl acetate fraction and n-butanol fraction of B.frutescens had inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner.The IC_(50)of ethyl acetate was 398.94μg/ml,and that of n-butanol was 617.02μg/ml.[Conclusions]The ethyl acetate and n-butanol extraction fractions from B.frutescens have inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner.展开更多
[Objectives]This study was conducted to explore the proliferation inhibition of hirudin on hepatocellular carcinoma HepG 2 cells and Huh-7 cells.[Methods]Hirudin solutions of different concentrations(2.0,2.5,3.0,3.5,4...[Objectives]This study was conducted to explore the proliferation inhibition of hirudin on hepatocellular carcinoma HepG 2 cells and Huh-7 cells.[Methods]Hirudin solutions of different concentrations(2.0,2.5,3.0,3.5,4.0 mg/ml)were used to treat HepG 2 cells and Huh-7 cells.The effects of different hirudin concentrations on the proliferative activity of HepG2 and Huh-7 cells were detected by CCK-8 assay,and the IC 50 values were calculated.A living/dead cell double staining experiment was conducted to observe the fluorescence of cells under a fluorescent microscope,so as to assess the inhibitory effect of different concentrations of hirudin on the proliferation of HepG2 and Huh-7 cells.A cell scratch assay was carried out,and an inverted microscope was employed to observe the healing of the scratched areas,so as to assess the impact of hirudin on the migratory and invasive capabilities of hepatocellular carcinoma HepG2 and Huh-7 cells.[Results](i)The results of CCK-8 assay indicated that compared with the blank control group,the proliferation inhibition rates of both hepatocellular carcinoma HepG2 and Huh-7 cells increased with the concentration of hirudin increasing,demonstrating that hirudin had an inhibitory effect on the proliferative activity of these cells.Specifically,the IC 50 values for HepG2 and Huh-7 were found to be 3.5 and 4.0 mg/ml.(ii)The living/dead cell double staining experiment revealed that the number of living cells in the hirudin-treated group decreased significantly compared with the control group,while the number of dead cells increased markedly,indicating an inhibitory effect of hirudin on the proliferation of HepG2 and Huh-7 cells.(iii)The results of cell scratch assay showed that the healing degree of the scratched areas in the hirudin-treated groups was significantly lower than that of the control group,indicating a reduction in cell growth and migration capabilities.[Conclusions]Hirudin exhibited a significant inhibitory effect on the proliferation of hepatocellular carcinoma HepG2 and Huh-7 cells.展开更多
基金Supported by Social Development Program of Guilin Science and Technology Bureau(20210227-9-3)Guangxi Medical and Health Appropriate Technology Development and Application of the Project(S202310601143)University-Level Undergraduate Innovation and Entrepreneurship Training Program(X202410601223).
文摘[Objectives]This study was conducted to investigate the inhibitory effects of different extraction fractions of Baeckea frutescens L.on the proliferation of human colon cancer LOVO cells in vitro.[Methods]Leaves were separated from the aerial part of B.frutescens,immersed in distilled water and treated by ultrasound for half an hour.The volatile oil in B.frutescens leaves was extracted by steam distillation.The leaves after removing the volatile oil were extracted by reflux condensation,and then the extract was concentrated into fluid extract.The organic solvent extraction method was applied to extract the fluid extract until the organic phase was nearly colorless,and the extracts were combined,and concentrated into fluid extract,which was then freeze-dried into powder for later use.A cytotoxicity experiment was carried out on human colon cancer LOVO cells with different extraction fractions of B.frutescens as test drugs.Cell activity was detected by MTT assay.[Results]The petroleum ether fraction and chloroform fraction of B.frutescens had no inhibitory effect on the proliferation of human intestinal cancer LOVO cells in vitro.The ethyl acetate fraction and n-butanol fraction of B.frutescens had inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner.The IC_(50)of ethyl acetate was 398.94μg/ml,and that of n-butanol was 617.02μg/ml.[Conclusions]The ethyl acetate and n-butanol extraction fractions from B.frutescens have inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner.
基金Social Development Program of Guilin Science and Technology Bureau(20210227-9-3)Guangxi Medical and Health Appropriate Technology Development and Application Project(S2021057)School-level Undergraduate Innovation and Entrepreneurship Training Program(X2023106012040).
文摘[Objectives]This study was conducted to explore the proliferation inhibition of hirudin on hepatocellular carcinoma HepG 2 cells and Huh-7 cells.[Methods]Hirudin solutions of different concentrations(2.0,2.5,3.0,3.5,4.0 mg/ml)were used to treat HepG 2 cells and Huh-7 cells.The effects of different hirudin concentrations on the proliferative activity of HepG2 and Huh-7 cells were detected by CCK-8 assay,and the IC 50 values were calculated.A living/dead cell double staining experiment was conducted to observe the fluorescence of cells under a fluorescent microscope,so as to assess the inhibitory effect of different concentrations of hirudin on the proliferation of HepG2 and Huh-7 cells.A cell scratch assay was carried out,and an inverted microscope was employed to observe the healing of the scratched areas,so as to assess the impact of hirudin on the migratory and invasive capabilities of hepatocellular carcinoma HepG2 and Huh-7 cells.[Results](i)The results of CCK-8 assay indicated that compared with the blank control group,the proliferation inhibition rates of both hepatocellular carcinoma HepG2 and Huh-7 cells increased with the concentration of hirudin increasing,demonstrating that hirudin had an inhibitory effect on the proliferative activity of these cells.Specifically,the IC 50 values for HepG2 and Huh-7 were found to be 3.5 and 4.0 mg/ml.(ii)The living/dead cell double staining experiment revealed that the number of living cells in the hirudin-treated group decreased significantly compared with the control group,while the number of dead cells increased markedly,indicating an inhibitory effect of hirudin on the proliferation of HepG2 and Huh-7 cells.(iii)The results of cell scratch assay showed that the healing degree of the scratched areas in the hirudin-treated groups was significantly lower than that of the control group,indicating a reduction in cell growth and migration capabilities.[Conclusions]Hirudin exhibited a significant inhibitory effect on the proliferation of hepatocellular carcinoma HepG2 and Huh-7 cells.
