Temperature is an essential environmental factor affecting the viability of organisms.Temperature changes could cause damages or even death.The oriental river prawn,Macrobrachium nipponense,is widely distributed in Ea...Temperature is an essential environmental factor affecting the viability of organisms.Temperature changes could cause damages or even death.The oriental river prawn,Macrobrachium nipponense,is widely distributed in East Asian countries,and has been an important economical aquaculture species in China since 1990s.This prawn belongs to crustaceans,which is a kind of ectotherms and very sensitive to temperature changes due to their relatively low internal metabolic heat production and their high thermal conductivity.In order to reveal the thermal adaptation mechanisms,M.nipponense were acclimated at 25℃ for 24 h,and successively challenged at 30℃ and 35℃ for 24 h in the same tank,respectively.And histological,biochemical and transcriptomic analyses were carried out on the gills and hepatopancreas under different temperatures.The results showed that different degrees of histological damages were discovered both in the gills and hepatopancreas of M.nipponense at 30℃ and 35℃.The digestive,metabolic,and antioxidative capacity were enhanced in the gills and hepatopancreas of M.nipponense at 30℃ and 35℃.A total of 2293 and 3304 differentially expressed genes(DEGs)were identified from the gills and hepatopancreas,respectively.These DEGs were significantly involved in the regulation of transcription,proteolysis,nucleus,cytoplasm,metal ion binding,and ATP binding by Gene ontology(GO)enrichment analysis.Furthermore,the DEGs in the hepatopancreas were significantly enriched in KEGG pathways including neuroactive ligand-receptor interaction,thyroid hormone synthesis,and ECM-receptor interaction.And the DEGs in the gills were enriched in KEGG pathways including cGMP-PKG signaling pathway,ribosome,and calcium signaling pathway.These results could be helpful for further understanding the molecular mechanisms underlying the thermal response of M.nipponense,and increasing knowledge of the regulation mechanisms of freshwater crustaceans in the context of global warming.展开更多
The giant freshwater prawn(Macrobrachium rosenbergii)is a commercially cultured species in China.A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes.Here,based on the ...The giant freshwater prawn(Macrobrachium rosenbergii)is a commercially cultured species in China.A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes.Here,based on the results of reduced-representation genome sequencing and genome survey of M.rosenbergii,we identified steady sex-linked markers.Further nucleotide alignment analysis revealed 83.42%nucleotide similarity between the amplified W-and Z-chromosome fragments.qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females.Similarly,the W chromosome-specific fragments were present in super females approximately twice as often compared to females.Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M.rosenbergii.展开更多
Grass carp has the highest production yield among all the farmed fish species around the world.But the molecular and genetic basis of growth traits is inadequately understood in this fish.In the present study,we apply...Grass carp has the highest production yield among all the farmed fish species around the world.But the molecular and genetic basis of growth traits is inadequately understood in this fish.In the present study,we apply whole-transcriptome sequencing to identify genes whose expression is different between fast and slow growing grass carp.We identified 1178 differentially expressed genes(DEGs),among which 270 were upregulated in the fast growing group and 908 upregulated in the slow growing group.The GO enrichment analysis revealed that DEGs was significantly enriched in protein synthesis and export machinery,cell division and development and metabolism,suggesting that cell division and metabolism may play key roles in development processes of fish.KEGG pathway analysis showed that MAPK signaling pathway may play a prominent role in growth difference.In addition,the majority of genes in immune responses related pathway were upregulated in the slow growing individuals.These results provide some valuable resources for understanding the molecular mechanisms of growth traits in fish.展开更多
基金supported by the Innovation Action Plan Project of the Science and Technology Commission of Shanghai Municipality,China(19391900900,21002410500).
文摘Temperature is an essential environmental factor affecting the viability of organisms.Temperature changes could cause damages or even death.The oriental river prawn,Macrobrachium nipponense,is widely distributed in East Asian countries,and has been an important economical aquaculture species in China since 1990s.This prawn belongs to crustaceans,which is a kind of ectotherms and very sensitive to temperature changes due to their relatively low internal metabolic heat production and their high thermal conductivity.In order to reveal the thermal adaptation mechanisms,M.nipponense were acclimated at 25℃ for 24 h,and successively challenged at 30℃ and 35℃ for 24 h in the same tank,respectively.And histological,biochemical and transcriptomic analyses were carried out on the gills and hepatopancreas under different temperatures.The results showed that different degrees of histological damages were discovered both in the gills and hepatopancreas of M.nipponense at 30℃ and 35℃.The digestive,metabolic,and antioxidative capacity were enhanced in the gills and hepatopancreas of M.nipponense at 30℃ and 35℃.A total of 2293 and 3304 differentially expressed genes(DEGs)were identified from the gills and hepatopancreas,respectively.These DEGs were significantly involved in the regulation of transcription,proteolysis,nucleus,cytoplasm,metal ion binding,and ATP binding by Gene ontology(GO)enrichment analysis.Furthermore,the DEGs in the hepatopancreas were significantly enriched in KEGG pathways including neuroactive ligand-receptor interaction,thyroid hormone synthesis,and ECM-receptor interaction.And the DEGs in the gills were enriched in KEGG pathways including cGMP-PKG signaling pathway,ribosome,and calcium signaling pathway.These results could be helpful for further understanding the molecular mechanisms underlying the thermal response of M.nipponense,and increasing knowledge of the regulation mechanisms of freshwater crustaceans in the context of global warming.
基金This work was supported by the National Key R&D Program of China(project number 2018YFD0900201)the Natural Science Foundation of China(project number 31772841 and 31902348)the Shanghai Sailing Program(project number 17YF1408000).
文摘The giant freshwater prawn(Macrobrachium rosenbergii)is a commercially cultured species in China.A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes.Here,based on the results of reduced-representation genome sequencing and genome survey of M.rosenbergii,we identified steady sex-linked markers.Further nucleotide alignment analysis revealed 83.42%nucleotide similarity between the amplified W-and Z-chromosome fragments.qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females.Similarly,the W chromosome-specific fragments were present in super females approximately twice as often compared to females.Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M.rosenbergii.
基金the Project of Shanghai Engineering and Technology Center for Promoting Ability(19DZ2284300)the Earmarked Fund for the China Agriculture Research System(CARS-45-03)。
文摘Grass carp has the highest production yield among all the farmed fish species around the world.But the molecular and genetic basis of growth traits is inadequately understood in this fish.In the present study,we apply whole-transcriptome sequencing to identify genes whose expression is different between fast and slow growing grass carp.We identified 1178 differentially expressed genes(DEGs),among which 270 were upregulated in the fast growing group and 908 upregulated in the slow growing group.The GO enrichment analysis revealed that DEGs was significantly enriched in protein synthesis and export machinery,cell division and development and metabolism,suggesting that cell division and metabolism may play key roles in development processes of fish.KEGG pathway analysis showed that MAPK signaling pathway may play a prominent role in growth difference.In addition,the majority of genes in immune responses related pathway were upregulated in the slow growing individuals.These results provide some valuable resources for understanding the molecular mechanisms of growth traits in fish.
