Coenzyme Q_(10)(CoQ_(10))is a crucial endogenous lipophilic antioxidant,and encapsulation is often used to improve its solubility and stability.However,information about the bioavailability and safety assessment of co...Coenzyme Q_(10)(CoQ_(10))is a crucial endogenous lipophilic antioxidant,and encapsulation is often used to improve its solubility and stability.However,information about the bioavailability and safety assessment of constructed formulations is limited.In this study,in vivo pharmacokinetics profile and sub-chronic toxicity of CoQ_(10) loaded whey protein concentrate(WPC)and polymerized whey protein concentrate(PWPC)nanoparticles were evaluated.Pharmacokinetics data showed that CoQ_(10) concentrations in the plasma of rats fed with nanoparticles were higher than that of the free counterpart.CoQ_(10) concentration reached its maximum level after 12 h when orally administered with WPC and PWPC based encapsulation,which was delayed compared with free CoQ_(10)(8 h).Sub-chronic toxicity evaluation for PWPC-CoQ_(10) was conducted with 28-day feeding test.Results indicated that no adverse toxicological reactions in term of physical examination,body weight,food intake,hematology,serum chemistry,organ weight or histopathology excepted for blood potassium were observed after oral administration of feed with incorporated PWPC-CoQ_(10) nanoparticles.The maximum concentration of nanoparticle powder in diet of no adverse effect on the male and female rats was up to 2%(w/w).Results denoted a potential application of whey protein as a safe natural polymer to fabricate delivery systems to enhance bioavailability of nutritional supplements like CoQ_(10).展开更多
文摘Coenzyme Q_(10)(CoQ_(10))is a crucial endogenous lipophilic antioxidant,and encapsulation is often used to improve its solubility and stability.However,information about the bioavailability and safety assessment of constructed formulations is limited.In this study,in vivo pharmacokinetics profile and sub-chronic toxicity of CoQ_(10) loaded whey protein concentrate(WPC)and polymerized whey protein concentrate(PWPC)nanoparticles were evaluated.Pharmacokinetics data showed that CoQ_(10) concentrations in the plasma of rats fed with nanoparticles were higher than that of the free counterpart.CoQ_(10) concentration reached its maximum level after 12 h when orally administered with WPC and PWPC based encapsulation,which was delayed compared with free CoQ_(10)(8 h).Sub-chronic toxicity evaluation for PWPC-CoQ_(10) was conducted with 28-day feeding test.Results indicated that no adverse toxicological reactions in term of physical examination,body weight,food intake,hematology,serum chemistry,organ weight or histopathology excepted for blood potassium were observed after oral administration of feed with incorporated PWPC-CoQ_(10) nanoparticles.The maximum concentration of nanoparticle powder in diet of no adverse effect on the male and female rats was up to 2%(w/w).Results denoted a potential application of whey protein as a safe natural polymer to fabricate delivery systems to enhance bioavailability of nutritional supplements like CoQ_(10).
