Interbody fusion is recognized as the golden standard of surgical intervention for degenerative disc disease(DDD).Interbody fusion cage made of polyetheretherketone(PEEK)is commonly used in lumbar interbody fusion sur...Interbody fusion is recognized as the golden standard of surgical intervention for degenerative disc disease(DDD).Interbody fusion cage made of polyetheretherketone(PEEK)is commonly used in lumbar interbody fusion surgery in the treatment of DDD worldwide.However,there are some limitations of PEEK including their bio-inert nature and impediment to host bone integration.This study aimed to evaluate the degradation profile and osteoinductive potential of biodegradable Mg-Zn-Nd-Zr cages with/without micro-arc oxidation(MAO)coatings.The Mg-Zn-Nd-Zr alloy cages,whether coated with MAO or not,demonstrated commendable biocompatibility and biomechanical properties.Immersion and electrochemical tests show better corrosion resistance of MAO coatings in vitro.mRNA sequencing,RT-qPCR and Western blotting revealed that Mg-Zn-Nd-Zr and Mg-Zn-Nd-Zr/MAO had a better effectiveness on osteoinductivity.In vivo evaluations in ovine models over 12 weeks and 24 weeks post-implantation revealed radiological and histological evidence of enhanced bone formation adjacent to the Mg-Zn-Nd-Zr alloy cages compared to PEEK counterparts.Moreover,the MAO-coated cages exhibited a reduced propensity for gas formation.The Mg-Zn-Nd-Zr alloy is as a superior osteoinductive material compared with PEEK,with the MAO coating offering an advantage in mitigating gas production.Nonetheless,further research is warranted to refine the alloy's composition or surface treatments,particularly to address the challenges associated with rapid gas evolution during the early post-implantation period.展开更多
The incidence of open bone defects caused by high kinetic and potential energy injuries has significantly increased.Bone grafting,typically in the form of granules,is widely recognized as the most effective treatment....The incidence of open bone defects caused by high kinetic and potential energy injuries has significantly increased.Bone grafting,typically in the form of granules,is widely recognized as the most effective treatment.However,current bone graft system is not considered ideal due to issues such as mismatched shapes and dislocation.Additionally,bone defects are frequently associated with substantial bleeding,and bone graft system often fail to effectively seal and prevent leakage,increasing the post-operative complications.In this study,based on PEG active ester(Bi-PEG-SG)and gelatin,we developed a micro-scale calf bone granules/PEG-Gelatin bio-adhesive grafts delivery system with self-healing properties,which not only possesses antioxidant properties but also demonstrates injectability,shape adaptability,adhesive capabilities and high bursting pressure.This system effectively addresses the displacement issues of bone grafts and shows significant sealing and hemostatic ca-pabilities in models of femoral artery transection hemorrhage and rabbit femoral condyle bleeding.Furthermore,the bone/bioadhesive graft delivery system serves as a sustained-release carrier for vancomycin and recombinant human bone morphogenetic protein-2,demonstrating good antibacterial performance and enhancing the osteoinductive activity and osteogenic microenvironment of calf bone granules,thereby promoting the repair of bone defects.Overall,this system offers a promising alternative for the fabrication of bone granules delivery system,demonstrating significant potential as a treatment option for open bone defects.展开更多
基金supported by the National Natural Science Foundation of China(82172392)the National Natural Science Foundation of China(82372401)+1 种基金the Beijing Natural Science Foundation(L202033)the Basic Applied Research Program of Liaoning Province of China(2022020347-JH2/1013)。
文摘Interbody fusion is recognized as the golden standard of surgical intervention for degenerative disc disease(DDD).Interbody fusion cage made of polyetheretherketone(PEEK)is commonly used in lumbar interbody fusion surgery in the treatment of DDD worldwide.However,there are some limitations of PEEK including their bio-inert nature and impediment to host bone integration.This study aimed to evaluate the degradation profile and osteoinductive potential of biodegradable Mg-Zn-Nd-Zr cages with/without micro-arc oxidation(MAO)coatings.The Mg-Zn-Nd-Zr alloy cages,whether coated with MAO or not,demonstrated commendable biocompatibility and biomechanical properties.Immersion and electrochemical tests show better corrosion resistance of MAO coatings in vitro.mRNA sequencing,RT-qPCR and Western blotting revealed that Mg-Zn-Nd-Zr and Mg-Zn-Nd-Zr/MAO had a better effectiveness on osteoinductivity.In vivo evaluations in ovine models over 12 weeks and 24 weeks post-implantation revealed radiological and histological evidence of enhanced bone formation adjacent to the Mg-Zn-Nd-Zr alloy cages compared to PEEK counterparts.Moreover,the MAO-coated cages exhibited a reduced propensity for gas formation.The Mg-Zn-Nd-Zr alloy is as a superior osteoinductive material compared with PEEK,with the MAO coating offering an advantage in mitigating gas production.Nonetheless,further research is warranted to refine the alloy's composition or surface treatments,particularly to address the challenges associated with rapid gas evolution during the early post-implantation period.
基金supported by the National Natural Science Foundation of China(No.82372401,52103184)the Beijing Natural Science Foundation(No.L202033)+1 种基金the Key Program of the National Natural Science Foundation of China(No.21935011)the Funding for Basic Scientific Research and“Young Talent Support Plan”of Xi’an Jiaotong University(xzy012022038).
文摘The incidence of open bone defects caused by high kinetic and potential energy injuries has significantly increased.Bone grafting,typically in the form of granules,is widely recognized as the most effective treatment.However,current bone graft system is not considered ideal due to issues such as mismatched shapes and dislocation.Additionally,bone defects are frequently associated with substantial bleeding,and bone graft system often fail to effectively seal and prevent leakage,increasing the post-operative complications.In this study,based on PEG active ester(Bi-PEG-SG)and gelatin,we developed a micro-scale calf bone granules/PEG-Gelatin bio-adhesive grafts delivery system with self-healing properties,which not only possesses antioxidant properties but also demonstrates injectability,shape adaptability,adhesive capabilities and high bursting pressure.This system effectively addresses the displacement issues of bone grafts and shows significant sealing and hemostatic ca-pabilities in models of femoral artery transection hemorrhage and rabbit femoral condyle bleeding.Furthermore,the bone/bioadhesive graft delivery system serves as a sustained-release carrier for vancomycin and recombinant human bone morphogenetic protein-2,demonstrating good antibacterial performance and enhancing the osteoinductive activity and osteogenic microenvironment of calf bone granules,thereby promoting the repair of bone defects.Overall,this system offers a promising alternative for the fabrication of bone granules delivery system,demonstrating significant potential as a treatment option for open bone defects.
