Tissues are made up of cells and the extracellular matrix(ECM)which surrounds them.These cells and tissues are actively adaptable to enduring significant stress that occurs in daily life.This astonishing mechanical st...Tissues are made up of cells and the extracellular matrix(ECM)which surrounds them.These cells and tissues are actively adaptable to enduring significant stress that occurs in daily life.This astonishing mechanical stress develops due to the interaction between the live cells and the non-living ECM.Cells in the matrix microenvironment can sense the signals and forces produced and initiate a signaling cascade that plays a crucial role in the body’s normal functioning and influences various properties of the native cells,including growth,proliferation,and differentiation.However,the matrix’s characteristic features also impact the repair and regeneration of the damaged tissues.The current study reviewed how the cell-ECM interaction regulates cellular behavior and physicochemical properties.Herein,we have described the response of cells to mechanical stresses,the importance of substrate stiffness and geometry in tissue regeneration,and the development of scaffolds to mimic the nature of native ECM in 3D for tissue engineering applications has also been discussed.Finally,the study summarizes the conclusions and promising prospects based on the cell-ECM interplay.展开更多
Mesenchymal stem cells(MSCs)are multipotent cells usually isolated from bone marrow,endometrium,adipose tissues,skin,and dental pulp.MSCs played a crucial role in regenerative therapy and have been introduced as an in...Mesenchymal stem cells(MSCs)are multipotent cells usually isolated from bone marrow,endometrium,adipose tissues,skin,and dental pulp.MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science.Recently,MSCs have been widely explored for their application in regenerative medicine and COVID-19 treatment.Different approaches to evaluate the future of biomaterials and stem cell properties have been developed.However,misconceptions and ethical issues still exist,such as MSCs being non-angiogenic,anti-apoptotic,and immunoregulatory competencies.Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs.These issues generate questions related to ethical and moral issues.However,MSCs have gained considerable attention for tissue regeneration owing to their differentiation ability with immunomodulatory effects.They are capable of secreting a broad range of biomolecules such as proteins,nucleic acids,exosomes,microRNAs,and membrane vesicles,collectively known as secretomes.Secretomes are released in response to the surrounding microenvironment.In this article,we briefly address topics related to the therapeutic potential of MSCs as an advanced approach in the field of regenerative medicine and various perspectives.展开更多
Biomaterial composition and surface charge play a critical role in macrophage polarization,providing a mo-lecular cue for immunomodulation and tissue regeneration.In this study,we developed bifunctional hydrogel inks ...Biomaterial composition and surface charge play a critical role in macrophage polarization,providing a mo-lecular cue for immunomodulation and tissue regeneration.In this study,we developed bifunctional hydrogel inks for accelerating M2 macrophage polarization and exosome(Exo)cultivation for wound healing applications.For this,we first fabricated polyamine-modified three-dimensional(3D)printable hydrogels consisting of algi-nate/gelatin/polydopamine nanospheres(AG/NSPs)to boost M2-exosome(M2-Exo)secretion.The cultivated M2-Exo were finally encapsulated into a biocompatible collagen/decellularized extracellular matrix(COL@d-ECM)bioink for studying angiogenesis and in vivo wound healing study.Our findings show that 3D-printed AGP hydrogel promoted M2 macrophage polarization by Janus kinase/signal transducer of activation(JAK/STAT),peroxisome proliferator-activated receptor(PPAR)signaling pathways and facilitated the M2-Exo secretion.Moreover,the COL@d-ECM/M2-Exo was found to be biocompatible with skin cells.Transcriptomic(RNA-Seq)and real-time PCR(qRT-PCR)study revealed that co-culture of fibroblast/keratinocyte/stem cells/endothelial cells in a 3D bioprinted COL@d-ECM/M2-Exo hydrogel upregulated the skin-associated signature biomarkers through various regulatory pathways during epidermis remodeling and downregulated the mitogen-activated protein kinase(MAPK)signaling pathway after 7 days.In a subcutaneous wound model,the 3D bioprinted COL@d-ECM/M2-Exo hydrogel displayed robust wound remodeling and hair follicle(HF)induction while reducing canonical pro-inflammatory activation after 14 days,presenting a viable therapeutic strategy for skin-related disorders.展开更多
Macrophage-assisted immunomodulation is an alternative strategy in tissue engineering, wherein the interplay between pro-inflammatory and anti-inflammatory macrophage cells and body cells determines the fate of healin...Macrophage-assisted immunomodulation is an alternative strategy in tissue engineering, wherein the interplay between pro-inflammatory and anti-inflammatory macrophage cells and body cells determines the fate of healing or inflammation. Although several reports have demonstrated that tissue regeneration depends on spatial and temporal regulation of the biophysical or biochemical microenvironment of the biomaterial, the underlying molecular mechanism behind immunomodulation is still under consideration for developing immunomodulatory scaffolds. Currently, most fabricated immunomodulatory platforms reported in the literature show regenerative capabilities of a particular tissue, for example, endogenous tissue (e.g., bone, muscle, heart, kidney, and lungs) or exogenous tissue (e.g., skin and eye). In this review, we briefly introduced the necessity of the 3D immunomodulatory scaffolds and nanomaterials, focusing on material properties and their interaction with macrophages for general readers. This review also provides a comprehensive summary of macrophage origin and taxonomy, their diverse functions, and various signal transduction pathways during biomaterial-macrophage interaction, which is particularly helpful for material scientists and clinicians for developing next-generation immunomodulatory scaffolds. From a clinical standpoint, we briefly discussed the role of 3D biomaterial scaffolds and/or nanomaterial composites for macrophage-assisted tissue engineering with a special focus on bone and associated tissues. Finally, a summary with expert opinion is presented to address the challenges and future necessity of 3D bioprinted immunomodulatory materials for tissue engineering.展开更多
Nanocellulose,a biopolymer,has received wide attention from researchers owing to its superior physicochemical properties,such as high mechanical strength,low density,biodegradability,and biocompatibility.Nanocellulose...Nanocellulose,a biopolymer,has received wide attention from researchers owing to its superior physicochemical properties,such as high mechanical strength,low density,biodegradability,and biocompatibility.Nanocellulose can be extracted from wide range of sources,including plants,bacteria,and algae.Depending on the extraction process and dimensions(diameter and length),they are categorized into three main types:cellulose nanocrystals(CNCs),cellulose nanofibrils(CNFs),and bacterial nanocellulose(BNC).CNCs are a highly crystalline and needle-like structure,whereas CNFs have both amorphous and crystalline regions in their network.BNC is the purest form of nanocellulose.The nanocellulose properties can be tuned by chemical functionalization,which increases its applicability in biomedical applications.This review highlights the fabrication of different surface-modified nanocellulose to deliver active molecules,such as drugs,proteins,and plasmids.Nanocellulose-mediated delivery of active molecules is profoundly affected by its topographical structure and the interaction between the loaded molecules and nanocellulose.The applications of nanocellulose and its composites in tissue engineering have been discussed.Finally,the review is concluded with further opportunities and challenges in nanocellulose-mediated delivery of active molecules.展开更多
基金supported by the Basic Research Program through the National Research Foundation of Korea(NRF),which is financed by the Ministry of Education(Grant Nos.2018R1A6A1A03025582,2019R1D1A3A03103828,2022R1I1A3063302),Korea.
文摘Tissues are made up of cells and the extracellular matrix(ECM)which surrounds them.These cells and tissues are actively adaptable to enduring significant stress that occurs in daily life.This astonishing mechanical stress develops due to the interaction between the live cells and the non-living ECM.Cells in the matrix microenvironment can sense the signals and forces produced and initiate a signaling cascade that plays a crucial role in the body’s normal functioning and influences various properties of the native cells,including growth,proliferation,and differentiation.However,the matrix’s characteristic features also impact the repair and regeneration of the damaged tissues.The current study reviewed how the cell-ECM interaction regulates cellular behavior and physicochemical properties.Herein,we have described the response of cells to mechanical stresses,the importance of substrate stiffness and geometry in tissue regeneration,and the development of scaffolds to mimic the nature of native ECM in 3D for tissue engineering applications has also been discussed.Finally,the study summarizes the conclusions and promising prospects based on the cell-ECM interplay.
基金This research was supported by the Basic Research Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Education(NRF-2018R1A6A1A03025582)the National Research Foundation of Korea(NRF-2019R1D1A3A03103828).
文摘Mesenchymal stem cells(MSCs)are multipotent cells usually isolated from bone marrow,endometrium,adipose tissues,skin,and dental pulp.MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science.Recently,MSCs have been widely explored for their application in regenerative medicine and COVID-19 treatment.Different approaches to evaluate the future of biomaterials and stem cell properties have been developed.However,misconceptions and ethical issues still exist,such as MSCs being non-angiogenic,anti-apoptotic,and immunoregulatory competencies.Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs.These issues generate questions related to ethical and moral issues.However,MSCs have gained considerable attention for tissue regeneration owing to their differentiation ability with immunomodulatory effects.They are capable of secreting a broad range of biomolecules such as proteins,nucleic acids,exosomes,microRNAs,and membrane vesicles,collectively known as secretomes.Secretomes are released in response to the surrounding microenvironment.In this article,we briefly address topics related to the therapeutic potential of MSCs as an advanced approach in the field of regenerative medicine and various perspectives.
基金supported by the Basic Science Research Program through the National Research Foundation of Korea(Grant No.2018R1A6A1A03025582,2022R1I1A3063302)supported by the Innovative Human Resource Development for Local Intellectualization program through the Institute of Information&Communications Technology Planning&Evaluation(IITP)grant funded by the Korea government(MSIT)(IITP-2024-RS-2023-00260267)supported by the Basic Research Program through the National Research foundation of Korea(Grant No.2021R1A6A1A03046418),Republic of Korea.
文摘Biomaterial composition and surface charge play a critical role in macrophage polarization,providing a mo-lecular cue for immunomodulation and tissue regeneration.In this study,we developed bifunctional hydrogel inks for accelerating M2 macrophage polarization and exosome(Exo)cultivation for wound healing applications.For this,we first fabricated polyamine-modified three-dimensional(3D)printable hydrogels consisting of algi-nate/gelatin/polydopamine nanospheres(AG/NSPs)to boost M2-exosome(M2-Exo)secretion.The cultivated M2-Exo were finally encapsulated into a biocompatible collagen/decellularized extracellular matrix(COL@d-ECM)bioink for studying angiogenesis and in vivo wound healing study.Our findings show that 3D-printed AGP hydrogel promoted M2 macrophage polarization by Janus kinase/signal transducer of activation(JAK/STAT),peroxisome proliferator-activated receptor(PPAR)signaling pathways and facilitated the M2-Exo secretion.Moreover,the COL@d-ECM/M2-Exo was found to be biocompatible with skin cells.Transcriptomic(RNA-Seq)and real-time PCR(qRT-PCR)study revealed that co-culture of fibroblast/keratinocyte/stem cells/endothelial cells in a 3D bioprinted COL@d-ECM/M2-Exo hydrogel upregulated the skin-associated signature biomarkers through various regulatory pathways during epidermis remodeling and downregulated the mitogen-activated protein kinase(MAPK)signaling pathway after 7 days.In a subcutaneous wound model,the 3D bioprinted COL@d-ECM/M2-Exo hydrogel displayed robust wound remodeling and hair follicle(HF)induction while reducing canonical pro-inflammatory activation after 14 days,presenting a viable therapeutic strategy for skin-related disorders.
基金supported by the Basic Science Research Program’through the‘National Research Foundation of Korea’funded by the‘Ministry of Education’(NRF-2018R1A16A1A03025582,NRF-2019R1D1A3A03103828,and NRF2022R1I1A3063302).
文摘Macrophage-assisted immunomodulation is an alternative strategy in tissue engineering, wherein the interplay between pro-inflammatory and anti-inflammatory macrophage cells and body cells determines the fate of healing or inflammation. Although several reports have demonstrated that tissue regeneration depends on spatial and temporal regulation of the biophysical or biochemical microenvironment of the biomaterial, the underlying molecular mechanism behind immunomodulation is still under consideration for developing immunomodulatory scaffolds. Currently, most fabricated immunomodulatory platforms reported in the literature show regenerative capabilities of a particular tissue, for example, endogenous tissue (e.g., bone, muscle, heart, kidney, and lungs) or exogenous tissue (e.g., skin and eye). In this review, we briefly introduced the necessity of the 3D immunomodulatory scaffolds and nanomaterials, focusing on material properties and their interaction with macrophages for general readers. This review also provides a comprehensive summary of macrophage origin and taxonomy, their diverse functions, and various signal transduction pathways during biomaterial-macrophage interaction, which is particularly helpful for material scientists and clinicians for developing next-generation immunomodulatory scaffolds. From a clinical standpoint, we briefly discussed the role of 3D biomaterial scaffolds and/or nanomaterial composites for macrophage-assisted tissue engineering with a special focus on bone and associated tissues. Finally, a summary with expert opinion is presented to address the challenges and future necessity of 3D bioprinted immunomodulatory materials for tissue engineering.
基金This research was supported by the Basic Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(NRF-2018R1A6A1A03025582)and the National Research Foundation of Korea(NRF-2019R1D1A3A03103828).
文摘Nanocellulose,a biopolymer,has received wide attention from researchers owing to its superior physicochemical properties,such as high mechanical strength,low density,biodegradability,and biocompatibility.Nanocellulose can be extracted from wide range of sources,including plants,bacteria,and algae.Depending on the extraction process and dimensions(diameter and length),they are categorized into three main types:cellulose nanocrystals(CNCs),cellulose nanofibrils(CNFs),and bacterial nanocellulose(BNC).CNCs are a highly crystalline and needle-like structure,whereas CNFs have both amorphous and crystalline regions in their network.BNC is the purest form of nanocellulose.The nanocellulose properties can be tuned by chemical functionalization,which increases its applicability in biomedical applications.This review highlights the fabrication of different surface-modified nanocellulose to deliver active molecules,such as drugs,proteins,and plasmids.Nanocellulose-mediated delivery of active molecules is profoundly affected by its topographical structure and the interaction between the loaded molecules and nanocellulose.The applications of nanocellulose and its composites in tissue engineering have been discussed.Finally,the review is concluded with further opportunities and challenges in nanocellulose-mediated delivery of active molecules.
