BACKGROUND The diagnosis of inflammatory bowel disease(IBD)involves clinical,endoscopic,and radiologic evaluation.Endoscopic procedures,particularly in pediatrics,require general anesthesia and carry potential risks.A...BACKGROUND The diagnosis of inflammatory bowel disease(IBD)involves clinical,endoscopic,and radiologic evaluation.Endoscopic procedures,particularly in pediatrics,require general anesthesia and carry potential risks.AIM To investigate whether serum biomarkers can differentiate between pediatric patients with and without IBD.Secondary objectives included identifying biomarkers that distinguish Crohn’s disease(CD)from ulcerative colitis(UC)and assessing their predictive value for progression to biologic therapy.METHODS Pediatric patients undergoing diagnostic colonoscopy at British Columbia Children’s Hospital between December 2017 and June 2022 were enrolled.Blood samples were collected at colonoscopy,and demographic clinical data,laboratory,and histopathologic evaluation were obtained.An exploratory screen of 50 biomarkers was undertaken in a subset of patients(54 IBD,41 controls)using LegendplexTM flow cytometry kits to identify candidates.A refined panel of 12 serum biomarkers was subsequently selected and a supervised learning model was developed to classify patients.RESULTS The study included 246 pediatric patients,who had a median age of 13.03 years and were 37.4%female(103 CD,52 UC,91 controls).In univariate analyses,C-X-C motif chemokine ligand 9(CXCL9)was the only biomarker significantly elevated in IBD vs controls(P<0.001).A multivariable model achieved an area under the receiver operating characteristic of 0.861 for distinguishing IBD from controls.Interleukin 8(IL-8)emerged as a key biomarker alongside CXCL9 and IL-22 in the model.The random forest model identified CXCL9 with the greatest diagnostic accuracy(area under the curve[AUC]=0.81),followed by IL8 and IL22(AUC=0.737 and 0.68,respectively).CXCL9 and IL-18 showed higher levels in CD(P=0.016),whereas CXCL1 levels predicted progression to biologic therapy within 1 year(P=0.039).However,the model did not effectively predict disease subclassification or progression to biologic therapy.CONCLUSION Serum biomarkers,particularly CXCL9,IL-8,and IL-22,can aid in the diagnosis of pediatric IBD.CXCL9 and IL18 were found to be significant predictors of CD,and CXCL1 differed between patients requiring biologic therapy vs those who did not.展开更多
Soft structural textiles,or softgoods,are used within the space industry for inflatable habitats,parachutes and decelerator systems.Evaluating the safety and structural integrity of these systems occurs through struct...Soft structural textiles,or softgoods,are used within the space industry for inflatable habitats,parachutes and decelerator systems.Evaluating the safety and structural integrity of these systems occurs through structural health monitoring systems(SHM),which integrate non-invasive/non-destructive testing methods to detect,diagnose,and locate damage.Strain/load monitoring of these systems is limited while utilizing traditional strain gauges as these gauges are typically stiff,operate at low temperatures,and fail when subjected to high strain that is a result of high loading classifying them as unsuitable for SHM of soft structural textiles.For this work,a capacitance based strain gauge(CSG)was fabricated via aerosol jet printing(AJP)using silver nanoparticle ink on a flexible polymer substrate.Printed strain gauges were then compared to a commercially available high elongation resistance-based strain gauge(HE-RSG)for their ability to monitor strained Kevlar straps having a 26.7 kN(6 klbf)load.Dynamic,static and cyclic loads were used to characterize both types of strain monitoring devices.Printed CSGs demonstrated superior performance for high elongation strain measurements when compared to commonly used HE-RSGs,and were observed to operate with a gauge factor of 5.2 when the electrode arrangement was perpendicular to the direction of strain.展开更多
基金Supported by the Lutsky Family Foundation and AdMare Bioinnovations(previously known as the Genome BC CDRD Development Fund).
文摘BACKGROUND The diagnosis of inflammatory bowel disease(IBD)involves clinical,endoscopic,and radiologic evaluation.Endoscopic procedures,particularly in pediatrics,require general anesthesia and carry potential risks.AIM To investigate whether serum biomarkers can differentiate between pediatric patients with and without IBD.Secondary objectives included identifying biomarkers that distinguish Crohn’s disease(CD)from ulcerative colitis(UC)and assessing their predictive value for progression to biologic therapy.METHODS Pediatric patients undergoing diagnostic colonoscopy at British Columbia Children’s Hospital between December 2017 and June 2022 were enrolled.Blood samples were collected at colonoscopy,and demographic clinical data,laboratory,and histopathologic evaluation were obtained.An exploratory screen of 50 biomarkers was undertaken in a subset of patients(54 IBD,41 controls)using LegendplexTM flow cytometry kits to identify candidates.A refined panel of 12 serum biomarkers was subsequently selected and a supervised learning model was developed to classify patients.RESULTS The study included 246 pediatric patients,who had a median age of 13.03 years and were 37.4%female(103 CD,52 UC,91 controls).In univariate analyses,C-X-C motif chemokine ligand 9(CXCL9)was the only biomarker significantly elevated in IBD vs controls(P<0.001).A multivariable model achieved an area under the receiver operating characteristic of 0.861 for distinguishing IBD from controls.Interleukin 8(IL-8)emerged as a key biomarker alongside CXCL9 and IL-22 in the model.The random forest model identified CXCL9 with the greatest diagnostic accuracy(area under the curve[AUC]=0.81),followed by IL8 and IL22(AUC=0.737 and 0.68,respectively).CXCL9 and IL-18 showed higher levels in CD(P=0.016),whereas CXCL1 levels predicted progression to biologic therapy within 1 year(P=0.039).However,the model did not effectively predict disease subclassification or progression to biologic therapy.CONCLUSION Serum biomarkers,particularly CXCL9,IL-8,and IL-22,can aid in the diagnosis of pediatric IBD.CXCL9 and IL18 were found to be significant predictors of CD,and CXCL1 differed between patients requiring biologic therapy vs those who did not.
基金This material is based upon work supported under an Integrated University Program Graduate Fellowship,and was supported in part by Department of Energy In-Pile Instrumentation program under DOE Idaho Operations Office Contract DE-AC07-05ID14517 and by the National Aeronautics Space Administration under award#80NSSC18M0088The views and opinions of authors expressed herein do not necessarily state or reflect those of the U.S.Government or any agency thereof.D.E.also acknowledges career development support by Institutional Development Awards(IDeA)from the National Institute of General Medical Sciences of the National Institutes of Health under Grants#P20GM103408 and P20GM109095.
文摘Soft structural textiles,or softgoods,are used within the space industry for inflatable habitats,parachutes and decelerator systems.Evaluating the safety and structural integrity of these systems occurs through structural health monitoring systems(SHM),which integrate non-invasive/non-destructive testing methods to detect,diagnose,and locate damage.Strain/load monitoring of these systems is limited while utilizing traditional strain gauges as these gauges are typically stiff,operate at low temperatures,and fail when subjected to high strain that is a result of high loading classifying them as unsuitable for SHM of soft structural textiles.For this work,a capacitance based strain gauge(CSG)was fabricated via aerosol jet printing(AJP)using silver nanoparticle ink on a flexible polymer substrate.Printed strain gauges were then compared to a commercially available high elongation resistance-based strain gauge(HE-RSG)for their ability to monitor strained Kevlar straps having a 26.7 kN(6 klbf)load.Dynamic,static and cyclic loads were used to characterize both types of strain monitoring devices.Printed CSGs demonstrated superior performance for high elongation strain measurements when compared to commonly used HE-RSGs,and were observed to operate with a gauge factor of 5.2 when the electrode arrangement was perpendicular to the direction of strain.
