Carnitine(CAR) is a vital quaternary ammonium compound. However, the characteristics of CAR content and its influencing factors across different marine animal species remain poorly understood. This study examined the ...Carnitine(CAR) is a vital quaternary ammonium compound. However, the characteristics of CAR content and its influencing factors across different marine animal species remain poorly understood. This study examined the CAR content in 258 marine animals, representing 54 species across seven classes, collected from coastal and offshore regions of China and the Northwest Pacific Ocean(NWPO) between May 2020 and June 2022. The CAR content ranged from0.01 mmol/kg to 1.33 mmol/kg, with a mean value of(0.15 ± 0.18) mmol/kg(mean ± standard deviation). The highest CAR content was observed in Crustacea [(0.48 ± 0.38) mmol/kg], which was four times greater than the average CAR content in the other classes [(0.12 ± 0.11) mmol/kg]. Overall, the CAR content exhibited minor variation among marine animals, potentially because the carnitine pool maintains its dynamic balance. Additionally, significant correlations were observed between the CAR content and environmental factors in some species: a negative correlation with seawater temperature and positive correlations with latitude, seawater salinity, and depth. These results suggest that the CAR content in marine animals is influenced by environmental changes. This study provides theoretical insights for understanding the characteristics and influencing factors of CAR content in marine animals.展开更多
To characterize environmental factors controlling decadal-scale variations in the buried flux of marine organic carbon(BFC_(m))in the eastern shelf sea areas of China(ECSS),four well preserved sediment cores collected...To characterize environmental factors controlling decadal-scale variations in the buried flux of marine organic carbon(BFC_(m))in the eastern shelf sea areas of China(ECSS),four well preserved sediment cores collected from the central Yellow Sea mud(CYSM)area,the Yellow Sea Coastal Current(YSCC)area and the Changjiang River Estuary(CRE)were investigated in this study.In the CYSM,variations in BFC_(m) were found to be dependent on variations in primary productivity and to exhibit a cyclical trend possibly related to fluctuations in the Pacific Decadal Oscillation(PDO)and the East Asian winter monsoon index(EAWM).In the YSCC,BFC_(m) likewise depends on primary productivity.Prior to the 1950s,variations in BFC_(m) were similar to that of the EAWM.After the 1950s,BFC_(m) increased rapidly and exhibited maximum values in the surface layer,consistent with an increase in primary productivity caused by the input of terrestrial nutrients associated with China’s economic development.In the CRE,variations in BFC_(m) were affected by several competing factors making it difficult to identify clear relationships between variations in BFC_(m) and primary productivity.In contrast,long-term variability in BFC_(m) is more similar to changes in the Changjiang River sediment load.Thus,it is speculated that the construction of dams along the Changjiang River may be the main cause of variations in BFC_(m) in this area.Given the disproportionate effects of human activities on marine environments and decadal variations in BFC_(m) in the ECSS,careful attention should be paid to regional differences in organic carbon preservation and environmental changes lest estimates of these values be made imprecise or inaccurate.展开更多
High-throughput measurements of ciprofloxacin, clomipramine and fexofenadine hydrochlorides were performed by employing an automatic 8-channel electrical titrator. Silver nitrate （AgNO3） and sodium tetraphenylborate...High-throughput measurements of ciprofloxacin, clomipramine and fexofenadine hydrochlorides were performed by employing an automatic 8-channel electrical titrator. Silver nitrate （AgNO3） and sodium tetraphenylborate （NaTPB） were used as titrants. When AgNO3 was used for measuring the drugs in pure form, recoveries were 97.6%-102.0% with RSD values ≤1.0%; for measuring them in pharmaceutical formulations, recoveries were 96.6%-99.1% with RSD values ≤1.0%. Batch samples of eight could be measured simultaneously and maximally 30 measurements per minute could be completed. When NaTPB was used for measuring the drugs in pure form, the recoveries were 96.8%-102.6% with RSD values 〈0.8%; for measuring them in pharmaceutical formulations, the recoveries were 97.5%-102.7% with RSD values ≤0.9%. For all analyses, no auxiliary devices or chemicals were needed and there was no requirement for changing or cleaning working electrodes between measurements. The efficiency, accuracy and precision of the proposed method make it an alternative for routine quality control analyses.展开更多
基金The Shandong Provincial Natural Science Foundation of China under contract No.ZR2022MD055Youth Program of National Natural Science Foundation of China under contract No. 41606097Central Public interest Scientific Institution Basal Research Fund of CAFS under contract No. 2023TD53。
文摘Carnitine(CAR) is a vital quaternary ammonium compound. However, the characteristics of CAR content and its influencing factors across different marine animal species remain poorly understood. This study examined the CAR content in 258 marine animals, representing 54 species across seven classes, collected from coastal and offshore regions of China and the Northwest Pacific Ocean(NWPO) between May 2020 and June 2022. The CAR content ranged from0.01 mmol/kg to 1.33 mmol/kg, with a mean value of(0.15 ± 0.18) mmol/kg(mean ± standard deviation). The highest CAR content was observed in Crustacea [(0.48 ± 0.38) mmol/kg], which was four times greater than the average CAR content in the other classes [(0.12 ± 0.11) mmol/kg]. Overall, the CAR content exhibited minor variation among marine animals, potentially because the carnitine pool maintains its dynamic balance. Additionally, significant correlations were observed between the CAR content and environmental factors in some species: a negative correlation with seawater temperature and positive correlations with latitude, seawater salinity, and depth. These results suggest that the CAR content in marine animals is influenced by environmental changes. This study provides theoretical insights for understanding the characteristics and influencing factors of CAR content in marine animals.
基金The Fund of Key Laboratory of Control of Quality and Safety for Aquatic Products,Ministry of Agriculture and Rural Affairs,P.R.China under contract No.2021C001the National Key Research and Development Program of China under contract No.2018YFD0900703the Major Scientific and Technological Innovation Project of Shandong Provincial Key Research and Development Program under contract No.2019JZZY020706。
文摘To characterize environmental factors controlling decadal-scale variations in the buried flux of marine organic carbon(BFC_(m))in the eastern shelf sea areas of China(ECSS),four well preserved sediment cores collected from the central Yellow Sea mud(CYSM)area,the Yellow Sea Coastal Current(YSCC)area and the Changjiang River Estuary(CRE)were investigated in this study.In the CYSM,variations in BFC_(m) were found to be dependent on variations in primary productivity and to exhibit a cyclical trend possibly related to fluctuations in the Pacific Decadal Oscillation(PDO)and the East Asian winter monsoon index(EAWM).In the YSCC,BFC_(m) likewise depends on primary productivity.Prior to the 1950s,variations in BFC_(m) were similar to that of the EAWM.After the 1950s,BFC_(m) increased rapidly and exhibited maximum values in the surface layer,consistent with an increase in primary productivity caused by the input of terrestrial nutrients associated with China’s economic development.In the CRE,variations in BFC_(m) were affected by several competing factors making it difficult to identify clear relationships between variations in BFC_(m) and primary productivity.In contrast,long-term variability in BFC_(m) is more similar to changes in the Changjiang River sediment load.Thus,it is speculated that the construction of dams along the Changjiang River may be the main cause of variations in BFC_(m) in this area.Given the disproportionate effects of human activities on marine environments and decadal variations in BFC_(m) in the ECSS,careful attention should be paid to regional differences in organic carbon preservation and environmental changes lest estimates of these values be made imprecise or inaccurate.
基金supported by Special Scientific Research Funds for Central Non-profit Institutes,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences(YSFRI-CAFS)(No.20603022016003)Import of International Advanced Agricultural Science and Technology Plan(948 Project)of Chinese Ministry of Agriculture(No.2016-X28)+2 种基金Central Public Interest Scientific Institution Basal Research Fund,CAFS(No.2016RC-BR02)Qingdao National Laboratory for Marine Science and Technology(No.2015ASKJ02-05)Key R&D Program of Shandong Province(No.2016GSF120008)
文摘High-throughput measurements of ciprofloxacin, clomipramine and fexofenadine hydrochlorides were performed by employing an automatic 8-channel electrical titrator. Silver nitrate （AgNO3） and sodium tetraphenylborate （NaTPB） were used as titrants. When AgNO3 was used for measuring the drugs in pure form, recoveries were 97.6%-102.0% with RSD values ≤1.0%; for measuring them in pharmaceutical formulations, recoveries were 96.6%-99.1% with RSD values ≤1.0%. Batch samples of eight could be measured simultaneously and maximally 30 measurements per minute could be completed. When NaTPB was used for measuring the drugs in pure form, the recoveries were 96.8%-102.6% with RSD values 〈0.8%; for measuring them in pharmaceutical formulations, the recoveries were 97.5%-102.7% with RSD values ≤0.9%. For all analyses, no auxiliary devices or chemicals were needed and there was no requirement for changing or cleaning working electrodes between measurements. The efficiency, accuracy and precision of the proposed method make it an alternative for routine quality control analyses.
