Objective:This study aims to investigate the potential molecular mechanism of Salvia miltiorrhiza in treating benign prostatic hyperplasia(BPH)based on network pharmacology.Methods:Active components of Salvia miltiorr...Objective:This study aims to investigate the potential molecular mechanism of Salvia miltiorrhiza in treating benign prostatic hyperplasia(BPH)based on network pharmacology.Methods:Active components of Salvia miltiorrhiza were screened via the TCMSP database,and their potential targets were predicted using Swiss Target Prediction.BPH-related targets were obtained from Gene Cards and OMIM databases.Common targets between the herb and BPH were used to con-struct a protein-protein interaction(PPI)network via STRING and visualized using Cytoscape.Core targets were identifi ed,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were conducted(P≤0.01).Results:A total of 57 active components and 818 targets of Salvia miltiorrhiza were identifi ed.Intersection analysis yielded 458 potential targets associated with BPH.PPI network analysis revealed core targets including SRC,PIK3R1,and PIK3CA.GO enrichment analysis indicated that the targets were primarily associated with biological processes(BP)such as calcium ion homeostasis,cellular components(CC)including focal adhesions,and molecular functions(MF)such as tyrosine kinase activity.KEGG pathway analysis indicated that Salvia miltiorrhiza may exert therapeutic effects through pathways including MAPK,PI3K-Akt,and calcium signaling(P≤0.01).Conclusion:Salvia miltiorrhiza may regulate BPH through a multi-component,multi-target,and multi-pathway network,providing a theoretical basis for its clinical application.展开更多
Thin-walled torispherical heads under internal pressure can fail by plastic buckling because of compressive circumferential stresses in the head knuckle.However,existing formulas still have limitations,such as complic...Thin-walled torispherical heads under internal pressure can fail by plastic buckling because of compressive circumferential stresses in the head knuckle.However,existing formulas still have limitations,such as complicated expressions and low accuracy,in determining buckling pressure.In this paper,we propose a new formula for calculating the buckling pressure of torispherical heads based on elastic-plastic analysis and experimental results.First,a finite element(FE)method based on the arc-length method is established to calculate the plastic buckling pressure of torispherical heads,considering the effects of material strain hardening and geometrical nonlinearity.The buckling pressure results calculated by the FE method in this paper have good consistency with those of BOSOR5,which is a program for calculating the elastic-plastic bifurcation buckling pressure based on the finite difference energy method.Second,the effects of geometric parameters,material parameters,and restraint form of head edge on buckling pressure are investigated.Third,a new formula for calculating plastic buckling pressure is developed by fitting the curve of FE results and introducing a reduction factor determined from experimental data.Finally,based on the experimental results,we compare the predictions of the new formula with those of existing formulas.It is shown that the new formula has a higher accuracy than the existing ones.展开更多
Translucency is a recurring problem for pineapple industry. Translucent fruit contained more sucrose, glucose and fructose in apoplast than those in apoplast of normal fruit. There were more liquid in intercellular sp...Translucency is a recurring problem for pineapple industry. Translucent fruit contained more sucrose, glucose and fructose in apoplast than those in apoplast of normal fruit. There were more liquid in intercellular space of translucent fruit than that of normal flesh. The contents of alcohol and ethylene in translucent fruit were higher than those in normal fruit. Translucent fruit contained less calcium than normal fruit. Electrolyte leakage of translucent flesh was more than that of normal flesh. There were 205 proteins of which the expressions in translucent flesh were higher than those in normal flesh. Calcium-ions-binding protein EF-hand domain-containing protein, ethylene-synthesizing enzyme 1-aminpcyclopropane-1-carboxylate oxidase, ROS-producing protein universal stress protein A-like protein were the top three proteins of which the expressions in translucent flesh were higher than those in normal fruit. When much sugar was transferred into fruit pulp and accumulated in intercellular space, water will be absorbed from cells around and translucence formed. The accumulation of sugar and liquid in apoplast were due to that cell wall and membrane were degraded, which was from being attacked by ROS. There might be more and larger pores in cell wall and membranes of translucent flesh. These data played foundations for researching methods for controlling pineapple translucency.展开更多
Pineapple plant was always injured due to low temperature. Breeding varieties with high cold tolerance was the supreme way to resolve this problem and identifying cold tolerance of germplasms collected was necessary. ...Pineapple plant was always injured due to low temperature. Breeding varieties with high cold tolerance was the supreme way to resolve this problem and identifying cold tolerance of germplasms collected was necessary. In this study, SSR locus around homologous sequences of cold-tolerant genes w</span><span style="font-family:"">as</span><span style="font-family:""> searched and screened using MD-2 (cold-tolerant variety) and Tainong 17 (cold-sensitive variety). Seventy three pairs of primers of which PCR results were different between these two varieties were gotten. PCRs were performed using these as primers and genomic DNA of germplasms collected as templates. Six pairs of primers were found that their PCR results were in good consistent with cold tolerance. Using GLM (General linear model) association <span>mapping analysis, two SSR markers linked to cold tolerance were found. Their</span> efficiency was verified further using pineapple germplasms with high cold tolerance which had been validated in field by cold wave. They will be used in cold-tolerant breeding of pineapple in the future.展开更多
Objective:Tanshinone ⅡA,one of the most abundant liposoluble components isolated from the traditional Chinese medicine Salvia miltiorrhiza,exhibits significant biological activities in anti-inflammatory,antibacterial...Objective:Tanshinone ⅡA,one of the most abundant liposoluble components isolated from the traditional Chinese medicine Salvia miltiorrhiza,exhibits significant biological activities in anti-inflammatory,antibacterial,and antitumor eff ects.This study aims to systematically explore the mechanism of Tanshinone ⅡA through bioinformatics.Methods:We utilized the TCMSP database to retrieve the oral bioavailability(OB)and drug-likeness(DL)of Tanshinone ⅡA.The gene chip numbered GSE85871 was downloaded from the GEO database,and diff erential genes were analyzed using R language to identify potential targets of Tanshinone ⅡA.After obtaining these targets,GO analysis and KEGG pathway analysis were performed using the DAVID 6.8 database.Diseases related to Tanshinone ⅡA were explored through the CTD database.Finally,Cytoscape was employed to construct a visual network of multiple targets,pathways,and diseases associated with Tanshinone ⅡA.Results:Tanshinone ⅡA demonstrated good drug effi cacy with an OB value of 49.89%and a DL value of 0.4.A total of 132 potential targets were identifi ed,primarily exhibiting gene co-expression and physical interaction in the PPI network.These targets were enriched in biological processes and pathways such as ovarian steroidogenesis,cell cycle,and steroid hormone biosynthesis.Tanshinone ⅡA was found to be relevant in the treatment of diseases including breast tumors,hypertension,atherosclerosis,gliomas,vascular system injuries,left ventricular hypertrophy,leukemia,and hearing loss.Conclusion:Utilizing bioinformatics approaches,we systematically analyzed the possible molecular mechanisms of Tanshinone ⅡA,providing potential targets and insights into its pharmacological mechanisms and treatment strategies.展开更多
Forcing flower is an important step for planting pineapple. Although many papers have been reported about forcing flower for pineapple plants on the aim of harvesting fruits, no reports have been found about forcing f...Forcing flower is an important step for planting pineapple. Although many papers have been reported about forcing flower for pineapple plants on the aim of harvesting fruits, no reports have been found about forcing flower at seedling phase for preparing bonsai. In this paper, Tainong 4, Tainong 16 and Tainong 17 plants with 20 leaves were watered on stem apex with 40 ml of 0.13% of ethephron. Results showed that most of Tainong 4 plants flowered after being treated 30 days. None plant of Tainong 16 flowered after being treated 60 days. In the same variety, for example, Tainong 4, some plants flowered earlier, some plants flowered late. Tainong 4 was the most sensitive to ethephron at early-growth phase. Tainong 16 was the most insensitive variety. The low flower rate in this research might be due to that the pineapple plants were in early-vegetative-growth stage. They had not appropriate physiological conditions for forcing flowers. For making pineapple bonsai at early-vegetative-growth phase, higher doses of forcing-flower agent should be used than those of forcing-flower agent for harvesting fruits.展开更多
Pineapple is nutrient and planted in many tropical and subtropical countries. Plenty of elite seedlings were always needed in short time. The common methods for propagating pineapple seedlings have low-multiplying ind...Pineapple is nutrient and planted in many tropical and subtropical countries. Plenty of elite seedlings were always needed in short time. The common methods for propagating pineapple seedlings have low-multiplying index or take long time. A new method was introduced in this paper. Shoots were induced using stem sections. And then, the shoot bases were cut and cultured on callus-induced medium. The meristematic granules induced were cultured on shoot-induced medium. Shoots can be induced from the meristematic granules. Seedlings can be induced from these shoots in short time. This method overcomes the defects of the common ways. Enough seedlings can be gotten in short time. The operation is simple and easy to learn.展开更多
Background: The medical community has been trying to reduce the high mortality rate of cardiovascular diseases and find effective treatments;however, the results have not been satisfactory. Zhuangtongyin (ZTY), a Chin...Background: The medical community has been trying to reduce the high mortality rate of cardiovascular diseases and find effective treatments;however, the results have not been satisfactory. Zhuangtongyin (ZTY), a Chinese herbal formula, has received significant attention owing to its efficacy in the treatment of myocardial ischemia. However, there are few reports on the mechanisms of its action, which hinder its widespread dissemination. Recent studies have shown that the effects of ZTY are associated with the NF-κB/NLRP3 inflammasome pathway. In this study, we investigated the underlying mechanisms of ZTY in myocardial ischemia through the NF-κB/NLRP3 inflammasome pathway. Methods: A myocardial ischemia model was established by ligating rats’ left anterior descending coronary artery. The rats were randomly divided into five groups: control group, model group, sham operation group, ZTY group (13.6 g/kg), and NF-κB group (0.12 g/kg). All groups, except the control group, received oral administration for 28 days. The NF-κB group received peritoneal injection of PDTC for 28 days. ECG, assessment of myocardial ischemia injury, histological examination, and analysis of biochemical indices of myocardial tissue were performed to evaluate myocardial injury and drug protection. Inflammation levels were assessed by Masson staining and Elisa, and expression of transforming growth factor (TGF-β1), α-smooth muscle actin (α-SMA), and connexin 43 (Cx43) was detected by immunohistochemistry. Results: Compared to the control group, the model group showed obvious abnormalities in myocardial tissue and increased levels of inflammatory cytokines. ZTY therapy inhibited the increases in lactate dehydrogenase (LDH), creatinine kinase (CK), creatinine kinase isoenzyme (CK-MB), tumour necrosis factor α (TNF-α), and interleukin-6 (IL-6). The immunohistochemical results showed that ZTY treatment inhibited TGF-β1 expression, suggesting that the anti-inflammatory effect of ZTY was achieved by inhibiting NF-κB signalling. Conclusion: ZTY can effectively alleviate myocardial injury, and its protective effect against myocardial injury is related to inhibition of the NF-κB/NLRP3 inflammasome pathway.展开更多
Pineapple industry has played important roles in China’s economics in tropical- and subtropical-areas. Pineapple industry in China has been damaged by water-heart fruit. The objective of this research is to clarify t...Pineapple industry has played important roles in China’s economics in tropical- and subtropical-areas. Pineapple industry in China has been damaged by water-heart fruit. The objective of this research is to clarify the mechanism underlying water-heart fruit of pineapple and find ways for preventing water-heart fruit. We found that water logging can cause water-heart pineapple. The longer the pineapple plants were water logged, the more fruits were found water heart. With the development of fruits, the fruits’ sensitivity to water logging increased. Spraying potassium sulfate onto the fruits after the plants are water logged can decrease the rate of pineapple water-heart. But this effect became less with the fruit development. When sucrose and its precursors in intracellular space were transferred into sink cells, hydrogen ions were required. Because of lacking hydrogen ions, sucrose and its precursors were stopped in intracellular space and seized water from cells. Water-heart fruit formed. If potassium can be gotten in intracellular space, the roles of hydrogen ions might be replaced with potassium ions. With the help of potassium ions, sucrose and its precursors were transported into sink cells and the water-heart symptoms alleviated.展开更多
This paper studies the effect of option trading on corporate investment and financing policies.Based on prior literature,I hypothesize that option market induces informed trading and thus reduces information asymmetry...This paper studies the effect of option trading on corporate investment and financing policies.Based on prior literature,I hypothesize that option market induces informed trading and thus reduces information asymmetry and the cost of capital.As a result,firms with high option trading have more investment and financing.Specifically,based on the United States public data,this paper finds that option trading volume increases corporate investment and financing,but reduces cash holdings and corporate payouts.These results are robust to the inclusion of industry or firm fixed effect,a control for endogenous options trading,and the use of alternative measures of option trading and corporate policies.The effect of option trading is stronger for firms with higher information asymmetry problems.Finally,this paper finds the results are inconsistent with the“quiet Life”hypothesis and the catering hypothesis.展开更多
It is critical to combat tumor metastasis by eradicating disseminated tumor cells in any step during the metastasis process.After entering the blood circulation system,tumor cells are in suspension and experience cons...It is critical to combat tumor metastasis by eradicating disseminated tumor cells in any step during the metastasis process.After entering the blood circulation system,tumor cells are in suspension and experience considerable levels of fluid shear stress.However,the influence of hemodynamic shear stress on the survival of CTCs and the underlying mechanotransduction mechanisms remain unclear.This study shows that fluid shear stress can eliminate the majority of CTCs and the viability of suspended tumor cells depends on the stress magnitude,indicating that tumor cells can sense and respond to fluid shear stress.Mechanistically,the expression of Piezo1 but not Piezo2 is greatly upregulated in suspended tumor cells after shear stress treatment.Inhibiting/activating Piezo1 increases/decreases the viability of suspended tumor cells in shear flow,which depends on Piezo1-mediated calcium entry.These findings suggest that Piezo1 may be the major mechanosensor by which suspended tumor cells sense fluid shear stress.As the downstream effector of Piezo1,actomyosin in tumor cells is significantly activated under increasing shear stress.Its activity influences the survival of CTCs in shear flow and rescues the effects of Piezo1 on tumor cell survival,suggesting that hemodynamic shear stress regulates the survival of CTCs through Piezo1 mediated actomyosin activity.Importantly,fluid shear stress considerably up-regulates YAP/TAZ activity of suspended tumor cells and promotes their nuclear translocation in a magnitude-dependent manner.Inhibiting YAP/TAZ enhances the viability of suspended tumor cells in shear stress,while activating their activity decreases tumor cell survival,suggesting that YAP/TAZ activation promotes the apoptosis of suspended tumor cells,which is different from the findings that YAP/TAZ facilitates the survival of adherent cells to shear flow.Further,blocking the nuclear import of YAP/TAZ inactivates the sensation of suspended tumor cells to fluid shear flow and attenuates the dependence of tumor cell survival on different magnitudes of hemodynamic shear stress.The influence of Piezo1-actomyosin pathway on suspended tumor cells can be rescued by YAP/TAZ activity,suggesting that Piezo1-mediated signaling induces tumor cell apoptosis via nuclear translocation of YAP/TAZ.In addition,fluid shear stress can also activate the expressions of LATS1/2 and MST1/2 in Hippo pathway through Piezo1,which is known to inhibit YAP/TAZ activity.Silencing/activating LATS1/2 or MST1/2 inhibits/enhances the viability of CTCs under shear stress,the effects of which can be further rescued by YAP/TAZ.These findings suggest that the responses of suspended tumor cells to hemodynamic shear stress are partially mediated by Hippo signaling.After nuclear translocation,YAP/TAZ directly bind p73/PUMA,which further promotes the transcription of pro-apoptotic genes and induces the apoptosis of suspended tumor cells.In summary,these findings show that hemodynamic shear stress considerably influences the survival of CTCs in blood circulation.We have identified the calcium channel Piezo1 as a novel mechanosensor for the response of CTCs to fluid shear stress.Hemodynamic shear stress induces the apoptosis of suspended tumor cells through Piezo1-actomyosin-YAP/TAZ-p73/PUMA signaling,which is different from the mechanotranduction mechanisms for tumor cells in adherent.Therefore,this study has unveiled the novel mechanosensor of suspended CTCs in response to fluid shear stress and the subsequent mechanisms and identified Piezo1 and YAP/TAZ as the potential therapeutic targets,through which CTCs may be effectively eradicated in the vasculature to prohibit tumor metastasis.展开更多
Tumor cells progressively remodel cytoskeletal structures and reduce cellular stiffness during tumor progression,implicating the correlation between cell mechanics and malignancy.However,the roles of tumor cell cytosk...Tumor cells progressively remodel cytoskeletal structures and reduce cellular stiffness during tumor progression,implicating the correlation between cell mechanics and malignancy.However,the roles of tumor cell cytoskeleton and the mechanics in tumor progression remain incompletely understood.We report that softening/stiffening tumor cells by targeting actomyosin promotes/suppresses self-renewal in vitro and tumorigenic potential in vivo.Weakening/strengthening actin cytoskeleton impairs/reinforces the interaction between adenomatous polyposis coli(APC)andβ-catenin,which facilitatesβ-catenin nuclear/cytoplasmic localization.Nuclearβ-catenin binds to the promoter of Oct4,which enhances its transcription that is crucial in sustaining self-renewal and malignancy.These results demonstrate that the mechanics of tumor cells dictate self-renewal through cytoskeleton-APC-Wnt/β-catenin-Oct4 signaling,which are correlated with tumor differentiation and patient survival.This study unveils an uncovered regulatory role of cell mechanics in self-renewal and malignancy,and identifies tumor cell mechanics as a hallmark not only for cancer diagnosis but also for mechanotargeting.展开更多
The use of conjugated microporous polymers(CMPs)in photocatalytic CO_(2)reduction(CO_(2)RR),leveraging solar energy and water to generate carbon-based products,is attracting considerable attention.However,the amorphou...The use of conjugated microporous polymers(CMPs)in photocatalytic CO_(2)reduction(CO_(2)RR),leveraging solar energy and water to generate carbon-based products,is attracting considerable attention.However,the amorphous nature of most CMPs poses challenges for effective charge carrier separation,limiting their application in CO_(2)RR.In this study,we introduce an innovative approach utilizing donorπ-skeleton engineering to enhance skeleton coplanarity,thereby achieving highly crystalline CMPs.Advanced femtosecond transient absorption and temperature-dependent photoluminescence analyses reveal efficient exciton dissociation into free charge carriers that actively engage in surface reactions.Complementary theoretical calculations demonstrate that our highly crystalline CMP(Py-TDO)not only greatly improves the separation and transfer of photoexcited charge carriers but also introduces additional charge transport pathways via intermolecularπ-πstacking.Py-TDO exhibits outstanding photocatalytic CO_(2) reduction capabilities,achieving a remarkable CO generation rate of 223.97μmol g^(-1)h^(-1)without the addition of chemical scavengers.This work lays pioneering groundwork for the development of novel highly crystalline materials,advancing the field of solar-driven energy conversion.展开更多
文摘Objective:This study aims to investigate the potential molecular mechanism of Salvia miltiorrhiza in treating benign prostatic hyperplasia(BPH)based on network pharmacology.Methods:Active components of Salvia miltiorrhiza were screened via the TCMSP database,and their potential targets were predicted using Swiss Target Prediction.BPH-related targets were obtained from Gene Cards and OMIM databases.Common targets between the herb and BPH were used to con-struct a protein-protein interaction(PPI)network via STRING and visualized using Cytoscape.Core targets were identifi ed,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were conducted(P≤0.01).Results:A total of 57 active components and 818 targets of Salvia miltiorrhiza were identifi ed.Intersection analysis yielded 458 potential targets associated with BPH.PPI network analysis revealed core targets including SRC,PIK3R1,and PIK3CA.GO enrichment analysis indicated that the targets were primarily associated with biological processes(BP)such as calcium ion homeostasis,cellular components(CC)including focal adhesions,and molecular functions(MF)such as tyrosine kinase activity.KEGG pathway analysis indicated that Salvia miltiorrhiza may exert therapeutic effects through pathways including MAPK,PI3K-Akt,and calcium signaling(P≤0.01).Conclusion:Salvia miltiorrhiza may regulate BPH through a multi-component,multi-target,and multi-pathway network,providing a theoretical basis for its clinical application.
基金supported by the National Natural Science Foundation of China(No.52105161).
文摘Thin-walled torispherical heads under internal pressure can fail by plastic buckling because of compressive circumferential stresses in the head knuckle.However,existing formulas still have limitations,such as complicated expressions and low accuracy,in determining buckling pressure.In this paper,we propose a new formula for calculating the buckling pressure of torispherical heads based on elastic-plastic analysis and experimental results.First,a finite element(FE)method based on the arc-length method is established to calculate the plastic buckling pressure of torispherical heads,considering the effects of material strain hardening and geometrical nonlinearity.The buckling pressure results calculated by the FE method in this paper have good consistency with those of BOSOR5,which is a program for calculating the elastic-plastic bifurcation buckling pressure based on the finite difference energy method.Second,the effects of geometric parameters,material parameters,and restraint form of head edge on buckling pressure are investigated.Third,a new formula for calculating plastic buckling pressure is developed by fitting the curve of FE results and introducing a reduction factor determined from experimental data.Finally,based on the experimental results,we compare the predictions of the new formula with those of existing formulas.It is shown that the new formula has a higher accuracy than the existing ones.
文摘Translucency is a recurring problem for pineapple industry. Translucent fruit contained more sucrose, glucose and fructose in apoplast than those in apoplast of normal fruit. There were more liquid in intercellular space of translucent fruit than that of normal flesh. The contents of alcohol and ethylene in translucent fruit were higher than those in normal fruit. Translucent fruit contained less calcium than normal fruit. Electrolyte leakage of translucent flesh was more than that of normal flesh. There were 205 proteins of which the expressions in translucent flesh were higher than those in normal flesh. Calcium-ions-binding protein EF-hand domain-containing protein, ethylene-synthesizing enzyme 1-aminpcyclopropane-1-carboxylate oxidase, ROS-producing protein universal stress protein A-like protein were the top three proteins of which the expressions in translucent flesh were higher than those in normal fruit. When much sugar was transferred into fruit pulp and accumulated in intercellular space, water will be absorbed from cells around and translucence formed. The accumulation of sugar and liquid in apoplast were due to that cell wall and membrane were degraded, which was from being attacked by ROS. There might be more and larger pores in cell wall and membranes of translucent flesh. These data played foundations for researching methods for controlling pineapple translucency.
文摘Pineapple plant was always injured due to low temperature. Breeding varieties with high cold tolerance was the supreme way to resolve this problem and identifying cold tolerance of germplasms collected was necessary. In this study, SSR locus around homologous sequences of cold-tolerant genes w</span><span style="font-family:"">as</span><span style="font-family:""> searched and screened using MD-2 (cold-tolerant variety) and Tainong 17 (cold-sensitive variety). Seventy three pairs of primers of which PCR results were different between these two varieties were gotten. PCRs were performed using these as primers and genomic DNA of germplasms collected as templates. Six pairs of primers were found that their PCR results were in good consistent with cold tolerance. Using GLM (General linear model) association <span>mapping analysis, two SSR markers linked to cold tolerance were found. Their</span> efficiency was verified further using pineapple germplasms with high cold tolerance which had been validated in field by cold wave. They will be used in cold-tolerant breeding of pineapple in the future.
文摘Objective:Tanshinone ⅡA,one of the most abundant liposoluble components isolated from the traditional Chinese medicine Salvia miltiorrhiza,exhibits significant biological activities in anti-inflammatory,antibacterial,and antitumor eff ects.This study aims to systematically explore the mechanism of Tanshinone ⅡA through bioinformatics.Methods:We utilized the TCMSP database to retrieve the oral bioavailability(OB)and drug-likeness(DL)of Tanshinone ⅡA.The gene chip numbered GSE85871 was downloaded from the GEO database,and diff erential genes were analyzed using R language to identify potential targets of Tanshinone ⅡA.After obtaining these targets,GO analysis and KEGG pathway analysis were performed using the DAVID 6.8 database.Diseases related to Tanshinone ⅡA were explored through the CTD database.Finally,Cytoscape was employed to construct a visual network of multiple targets,pathways,and diseases associated with Tanshinone ⅡA.Results:Tanshinone ⅡA demonstrated good drug effi cacy with an OB value of 49.89%and a DL value of 0.4.A total of 132 potential targets were identifi ed,primarily exhibiting gene co-expression and physical interaction in the PPI network.These targets were enriched in biological processes and pathways such as ovarian steroidogenesis,cell cycle,and steroid hormone biosynthesis.Tanshinone ⅡA was found to be relevant in the treatment of diseases including breast tumors,hypertension,atherosclerosis,gliomas,vascular system injuries,left ventricular hypertrophy,leukemia,and hearing loss.Conclusion:Utilizing bioinformatics approaches,we systematically analyzed the possible molecular mechanisms of Tanshinone ⅡA,providing potential targets and insights into its pharmacological mechanisms and treatment strategies.
文摘Forcing flower is an important step for planting pineapple. Although many papers have been reported about forcing flower for pineapple plants on the aim of harvesting fruits, no reports have been found about forcing flower at seedling phase for preparing bonsai. In this paper, Tainong 4, Tainong 16 and Tainong 17 plants with 20 leaves were watered on stem apex with 40 ml of 0.13% of ethephron. Results showed that most of Tainong 4 plants flowered after being treated 30 days. None plant of Tainong 16 flowered after being treated 60 days. In the same variety, for example, Tainong 4, some plants flowered earlier, some plants flowered late. Tainong 4 was the most sensitive to ethephron at early-growth phase. Tainong 16 was the most insensitive variety. The low flower rate in this research might be due to that the pineapple plants were in early-vegetative-growth stage. They had not appropriate physiological conditions for forcing flowers. For making pineapple bonsai at early-vegetative-growth phase, higher doses of forcing-flower agent should be used than those of forcing-flower agent for harvesting fruits.
文摘Pineapple is nutrient and planted in many tropical and subtropical countries. Plenty of elite seedlings were always needed in short time. The common methods for propagating pineapple seedlings have low-multiplying index or take long time. A new method was introduced in this paper. Shoots were induced using stem sections. And then, the shoot bases were cut and cultured on callus-induced medium. The meristematic granules induced were cultured on shoot-induced medium. Shoots can be induced from the meristematic granules. Seedlings can be induced from these shoots in short time. This method overcomes the defects of the common ways. Enough seedlings can be gotten in short time. The operation is simple and easy to learn.
文摘Background: The medical community has been trying to reduce the high mortality rate of cardiovascular diseases and find effective treatments;however, the results have not been satisfactory. Zhuangtongyin (ZTY), a Chinese herbal formula, has received significant attention owing to its efficacy in the treatment of myocardial ischemia. However, there are few reports on the mechanisms of its action, which hinder its widespread dissemination. Recent studies have shown that the effects of ZTY are associated with the NF-κB/NLRP3 inflammasome pathway. In this study, we investigated the underlying mechanisms of ZTY in myocardial ischemia through the NF-κB/NLRP3 inflammasome pathway. Methods: A myocardial ischemia model was established by ligating rats’ left anterior descending coronary artery. The rats were randomly divided into five groups: control group, model group, sham operation group, ZTY group (13.6 g/kg), and NF-κB group (0.12 g/kg). All groups, except the control group, received oral administration for 28 days. The NF-κB group received peritoneal injection of PDTC for 28 days. ECG, assessment of myocardial ischemia injury, histological examination, and analysis of biochemical indices of myocardial tissue were performed to evaluate myocardial injury and drug protection. Inflammation levels were assessed by Masson staining and Elisa, and expression of transforming growth factor (TGF-β1), α-smooth muscle actin (α-SMA), and connexin 43 (Cx43) was detected by immunohistochemistry. Results: Compared to the control group, the model group showed obvious abnormalities in myocardial tissue and increased levels of inflammatory cytokines. ZTY therapy inhibited the increases in lactate dehydrogenase (LDH), creatinine kinase (CK), creatinine kinase isoenzyme (CK-MB), tumour necrosis factor α (TNF-α), and interleukin-6 (IL-6). The immunohistochemical results showed that ZTY treatment inhibited TGF-β1 expression, suggesting that the anti-inflammatory effect of ZTY was achieved by inhibiting NF-κB signalling. Conclusion: ZTY can effectively alleviate myocardial injury, and its protective effect against myocardial injury is related to inhibition of the NF-κB/NLRP3 inflammasome pathway.
文摘Pineapple industry has played important roles in China’s economics in tropical- and subtropical-areas. Pineapple industry in China has been damaged by water-heart fruit. The objective of this research is to clarify the mechanism underlying water-heart fruit of pineapple and find ways for preventing water-heart fruit. We found that water logging can cause water-heart pineapple. The longer the pineapple plants were water logged, the more fruits were found water heart. With the development of fruits, the fruits’ sensitivity to water logging increased. Spraying potassium sulfate onto the fruits after the plants are water logged can decrease the rate of pineapple water-heart. But this effect became less with the fruit development. When sucrose and its precursors in intracellular space were transferred into sink cells, hydrogen ions were required. Because of lacking hydrogen ions, sucrose and its precursors were stopped in intracellular space and seized water from cells. Water-heart fruit formed. If potassium can be gotten in intracellular space, the roles of hydrogen ions might be replaced with potassium ions. With the help of potassium ions, sucrose and its precursors were transported into sink cells and the water-heart symptoms alleviated.
文摘This paper studies the effect of option trading on corporate investment and financing policies.Based on prior literature,I hypothesize that option market induces informed trading and thus reduces information asymmetry and the cost of capital.As a result,firms with high option trading have more investment and financing.Specifically,based on the United States public data,this paper finds that option trading volume increases corporate investment and financing,but reduces cash holdings and corporate payouts.These results are robust to the inclusion of industry or firm fixed effect,a control for endogenous options trading,and the use of alternative measures of option trading and corporate policies.The effect of option trading is stronger for firms with higher information asymmetry problems.Finally,this paper finds the results are inconsistent with the“quiet Life”hypothesis and the catering hypothesis.
文摘It is critical to combat tumor metastasis by eradicating disseminated tumor cells in any step during the metastasis process.After entering the blood circulation system,tumor cells are in suspension and experience considerable levels of fluid shear stress.However,the influence of hemodynamic shear stress on the survival of CTCs and the underlying mechanotransduction mechanisms remain unclear.This study shows that fluid shear stress can eliminate the majority of CTCs and the viability of suspended tumor cells depends on the stress magnitude,indicating that tumor cells can sense and respond to fluid shear stress.Mechanistically,the expression of Piezo1 but not Piezo2 is greatly upregulated in suspended tumor cells after shear stress treatment.Inhibiting/activating Piezo1 increases/decreases the viability of suspended tumor cells in shear flow,which depends on Piezo1-mediated calcium entry.These findings suggest that Piezo1 may be the major mechanosensor by which suspended tumor cells sense fluid shear stress.As the downstream effector of Piezo1,actomyosin in tumor cells is significantly activated under increasing shear stress.Its activity influences the survival of CTCs in shear flow and rescues the effects of Piezo1 on tumor cell survival,suggesting that hemodynamic shear stress regulates the survival of CTCs through Piezo1 mediated actomyosin activity.Importantly,fluid shear stress considerably up-regulates YAP/TAZ activity of suspended tumor cells and promotes their nuclear translocation in a magnitude-dependent manner.Inhibiting YAP/TAZ enhances the viability of suspended tumor cells in shear stress,while activating their activity decreases tumor cell survival,suggesting that YAP/TAZ activation promotes the apoptosis of suspended tumor cells,which is different from the findings that YAP/TAZ facilitates the survival of adherent cells to shear flow.Further,blocking the nuclear import of YAP/TAZ inactivates the sensation of suspended tumor cells to fluid shear flow and attenuates the dependence of tumor cell survival on different magnitudes of hemodynamic shear stress.The influence of Piezo1-actomyosin pathway on suspended tumor cells can be rescued by YAP/TAZ activity,suggesting that Piezo1-mediated signaling induces tumor cell apoptosis via nuclear translocation of YAP/TAZ.In addition,fluid shear stress can also activate the expressions of LATS1/2 and MST1/2 in Hippo pathway through Piezo1,which is known to inhibit YAP/TAZ activity.Silencing/activating LATS1/2 or MST1/2 inhibits/enhances the viability of CTCs under shear stress,the effects of which can be further rescued by YAP/TAZ.These findings suggest that the responses of suspended tumor cells to hemodynamic shear stress are partially mediated by Hippo signaling.After nuclear translocation,YAP/TAZ directly bind p73/PUMA,which further promotes the transcription of pro-apoptotic genes and induces the apoptosis of suspended tumor cells.In summary,these findings show that hemodynamic shear stress considerably influences the survival of CTCs in blood circulation.We have identified the calcium channel Piezo1 as a novel mechanosensor for the response of CTCs to fluid shear stress.Hemodynamic shear stress induces the apoptosis of suspended tumor cells through Piezo1-actomyosin-YAP/TAZ-p73/PUMA signaling,which is different from the mechanotranduction mechanisms for tumor cells in adherent.Therefore,this study has unveiled the novel mechanosensor of suspended CTCs in response to fluid shear stress and the subsequent mechanisms and identified Piezo1 and YAP/TAZ as the potential therapeutic targets,through which CTCs may be effectively eradicated in the vasculature to prohibit tumor metastasis.
基金support from the National Natural Science Foundation of China(project no.11972316)Shenzhen Science and Technology Innovation Commission(project nos.JCYJ20200109142001798,SGDX2020110309520303,and JCYJ20220531091002006)+3 种基金General Research Fund of Hong Kong Research Grant Council(PolyU 15214320)Health and Medical Research Fund(HMRF18191421)the Research Institute for Smart Ageing in Hong Kong Polytechnic University(1-CD75)Hong Kong Polytechnic University(1-ZE2M,1-ZVY1).
文摘Tumor cells progressively remodel cytoskeletal structures and reduce cellular stiffness during tumor progression,implicating the correlation between cell mechanics and malignancy.However,the roles of tumor cell cytoskeleton and the mechanics in tumor progression remain incompletely understood.We report that softening/stiffening tumor cells by targeting actomyosin promotes/suppresses self-renewal in vitro and tumorigenic potential in vivo.Weakening/strengthening actin cytoskeleton impairs/reinforces the interaction between adenomatous polyposis coli(APC)andβ-catenin,which facilitatesβ-catenin nuclear/cytoplasmic localization.Nuclearβ-catenin binds to the promoter of Oct4,which enhances its transcription that is crucial in sustaining self-renewal and malignancy.These results demonstrate that the mechanics of tumor cells dictate self-renewal through cytoskeleton-APC-Wnt/β-catenin-Oct4 signaling,which are correlated with tumor differentiation and patient survival.This study unveils an uncovered regulatory role of cell mechanics in self-renewal and malignancy,and identifies tumor cell mechanics as a hallmark not only for cancer diagnosis but also for mechanotargeting.
基金supported by the National Natural Science Foundation of China(Grant Nos.22379105 and 22102112)the Natural Science Foundation of Shanxi Province(Grant Nos.20210302123110)。
文摘The use of conjugated microporous polymers(CMPs)in photocatalytic CO_(2)reduction(CO_(2)RR),leveraging solar energy and water to generate carbon-based products,is attracting considerable attention.However,the amorphous nature of most CMPs poses challenges for effective charge carrier separation,limiting their application in CO_(2)RR.In this study,we introduce an innovative approach utilizing donorπ-skeleton engineering to enhance skeleton coplanarity,thereby achieving highly crystalline CMPs.Advanced femtosecond transient absorption and temperature-dependent photoluminescence analyses reveal efficient exciton dissociation into free charge carriers that actively engage in surface reactions.Complementary theoretical calculations demonstrate that our highly crystalline CMP(Py-TDO)not only greatly improves the separation and transfer of photoexcited charge carriers but also introduces additional charge transport pathways via intermolecularπ-πstacking.Py-TDO exhibits outstanding photocatalytic CO_(2) reduction capabilities,achieving a remarkable CO generation rate of 223.97μmol g^(-1)h^(-1)without the addition of chemical scavengers.This work lays pioneering groundwork for the development of novel highly crystalline materials,advancing the field of solar-driven energy conversion.
