Wetlands are critical nature-based solutions advancing climate mitigation,water security,and biodiversity conservation targets.Their ongoing degradation undermines climate resilience,ecosystem integrity,economic stabi...Wetlands are critical nature-based solutions advancing climate mitigation,water security,and biodiversity conservation targets.Their ongoing degradation undermines climate resilience,ecosystem integrity,economic stability,and hu-man health at multiple scales.Against this urgent backdrop,the 15th Confer-ence of the Contracting Parties(COP15)to the Ramsar Convention on Wetlands convened 1,284 delegates from 172 parties in Victoria Falls,Zimbabwe,from July 23rd to 31st,2025,under the theme“Wetlands Action for People and Na-ture”to reinforce global commitment to wetland conservation,restoration,and wise use(Figure 1).展开更多
An 8-week feeding experiment was carried out to explore the effects of dietary n-3/n-6 polyunsaturated fatty acid(PUFA)ratio on growth performance,lipid metabolism,hepatic antioxidant status,and gut flora of spotted s...An 8-week feeding experiment was carried out to explore the effects of dietary n-3/n-6 polyunsaturated fatty acid(PUFA)ratio on growth performance,lipid metabolism,hepatic antioxidant status,and gut flora of spotted seabass(Lateolabrax maculatus).Six experimental diets were formulated to contain different levels of two purified oil sources including docosahexaenoic and eicosapentaenoic acids enriched oil(n-3)and linoleic acid-enriched oil(n-6)leading to n-3/n-6 PUFA ratios of 0.04,0.35,0.66,1.35,2.45 and16.17.Each diet was fed to triplicate groups of juvenile L.maculatus(11.06±0.20 g,30 fish/tank).Final body weight(FBW),weight gain(WG),specific growth rates(SGR),protein efficiency ratio(PER)and feed utilization efficiency increased as n-3/n-6 PUFA ratio increased up to a certain level,and then decreased thereafter.Fish fed the diet with n-3/n-6 PUFA ratio of 0.66 exhibited the highest FBW,WG,SGR and PER and the lowest feed conversion ratio.Lower n-3/n-6 PUFA ratios induced up-regulated expression of lipid synthesis-related genes(fas,acc2 and srebp-1c)and down-regulated expression of lipolysis related genes(atgl,ppara,cpt-1 and aox).Higher expression of lipolysis-related genes(atgl,ppara and cpt-1)was recorded at moderate n-3/n-6 PUFA ratios(0.66 to 1.35).Moreover,inappropriate n-3/n-6 PUFA ratios triggered up-regulation of pro-inflammatory genes(il-6 and tnf-a)and down-regulation of antiinflammatory genes(il-4 and il-10)in the intestine.The diet with n-3/n-6 PUFA ratio of 0.66 inhibited intestine inflammation,improved intestinal flora richness,increased the abundance of beneficial bacteria such as Lactobacillus,Alloprevotella and Ruminococcus,and reduced the abundance of harmful bacteria including Escherichia-Shigella and Enterococcus.In summary,it could be suggested that a dietary n-3/n-6PUFA ratio of 0.66 can improve growth performance and feed utilization in L.maculatus,as is deemed to be mediated through regulation of lipid metabolism and intestinal flora.展开更多
Intensive aquaculture-induced oxidative stress is detrimental to fish health and yield.Medicinal plants show promise as natural health boosters and antioxidants in the aquaculture industry.Therefore,this work investig...Intensive aquaculture-induced oxidative stress is detrimental to fish health and yield.Medicinal plants show promise as natural health boosters and antioxidants in the aquaculture industry.Therefore,this work investigated the effects of turmeric aqueous extract(TAE)on the growth performance,antioxidant status,and hydrogen peroxide(H2O2)-induced oxidative stress in spotted seabass(Lateolabrax maculatus).Fish were fed diets supplemented with 0(Con),2(TAE2),or 4(TAE4)g/kg TAE for eight weeks,then were injected with H2O2.The results showed that dietary supplementation of TAE did not affect fish growth,feed utilization,or body composition.TAE treatment increased liver antioxidant enzyme activities and decreased liver malondialdehyde content and serum levels of glutamate oxalate transaminase,glutamate pyruvate transaminase,and lactate dehydrogenase.Furthermore,the increases in mortality,liver malondialdehyde content,and serum biomarkers of liver injury in the H2O2-treated fish were inhibited as a consequence of the TAE treatment.In addition,TAE treatment activated the Nrf2/Keap1 pathway in the liver,supported by the up-regulated expression of nrf2,ho-1,and gclc,and down-regulated keap1 expression.Overall,dietary incorporation of TAE protected against H2O2-induced oxidative stress in spotted seabass probably by enhancing antioxidant capacity through the Nrf2/Keap1 pathway.展开更多
The present study was conducted to determine the effects of dietary phosphorus(P)levels on growth performance,body composition,liver histology and enzymatic activity,and expression of lipid metabolism-related genes in...The present study was conducted to determine the effects of dietary phosphorus(P)levels on growth performance,body composition,liver histology and enzymatic activity,and expression of lipid metabolism-related genes in spotted seabass(Lateolabrax maculatus).Seven diets were prepared to contain available P levels of 0.48%(the control group),0.69%,0.89%,1.10%,1.28%,1.51%and 1.77%and feed fish(4.26±0.03 g)to satiety twice daily for 10 weeks.Significantly higher weight gain and specific growth rate were recorded at P levels of 0.69%-1.51%compared to the control group.Feed conversion ratio decreased with increasing P levels up to 0.89%and increased thereafter.The lowest liver lipid content,viscerosomatic index and lipid content of whole-body were obtained in the 0.89%-P group among dietary treatments.P and calcium(Ca)contents in whole body were increased,while liver triglyceride and cholesterol contents were decreased with increasing dietary P levels from 0.48%to 1.77%.The highest activity of hepatic lipase was recorded in the 1.10%-P group among dietary treatments.Compared to the control group,1.10%P enhanced the proportion of HUFA and reduced the proportion of SFA and MUFA.The histological observations showed that P deficiency(0.48%)led to the vacuolization of hepatocytes and increased number of lipid droplets.Meanwhile,overall liver tissue structure was improved when P level increased to 1.28%.Compared to the control group,expression of lipid metabolism-related genes such as FAS,ACC-2 and SREBP-1 was decreased at 0.89%-1.10%P group while an opposite trend was observed in the expression of PPARa2 and CPT-1 genes.The current study showed that 0.89%dietary P levels could promote growth performance of spotted seabass and reduce lipid accumulation in the liver.A broken-line regression analysis based on weight gain showed that the optimum dietary P level(available P)for juvenile spotted seabass reared in freshwater was 0.72%.展开更多
A 10-week feeding trial,followed by 24-h nitrite stress,was performed to evaluate the effects of dietary selenium-L-methionine(Se-Met)on growth,Se accumulation,antioxidant capacity,transcripts of selenoproteins and hi...A 10-week feeding trial,followed by 24-h nitrite stress,was performed to evaluate the effects of dietary selenium-L-methionine(Se-Met)on growth,Se accumulation,antioxidant capacity,transcripts of selenoproteins and histological changes of muscle as well as resistance to nitrite stress in spotted seabass(Lateolabrax maculatus)reared at optimal(27℃)and high(33℃)temperatures.Five experimental diets were formulated to contain Se-Met at 0,0.9,1.8,3.5,and 7.0 mg/kg.Each diet was fed to fish(2.60±0.2 g)in two parallel treatments at 27 or 33℃.The results showed that elevated temperature(33℃)induced thermal stress in fish,and fish under thermal stress exhibited lower weight gain and hepatosomatic index but a higher condition factor compared to those reared at 27℃.However,the growth and feed utilisation were promoted in L.maculatus with 0.9 to 3.5 mg/kg Se-Met treatments.The protein and lipid content in the muscle increased with the dietary Se-Met level,and the total Se level in the whole body and muscle showed a linear increase with dietary Se-Met supplementation.Thermal stress changed the histology of the muscle,leading to raised levels of malondialdehyde(MDA),reduced antioxidant parameters in the serum and liver,and a decrease in the transcripts of selenoprotein genes in the muscle.Meanwhile,increased antioxidant capacity of serum and liver and up-regulated transcripts of selenoprotein of muscle were observed in L.maculatus reaching a maximum with 3.5 mg Se-Met/kg treatment.After 24 h of nitrite stress,thermal stress exacerbated oxidative damage caused by nitrite stress in L.maculatus.In contrast,dietary Se-Met enhanced the resistance to nitrite stress of L.maculatus fed with Se-Met enriched diets containing 0.9 to 1.8 mg Se-Met/kg.Based on the effects of dietary Se-Met on the growth,antioxidant capacity and resistance to nitrite stress of L.maculatus,this study suggests that the optimal range of Se-Met supplementation in L.maculatus diets is 1.80 to 2.39 mg Se-Met/kg of diet at 27℃ and 1.80 to 4.46 mg Se-Met/kg of diet at 33℃.展开更多
基金supported by the National Key Research and Development Program of China(2022YFF1300900)the National Natural Science Foundation of China(42430511)the Science and Technology Development Program of Jilin Province,China(20230101348JC).
文摘Wetlands are critical nature-based solutions advancing climate mitigation,water security,and biodiversity conservation targets.Their ongoing degradation undermines climate resilience,ecosystem integrity,economic stability,and hu-man health at multiple scales.Against this urgent backdrop,the 15th Confer-ence of the Contracting Parties(COP15)to the Ramsar Convention on Wetlands convened 1,284 delegates from 172 parties in Victoria Falls,Zimbabwe,from July 23rd to 31st,2025,under the theme“Wetlands Action for People and Na-ture”to reinforce global commitment to wetland conservation,restoration,and wise use(Figure 1).
基金funded by the National Natural Science Foundation of China(32072984)the Natural Science Foundation of Fujian Province(2020J01664)China Agricultural Research System(CARS-47)。
文摘An 8-week feeding experiment was carried out to explore the effects of dietary n-3/n-6 polyunsaturated fatty acid(PUFA)ratio on growth performance,lipid metabolism,hepatic antioxidant status,and gut flora of spotted seabass(Lateolabrax maculatus).Six experimental diets were formulated to contain different levels of two purified oil sources including docosahexaenoic and eicosapentaenoic acids enriched oil(n-3)and linoleic acid-enriched oil(n-6)leading to n-3/n-6 PUFA ratios of 0.04,0.35,0.66,1.35,2.45 and16.17.Each diet was fed to triplicate groups of juvenile L.maculatus(11.06±0.20 g,30 fish/tank).Final body weight(FBW),weight gain(WG),specific growth rates(SGR),protein efficiency ratio(PER)and feed utilization efficiency increased as n-3/n-6 PUFA ratio increased up to a certain level,and then decreased thereafter.Fish fed the diet with n-3/n-6 PUFA ratio of 0.66 exhibited the highest FBW,WG,SGR and PER and the lowest feed conversion ratio.Lower n-3/n-6 PUFA ratios induced up-regulated expression of lipid synthesis-related genes(fas,acc2 and srebp-1c)and down-regulated expression of lipolysis related genes(atgl,ppara,cpt-1 and aox).Higher expression of lipolysis-related genes(atgl,ppara and cpt-1)was recorded at moderate n-3/n-6 PUFA ratios(0.66 to 1.35).Moreover,inappropriate n-3/n-6 PUFA ratios triggered up-regulation of pro-inflammatory genes(il-6 and tnf-a)and down-regulation of antiinflammatory genes(il-4 and il-10)in the intestine.The diet with n-3/n-6 PUFA ratio of 0.66 inhibited intestine inflammation,improved intestinal flora richness,increased the abundance of beneficial bacteria such as Lactobacillus,Alloprevotella and Ruminococcus,and reduced the abundance of harmful bacteria including Escherichia-Shigella and Enterococcus.In summary,it could be suggested that a dietary n-3/n-6PUFA ratio of 0.66 can improve growth performance and feed utilization in L.maculatus,as is deemed to be mediated through regulation of lipid metabolism and intestinal flora.
基金supported by China Agriculture Research System (CARS-47)the Natural Science Foundation of Fujian Province of China (grant number:2019J01060380).
文摘Intensive aquaculture-induced oxidative stress is detrimental to fish health and yield.Medicinal plants show promise as natural health boosters and antioxidants in the aquaculture industry.Therefore,this work investigated the effects of turmeric aqueous extract(TAE)on the growth performance,antioxidant status,and hydrogen peroxide(H2O2)-induced oxidative stress in spotted seabass(Lateolabrax maculatus).Fish were fed diets supplemented with 0(Con),2(TAE2),or 4(TAE4)g/kg TAE for eight weeks,then were injected with H2O2.The results showed that dietary supplementation of TAE did not affect fish growth,feed utilization,or body composition.TAE treatment increased liver antioxidant enzyme activities and decreased liver malondialdehyde content and serum levels of glutamate oxalate transaminase,glutamate pyruvate transaminase,and lactate dehydrogenase.Furthermore,the increases in mortality,liver malondialdehyde content,and serum biomarkers of liver injury in the H2O2-treated fish were inhibited as a consequence of the TAE treatment.In addition,TAE treatment activated the Nrf2/Keap1 pathway in the liver,supported by the up-regulated expression of nrf2,ho-1,and gclc,and down-regulated keap1 expression.Overall,dietary incorporation of TAE protected against H2O2-induced oxidative stress in spotted seabass probably by enhancing antioxidant capacity through the Nrf2/Keap1 pathway.
基金This study was supported by National Natural Science Foundation of China(grant number:31972804)the China Agricultural Research System(grant number:CARS47-14).
文摘The present study was conducted to determine the effects of dietary phosphorus(P)levels on growth performance,body composition,liver histology and enzymatic activity,and expression of lipid metabolism-related genes in spotted seabass(Lateolabrax maculatus).Seven diets were prepared to contain available P levels of 0.48%(the control group),0.69%,0.89%,1.10%,1.28%,1.51%and 1.77%and feed fish(4.26±0.03 g)to satiety twice daily for 10 weeks.Significantly higher weight gain and specific growth rate were recorded at P levels of 0.69%-1.51%compared to the control group.Feed conversion ratio decreased with increasing P levels up to 0.89%and increased thereafter.The lowest liver lipid content,viscerosomatic index and lipid content of whole-body were obtained in the 0.89%-P group among dietary treatments.P and calcium(Ca)contents in whole body were increased,while liver triglyceride and cholesterol contents were decreased with increasing dietary P levels from 0.48%to 1.77%.The highest activity of hepatic lipase was recorded in the 1.10%-P group among dietary treatments.Compared to the control group,1.10%P enhanced the proportion of HUFA and reduced the proportion of SFA and MUFA.The histological observations showed that P deficiency(0.48%)led to the vacuolization of hepatocytes and increased number of lipid droplets.Meanwhile,overall liver tissue structure was improved when P level increased to 1.28%.Compared to the control group,expression of lipid metabolism-related genes such as FAS,ACC-2 and SREBP-1 was decreased at 0.89%-1.10%P group while an opposite trend was observed in the expression of PPARa2 and CPT-1 genes.The current study showed that 0.89%dietary P levels could promote growth performance of spotted seabass and reduce lipid accumulation in the liver.A broken-line regression analysis based on weight gain showed that the optimum dietary P level(available P)for juvenile spotted seabass reared in freshwater was 0.72%.
基金supported by the Agriculture Research System of China(CARS-47)the Natural Science Foundation of Fujian Province of China(grant number:2020J01665,2023J01766).
文摘A 10-week feeding trial,followed by 24-h nitrite stress,was performed to evaluate the effects of dietary selenium-L-methionine(Se-Met)on growth,Se accumulation,antioxidant capacity,transcripts of selenoproteins and histological changes of muscle as well as resistance to nitrite stress in spotted seabass(Lateolabrax maculatus)reared at optimal(27℃)and high(33℃)temperatures.Five experimental diets were formulated to contain Se-Met at 0,0.9,1.8,3.5,and 7.0 mg/kg.Each diet was fed to fish(2.60±0.2 g)in two parallel treatments at 27 or 33℃.The results showed that elevated temperature(33℃)induced thermal stress in fish,and fish under thermal stress exhibited lower weight gain and hepatosomatic index but a higher condition factor compared to those reared at 27℃.However,the growth and feed utilisation were promoted in L.maculatus with 0.9 to 3.5 mg/kg Se-Met treatments.The protein and lipid content in the muscle increased with the dietary Se-Met level,and the total Se level in the whole body and muscle showed a linear increase with dietary Se-Met supplementation.Thermal stress changed the histology of the muscle,leading to raised levels of malondialdehyde(MDA),reduced antioxidant parameters in the serum and liver,and a decrease in the transcripts of selenoprotein genes in the muscle.Meanwhile,increased antioxidant capacity of serum and liver and up-regulated transcripts of selenoprotein of muscle were observed in L.maculatus reaching a maximum with 3.5 mg Se-Met/kg treatment.After 24 h of nitrite stress,thermal stress exacerbated oxidative damage caused by nitrite stress in L.maculatus.In contrast,dietary Se-Met enhanced the resistance to nitrite stress of L.maculatus fed with Se-Met enriched diets containing 0.9 to 1.8 mg Se-Met/kg.Based on the effects of dietary Se-Met on the growth,antioxidant capacity and resistance to nitrite stress of L.maculatus,this study suggests that the optimal range of Se-Met supplementation in L.maculatus diets is 1.80 to 2.39 mg Se-Met/kg of diet at 27℃ and 1.80 to 4.46 mg Se-Met/kg of diet at 33℃.
