Continuous cropping can lead to soil environment deterioration,cause plant health problems,and reduce crop productivity.However,the response mechanisms of soil microbial co-occurrence patterns to the duration of conti...Continuous cropping can lead to soil environment deterioration,cause plant health problems,and reduce crop productivity.However,the response mechanisms of soil microbial co-occurrence patterns to the duration of continuous melon cropping remain poorly understood.Here,we employed the metagenomic techniques to comparatively investigate the bulk and rhizosphere soil microbial communities of major melon-producing regions(where the duration of continuous melon cropping ranges from 1 to 30 a)in the eastern and southern parts of Xinjiang Uygur Autonomous Region,China.The results showed that soil pH clearly decreased with increasing melon cropping duration,while soil electrical conductivity(EC)and the other soil nutrient indices increased with increasing melon cropping duration(with the exception of AN and TK in the southern melon-producing region).The most dominant bacterial phyla were Proteobacteria and Actinobacteria,and the most abundant fungal phyla were Ascomycota and Mucoromycota.Redundancy analysis(RDA)indicated that soil pH and EC had no significant effects on the bacterial communities.However,after many years of continuous melon cropping in the southern melon-producing region,fungal communities were significantly negatively correlated with soil pH and significantly positively correlated with soil EC(P<0.050).Co-occurrence network analysis showed that continuous melon cropping increased the complexity but decreased the connectivity of the cross-domain microbial networks.Moreover,the enrichment patterns of microorganisms in the main microbial network modules varied significantly with the duration of continuous melon cropping.Based on the analysis of keystone taxa,we found that continuous melon cropping increased some plant pathogens(e.g.,Fusarium and Stagonospora)but decreased beneficial bacteria(e.g.,Mesorhizobium and Pseudoxanthomonas).In conclusion,this study has greatly enhanced the understanding of the effects of continuous melon cropping on alterations in the microbial community structure and ecological networks in Xinjiang.展开更多
基金funded by the Major Science and Technology Projects of Xinjiang Uygur Autonomous Region(2022A02007-4)the Xinjiang Uygur Autonomous Region Natural Science Foundation Youth Project(2024D01B31)the Graduate Student Research Innovation Project of Xinjiang Agricultural University(XJAUGRI2024033).
文摘Continuous cropping can lead to soil environment deterioration,cause plant health problems,and reduce crop productivity.However,the response mechanisms of soil microbial co-occurrence patterns to the duration of continuous melon cropping remain poorly understood.Here,we employed the metagenomic techniques to comparatively investigate the bulk and rhizosphere soil microbial communities of major melon-producing regions(where the duration of continuous melon cropping ranges from 1 to 30 a)in the eastern and southern parts of Xinjiang Uygur Autonomous Region,China.The results showed that soil pH clearly decreased with increasing melon cropping duration,while soil electrical conductivity(EC)and the other soil nutrient indices increased with increasing melon cropping duration(with the exception of AN and TK in the southern melon-producing region).The most dominant bacterial phyla were Proteobacteria and Actinobacteria,and the most abundant fungal phyla were Ascomycota and Mucoromycota.Redundancy analysis(RDA)indicated that soil pH and EC had no significant effects on the bacterial communities.However,after many years of continuous melon cropping in the southern melon-producing region,fungal communities were significantly negatively correlated with soil pH and significantly positively correlated with soil EC(P<0.050).Co-occurrence network analysis showed that continuous melon cropping increased the complexity but decreased the connectivity of the cross-domain microbial networks.Moreover,the enrichment patterns of microorganisms in the main microbial network modules varied significantly with the duration of continuous melon cropping.Based on the analysis of keystone taxa,we found that continuous melon cropping increased some plant pathogens(e.g.,Fusarium and Stagonospora)but decreased beneficial bacteria(e.g.,Mesorhizobium and Pseudoxanthomonas).In conclusion,this study has greatly enhanced the understanding of the effects of continuous melon cropping on alterations in the microbial community structure and ecological networks in Xinjiang.
