This paper describes a method to estimate the direction from which the signal molecule reaches the sensor by using living cells.In this context,biohybrid sensors that utilize a sophisticated sensing system of cells ca...This paper describes a method to estimate the direction from which the signal molecule reaches the sensor by using living cells.In this context,biohybrid sensors that utilize a sophisticated sensing system of cells can potentially offer high levels of chemicaldetection sensitivity and selectivity.However,biohybrid-sensor-based chemical-source-direction estimation has not received research attention because the cellular response to chemicals has not been examined in the context of directional information.In our approach,we fabricated a device that can limit the interface between the cell-laden hydrogel and the chemical solution of interest to enhance the time difference over which the chemical solution reaches the cells.Chemical detection by cells that express specific receptors is reflected as the fluorescence of the calcium indicator within the cells.Our device has eight chambers that each house 3D cell-laden collagen hydrogels facing circularly outward.The device also works as a cover to prevent chemicals from permeating the hydrogel from above.In our study,by observing the time course of the fluorescence emission of each chamber,we were able to successfully estimate the chemical-source direction within an error range of 7-13°.Our results suggest that a combination of microstructure devices embedded with living cells can be used to exploit cell functionalities to yield chemical-source directional information.展开更多
基金supported by the New Energy and Industrial Technology Development Organization(NEDO)and a JSPS Grantsin-Aid for Scientific(KAKENHI)(Grant Number 19H05322).
文摘This paper describes a method to estimate the direction from which the signal molecule reaches the sensor by using living cells.In this context,biohybrid sensors that utilize a sophisticated sensing system of cells can potentially offer high levels of chemicaldetection sensitivity and selectivity.However,biohybrid-sensor-based chemical-source-direction estimation has not received research attention because the cellular response to chemicals has not been examined in the context of directional information.In our approach,we fabricated a device that can limit the interface between the cell-laden hydrogel and the chemical solution of interest to enhance the time difference over which the chemical solution reaches the cells.Chemical detection by cells that express specific receptors is reflected as the fluorescence of the calcium indicator within the cells.Our device has eight chambers that each house 3D cell-laden collagen hydrogels facing circularly outward.The device also works as a cover to prevent chemicals from permeating the hydrogel from above.In our study,by observing the time course of the fluorescence emission of each chamber,we were able to successfully estimate the chemical-source direction within an error range of 7-13°.Our results suggest that a combination of microstructure devices embedded with living cells can be used to exploit cell functionalities to yield chemical-source directional information.
