Correction to:Radiation Detection Technology and Methods(2024)8:1-1105.https://doi.org/10.1007/s41605-024-00463-y.In this article all authors name was missing in the springer link.It has been corrected.The original ar...Correction to:Radiation Detection Technology and Methods(2024)8:1-1105.https://doi.org/10.1007/s41605-024-00463-y.In this article all authors name was missing in the springer link.It has been corrected.The original article has been corrected.展开更多
Calcium acts as a second messenger for signaling to a variety of stimuli including MAMPs （Microbe-Associated Molecular Patterns）, such as fig22 and elf18 that are derived from bacterial flagellin and elongation fact...Calcium acts as a second messenger for signaling to a variety of stimuli including MAMPs （Microbe-Associated Molecular Patterns）, such as fig22 and elf18 that are derived from bacterial flagellin and elongation factor Tu, respectively. Here, Arabidopsis thaliana mutants with changed calcium elevation （cce） in response to fig22 treatment were isolated and characterized. Besides novel mutant alleles of the fig22 receptor, FLS2 （Flagellin-Sensitive 2）, and the receptor-associated kinase, BAK1 （Brassinosteroid receptor 1-Associated Kinase 1）, the new cce mutants can be categorized into two main groups--those with a reduced or an enhanced calcium elevation. Moreover, cce mutants from both groups show differ- ential phenotypes to different sets of MAMPs. Thus, these mutants will facilitate the discovery of novel components in early MAMP signaling and bridge the gaps in current knowledge of calcium signaling during plant-microbe interactions. Last but not least, the screening method is optimized for speed （covering 384 plants in 3 or 10 h） and can be adapted to genetically dissect any other stimuli that induce a change in calcium levels.展开更多
The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international partners.The complex comprises four accelerators:a 3...The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international partners.The complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs factory.In its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs bosons.Increasing the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of magnitude.This Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering aspects.Additionally,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection procedures.Construction is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 years.The commencement of experiments and data collection could potentially be initiated in the mid-2030s.展开更多
Proteome remodeling is a fundamental adaptive response,and proteins in complexes and functionally related proteins are often co-expressed.Using a deep sampling strategy we define core proteomes of Arabidopsis thaliana...Proteome remodeling is a fundamental adaptive response,and proteins in complexes and functionally related proteins are often co-expressed.Using a deep sampling strategy we define core proteomes of Arabidopsis thaliana tissues with around 10 000 proteins per tissue,and absolutely quantify(copy numbers per cell)nearly 16 000 proteins throughout the plant lifecycle.A proteome-wide survey of global post-trans-lational modification revealed amino acid exchanges pointing to potential conservation of translational infi-delity in eukaryotes.Correlation analysis of protein abundance uncovered potentially new tissue-and age-specific roles of entire signaling modules regulating transcription in photosynthesis,seed development,and senescence and abscission.Among others,the data suggest a potential function of RD26 and other NAC transcription factors in seed development related to desiccation tolerance as well as a possible function of cysteine-rich receptor-like kinases(CRKs)as ROS sensors in senescence.All of the components of ribo-some biogenesis factor(RBF)complexes were found to be co-expressed in a tissue-and age-specific manner,indicating functional promiscuity in the assembly of these less-studied protein complexes in Arabidopsis.Furthermore,we characterized detailed proteome remodeling in basalimmunity bytreatingAra-bidopsis seeldings with flg22.Through simultaneously monitoring phytohormone and transcript changes upon flg22 treatment,we obtained strong evidence of suppression of jasmonate(JA)and JA-isoleucine(JA-Ile)levels by deconjugation and hydroxylation by IAA-ALA RESISTANT3(IAR3)and JASMONATE-INDUCED OXYGENASE 2(JOX2),respectively,under the control of JASMONATE INSENSITIVE 1(MYC2),sug-gesting an unrecognized role of a new JA regulatory switch in pattern-triggered immunity.Taken together,the datasets generated in this study present extensive coverage of theArabidopsis proteome in various bio-logical scenarios,providing a rich resource available to the whole plant science community.展开更多
文摘Correction to:Radiation Detection Technology and Methods(2024)8:1-1105.https://doi.org/10.1007/s41605-024-00463-y.In this article all authors name was missing in the springer link.It has been corrected.The original article has been corrected.
基金This work was supported by a Deutsche Forschungsgemeinschaft (DFG) grant，financed by the DFG grant，by the Swiss National Science Foundation grant
文摘Calcium acts as a second messenger for signaling to a variety of stimuli including MAMPs （Microbe-Associated Molecular Patterns）, such as fig22 and elf18 that are derived from bacterial flagellin and elongation factor Tu, respectively. Here, Arabidopsis thaliana mutants with changed calcium elevation （cce） in response to fig22 treatment were isolated and characterized. Besides novel mutant alleles of the fig22 receptor, FLS2 （Flagellin-Sensitive 2）, and the receptor-associated kinase, BAK1 （Brassinosteroid receptor 1-Associated Kinase 1）, the new cce mutants can be categorized into two main groups--those with a reduced or an enhanced calcium elevation. Moreover, cce mutants from both groups show differ- ential phenotypes to different sets of MAMPs. Thus, these mutants will facilitate the discovery of novel components in early MAMP signaling and bridge the gaps in current knowledge of calcium signaling during plant-microbe interactions. Last but not least, the screening method is optimized for speed （covering 384 plants in 3 or 10 h） and can be adapted to genetically dissect any other stimuli that induce a change in calcium levels.
基金support from diverse funding sources,including the National Key Program for S&T Research and Development of the Ministry of Science and Technology(MOST),Yifang Wang's Science Studio of the Ten Thousand Talents Project,the CAS Key Foreign Cooperation Grant,the National Natural Science Foundation of China(NSFC)Beijing Municipal Science&Technology Commission,the CAS Focused Science Grant,the IHEP Innovation Grant,the CAS Lead Special Training Programthe CAS Center for Excellence in Particle Physics,the CAS International Partnership Program,and the CAS/SAFEA International Partnership Program for Creative Research Teams.
文摘The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international partners.The complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs factory.In its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs bosons.Increasing the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of magnitude.This Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering aspects.Additionally,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection procedures.Construction is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 years.The commencement of experiments and data collection could potentially be initiated in the mid-2030s.
基金We thank the Leibniz Association for support and fundingMohammad Abukhalaf is funded by DFG grant HO 5063/2-1+1 种基金Mohamed Ayash is funded by DFG 400681449/GRK2498This work was also supported by de.NBI(FKZ 031 A 534A),a project of the BMBF(Bundesministerium fur Bildung und Forschung).
文摘Proteome remodeling is a fundamental adaptive response,and proteins in complexes and functionally related proteins are often co-expressed.Using a deep sampling strategy we define core proteomes of Arabidopsis thaliana tissues with around 10 000 proteins per tissue,and absolutely quantify(copy numbers per cell)nearly 16 000 proteins throughout the plant lifecycle.A proteome-wide survey of global post-trans-lational modification revealed amino acid exchanges pointing to potential conservation of translational infi-delity in eukaryotes.Correlation analysis of protein abundance uncovered potentially new tissue-and age-specific roles of entire signaling modules regulating transcription in photosynthesis,seed development,and senescence and abscission.Among others,the data suggest a potential function of RD26 and other NAC transcription factors in seed development related to desiccation tolerance as well as a possible function of cysteine-rich receptor-like kinases(CRKs)as ROS sensors in senescence.All of the components of ribo-some biogenesis factor(RBF)complexes were found to be co-expressed in a tissue-and age-specific manner,indicating functional promiscuity in the assembly of these less-studied protein complexes in Arabidopsis.Furthermore,we characterized detailed proteome remodeling in basalimmunity bytreatingAra-bidopsis seeldings with flg22.Through simultaneously monitoring phytohormone and transcript changes upon flg22 treatment,we obtained strong evidence of suppression of jasmonate(JA)and JA-isoleucine(JA-Ile)levels by deconjugation and hydroxylation by IAA-ALA RESISTANT3(IAR3)and JASMONATE-INDUCED OXYGENASE 2(JOX2),respectively,under the control of JASMONATE INSENSITIVE 1(MYC2),sug-gesting an unrecognized role of a new JA regulatory switch in pattern-triggered immunity.Taken together,the datasets generated in this study present extensive coverage of theArabidopsis proteome in various bio-logical scenarios,providing a rich resource available to the whole plant science community.
