The problem of soil polycyclic aromatic hydrocarbon(PAH)pollution in coking plant sites has been widely studied in recent years,but there is a lack of research on the correlation between soil microorganisms,soil metab...The problem of soil polycyclic aromatic hydrocarbon(PAH)pollution in coking plant sites has been widely studied in recent years,but there is a lack of research on the correlation between soil microorganisms,soil metabolomics,and soil properties.Thus,in this study,the long-term impact of coke combustion on soil microbial community structure,enzyme activities,and metabolic pathways within a former coking plant site was investigated.Soil samples were collected from both the coking production area(CA group)and office area(OLA group),approximately 0 to 20 cm in depth.Compared with OLA group,elevated levels of 16 PAHs in the list of US EPA were detected by gas chromatography-mass spectrometry in the CA group.Several dominant microorganisms,such as Altererythrobacter,Lysobacter,and Sulfurifustis,were identified by 16 s ribosomal DNA sequencing in the CA group.The fatty acid biosynthesis pathway exhibited specific inhibition,while the phenylalanine metabolic pathwaywas promoted in response to PAH stress.Long-term PAH exposure led to the inhibition of soil urease activity.The co-occurrence network ofmicroorganisms revealed intricate patterns of co-metabolism and co-adaptation within complex bacterial communities,facilitating their adaptation to and decomposition of soil-borne PAHs.This research could provide valuable insights into the community characteristics andmetabolic mechanisms of microorganisms inhabiting PAH-polluted soil within coking plant sites.The findings enhance our understanding of the indigenous soil microbiome and its intricate network dynamics under the persistent stress of PAHs,contributing to a more comprehensive knowledge of soil ecosystems in such environments.展开更多
基金supported by the National Key Research and Development Program of China(Nos.2018YFA0901100 and 2018YFC1801103)the National Natural Science Foundation of China(Nos.22206202 and 22076216)。
文摘The problem of soil polycyclic aromatic hydrocarbon(PAH)pollution in coking plant sites has been widely studied in recent years,but there is a lack of research on the correlation between soil microorganisms,soil metabolomics,and soil properties.Thus,in this study,the long-term impact of coke combustion on soil microbial community structure,enzyme activities,and metabolic pathways within a former coking plant site was investigated.Soil samples were collected from both the coking production area(CA group)and office area(OLA group),approximately 0 to 20 cm in depth.Compared with OLA group,elevated levels of 16 PAHs in the list of US EPA were detected by gas chromatography-mass spectrometry in the CA group.Several dominant microorganisms,such as Altererythrobacter,Lysobacter,and Sulfurifustis,were identified by 16 s ribosomal DNA sequencing in the CA group.The fatty acid biosynthesis pathway exhibited specific inhibition,while the phenylalanine metabolic pathwaywas promoted in response to PAH stress.Long-term PAH exposure led to the inhibition of soil urease activity.The co-occurrence network ofmicroorganisms revealed intricate patterns of co-metabolism and co-adaptation within complex bacterial communities,facilitating their adaptation to and decomposition of soil-borne PAHs.This research could provide valuable insights into the community characteristics andmetabolic mechanisms of microorganisms inhabiting PAH-polluted soil within coking plant sites.The findings enhance our understanding of the indigenous soil microbiome and its intricate network dynamics under the persistent stress of PAHs,contributing to a more comprehensive knowledge of soil ecosystems in such environments.
