Background:Microvascular invasion(MVI)was a critical high-risk factor for postoperative recurrence and adverse prognosis in patients with hepatocellular carcinoma(HCC),and there were no reliable non-invasive pre-opera...Background:Microvascular invasion(MVI)was a critical high-risk factor for postoperative recurrence and adverse prognosis in patients with hepatocellular carcinoma(HCC),and there were no reliable non-invasive pre-operative diagnostic markers.The exosomal N-glycan profile was closely related to the invasion and immune escape of HCC.Therefore,this article investigated the expression of N-glycan profiles in serum exosomes of patients with HCC and its clinical significance for MVI.Methods:Serum samples from 210 patients with HCC were collected and randomly divided into modeling and validation cohorts.The abundances of N-glycans in serum exosomes with different MVI grades were determined.A diagnostic model for MVI in HCC based on N-glycosylation was constructed and the diagnostic value was analyzed.Results:In the modeling cohort,comparing groups M0 with M2,the area under the receiver operating characteristic(AUC)of the diagnostic model namely SUM(AUCSUM)was 0.861,the sensitivity of SUM was 92.68%,and the specificity of SUM was 79.41%,all of which were higher than the seven individual indexes(containing Peak 1,Peak 6,Peak 9,Peak 10,Peak 12,AFP,and PIVKA-II).In the comparison between the M1 and M2 groups,the AUCSUM was 0.749,the sensitivity of SUM was 79.07%,and the specificity of SUM was 76.60%,all of which were higher than the seven individual indexes.When comparing the M0 and M1 groups,the AUCSUM was 0.712,the sensitivity of SUM was 88.57%,and the specificity of SUM was 65.00%.The AUCSUM and sensitivity of SUM were higher than the seven individual indexes and the specificity of SUM was slightly lower than that of AFP(68.18%)but higher than other individual indexes.The results of the validation cohort were similar to those of the modeling cohort.Conclusion:The SUM model of serum exosomes can serve as an auxiliary diagnostic index for MVI staging in patients with HCC.展开更多
基金Fuzhou“14th Five-Year”Clinical Key Specialty,Grant/Award Number:20220203Natural Science Foundation of Fujian Province,Grant/Award Number:2020J011169。
文摘Background:Microvascular invasion(MVI)was a critical high-risk factor for postoperative recurrence and adverse prognosis in patients with hepatocellular carcinoma(HCC),and there were no reliable non-invasive pre-operative diagnostic markers.The exosomal N-glycan profile was closely related to the invasion and immune escape of HCC.Therefore,this article investigated the expression of N-glycan profiles in serum exosomes of patients with HCC and its clinical significance for MVI.Methods:Serum samples from 210 patients with HCC were collected and randomly divided into modeling and validation cohorts.The abundances of N-glycans in serum exosomes with different MVI grades were determined.A diagnostic model for MVI in HCC based on N-glycosylation was constructed and the diagnostic value was analyzed.Results:In the modeling cohort,comparing groups M0 with M2,the area under the receiver operating characteristic(AUC)of the diagnostic model namely SUM(AUCSUM)was 0.861,the sensitivity of SUM was 92.68%,and the specificity of SUM was 79.41%,all of which were higher than the seven individual indexes(containing Peak 1,Peak 6,Peak 9,Peak 10,Peak 12,AFP,and PIVKA-II).In the comparison between the M1 and M2 groups,the AUCSUM was 0.749,the sensitivity of SUM was 79.07%,and the specificity of SUM was 76.60%,all of which were higher than the seven individual indexes.When comparing the M0 and M1 groups,the AUCSUM was 0.712,the sensitivity of SUM was 88.57%,and the specificity of SUM was 65.00%.The AUCSUM and sensitivity of SUM were higher than the seven individual indexes and the specificity of SUM was slightly lower than that of AFP(68.18%)but higher than other individual indexes.The results of the validation cohort were similar to those of the modeling cohort.Conclusion:The SUM model of serum exosomes can serve as an auxiliary diagnostic index for MVI staging in patients with HCC.
