Background:Sepsis,a type of systemic disease,can impact nearly all organs,tissues and cells.Among them,endothelial cells are amongst thefirst to be affected and respond to the insult.In this study,we investigated the p...Background:Sepsis,a type of systemic disease,can impact nearly all organs,tissues and cells.Among them,endothelial cells are amongst thefirst to be affected and respond to the insult.In this study,we investigated the protective effects of microRNA-30a-5p(miR-30a-5p)on human pulmonary microvascular endothelial cells(HPMECs)treated with lipolysaccharide(LPS).Methods:An in vitro model of sepsis was established in HPMECs with the use of LPS.Transfecting with different tools(mimetic and inhibitor)to modify miR-30a-5p expression.Cell viability,proliferation and apoptosis were detected by the CCK-8 assay,the EdU kit andfluorescence staining,respectively.The autophagy-related protein and mRNA expression,the number of autophagosomes were separately examined through Western blot analysis,qPT-PCR and confocal microscopy.TargetScan and the luciferase reporter assays were used to probe target genes interacting with miR-30a-5p.Results:LPS caused a reduction in the viability and proliferation of HPMECs,as well as an elevation in the number of apoptotic cells.Subsequently,we observed that miR-30a-5p might play a role in preventing LPS-induced inhibition of cell damage and decreasing HPMEC apoptosis,suggesting the potential function of miR-30a-5p in this injury process.Finally,we confirmed that miR-30a-5p exerts its protective effect by regulating cell autophagy,possibly by targeting Beclin-1.Conclusion:Our study provided evidence that autophagy is a crucial aspect in the protective role of miR-30a-5p against LPS-induced HPMEC injury,identifying a promising target for sepsis-related endothelial cell injury.展开更多
To systematically comb and explore the rural poverty reduction effect of traffic infrastructure,the author analyzed the mechanism,main channels and actual effect of rural traffic infrastructure on poverty reduction.It...To systematically comb and explore the rural poverty reduction effect of traffic infrastructure,the author analyzed the mechanism,main channels and actual effect of rural traffic infrastructure on poverty reduction.It revealed that rural traffic infrastructure exerts positive or negative effect on poverty reduction directly or indirectly.The positive channel includes increasing non-agricultural employment opportunities,reducing costs for agricultural production,transportation and labor transfer,increasing availability of social service,and promoting adjustment of agricultural industrial structure and development of rural tourism resources.On the whole,traffic infrastructure has significant positive influence on rural poverty reduction.Finally,it summarized policy recommendations for building pro-poor traffic infrastructure,and came up with vacancy of related research field and future exploration direction.展开更多
Adipose tissue fibrosis,characterized by abnormal extracellular matrix deposition within adipose tissue,signifies a crucial indicator of adipose tissue malfunction,potentially leading to organ tissue dysfunction.Vario...Adipose tissue fibrosis,characterized by abnormal extracellular matrix deposition within adipose tissue,signifies a crucial indicator of adipose tissue malfunction,potentially leading to organ tissue dysfunction.Various factors,including a high-fat diet,non-alcoholic fatty liver disease,and insulin resistance,coincide with adipose tissue fibrosis.MicroRNAs(miRNAs)represent a class of small non-coding RNAs with significant influence on tissue fibrosis through diverse signaling pathways.For instance,in response to a high-fat diet,miRNAs can modulate signaling pathways such as TGF-b/Smad,PI3K/AKT,and PPAR-g to impact adipose tissue fibrosis.Furthermore,miRNAs play roles in inhibiting fibrosis in different contexts:sup-pressing corneal fibrosis via the TGF-b/Smad pathway,mitigating cardiac fibrosis through the VEGF signaling pathway,reducing wound fibrosis via regulation of the MAPK signaling pathway,and diminishing fibrosis post-fat transplantation via involvement in the PDGFR-b signaling pathway.Notably,the secretome released by miRNA-transfected adipose-derived stem cells facilitates targeted delivery of miRNAs to evade host immune rejection,enhancing their anti-fibrotic efficacy.Hence,this study endeavors to elucidate the role and mechanism of miR-NAs in adipose tissue fibrosis and explore the mechanisms and advantages of the secretome released by miRNA-transfected adipose-derived stem cells in combating fibrotic diseases.展开更多
基金This study is supported in part by Zhejiang Provincial Natural Science Foundation of China(Grant No.LTGD23H150001)the Medical and Health Research Project of Zhejiang Province(Grant No.2023KY212)the High-Level Talent Research Project of Hangzhou Vocational&Technical College(Grant No.HZYGCC202229).
文摘Background:Sepsis,a type of systemic disease,can impact nearly all organs,tissues and cells.Among them,endothelial cells are amongst thefirst to be affected and respond to the insult.In this study,we investigated the protective effects of microRNA-30a-5p(miR-30a-5p)on human pulmonary microvascular endothelial cells(HPMECs)treated with lipolysaccharide(LPS).Methods:An in vitro model of sepsis was established in HPMECs with the use of LPS.Transfecting with different tools(mimetic and inhibitor)to modify miR-30a-5p expression.Cell viability,proliferation and apoptosis were detected by the CCK-8 assay,the EdU kit andfluorescence staining,respectively.The autophagy-related protein and mRNA expression,the number of autophagosomes were separately examined through Western blot analysis,qPT-PCR and confocal microscopy.TargetScan and the luciferase reporter assays were used to probe target genes interacting with miR-30a-5p.Results:LPS caused a reduction in the viability and proliferation of HPMECs,as well as an elevation in the number of apoptotic cells.Subsequently,we observed that miR-30a-5p might play a role in preventing LPS-induced inhibition of cell damage and decreasing HPMEC apoptosis,suggesting the potential function of miR-30a-5p in this injury process.Finally,we confirmed that miR-30a-5p exerts its protective effect by regulating cell autophagy,possibly by targeting Beclin-1.Conclusion:Our study provided evidence that autophagy is a crucial aspect in the protective role of miR-30a-5p against LPS-induced HPMEC injury,identifying a promising target for sepsis-related endothelial cell injury.
基金Supported by Project of Asian Development Bank(RETA5947)
文摘To systematically comb and explore the rural poverty reduction effect of traffic infrastructure,the author analyzed the mechanism,main channels and actual effect of rural traffic infrastructure on poverty reduction.It revealed that rural traffic infrastructure exerts positive or negative effect on poverty reduction directly or indirectly.The positive channel includes increasing non-agricultural employment opportunities,reducing costs for agricultural production,transportation and labor transfer,increasing availability of social service,and promoting adjustment of agricultural industrial structure and development of rural tourism resources.On the whole,traffic infrastructure has significant positive influence on rural poverty reduction.Finally,it summarized policy recommendations for building pro-poor traffic infrastructure,and came up with vacancy of related research field and future exploration direction.
基金supported in part by the Zhejiang Provincial Natural Science Foundation of China(No.LTGD24H070006,LTGD23H150001)the Medical and Health Research Project of Zhejiang Province,China(No.2023KY212,2024KY631)the Zhejiang Province Administration of Traditional Chinese Medicine(China)(No.2024ZL249).
文摘Adipose tissue fibrosis,characterized by abnormal extracellular matrix deposition within adipose tissue,signifies a crucial indicator of adipose tissue malfunction,potentially leading to organ tissue dysfunction.Various factors,including a high-fat diet,non-alcoholic fatty liver disease,and insulin resistance,coincide with adipose tissue fibrosis.MicroRNAs(miRNAs)represent a class of small non-coding RNAs with significant influence on tissue fibrosis through diverse signaling pathways.For instance,in response to a high-fat diet,miRNAs can modulate signaling pathways such as TGF-b/Smad,PI3K/AKT,and PPAR-g to impact adipose tissue fibrosis.Furthermore,miRNAs play roles in inhibiting fibrosis in different contexts:sup-pressing corneal fibrosis via the TGF-b/Smad pathway,mitigating cardiac fibrosis through the VEGF signaling pathway,reducing wound fibrosis via regulation of the MAPK signaling pathway,and diminishing fibrosis post-fat transplantation via involvement in the PDGFR-b signaling pathway.Notably,the secretome released by miRNA-transfected adipose-derived stem cells facilitates targeted delivery of miRNAs to evade host immune rejection,enhancing their anti-fibrotic efficacy.Hence,this study endeavors to elucidate the role and mechanism of miR-NAs in adipose tissue fibrosis and explore the mechanisms and advantages of the secretome released by miRNA-transfected adipose-derived stem cells in combating fibrotic diseases.
