Calcium-dependent protein kinases(CPKs),the best-characterized calcium sensors in plants,regulate many aspects of plant growth and development as well as plant adaptation to biotic and abiotic stresses.However,how CPK...Calcium-dependent protein kinases(CPKs),the best-characterized calcium sensors in plants,regulate many aspects of plant growth and development as well as plant adaptation to biotic and abiotic stresses.However,how CPKs regulate the antioxidant defense system remains largely unknown.We previously found that impaired function of OsCPK12 leads to oxidative stress in rice,with more H_(2)O_(2),lower catalase(CAT)activity,and lower yield.Here,we explored the roles of OsCPK12 in oxidative stress tolerance in rice.Our results show that OsCPK12 interacts with and phosphorylates OsCATA and OsCATC at Ser11.Knockout of either OsCATA or OsCATC leads to an oxidative stress phenotype accompanied by higher accumulation of H_(2)O_(2).Overexpression of the phosphomimetic proteins OsCATAS11D and OsCATCS11D in oscpk12-cr reduced the level of H2O2 accumulation.Moreover,OsCATAS11D and OsCATCS11D showed enhanced catalase activity in vivo and in vitro.OsCPK12-overexpressing plants exhibited higher CAT activity as well as higher tolerance to oxidative stress.Ourndings demonstrate that OsCPK12 affects CAT enzyme activity by phosphorylating OsCATA and OsCATC at Ser11 to regulate H2O2 homeostasis,thereby mediating oxidative stress tolerance in rice.展开更多
基金supported by grants from the National Natural Science Foundation of China (#32100224 and#31961143016)the Science and Technology Program of Zhejiang Province,China (2022R51009)+2 种基金the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2013-CNRRI)the Earmarked Fund for the China Agricultural Research System (CARS-01)the Zhejiang Provincial Natural Science Foundation of China (grant no.LY23C130003).No conflict of interest is declared.
文摘Calcium-dependent protein kinases(CPKs),the best-characterized calcium sensors in plants,regulate many aspects of plant growth and development as well as plant adaptation to biotic and abiotic stresses.However,how CPKs regulate the antioxidant defense system remains largely unknown.We previously found that impaired function of OsCPK12 leads to oxidative stress in rice,with more H_(2)O_(2),lower catalase(CAT)activity,and lower yield.Here,we explored the roles of OsCPK12 in oxidative stress tolerance in rice.Our results show that OsCPK12 interacts with and phosphorylates OsCATA and OsCATC at Ser11.Knockout of either OsCATA or OsCATC leads to an oxidative stress phenotype accompanied by higher accumulation of H_(2)O_(2).Overexpression of the phosphomimetic proteins OsCATAS11D and OsCATCS11D in oscpk12-cr reduced the level of H2O2 accumulation.Moreover,OsCATAS11D and OsCATCS11D showed enhanced catalase activity in vivo and in vitro.OsCPK12-overexpressing plants exhibited higher CAT activity as well as higher tolerance to oxidative stress.Ourndings demonstrate that OsCPK12 affects CAT enzyme activity by phosphorylating OsCATA and OsCATC at Ser11 to regulate H2O2 homeostasis,thereby mediating oxidative stress tolerance in rice.
