Geranylgeranyl pyrophosphate synthetase(GGPPS) has gained increasing attention as a key enzyme in terpene analysis.We designed specific primers based on plant GGPPS homologs and used reverse transcription polymerase c...Geranylgeranyl pyrophosphate synthetase(GGPPS) has gained increasing attention as a key enzyme in terpene analysis.We designed specific primers based on plant GGPPS homologs and used reverse transcription polymerase chain reaction(RT-PCR) to obtain and identify Pin GGPPS,a GGPPS gene sequence from Pinus massoniana,using bioinformatics tools.Quantitative PCR analysis of Pin GGPPS expression levels in roots,pine needles,immature stems,and semilignified stems from 6-month-old P.massoniana showed that expression levels of Pin GGPPS were highest in pine needles,followed by immature stems and semilignified stems,and lowest in roots.When we examined the correlation between Pin GGPPS gene expression levels and resin productivity in 20 adult plants for 28 successive days,Pin GGPPS expression levels presented a substantially linear distribution when plotted against their corresponding resin yields.In summary,we characterized the gene Pin GGPPS for the first time in P.massoniana,and established a correlation between Pin GGPPS gene expression levels and resin productivity,suggesting the importance of theory and production practice for P.massoniana.展开更多
Continuous-cropping(CC)obstacles are among the primary factors limiting the development of global agri-culture.Although most plants are negatively affected by barriers that develop with CC,they may also over-come such...Continuous-cropping(CC)obstacles are among the primary factors limiting the development of global agri-culture.Although most plants are negatively affected by barriers that develop with CC,they may also over-come such obstacles by altering the soil biological and chemical environment to favor plant growth.In this study,we investigated the mechanism by which plants recruit beneficial microorganisms using root exu-dates to alleviate obstacles in a 10-year CC potato system.On day 20 after potato emergence,soil micro-organisms in the CC system promoted an increase in adventitious root(AR)numbers by increasing the indole-3-acetic acid(IAA)content of the rhizosphere soil.Analysis of rhizosphere bacterial communities using 16S rRNA sequencing revealed that CC alters community structure,increasing the abundance of Pantoea sp.MCC16.Irrigation with root exudates from CC potato significantly increased AR numbers and Pantoea sp.MCC16 abundance.Through untargeted metabolomic analysis,we identified nobiletin as key metabolite that promotes Pantoea sp.MCC16 colonization in the rhizosphere.Furthermore,appli-cation of either nobiletin or Pantoea sp.MCC16 significantly improved the yield of CC potatoes.These find-ings demonstrate that CC plants can actively secrete the unique metabolite nobiletin to recruit Pantoea sp.MCC16,a high IAA producer,to help plants recover functional traits and mitigate CC obstacles.展开更多
As the COVID-19 pandemic evolves and new variants emerge,the development of more efficient identification approaches of variants is urgent to prevent continuous outbreaks of SARS-CoV-2.Field-effect transistors(FETs)wi...As the COVID-19 pandemic evolves and new variants emerge,the development of more efficient identification approaches of variants is urgent to prevent continuous outbreaks of SARS-CoV-2.Field-effect transistors(FETs)with two-dimensional(2D)materials are viable platforms for the detection of virus nucleic acids(NAs)but cannot yet provide accurate information on NA variations.Herein,2D Indium selenide(InSe)FETs were used to identify SARSCoV-2 variants.The device's mobility and stability were ensured by atomic layer deposition(ALD)of Al_(2)O_(3).The resulting FETs exhibited sub-fM detection limits ranging from 10^(–14)M to 10^(–8)M.The recognition of single-nucleotide variations was achieved within 15 min to enable the fast and direct identification of two core mutations(L452R,R203M)in Delta genomes(p<0.01).Such capability originated from the trap states in oxidized InSe(InSe_(1-x)O_(x))after ALD,resulting in traps-involved carrier transport responsive to the negative charges of NAs.In sum,the proposed approach might highly provide epidemiological information for timely surveillance of the COVID pandemic.展开更多
基金supported by the Guangxi Natural Science Foundation(2014GXNSFBA118106)
文摘Geranylgeranyl pyrophosphate synthetase(GGPPS) has gained increasing attention as a key enzyme in terpene analysis.We designed specific primers based on plant GGPPS homologs and used reverse transcription polymerase chain reaction(RT-PCR) to obtain and identify Pin GGPPS,a GGPPS gene sequence from Pinus massoniana,using bioinformatics tools.Quantitative PCR analysis of Pin GGPPS expression levels in roots,pine needles,immature stems,and semilignified stems from 6-month-old P.massoniana showed that expression levels of Pin GGPPS were highest in pine needles,followed by immature stems and semilignified stems,and lowest in roots.When we examined the correlation between Pin GGPPS gene expression levels and resin productivity in 20 adult plants for 28 successive days,Pin GGPPS expression levels presented a substantially linear distribution when plotted against their corresponding resin yields.In summary,we characterized the gene Pin GGPPS for the first time in P.massoniana,and established a correlation between Pin GGPPS gene expression levels and resin productivity,suggesting the importance of theory and production practice for P.massoniana.
基金supported by the Natural Science Foundation of Si-chuan Province(grant number 2022NSFSC0014)the Tackling Key Prob-lems and Supporting Projects of Breeding in Sichuan Province(grant numbers 2021YFYZ0019 and 2021YFYZ0005)+1 种基金the Sichuan Potato Inno-vation Team(grant number sccxtd-2023-09)the National Natural Science Foundation of China(grant number 32060443).
文摘Continuous-cropping(CC)obstacles are among the primary factors limiting the development of global agri-culture.Although most plants are negatively affected by barriers that develop with CC,they may also over-come such obstacles by altering the soil biological and chemical environment to favor plant growth.In this study,we investigated the mechanism by which plants recruit beneficial microorganisms using root exu-dates to alleviate obstacles in a 10-year CC potato system.On day 20 after potato emergence,soil micro-organisms in the CC system promoted an increase in adventitious root(AR)numbers by increasing the indole-3-acetic acid(IAA)content of the rhizosphere soil.Analysis of rhizosphere bacterial communities using 16S rRNA sequencing revealed that CC alters community structure,increasing the abundance of Pantoea sp.MCC16.Irrigation with root exudates from CC potato significantly increased AR numbers and Pantoea sp.MCC16 abundance.Through untargeted metabolomic analysis,we identified nobiletin as key metabolite that promotes Pantoea sp.MCC16 colonization in the rhizosphere.Furthermore,appli-cation of either nobiletin or Pantoea sp.MCC16 significantly improved the yield of CC potatoes.These find-ings demonstrate that CC plants can actively secrete the unique metabolite nobiletin to recruit Pantoea sp.MCC16,a high IAA producer,to help plants recover functional traits and mitigate CC obstacles.
基金This work was financially supported by the National Natural Science Foundation of China(92064007,62001224,52131304)the Natural Science Foundation of Jiangsu Province(BK20190476,BK20190457,BZ2020063)the 69th batch of China Postdoctoral Science Foundation(2021M691600).
文摘As the COVID-19 pandemic evolves and new variants emerge,the development of more efficient identification approaches of variants is urgent to prevent continuous outbreaks of SARS-CoV-2.Field-effect transistors(FETs)with two-dimensional(2D)materials are viable platforms for the detection of virus nucleic acids(NAs)but cannot yet provide accurate information on NA variations.Herein,2D Indium selenide(InSe)FETs were used to identify SARSCoV-2 variants.The device's mobility and stability were ensured by atomic layer deposition(ALD)of Al_(2)O_(3).The resulting FETs exhibited sub-fM detection limits ranging from 10^(–14)M to 10^(–8)M.The recognition of single-nucleotide variations was achieved within 15 min to enable the fast and direct identification of two core mutations(L452R,R203M)in Delta genomes(p<0.01).Such capability originated from the trap states in oxidized InSe(InSe_(1-x)O_(x))after ALD,resulting in traps-involved carrier transport responsive to the negative charges of NAs.In sum,the proposed approach might highly provide epidemiological information for timely surveillance of the COVID pandemic.
